jm calibrators:...5 cal 5 p1442 blood opiates.m cal 5.d calibration 2 6 cal 25 p1 -a3 blood...

BLOOD OPIATESCONTROL PACKET CHECKLIST

Highlighted areas are to be comPlpted by analyst '

Batch ID Number: 12 -Aug -16

Analyst: JM

REAGENTS: Lot #

Calibrators • 042815Controls 091615

Internal Standard 070215Blank Blood 031516

SPE Columns 151014S-1Carbonate Buffer pH 9.0 042116

0.1 M HCI 042116Methanol 157966

Ethyl Acetate 138711DCM 127997IPA 138899

NH4OH 146981Acetonitrile (HPLC) 156997

H20 (HPLC) 158398Methanol (HPLC) 154246

Formic Acid 152755

Extraction Date 08/12/16Instrument Log Completed • yes

Last Pipette Precision Check 6/28/16

GENERAL:

1 ---SEQUENCE TABLE INCLUDED

Technically Reviewed by:

Date:

CALIBRATORS: +/- 20% ion ratio and quant must be metLEVEL Mor Cod . HM OM HC OC 6AM2 . / - ,..- .,...- ....-‘-

. .

5 r - — --- ---- ..--- ••••"" ......

25100

-... . . — ...- - - ......--

250 . ' ...." ' ..- -." , - - , - - -

lila calibration level has been eliminated from the curve , 3

,explain below • ' _ , •

Plpettors UsedPurpose Volume Serlal#

Cal/QC L100 D0993080A

Sample L1000 G0638621A

Internal Standard Repeater 4115795

CAL CURVE PRESENT / >0.98 ALL ANALYTES

INITIALS ON ALL PAGES

QUALITY CONTROL ENTERED INTO LOG

BATCH ACCEPTED / QC LOG INITIALED

, ..„--tNSTRUMENT LOG REVIEWED

ION RATIO ACCEPT CRITERIA SET AT 20% RT WINDOWS (± 5%) OR BETTER

COMMENTS:

Sample

Name .Data File Type Level Acq. Method File Acq: Date -Time

SB before Batch... SB before Batch... I DoubleBlank Blood Opiates.m 18/12/2016 11:1...

Quantifier

Name TS Transition Scan Type RT Window RT Units

Mor-D6 1 292.2-> 152.1 MRM ISTD 10.000 Percent

Morph (C0-10) 1 286.2 -> 152.1 Target 10.000 Percent

OxyMor-D3 11 305.2-> 287.1_MRMMRM ISTD 10.000 Percent

OxMor 1 302.2 -> 284.0 MRM Target 10.000 Percent

HyMor-D3 1 289.2-> 185.0 MRM ISTD 10.000 Percent

HyMor 1 286.2-> 185.0 MRM Target 10.000 Percent

Cod -D6 1 306.2-> 115.0 MRM ISTD 10.000 Percent

Cod (C0-2) 1 300.2-> 115.0 MRM Target 10.000 Percent

OxCod-D3 1 319.2 ->301.1 MRM ISTD 10.000 Percent

OxCod 1 316.2 -> 298.2 MRM Target 10.000 Percent

HyCod-d3 1 303.2-> 199.0 MRM ISTD 10.000 Percent

HyCod 1 300.2-> 199.0 MRM Target 10.000 Percent

6 -AM -d3 1 331.2-> 165.0 MRM ISTD 10.000 Percent

6 -AM (C0-2) 1 328.2-> 165.0 MRM Target 10.000 Percent

Sample

. Name Data File Type Level I Acq. Method File I Acq. Date -Time

SB before Batch... I SB before Batch... I DoubleBlank Blood Opiates.m 18/1212016 11:1...

Quantifier

Name TS Transition Scan Type Uncertainty

Mor-D6 1 1 292.2 -> 152.1

_

MRM ISTD

_

Relative

Qualifier

Precursor Ion Product Ion Transition Rel. Resp. Uncertainty

292.2 128.0 292.2-> 128.0 60.31 20.0

Quantifier

Name TS Transition Scan Type Uncertainty

Morph (C0-10) 1 286.2-> 152.1 MRM Target Relative

Qualifier


286.21 128.0 286.2 ->128.0 63.5 20.0

Quantifier

Name TS Transition ' Scan Type Uncertainty

OxyMor-D3 1 305.2 ->287.1 MRM ISTD Relative

Qualifier_--


305.2 230.1 305.2 ->230.1 36.4 20.0

Quantifier

Name TS-

Transition • ' . Scan,,•,' Type Uncertainty h

OxMor I 1 302.2 -> 284.0 MRM Target Relative

Qualifier .

Precursor Ion Product Ion ' Transition Rel. Resp. Uncertainty

302.2 227.1 302.2-> 227.1 35.2 20.0

Quantifier _

Name TS Transition.

Scan_" Type Uncertainty

HyMor-D3 1 1 289.2 -> 185.0 MRM ISTD Relative

Qualifier 2

..Precursor Ion Product Ion Transition Rel. Resp. '. Uncertainty

289.2 157.0 289.2 -> 157.0 73.3 20.0

Quantifier -

- Name TS Transition Scan - ' J.:' r Type Uncertainty

HyMor 1 1 286.2-> 185.0 MRM Target Relative

Qualifier

Precursor Ion Product Ion I Transition I Rel. Resp. I Uncertainty

1 286.2 157.01 286.2-> 157.0 67.41 20.0

Quantifier•

Name TS Transition Scan - Type Uncertainty

Cod -D6 I 1 306.2-> 115.0 MRM ISTD Relative

Qualifier

'Precursor Ion ' Product Ion Transition Rel: Resp. Uncertainty

306.2 152.0 306.2-> 152.0 118.81 20.0

Quantifier _

Name I S Transition Scan Type Uncertainty

Cod (C0-2) 1 300.2-> 115.0 MRM Target Relative

Qualifier


300.2 152.0 300.2-> 152.0 106.8 20.0

Quantifier

Name . - TS Transition Scan - Uncertainty

OxCod-D3 1 319.2 -> 301.1 MRM ISTD Relative

77 •,.Qualifier ' .. - . V.-


319.2 244.0 319.2 -> 244.0 1 20.7 20.07

Quantifier _

• , Name '''' : .. TS.

Transition. .Scan...

' Type _ Uncertainty

OxCod 1 316.2 -> 298.2 MRM Target Relative

Qualifier . - ...


316.2 241.0 316.2 -> 241.0 20.31 20.0

Quantifier

Name .• '• , 1 TS Transition . ' Scan Type Uncertainty

HyCod-d3 1 303.2-> 199.0 MRM I ISTD Relative

Qualifier -• ' '' " ,

Precursor Ion Product Ion Transition Rel. Resp. • Uncertainty

303.2 171.01 303.2-> 171.0 40.01 20.0

Quantifier. 6; ::. i

Name ,' „ 'TS Transition Scan Type Uncertainty

HyCod 1 300.2 -> 199.0 1 MRM Target Relative

Qualifier.


300.2 128.0 300.2 -> 128.0 44.9 20.0

Quantifier

Name TS Transition Scan Type _ Uncertainty

6 -AM -d3 1 331.2-> 165.0 MRM ISTD Relative

Qualifier_Precursor Ion Product Ion Transition Ftel. Resp. Uncertainty

331.2 152.0 331.2-> 152.0 1 45.5 20.0

Quantifier

Name TS Transition 1 Scan Type Uncertainty

6 -AM (C0-2) 1 328.2-> 165.0 MRM Target 1 Relative.. .

Qualifier .....

Precursor Ion. _ Product Ion - Transition Rel. Resp. Uncertainty

328.21 211.0 328.2 -> 211.0 1 50.0 20.0

Worklist Report Agilent Technologies

Instrument Name: 6460-6 Worklist Path: D:12016 DataIJMIOPIATESISequences1081216bIdopiate.wkl

Operator Name: JMRun Type: Standard Start

Part of Method to Run: Acquisition Only

Execution of Acquisition -DA: Synchronous

Acquisition Method Path: DIMassHunterlmethods

DA Method Path: DIMassHuntemethods

Data File Path: D:12016 DataIJMIOPIATES1081216

Pre-Worklist Script:

Post-Worklist Script: SCP_InstrumentStandbyQ{MH_Acq_Scripts.exe}

Acquisition Clean Up Script: ---

Overlapped Injection: Yes

Clear Sample Selection After Run: Yes

Wait Time for Ready(Min): 10Threshold Disk Value(GB): 10Comment:

Plate Barcode: Plate

P1Barcode

Worklist Table

Sample Name Comment Sample

Position

Method Data File Sample Type Level Name

1 SB before Batch

1Vial 1 Blood Opiates.m SB before Batch

1.d

DoubleBlank

2 SB before Batch


2.d

DoubleBlank

3 SB before Batch


3.d

DoubleBlank

4 CAL 2 P1 -A1 Blood Oplates.m CAL 2.d Calibration I

5 CAL 5 P1442 Blood Opiates.m CAL 5.d Calibration 2

6 CAL 25 P1 -A3 Blood Opiates.m CAL 25.d Calibration 3



9 SB before

Negative

Vial 1 Blood Opiates.m SB before

Negative.d

DoubleBlank

10 Negative P1 -A6 Blood Opiates.m Negative.d Sample

11 Low Control P1 -Al Blood Opiates.m Low Control.d Sample

D:12016 Data1JMIOPIATESISequences1081216bIdopiate.wkl

Report generation date: 12 -Aug -2016 10:42:35 AM Page 1 of 5

Worklist Report -0.. Agilent Technologies

Sample Name Comment Sample Method Data File Sample Type Level Name

Position

12 SB 2016011176 Vial 1 Blood Opiates.m SB 2016011176 DoubleBlank

1.A - 1.A -.d

13 2016011176 1A P1 -B1 Blood Opiates.m 2016011176 1A Sample

-.d

14 SB 2016705906 Vial 1 Blood Opiates.m SB 2016705906 DoubleBlank•

1211483.A- 1211483.A -.d

15 2016705906 P1 -B2 Blood Opiates.m 2016705906 Sample

1211483.A- 1211483,A -.d

16 SB 2016706633 Mal 1 Blood Opiates.m SB 2016706633 DoubleBlank

TE262830-1A - TE262830-1A

-.d

17 2016706633 P1-133 Blood Opiates.m 2016706633 Sample

.

1E262830 -1A - TE262830-1A

-.d


LA- 1A -.d

19 2016707484 1A P1 -B4 Blood Opiates.m 2016707484 lA Sample

-.d


821 -1 -AA - 821-1-A.A -.d

21 2016707760 821 P1-65 Blood Opiates.m 2016707760 821 Sample.

-I-AA - -1-AA -.d


1A- 1A -.d1

23 2016026838 I.A P1 -B6 Blood Opiates.m 2016026838 1A Sample

.

• -.d


01.A- 01.A -.d ,25 2016706947 PI -67 Blood Opiates.m 2016706947 Sample

01.A- 01.A -.d


285-1.A - 285 -1A -.d

27 2016707154 285 P1 -B8 Blood Opiates.m 2016707154 285 Sample.

•1.A- -1.A -.d


2.A- 2A -.d

29 20167074172.A P1 -B9 Blood Opiates.m 20167074112.A Sample

-.d


1A - 1A -,d

31 20167074181.A PI -C1 Blood Opiates.m 2016707418 IA Sample

-.d


CH70464-1A - CH70464-1A -.d'33 2016707597 P1 -C2 Blood Opiates.m 2016707597 Sample

CH70464-1.A - CH70464-1A -.d

0:12016 DataliMIOPIATES \Sequences1081216bIdopiate.wkl


Worklist Report, .

Agilent Technologies \


Position


CH70848-1A - CH70848-1.A -.d

35 2016707598 P1 -C3 Blood Opiates.m 2016707598 Sample

CH70848-1A - CH70848-1A -.d


1.A - 1.A -.d

37 2016707708 1A P1 -C4 Blood Opiates.m 2016707708 1A Sample

-.d


2A- 2A -.d

39 20167077752.A P1-05 Blood Opiates.m 20167077752.A Sample

-.d


0007.A- 0007.A -.d

41 2016708210 P1 -C6 Blood Opiates.m 2016708210 Sample

0007.A - 0007.A -.d


200-01.A- 200 -01A -.d

43 2016708347 200 P1 -C7 Blood Opiates.m 2016708347 200 Sample

-01A- -01.A -.d

44 SB Vial 1 Blood Opiates.m SB DoubleBlank

AZ1600006320 AZ1600006320

241k - 24.A -,d

45 AZ1600006320 P1 -C8 Blood Opiates.m AZ1600006320 Sample

24.A. 24.A -.d

46 SB Vial 1 Blood Opiates.m SB DoubleBlank

A11600006929 AZ1600006929

1.A- 1.A -.d

47 AZ1600006929 P1 -C9 Blood Opiates.m AZ1600006929 Sample

1A - 1.A -.d


IA- 1A -.d

49 2016024724 1A P1-01 Blood Opiates.m 2016024724 1.A Sample

-.d

50 SB before Vial 1 Blood Opiates.m SB before DoubleBlank

Medium Control Medium

Control.d

51 Medium Control P1 -A8 Blood Opiates.m Medium Sample

Control.d


1-1.A - 1 -1.A -.d

53 20160255091- P1-02 Blood Opiates.m 20160255091- Sample

IA- 1A -.d


1A - 1.A -.d

D:12016 DataIJMIOPIATESlSequencesl081216bIdopiate.wld


Worklist Report Agilent Techno1ogies


Position

55 2016026327 IA P1-03 Blood Opiates.m 2016026327 1A Sample.

-.d


. .

1.A - 1A -.d

57 2016026545 1A P1-04 Blood Opiates.m 2016026545 1A Sample

-.d

58 SB 2016026971 Vial 1 Blood Opiates.m SB 2016026971 DoubleBlank'

1SJA- 1SJ.A -.d

59 2016026971 P1 -D5 Blood Opiates.m 2016026971 Sample

_

1SJ.A - 1SIA -.d


CH71670-1.A - CH71670-1.A -.d.

61 2016708312 P1 -D6 Blood Opiates.m 2016708312 Sample

.

CH71670-1A- CH71670-1.A-.d


6RM A - 6RMA -.d

63 2016708396 P1-07 Blood Opiates.m 2016708396 Sample

6RM.A - 6RM.A -.d

64 SB 2016708450 Vial 1 Blood Opiates.m SB 2016708450 DoubleBlank,

1A- 1.A -.d

65 2016708450 1A P1 -D8 Blood Opiates.m.2016708450 1.A Sample

•

• -.d_66 SB 2016708468 Vial 1 Blood Opiates.m SB 2016708468 DoubleBlank

IA- 1,A -.d

67 2016708468 lA P1-09 Blood Opiates.m 2016708468 1.A Sample.

-.d


41603A- 41603.A -.d

69 2016708665 P1 -E1 Blood Opiates.m 2016708665 Sample,

41603.A- 41603.A -.d


1.A - 1A -.d

71 2016708744 1A P1 -E2 Blood Opiates.m 2016708744 1A Sample

-.d


1A- 1.A -.d

73 2016709020 1A P1 -E3 Blood Opiates.m 2016709020 1A Sample

.

-.d

74 SB 2016709036 Vial 1 Blood Opiates.m SB 2016709036 DoubleBlank.

1001 -1 -NA- 1001 -1 -AA -.d

75 2016709036 P1 -E4 Blood Opiates.m 2016709036 Sample-

1001 -1 -AA- 1001 -1 -AA -.d'76 SB Vial 1 Blood Opiates.m SB DoubleBlank

AZ1600007447 AZ1600007447

AJ1-1A - AJ1-1A -.d

D:12016 DataIJMIOPIATESISequences1081216bIdopiate.wkl


Worklist Report Agilent Technologies•


Position

77 A21600007447 P1 -E5 Blood Opiates.m AZ1600007447 Sample

AJ1-1.A - AJ1-1A -.d


100.A- 100.A -.d

79 2016712064 P1 -E6 Blood Opiates.m 2016712064 Sample

100.A- 100.A -.d

80 SB before High Vial 1 Blood Opiates.m SB before High DoubleBlank

Control Control.d

81 High Control P1 -A9 Blood Opiates.m High Control.d Sample

82 Final Solvent Vial 1 Blood Opiates.m Final Solvent DoubleBlank

Blank Blank.d

83 SCP_Instrument

Standby()

(MH_Acq_Script

s.exe)

D:12016 DataIJM1OPIATES \Sequences1081216b1dopiate.wkl


Quant Summary Report (ISTD)

Batch Info

Batch Data PathAnalysis TimeReport TimeLast Calib Update

D:\2016 DataVM \OPIATES\081216\QuantResults\081216jm.batch.bin8/15/2016 6:12 AM8/15/2016 6:13 AM8/15/2016 6:12 AM

Sequence Table

Sample NameSB before Batch 1

SB before Batch 2

SB before Batch 3

CAL 2

CAL 5

CAL 25

CAL 100

CAL 250

SB before Negative

Negative

Low Control

SB 2016011176 LA -

2016011176 1.A -

SB 2016705906 1211483.A -

2016705906 1211483.A -

SB 2016706633 TE262830-1.A -

2016706633 TE262830-1.A -

SB 2016707484 1.A -

2016707484 1.A -

SB 2016707760 821-1-A.A -

2016707760 821-1-A.A -

SB 2016026838 1.A -

2016026838 1.A -

SB 2016706947 01.A -

2016706947 01.A -

SB 2016707154 285-1.A -

2016707154 285 -LA -

SB 2016707417 2.A -

20167074172.A -

SB 2016707418 1.A -

2016707418 1.A -

SB 2016707597 0170464-1.A -

2016707597 0 -170464 -LA -

Data FileSB before Batch 1.d

SB before Batch 2.d

SB before Batch 3.d

CAL 2.d

CAL 5.d

CAL 25.d

CAL 100.d

CAL 250.d

SB before Negative.d

Negative.d

Low Conti-01.d

SB 2016011176 1.A -.d

2016011176 1.A -.d

SB 2016705906 1211483.A -.d

2016705906 1211483.A -.d

SB 2016706633 TE262830-1.A -.d

2016706633 TE262830-1.A -.d

SB 2016707484 1.A -.d

2016707484 1.A -.d

SB 2016707760 821-1-A.A -.d

2016707760 821-1-A.A -.d

SB 2016026838 1.A -.d

2016026838 1.A -.d

SB 2016706947 01.A -.d

2016706947 01.A -.d

SB 2016707154 285 -LA -.d

2016707154 285 -1.A -.d

SB 2016707417 2.A -.d

2016707417 2.A -.d

SB 2016707418 1.A -.d

2016707418 1.A -.d

SB 2016707597 0-170464-1.A -.d

2016707597 0170464 -LA -.d

Sample Type Position Date & TimeDoubleBlank Vial 1 8/12/2016 10:47 AMDoubleBlank Vial 1 8/12/2016 11:01 AMDoubleBlank Vial 1 8/12/2016 11:15 AMCalibration PI -Al 8/12/2016 11:29 AMCalibration PI -A2 8/12/2016 11:42 AMCalibration P1 -A3 8/12/2016 11:56 AMCalibration P1 -A4 8/12/2016 12:10 PM

Calibration P1-A5 8/12/2016 12:23 PM

DoubleBlank Vial 1 8/12/2016 12:37 PM

Sample P1 -A6 8/12/2016 12:51 PM

Sample P1 -A7 8/12/2016 1:05 PM


Sample P1-61 8/ 12/2016 1:32 PM

DoubleBlank Vial 1 8/12/2016 1:46 PMSample PI -82 8/12/2016 2:00 PM

DoubleBlank Vial 1 8/12/2016 2:13 PMSample PI -63 8/12/2016 2:27 PMDoubleBlank Vial 1 8/12/2016 2:41 PM

Sample P1-84 8/12/2016 2:54 PM


Sample P1-65 8/ 12/2016 3:22 PM


Sample P1-136 8/12/2016 3:49 PM


Sample P1-137 8/ 12/2016 4:17 PM


Sample P1-68 8/12/2016 4:44 PMDoubleBlank Vial 1 8/12/2016 4:58 PMSample P1-69 8/12/2016 5:12 PM

DoubleBlank Vial 1 8/12/2016 5:25 PMSample P1 -C1 8/12/2016 5:39 PM


Sample 13142 8/12/2016 6:07 PM

Inj. Volume

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

Arizona Department of Public Safety Page 1 of 194 Printed at: 6:19 AM on: 8/15/2016


SB 2016707598 CH70848-1.A - SB 2016707598 0170848-1.A -.d DoubleBlank Vial 1 8/12/2016 6:20 PM -1.00



Sample Name Data File Sample Type Position Date & Time Inj. Volume

2016707598 CH70848-1.A - 2016707598 CH70848-1.A -.d Sample P1-0 8/12/2016 6:34 PM -1.00

SB 2016707708 LA - SB 2016707708 1.A -.d DoubleBlank Vial 1 8/12/2016 6:48 PM -1.00

2016707708 1.A - 2016707708 1.A -.d Sample P1 -C4 8/12/2016 7:02 PM -1.00

SB 2016707775 2.A - SB 2016707775 2.A -.d DoubleBlank Vial 1 8/12/2016 7:15 PM -1.00

2016707775 2.A - 2016707775 2.A -.d Sample P1-0 8/12/2016 7:29 PM -1.00


2016708210 0007.A - 2016708210 0007.A -.d Sample 1,1-0 8/12/2016 7:56 PM -1.00

SB 2016708347 200-01.A - SB 2016708347 200-01.A -.d DoubleBlank Vial 1 8/12/2016 8:10 PM -1.00

2016708347 200-01.A - 2016708347 200-01.A -.d Sample P1 -C7 8/12/2016 8:24 PM -1.00

SB AZ1600006320 24.A - SB AZ1600006320 24.A -.d DoubleBlank Vial 1 8/12/2016 8:38 PM -1.00

AZ1600006320 24.A - AZ1600006320 24.A -.d Sample P1-0 8/12/2016 8:51 PM -1.00

SB AZ1600006929 1.A - SB AZ1600006929 1.A -.d DoubleBlank Vial 1 8/12/2016 9:05 PM -1.00

A21600006929 1.A - AZ1600006929 1.A -.d Sample PI -C9 8/12/2016 9:19 PM -1.00

SB 2016024724 LA - SB 2016024724 1.A -.d DoubleBlank Vial 1 8/12/2016 9:33 PM -1.00

2016024724 1.A - 2016024724 1.A -.d Sample P1 -D1 8/12/2016 9:46 PM -1.00

SB before Medium Control SB before Medium Control.d DoubleBlank Vial 1 8/12/2016 10:00 PM -1.00

Medium Control Medium Control.d Sample PI -A8 8/12/2016 10:14 PM -1.00

SB 2016025509 I -1.A - SB 2016025509 1-1.A -.d DoubleBlank Vial 1 8/12/2016 10:27 PM -1.00

2016025509 1-1.A - 2016025509 1-I.A -.d Sample P1-1)2 8/ 12/2016 10:41 PM -1.00


2016026327 1.A - 2016026327 1.A -.d Sample P1 -D3 8/12/2016 11:09 PM -1.00


2016026545 1.A - 2016026545 1.A -.d Sample P1 -N 8/12/2016 11:36 PM -1.00

SB 2016026971 1SJ.A - SB 2016026971 1SJ.A -.d DoubleBlank Vial 1 8/ 12/2016 11:50 PM -1.00

2016026971 1SJ.A - 2016026971 1SJ.A -.d Sample P1 -D5 8/13/2016 12:03 AM -1.00

SB 2016708312 0 -171670 -LA - SB 2016708312 0-171670-1.A -.d DoubleBlank Vial 1 8/13/2016 12:17 AM -1.00

2016708312 CH71670-1.A - 2016708312 0H71670 -LA -.d Sample P1-1)6 8/13/2016 12:31 AM -1.00

SB 2016708396 6RM.A - . SB 2016708396 6RM.A -.d DoubleBlank Vial 1 8/13/2016 12:45 AM -1.00

2016708396 6RM.A - 2016708396 6RM.A -.d Sample P1 -D7 8/13/2016 12:58 AM -1.00

SB 2016708450 1.A - SB 2016708450 1.A -.d DoubleBlank Vial 1 8/13/2016 1:12 AM -1.00

2016708450 1.A - 2016708450 1.A -.d Sample P1 -D8 8/ 13/2016 1:26 AM -1.00

SB 2016708468 1.A - SB 2016708468 1.A -.d DoubleBlank Vial 1 8/ 13/2016 1:39 AM -1.00

2016708468 1.A - 2016708468 1.A -.d Sample P1-09 8/13/2016 1:53 AM -1.00


2016708665 41603.A - 2016708665 41603.A -.d Sample 131 -El 8/13/2016 2:21 AM -1.00


2016708744 1.A - 2016708744 1.A -.d Sample P1 -E2 8/13/2016 2:48 AM -1.00


2016709020 1.A - 2016709020 1.A -.d Sample P1-E3 8/13/2016 3:15 AM -1.00

SB 2016709036 1001-1-A.A - SB 2016709036 1001-1-A.A -.d DoubleBlank Vial 1 8/13/2016 3:29 AM -1.00

2016709036 1001-1-A.A - 2016709036 1001-1-A.A -.d Sample P1 -E4 8/13/2016 3:43 AM -1.00



SB AZ1600007447 Al1-1.A - SB AZ1600007447 Al1-1.A -.d DoubleBlank Vial 1 8/13/2016 3:57 AM -1.00



Sample NameAZ1600007447 Al1-1.A -

SB 2016712064 100.A -

2016712064 100.A -

SB before High Control

High Control

Final Solvent Blank

Quantitation Results

Data File

SB before Batch 1.d

SB before Batch 1.d

SB before Batch 1.d

SB before Batch 1.d

SB before Batch 1.d

SB before Batch 1.d

SB before Batch 1.d

Data File

SB before Batch 2.d

SB before Batch 2.d

SB before Batch 2.d

SB before Batch 2.d

SB before Batch 2.d

SB before Batch 2.d

SB before Batch 2.d

Data FileSB before Batch 3.d

SB before Batch 3.d

SB before Batch 3.d

SB before Batch 3.d

SB before Batch 3.d

SB before Batch 3.d

SB before Batch 3.d

Data File

CAL 2.d

CAL 2.d

CAL 2.d

CAL 2.d

CAL 2.d

CAL 2.d

CAL 2.d

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Data FileAZ1600007447 A.11 -1.A -.d

SB 2016712064 100.A -.d

2016712064 100A -.d

SB before High Control.d

High Control.d

Final Solvent Blank.d

Compound

Morph (CO -W)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Exp Corm

Exp Conc

Exp Conc

Sample Type

SampleDoubleBlank

Sample

DoubleBlank

Sample

DoubleBlank

Response

Response

Response

Position

P1 -E5

Vial 1

P1 -E6

Vial 1

PI -A9

Vial 1

Date & Time

8/13/2016 4:10 AM8/13/2016 4:24 AM8/13/2016 4:38 AM8/13/2016 4:51 AM8/13/2016 5:05 AM8/13/2016 5:19 AM

ISTD Resp Resp Ratio Accuracy

Inj. Volume

-1.00

-1.00

-1.00

-1.00

-1.00

-1.00

Concentration

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ISTD Resp Resp Ratio Accuracy Concentration

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml


ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

Exp Conc Response ISTD Resp Resp Ratio Accuracy

2.00 762 38741 0.0197 102.6

2.00 2035 123373 0.0165 103.7

2.00 1515 98772 0.0153 100.8

2.00 1131 51655 0.0219 99.8

2.00 4559 210933 0.0216 101.3

2.00 2706 131943 0.0205 98.0

2.00 1946 107738 0.0181 100.5

Concentration

2.05 ng/ml

2.07 ng/ml

2.02 ng/ml

2.00 ng/ml

2.03 ng/ml

1.96 ng/ml

2.01 ng/ml



Data File Compound ISTD Exp Conc Response ISTD Resp Resp Ratio Accuracy Concentration

CAL 5.d Morph (C0-10) Mor-D6 5.00 2430 41701 0.0583 99.2 4.96 ng/ml

CAL 5.d OxMor OxyMor-D3 5.00 5522 129776 0.0425 98.4 4.92 ng/ml

CAL 5.d FlyMor HyMor-D3 5.00 4280 100679 0.0425 101.4 5.07 ng/ml

CAL 5.d Cod (C0-2) Cod -D6 5.00 3144 52766 0.0596 101.1 5.05 ng/ml

CAL 5.d OxCod OxCod-D3 5.00 11844 220152 0.0538 100.9 5.04 ng/ml

CAL 5.d HyCod FlyCod-d3 5.00 7637 136189 0.0561 102.5 5.13 ng/ml

CAL 5.d 6 -AM (C0-2) 6 -AM -d3 5.00 5550 111619 0.0497 101.1 5.05 ng/ml




CAL 25.d HyMor HyMor-D3 25.00 22163 103563 0.2140 96.9 24.22 ng/ml



CAL 25.d HyCod HyCod-d3 25.00 39306 140671 0.2794 99.4 24.84 ng/ml

CAL 25.d 6 -AM (C0-2) 6 -AM -d3 25.00 28647 113376 0.2527 97.8 24.44 ng/ml


CAL 100.d Morph (CO -W) Mor-D6 100.00 53610 39464 1.3584 100.9 100.91 ng/ml






CAL 100.d 6 -AM (C0-2) 6 -AM -d3 100.00 116109 108255 1.0725 100.7 100.70 ng/ml








CAL 250.d 6 -AM (C0-2) 6 -AM -d3 250.00 278142 100222 2.7753 99.9 249.79 ng/ml


SB before Negative.d Morph (CO -10) Mor-D6 ND ng/ml

SB before Negative.d OxMor OxyMor-D3 ND ng/ml

SB before Negative.d HyMor HyMor-D3 ND ng/ml

SB before Negative.d Cod (C0-2) Cod -D6 ND ng/ml

SB before Negative.d OxCod OxCod-D3 ND ng/ml

SB before Negative.d HyCod HyCod-d3 ND ng/ml

SB before Negative.d 6 -AM (C0-2) 6 -AM -d3 ND ng/ml




Negative.d Morph (C0-10) Mor-D6 43693 ND ng/ml

Negative.d OxMor OxyMor-D3 135595 ND ng/ml

Negative.d HyMor HyMor-D3 105321 ND ng/ml

Negative.d Cod (C0-2) Cod -D6 55201 ND ng/ml

Negative.d OxCod OxCod-D3 879 228469 0.0038 0.36 ng/ml

Negative.d HyCod HyCod-d3 143298 ND ng/ml

Negative.d 6 -AM (C0-2) 6 -AM -d3 116578 ND ng/m1


Low Control.d Morph (C0-10) Mor-D6 5095 41659 0.1223 9.78 ng/ml

Low Control.d OxMor OxyMor-D3 11050 130803 0.0845 9.49 ng/ml

Low Control.d HyMor HyMor-D3 8422 101264 0.0832 9.63 ng/ml

Low Control.d Cod (C0-2) Cod -D6 6164 52496 0.1174 9.75 ng/ml

Low Control.d OxCod OxCod-D3 22260 209884 0.1061 9.94 ng/ml

Low Control.d HyCod HyCod-d3 14346 136348 0.1052 9.49 ng/ml

Low Control.d 6 -AM (C0-2) 6 -AM -d3 10758 110078 0.0977 9.66 ng/ml


SB 2016011176 1.A -.d Morph (CO -10) Mor-D6 ND ng/ml

SB 2016011176 1.A -.d OxMor OxyMor-D3 ND ng/ml

SB 2016011176 1.A -.d HyMor HyMor-D3 ND ng/ml

SB 2016011176 1.A -.d Cod (C0-2) Cod -D6 ND ng/ml

SB 2016011176 1.A -.d OxCod OxCod-D3 ND ng/ml

SB 2016011176 1.A -.d HyCod HyCod-d3 ND ng/ml

SB 2016011176 1.A -.d 6 -AM (C0-2) 6 -AM -d3 ND ng/ml


2016011176 1.A -.d Morph (C0-10) Mor-D6 40469 ND ng/ml

2016011176 1.A -.d OxMor OxyMor-D3 125452 ND ng/ml

2016011176 1.A -.d HyMor HyMor-D3 99618 ND ng/ml •2016011176 1.A -.d Cod (C0-2) Cod -D6 51292 ND ng/ml

2016011176 1.A -.d OxCod OxCod-D3 142466 208224 0.6842 63.57 ng/ml

2016011176 1.A -.d HyCod HyCod-d3 135467 ND ng/ml

2016011176 1.A -.d 6 -AM (C0-2) 6 -AM -d3 108071 ND ng/ml


SB 2016705906 1211483.A -.d Morph (C0-10) Mor-D6 ND ng/ml









Data File2016705906 1211483.A -.d

2016705906 1211483.A -.d

2016705906 1211483.A -.d

2016705906 1211483.A -.d

2016705906 1211483.A -.d

2016705906 1211483.A -.d

2016705906 1211483.A -.d

Data FileSB 2016706633 TE262830-1.A

SB 2016706633 TE262830-1.A

SB 2016706633 TE262830-1.A

SB 2016706633 TE262830-1.A

SB 2016706633 TE262830-1.A

SB 2016706633 TE262830-1.A

SB 2016706633 TE262830-1.A

Data File

2016706633 TE262830-1.A -.d

2016706633 TE262830-1.A -.d

2016706633 TE262830-1.A -.d

2016706633 TE262830-1.A -.d

2016706633 TE262830-1.A -.d

2016706633 TE262830-1.A -.d

2016706633 TE262830-1.A -.d

Data File

SB 2016707484 1.A -.d

SB 2016707484 1.A -.d

SB 2016707484 1.A -.d

SB 2016707484 1.A -.d

SB 2016707484 1.A -.d

SB 2016707484 1.A -.d

SB 2016707484 1.A -.d

Data File

2016707484 1.A -.d

2016707484 1.A -.d

2016707484 1.A -.d

2016707484 1.A -.d

2016707484 1.A -.d

2016707484 1.A -.d

2016707484 1.A -.d

-.d

-.d

-.d

-.d

-.d

-.d

-.d

Compound

Morph (CO -W)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound ISTD

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (00-2)

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Exp Conc Response ISTD Resp Resp Ratio Accuracy

38150

559 121420 0.0046

95990

5041318969 205514 0.0923

133143

106486

Exp Conc

Exp Conc

Exp Conc

Exp Conc

Response

Concentration

ND ng/ml

0.77 ng/ml

ND ng/ml

ND ng/ml

8.65 ng/ml

ND ng/ml

ND ng/ml

ISTD Resp Resp Ratio Accuracy

Response ISTD Resp Resp Ratio Accuracy

326642 25488 12.8155

120597

438 98172 0.0045

909 49474 0.0184

757 205193 0.0037

128778

103083


Response ISTD Resp Resp Ratio

38918

1899 124538 0.0153

96781

50022

52934 202961 0.2608

131518

104972

Concentration

ND ng/m I

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

Concentration

823.11 ng/ml

ND ng/ml

0.79 ng/ml

1.71 ng/ml

0.34 ng/ml

ND ng/ml

ND ng/ml

Concentration

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

Accuracy Concentration

ND ng/ml

1.94 ng/ml

ND ng/ml

ND ng/ml

24.38 ng/ml

ND ng/ml

ND ng/ml




SB 2016707760 821-1-A.A -.d Morph (CO -W) Mor-D6 ND ng/m1

SB 2016707760 821-1-A.A -.d OxMor OxyMor-D3 ND rig/m1

SB 2016707760 821-1-A.A -.d HyMor HyMor-D3 ND NMI!SB 2016707760 821-1-A.A -.d Cod (C0-2) Cod -D6 ND rig/ml

SB 2016707760 8214-A.A -.d OxCod OxCod-D3 ND ng/ml

SB 2016707760 821-1-A.A -.d HyCod HyCod-d3 ND rig/ml

SB 2016707760 821-1-A.A -.d 6 -AM (C0-2) 6 -AM -d3 ND rig/ml


2016707760 821-1-A.A -.d Morph (C0-10) Mor-D6 40761 ND Wm?

2016707760 821-1-A.A -.d OxMor OxyMor-D3 129180 ND ng/ml

2016707760 821-1-A.A -.d HyMor HyMor-D3 99795 ND rig/ml

2016707760 821-1-A.A -.d Cod (00-2) Cod -D6 51682 ND ng/ml

2016707760 821-1-A.A -.d OxCod OxCod-D3 13307 212409 0.0626 5.87 ng/ml

2016707760 821-1-A.A -.d HyCod HyCod-d3 135905 ND rig/ml

2016707760 821-1-A.A -.d 6 -AM (C0-2) 6 -AM -d3 109603 ND rig/ml


SB 2016026838 1.A -.d Morph (cam Mor-D6 ND rig/ml


SB 2016026838 1.A -.d HyMor HyMor-D3 ND rig/ml

SB 2016026838 1.A -.d Cod (C0-2) Cod -D6 ND rig/ml

SB 2016026838 1.A -.d OxCod OxCod-D3 ND rig/ml

SB 2016026838 1.A -.d HyCod HyCod-d3 ND rig/ml

SB 2016026838 1.A -.d 6 -AM (C0-2) 6 -AM -d3 ND rig/m1


2016026838 1.A -.d Morph (C0-10) Mor-D6 15054 38293 0.3931 30.04 ng/ml

2016026838 1.A -.d OxMor OxyMor-D3 125325 ND rig/ml

2016026838 1.A -.d HyMor HyMor-D3 525 99113 0.0053 0.89 rig/ml

2016026838 1.A -.d Cod (C0-2) Cod -D6 784 49862 0.0157 1.50 rig/ml

2016026838 1.A -.d OxCod OxCod-D3 726 210006 0.0035 0.32 rig/m1

2016026838 1.A -.d HyCod HyCod-d3 130820 ND rig/m1

2016026838 1.A -.d 6 -AM (C0-2) 6 -AM -d3 103459 ND rig/m1

Data File Compound ISTD Exp Conc Response ISM Resp Resp Ratio Accuracy Concentration

SB 2016706947 01.A -.d Morph (00-10) Mor-D6 ND ng/m1


SB 2016706947 01.A -.d HyMor HyMor-D3 ND ng/m1



SB 2016706947 01.A -.d HyCod HyCod-d3 ND rig/m1





2016706947 01.A -.d Morph (C0-10) Mor-D6 39731 ND ng/ml


2016706947 01.A -.d HyMor HyMor-D3 99599 ND ng/ml

2016706947 01.A -.d Cod (C0-2) Cod -D6 50379 ND ng/ml


2016706947 01.A -.d HyCod HyCod-d3 29932 133581 0.2241 19.98 ng/ml

2016706947 01.A -.d 6 -AM (00-2) 6 -AM -d3 106460 ND ng/ml


SB 2016707154 285 -LA -.d Morph (C0-10) Mor-D6 ND ng/ml

SB 2016707154 285-1.A -.d OxMor OxyMor-D3 ND ng/ml

SB 2016707154 285-1.A -.d HyMor HyMor-D3 ND ng/ml

SB 2016707154 285-1.A -.d Cod (C0-2) Cod -D6 ND ng/ml

SB 2016707154 285-1.A -.d OxCod OxCod-D3 ND ng/ml

SB 2016707154 285-1.A -.d HyCod HyCod-d3 ND ng/m1

SB 2016707154 285-1.A -.d 6 -AM (C0-2) 6 -AM -d3 ND ng/ml


2016707154 285-1.A -.d Morph (00-10) Mor-D6 40562 ND ng/ml

2016707154 285-1.A -.d OxMor OxyMor-D3 134946 ND ng/ml

2016707154 285-1.A -.d HyMor HyMor-D3 103130 ND ng/ml

2016707154 285-1.A -.d Cod (C0-2) Cod -D6 51299 ND ng/ml

2016707154 285-1.A -.d OxCod OxCod-D3 14158 221733 0.0639 5.99 VIA2016707154 285-1.A -.d HyCod HyCod-d3 14910 135933 0.1097 9.88 ng/ml

2016707154 285-1.A -.d 6 -AM (C0-2) 6 -AM -d3 106378 ND ng/ml

Data File Compound ISTD Exp Conc Response ISM Resp Resp Ratio Accuracy Concentration









2016707417 2.A -.d Morph (CO -W) Mor-D6 41228 ND ng/ml


2016707417 2.A -.d HyMor HyMor-D3 5213 103072 0.0506 5.98 ng/ml




2016707417 2.A -.d 6 -AM (C0-2) 6 -AM -d3 108442 ND ng/ml



Data FileSB 2016707418 1.A -.dSB 2016707418 LA -.d

SB 2016707418 1.A -.d

SB 2016707418 1.A -.d

SB 2016707418 LA -.d

SB 2016707418 1.A -.d

SB 2016707418 1.A -.d

Data File2016707418 1.A -.d

2016707418 1.A -.d

2016707418 1.A -.d

2016707418 1.A -.d

2016707418 1.A -.d

2016707418 1.A -.d

2016707418 1.A -.d

Data FileSB 2016707597 0 -170464 -LA

SB 2016707597 0170464 -LA

SB 2016707597 0170464 -LA

SB 2016707597 CH70464-1.A

SB 2016707597 0 -170464 -LA

SB 2016707597 0 -170464 -LA

SB 2016707597 0170464 -LA

Data File

2016707597 CH70464-1.A -.d

2016707597 CH70464-1.A -.d

2016707597 CH70464-1.A -.d

2016707597 CH70464-1.A -.d

2016707597 CH70464-1.A -.d

2016707597 CH70464-1.A -.d

2016707597 0-170464-1.A -.d

Data File

SB 2016707598 CH70848-1.A

SB 2016707598 0-170848-1.A

SB 2016707598 0170848 -LA

SB 2016707598 CH70848-1.A

SB 2016707598 CH70848-1.A

SB 2016707598 0 -170848 -LA

SB 2016707598 CH70848-1.A

Compound ISTD

Morph (CO -10) Mor-D6OxMor OxyMor-D3

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (00-2)

Compound

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod6 -AM (C0-2)

-.d

-.d

-.d

-.d

-.d

-.d

-.d

-.d

-.d

-.d

-.d

-.d

-.d

-.d

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d36 -AM -d3

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Exp Conc

Exp Conc

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Response ISTD Resp Resp Ratio Accuracy Concentration

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

Response ISTD Resp Resp Ratio

10028 36467 0.2750

124353

549 94580 0.0058

47243

991 202597 0.0049

125262

99873

Exp Conc

Exp Conc

Exp Conc


21.22 ng/ml

ND ng/ml

0.94 ng/ml

ND ng/ml

0.46 ng/ml

ND ng/ml

ND ng/ml


ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml


37182 ND ng/ml

2296 125298 0.0183 2.27 ng/ml

5167 95185 0.0543 6.39 ng/ml

48261 ND ng/ml

3232 202414 0.0160 1.50 ng/ml

277680 120157 2.3110 192.79 ng/ml

98968 ND ng/ml


Arizona Department of Public Safety Page 12 of 194

Concentration

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

•

Printed at: 6:19 AM on: 8/15/2016


Data File2016707598 C1170848 -1.A -.d

2016707598 CH70848-1.A -.d

2016707598 CH70848-1.A -.d

2016707598 0 -170848 -LA -.d

2016707598 CH70848-1.A -.d

2016707598 CH70848-1.A -.d

2016707598 0170848 -LA -.d

Data FileSB 2016707708 IA -.d

SB 2016707708 1.A -.d

SB 2016707708 LA -.d

SB 2016707708 1.A -.d

SB 2016707708 1.A -.d

SB 2016707708 1.A -.d

SB 2016707708 1.A -.d

Data File2016707708 1.A -.d

2016707708 1.A -.d

2016707708 1.A -.d

2016707708 1.A -.d

2016707708 1.A -.d

2016707708 1.A -.d

2016707708 1.A -.d

Data File

SB 2016707775 2.A -.d

SB 2016707775 2.A -.d

SB 2016707775 2.A -.d

SB 2016707775 2.A -.d

SB 2016707775 2.A -.d

SB 2016707775 2.A -.d

SB 2016707775 2.A -.d

Data File2016707775 2.A -.d

2016707775 2.A -.d

2016707775 2.A -.d

2016707775 2.A -.d

2016707775 2.A -.d

2016707775 2.A -.d

2016707775 2.A -.d

Compound

Morph (co -n)OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

ISTDMor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

thryMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Compound

Morph (00-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Exp Conc

Exp Conc

Exp Conc

Exp Conc

Exp Conc

Response3900

679

ISTD Resp

36706

119892

93166

46044

196041

123321

95733

Resp Ratio Accuracy Concentration

0.1062 8.57 ng/ml

ND ng/ml

ND ng/ml

ND ng/ml0.0035 0.32 ng/ml

ND ng/ml

ND rig/nil


ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml


23752 27391 0.8671

92574

72436

2475 35100 0.0705

633 144063 0.0044

90364

72151

Concentration

65.10 ng/ml

ND ng/ml

ND ng/ml

5.94 ng/ml

0.41 ng/ml

ND ng/ml

ND ng/ml


ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

Response ISM Resp Resp Ratio

40533

5117 131445 0.0389

9892 103557 0.0955

51258

27225 216869 0.1255

138449

108670


ND ng/ml

4.52 ng/ml

11.01 ng/ml

ND ng/ml

11.76 ng/ml

ND ng/ml

ND ng/ml




SB 2016708210 0007.A -.d Morph (CO -W) Mor-D6 ND ng/ml









2016708210 0007.A -.d OxMor OxyMor-D3 2445 131394 0.0186 2.31 ng/ml





2016708210 0007.A -.d 6 -AM (C0-2) 6 -AM -d3 108579 ND ng/ml


SB 2016708347 200-01.A -.d Morph (CO -10) Mor-D6 ND ng/ml



SB 2016708347 200-01.A -.d Cod (C0-2) Cod -D6 ND ng/m1


SB 2016708347 200-01.A -.d HyCod HyCod-d3 ND ng/ml

58 2016708347 200-01.A -.d 6 -AM (C0-2) 6 -AM -d3 ND ng/ml


2016708347 200-01.A -.d Morph (C0-10) Mor-D6 7402 16310 0.4539 34.56 ng/ml

2016708347 200-01.A -.d OxMor OxyMor-D3 379 59703 0.0064 0.97 ng/ml

2016708347 200-01.A -.d HyMor HyMor-D3 790 42661 0.0185 2.38 ng/ml


2016708347 200-01.A -.d OxCod OxCod-D3 6047 95544 0.0633 5.93 ng/ml

2016708347 200-01.A -.d HyCod HyCod-d3 66738 ND ng/ml

2016708347 200-01.A -.d 6 -AM (C0-2) 6 -AM -d3 50335 ND ng/ml


SB AZ1600006320 24.A -.d Morph (C0-10) Mor-D6 ND ng/ml

SB AZ1600006320 24.A -.d OxMor OxyMor-03 ND ng/ml

SB AZ1600006320 24.A -.d HyMor HyMor-D3 ND ng/ml

SB AZ1600006320 24.A -.d Cod (C0-2) Cod -D6 ND ng/ml

SB AZ1600006320 24.A -.d OxCod OxCod-D3 ND ng/ml

SB AZ1600006320 24.A -.d HyCod HyCod-d3 ND ng/ml

SB AZ1600006320 24.A -.d 6 -AM (C0-2) 6 -AM -d3 ND ng/ml


\f



AZ1600006320 24.A -.d Morph (C0-10) Mor-D6 12965 41242 0.3144 24.17 ng/ml

AZ1600006320 24.A -.d OxMor OxyMor-D3 129273 ND rig/aA21600006320 24.A -.d HyMor HyMor-D3 101873 ND ng/ml

AZ1600006320 24.A -.d Cod (C0-2) Cod -D6 1462 50314 0.0291 2.58 ng/ml

AZ1600006320 24.A -.d OxCod OxCod-D3 763 209712 0.0036 0.34 ng/ml

AZ1600006320 24.A -.d HyCod HyCod-d3 131574 ND ng/ml

AZ1600006320 24.A -.d 6 -AM (C0-2) 6 -AM -d3 107132 ND ng/ml


SB AZ1600006929 1.A -.d Morph (CO -10) Mor-D6 ND rig/aSB AZ1600006929 1.A -.d OxMor OxyMor-D3 ND ng/ml

SB AZ1600006929 1.A -.d HyMor HyMor-D3 ND ng/ml

SB AZ1600006929 1.A -.d Cod (C0-2) Cod -D6 ND ng/ml

SB AZ1600006929 1.A -.d OxCod OxCod-D3 ND ng/ml

SB AZ1600006929 1.A -.d HyCod HyCod-d3 ND ng/ml

SB AZ1600006929 1.A -.d 6 -AM (00-2) 6 -AM -d3 ND ng/ml


AZ1600006929 1.A -.d Morph (C0-10) Mor-D6 41662 ND ng/ml

AZ1600006929 1.A -.d OxMor OxyMor-D3 130307 ND rig/aAZ1600006929 1.A -.d HyMor HyMor-D3 3236 105619 0.0306 3.74 ng/ml

AZ1600006929 1.A -.d Cod (C0-2) Cod -D6 51256 ND ng/ml

AZ1600006929 1.A -.d OxCod OxCod-D3 705 217933 0.0032 0.30 ng/m1

AZ1600006929 1.A -.d HyCod HyCod-d3 104184 133127 0.7826 68.26 ng/ml

AZ1600006929 1.A -.d 6 -AM (C0-2) 6 -AM -d3 108746 ND ng/ml


SB 2016024724 1.A -.d Morph (C0-10) Mor-06 ND ng/ml


SB 2016024724 1.A -.d FlyMor HyMor-D3 ND ng/ml






2016024724 1.A -.d Morph (CO -W) Mor-06 39513 ND ng/ml






2016024724 1.A -.d 6 -AM (C0-2) 6 -AM -d3 102372 ND ng/ml


Data File Compound ISTD

SB before Medium Control.d Morph (CO -10) Mor-D6

SB before Medium Control.d OxMor OxyMor-D3

SB before Medium Control.d HyMor HyMor-D3

SB before Medium Control.d Cod (C0-2) Cod -D6

SB before Medium Controld OxCod OxCod-D3

SB before Medium Control.d HyCod HyCod-d3

SB before Medium Control.d 6 -AM (C0-2) 6 -AM -d3


Medium Control.d Morph (C0-10) Mor-D6

Medium Control.d OxMor OxyMor-D3

Medium Control.d HyMor HyMor-D3

Medium Control.d Cod (C0-2) Cod -D6

Medium Control.d OxCod OxCod-D3

Medium Contro1.d HyCod HyCod-d3

Medium Control.d 6 -AM (00-2) 6 -AM -d3


Exp Conc

Exp Conc Response ISTD Resp Resp Ratio Accuracy Concentration

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml


26073 41306 0.6312 47.72 ng/ml

60745 132931 0.4570 49.50 ng/ml

44135 105362 0.4189 46.83 ng/ml

32434 54030 0.6003 49.37 ng/ml

115224 222122 0.5187 48.31 ng/ml

76555 140538 0.5447 47.90 ng/ml

54361 108667 0.5003 47.81 ng/ml


SB 2016025509 I -1.A -.d Morph (CO -10) Mor-D6 ND ng/ml



SB 2016025509 1-1.A -.d Cod (C0-2) Cod -D6 ND ng/ml


SB 2016025509 1-1.A -.d HyCod HyCod-d3 ND ng/ml

SB 2016025509 14.A -.d 6 -AM (CO2) 6 -AM -d3 ND ng/ml


2016025509 1-1.A -.d Morph (C0-10) Mor-D6 1024 40569 0.0253 2.47 ng/ml

2016025509 1-1.A -.d OxMor OxyMor-D3 127025 ND ng/ml

.2016025509 1-1.A -.d HyMor HyMor-D3 101823 ND ng/ml


2016025509 1-1.A -.d OxCod OxCod-D3 2701 204523 0.0132 1.24 ng/ml

2016025509 1-1.A -.d HyCod HyCod-d3 130662 ND ng/ml

2016025509 1-1.A -.d 6 -AM (C0-2) 6 -AM -d3 101656 ND ng/ml





SB 2016026327 LA -.d Cod (C0-2) Cod -D6 ND ng/m1



SB 2016026327 IA -.d 6 -AM (00-2) 6 -AM -d3 ND ng/ml

Arizona Department of Public Safety Page 16 of 194 Printed at: 6:19 AM on: 8/15/2016 t\.t



2016026327 1.A -.d Morph (CO -10) Mor-D6 3451 38546 0.0895 7.31 ng/ml



2016026327 1.A -.d Cod (C0-2) Cod -D6 506 48096 0.0105 1.07 ng/ml



2016026327 1.A -.d 6 -AM (C0-2) 6 -AM -d3 99856 ND ng/ml


SB 2016026545 1.A -.d Morph (CO -W) Mor-D6 ND ng/ml





SB 2016026545 IA -.d FlyCod HyCod-d3 ND ng/ml



2016026545 1.A -.d Morph (CO -10) Mor-D6 42266 ND ng/ml






2016026545 1.A -.d 6 -AM (C0-2) 6 -AM -d3 114329 ND ng/ml


SB 2016026971 1SJ.A -.d Morph (C0-10) Mor-D6 ND ng/ml

SB 2016026971 1SJ.A -.d OxMor OxyMor-D3 ND ng/ml

SB 2016026971 1SJ.A -.d HyMor HyMor-D3 ND ng/ml

SB 2016026971 1SJ.A -.d Cod (C0-2) Cod -D6 ND ng/ml


SB 2016026971 1SJ.A -.d HyCod HyCod-d3 ND ng/ml

SB 2016026971 1SJ.A -.d 6 -AM (C0-2) 6 -AM -d3 ND ng/ml


2016026971 1SJ.A -.d Morph (C0-10) Mor-D6 39390 ND ng/ml

2016026971 1SJ.A -.d OxMor OxyMor-D3 3268 126142 0.0259 3.10 ng/ml

2016026971 1SJ.A -.d HyMor HyMor-D3 101928 ND ng/ml

2016026971 1SJ.A -.d Cod (C0-2) Cod -D6 523 50044 0.0104 1.07 ng/ml

2016026971 1SJ.A -.d OxCod OxCod-D3 504977 202803 2.4900 225.56 ng/ml

2016026971 1S1A -.d HyCod HyCod-d3 605 135321 0.0045 0.53 ng/ml

2016026971 153.A -.d 6 -AM (C0-2) 6 -AM -d3 102265 ND ng/ml



Data FileSB 2016708312 CH71670-1.A -.d

SB 2016708312 0171670-1.A -.d

SB 2016708312 0171670-1.A -.d

SB 2016708312 CH71670-1.A -.d

SB 2016708312 0171670-1.A -.d

SB 2016708312 CH71670-1.A -.d

SB 2016708312 0171670 -LA -.d

Data File

2016708312 CH71670-1.A -.d

2016708312 CH71670-1.A -.d

2016708312 CH71670-1.A -.d

2016708312 0171670-1.A -.d

2016708312 CH71670-1.A -.d

2016708312 CH71670-1.A -.d

2016708312 CH71670-1.A -.d

Data File

SB 2016708396 6RM.A -.d

SB 2016708396 6RM.A -.d

SB 2016708396 6RM.A -.d

SB 2016708396 6RM.A -.d

SB 2016708396 6RM.A -.d

SB 2016708396 6RM.A -.d

SB 2016708396 6RM.A -.d

Data File

2016708396 6RM.A -.d

2016708396 6RM.A -.d

2016708396 6RM.A -.d

2016708396 6RM.A -.d

2016708396 6RM.A -.d

2016708396 6RM.A -.d

2016708396 6RM.A -.d

Data File

SB 2016708450 1.A -.d

SB 2016708450 1.A -.d

SB 2016708450 1.A -.d

SB 2016708450 LA -.d

SB 2016708450 1.A -.d

SB 2016708450 1.A -.d

SB 2016708450 1.A -.d

Compound

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-06

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Exp Conc

Exp Conc

Exp Conc

Exp Conc

Exp Conc


ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

Response ISM Reap Resp Ratio Accuracy Concentration

42031 39713 1.0584 79.10 ng/ml

130478 ND ng/ml

355 105622 0.0034 0.67 ng/ml

5176 51334 0.1008 8.40 ng/ml

811 215221 0.0038 0.35 ng/ml

134619 ND ng/ml

106609 ND ng/ml

Response

Response

377

101043

2157

Response

ISM Resp Resp Ratio Accuracy Concentration

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml


43232 ND ng/ml

135409 0.0028 0.58 ng/ml

107467 ND ng/ml

53098 ND ng/ml

220345 0.4586 42.75 ng/ml

139158 0.0155 1.51 ng/ml

109444 ND ng/ml


ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml



Data File2016708450 1.A -.d

2016708450 1.A -.d

2016708450 1.A -.d

2016708450 1.A -.d

2016708450 1.A -.d

2016708450 1.A -.d

2016708450 1.A -.d

Data FileSB 2016708468 1.A -.d

SB 2016708468 1.A -.d

SB 2016708468 1.A -.d

SB 2016708468 1.A -.d

SB 2016708468 1.A -.d

SB 2016708468 LA -.d

SB 2016708468 1.A -.d

Data File

2016708468 1.A -.d

2016708468 1.A -.d

2016708468 1.A -.d

2016708468 1.A -.d

2016708468 LA -.d

2016708468 LA -.d

2016708468 1.A -.d

Data FileSB 2016708665 41603.A -.d

SB 2016708665 41603.A -.d

SB 2016708665 41603.A -.d

SB 2016708665 41603.A -.d

SB 2016708665 41603.A -.d

SB 2016708665 41603.A -.d

SB 2016708665 41603.A -.d

Data File2016708665 41603.A -.d

2016708665 41603.A -.d

2016708665 41603.A -.d

2016708665 41603.A -.d

2016708665 41603.A -.d

2016708665 41603.A -.d

2016708665 41603.A -.d

Compound

Morph (CO -W)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (CO -10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (W-2)

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

Compound

Morph (C0-10)

OxMor

HyMor

Cod (C0-2)

OxCod

HyCod

6 -AM (C0-2)

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

ISTD

Mor-D6

OxyMor-D3

HyMor-D3

Cod -D6

OxCod-D3

HyCod-d3

6 -AM -d3

Exp Conc

Exp Conc

Exp Conc


4684 45972 0.1019

3449 143056 0.0241

418 114715 0.0036

56128

153030 231094 0.6622

487 151228 0.0032

118956

Response ISM Resp Resp Ratio

Response

Exp Conc

Exp Conc

993

122430

9711

ISM Resp36524

133270 0.0074

106519

54300

228389 0.5361

146199 0.0664

102374

Concentration

8.24 ng/ml

2.91 ng/ml

0.70 ng/ml

ND ng/ml

61.55 ng/ml

0.42 ng/ml

ND ng/ml


ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml


ND ng/ml

1.09 ng/ml

ND ng/ml

ND ng/ml

49.91 ng/ml

6.05 ng/ml

ND ng/ml


Response ISM Resp46417

2106 147580 0.0143

117669

58641

58125 245485 0.2368

607 156228 0.0039

111698

Concentration

ND ng/ml

ND ng/m1

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml


ND ng/ml

1.83 ng/ml

ND ng/ml

ND ng/ml

22.14 ng/ml

0.48 ng/ml

ND ng/ml






SB 2016708744 LA -.d HyMor HyMor-D3 ND ng/ml

SB 2016708744 LA -.d Cod (C0-2) Cod -D6 ND ng/ml



SB 2016708744 1.A -.d 6 -AM (00-2) 6 -AM -d3 ND ng/ml

Data File Compound ISTD Exp Conc Response !STD Resp Resp Ratio Accuracy Concentration



2016708744 1.A -.d HyMor HyMor-D3 521 116693 0.0045 0.79 ng/ml

2016708744 1.A -.d Cod (C0-2) Cod -D6 12251 56944 0.2151 17.71 ng/m1



2016708744 1.A -.d 6 -AM (C0-2) 6 -AM -d3 1045 118942 0.0088 1.12 ng/ml




SB 2016709020 I.A -.d HyMor HyMor-D3 ND ng/ml






2016709020 1.A -.d Morph (CO -10) Mor-D6 41588 ND ng/ml






2016709020 1.A -.d 6 -AM (C0-2) 6 -AM -d3 108654 ND ng/ml


SB 2016709036 1001-1-A.A -.d Morph (C0-10) Mor-D6 ND ng/ml

SB 2016709036 1001-1-A.A -.d OxMor OxyMor-D3 ND ng/m1

SB 2016709036 1001-1-A.A -.d HyMor HyMor-D3 ND ng/ml

SB 2016709036 1001-1-A.A -.d Cod (C0-2) Cod -D6 ND ng/ml

SB 2016709036 1001-1-A.A -.d OxCod OxCod-D3 ND ng/ml

SB 2016709036 10014-A.A -.d HyCod HyCod-d3 ND ng/ml

SB 2016709036 1001-1-A.A -.d 6 -AM (C0-2) 6 -AM -d3 ND ng/ml




2016709036 1001-1-A.A -.d Morph (C0-10) Mor-D6

2016709036 1001-1-A.A -.d OxMor OxyMor-D3

2016709036 1001-1-A.A -.d HyMor HyMor-D3

2016709036 1001-1-A.A -.d Cod (C0-2) Cod -D6

2016709036 1001-1-A.A -.d OxCod OxCod-D3

2016709036 1001-1-A.A -.d HyCod HyCod-d3

2016709036 1001-1-A.A -.d 6 -AM (C0-2) 6 -AM -d3


SB AZ1600007447 A)1 -1.A -.d Morph (C0-10) Mor-D6

SB AZ1600007447 All -1.A -.d OxMor OxyMor-D3

SB AZ1600007447 All -LA -.d HyMor HyMor-D3

SB AZ1600007447 All -1.A -.d Cod (C0-2) Cod -D6

SB A21600007447 All -1.A -.d OxCod OxCod-D3

SB AZ1600007447 A)1 -1.A -.d HyCod HyCod-d3

SB A21600007447 A31 -1.A -.d 6 -AM (C0-2) 6 -AM -d3

Exp Conc

Exp Conc


44141 ND ng/ml

3547 134050 0.0265 3.16 ng/ml

107971 ND ng/ml

51455 ND ng/ml

169393 210203 0.8059 74.74 ng/ml

1225 136146 0.0090 0.93 ng/ml

110907 ND ng/ml


ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml


Q1600007447 All -1.A -.d Morph (CO -W) Mor-D6 7995 39208 0.2039 15.90 ng/ml

AZ1600007447 All -1.A -.d OxMor OxyMor-D3 123583 ND ng/ml

AZ1600007447 All -1.A -.d HyMor HyMor-D3 98332 ND ng/ml

Q1600007447 All -1.A -.d Cod (C0-2) Cod -D6 1049 46079 0.0228 2.07 ng/ml

AZ1600007447 A31 -1.A -.d OxCod OxCod-D3 611 192177 0.0032 0.30 ng/ml

AZ1600007447 All -1.A -.d HyCod HyCod-d3 119943 ND ng/ml

Q1600007447 A.11 -1.A -.d 6 -AM (C0-2) 6 -AM -d3 99399 ND ng/ml




SB 2016712064 100.A -.d FlyMor HyMor-D3 ND ng/ml






2016712064 100.A -.d Morph (00-10) Mor-D6 40065 ND ng/ml






2016712064 100.A -.d 6 -AM (C0-2) 6 -AM -d3 102489 ND ng/ml



SB before High Control.d Morph (C0-10) Mor-D6

SB before High Control.d OxMor OxyMor-D3

SB before High Control.d HyMor HyMor-D3

SB before High Control.d Cod (C0-2) Cod -D6

SB before High Control.d OxCod OxCod-D3

SB before High Control.d HyCod HyCod-d3

SB before High Control.d 6 -AM (00-2) 6 -AM -d3


Exp Conc Response ISTD Reap Reap Ratio Accuracy Concentration

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

Data File Compound ISTD Exp Conc Response ISTD Reap Reap Ratio Accuracy Concentration

High Control.d Morph (C0-10) Mor-D6 102346 40780 2.5097 182.83 ng/m1

High Control.d OxMor OxyMor-D3 225275 133268 1.6904 175.52 ng/ml

High Control.d HyMor HyMor-D3 187681 104821 1.7905 191.25 ng/ml

High Control.d Cod (C0-2) Cod -D6 131456 55814 2.3553 199.47 ng/ml

High Control.d OxCod OxCod-D3 477637 234320 2.0384 185.79 ng/ml

High Control.d HyCod HyCod-d3 325667 142941 2.2783 190.24 ng/ml

High Control.d 6 -AM (C0-2) 6 -AM -d3 223848 109504 2.0442 187.18 ng/ml


Final Solvent Blank.d Morph (C0-10) Mor-D6

Final Solvent Blank.d OxMor OxyMor-D3

Final Solvent Blank.d HyMor HyMor-D3

Final Solvent Blank.d Cod (C0-2) Cod -D6

Final Solvent Blank.d OxCod OxCod-D3

Final Solvent Blank.d HyCod HyCod-d3

Final Solvent Blank.d 6 -AM (C0-2) 6 -AM -d3

Exp Conc Response ISM Reap Reap Ratio Accuracy Concentration

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml

ND ng/ml


Quant Calibration Report (ISTD)

Batch Info

Batch Data PathAnalysis TimeReport TimeLast Calib Update

Calibration Info

Target Compound

D:\2016 Data \JM \OPIATES\081216\QuantResults\081216jm.batch.bin8/15/2016 6:12 AM •8/15/2016 6:13 AM8/15/2016 6:12 AM

6 -AM (C0-2)6 -AM (C0-2) - 5 Levels, 5 Levels Used, 5 Points, 5 Points Used, 0 QCs

1 R"2 = 0.99995106y = 2.970441E-006 • x ^ 2 + 0.010380 x -0.002817

2.76a. 2.5 Type:Quadratic, Origin:Ignore, Wei9ht:1/x

Cr 2.25-o

2 -

1.75 -cc

1.5 -

1.25-

0.75-0.5-

0.25-

0 -

-0.25-- I I26 40 668 61 6 0 120 140 160 180 2602 20 240 260

Concentration (ng/ml)

Calibration Cal Type Level Enabled081216\CAL 2.d Calibration 1 El081216\CAL 5.d Calibration 2 El081216\CAL 25.d Calibration 3 0081216\CAL 100.d Calibration 4 El081216\CAL 250.d Calibration 5 ElISTD Compound 6 -AM -d3

Response Exp Conc RF1946 2.00 0.4525550 5.00 0.497

28647 25.00 0.505116109 100.00 0.536278142 250.00 0.555

Calibration Cal Type Level Enabled Response Exp Conc RF081216\CAL 2.d Calibration 1 El 107738 50.00 2154.767081216\CAL 5.d Calibration 2 El 111619 50.00 2232.372081216\CAL 25.d Calibration 3 El 113376 50.00 2267.524081216\CAL 100.d Calibration 4 10 108255 50.00 2165.103081216\CAL 250.d Calibration 5 El 100222 50.00 2004.435Target Compound Cod (C0-2)



Cod (C0-2) - 5 Levels, 5 Levels Used, 5 Points, 5 Points Used, 0 QCsy = -2.626372E-006* x ^ 2 + 0.012345 * x - 0.002728

E rc--4 = U.UUWW0400a 2.75- Type:Quadratic, Origin:Ignore, Weight:1/x• 2.5-

Mo 2.25->

.1' 2 -

a ' 1.75-1.5-

1.25-

1-10.75-0.5-

0.25-o -

-0.25-26 40 66 80 1601 2 01 4 01 6 01 8 0 2602 2 0 240 260


Calibration081216\CAL 2.d081216\CAL 5.d081216\CAL 25.d081216\CAL 100.d081216\CAL 250.dISTD Compound Cod-D6Calibration081216\CAL 2.d081216\CAL 5.d081216\CAL 25.d081216\CAL 100.d081216\CAL 250.dTarget Compound HyCod

Cal Type Level EnabledCalibration 1Calibration 2Calibration 3Calibration 4 LJCalibration 5

Cal Type Level EnabledCalibration 1 l ICalibration 2 ElCalibration 3 ElCalibration 4 ElCalibration 5 El


16459 25.00 0.60264127 100.00 0.605

154630 250.00 0.584

Response Exp Conc RF51655 50.00 1033.10052766 50.00 1055.31554711 50.00 1094.21653012 50.00 1060.23552991 50.00 1059.821

HyCod -5 Levels, 5 Levels Used, 5 Points, 5 Points Used, 0 QCsy = 4.078862E-006 * x 2 + 0.011208* x -0.001486

• 3 R^2 = 0.999987112.75 Type:Quadratic, Origin:Ignore, Weight:1/x

D 2.5-? 2.25-- i°, 2 -cC 1.75-

1.5 -

1.25-1 -

0.75-0.5-

0.25-o-

-0.25-0I

26 46 6I0 86 160 120 140 160 180 260 220 240 260Concentration (ng/ml)

Calibration Cal Type Level Enabled081216\CAL 2.d Calibration 1 0081216\CAL 5.d Calibration 2 El081216\CAL 25.d Calibration 3 El081216\CAL 100.d Calibration 4 El081216\CAL 250.d Calibration 5 0

ISTD Compound HyCod-d3


39306 25.00 0.559159784 100.00 0.581396558 250.00 0.611



Calibration Cal Type Level Enabled Response Exp Conc RF081216\CAL 2.d Calibration 1 El 131943 50.00 2638.866081216\CAL 5.d Calibration 2 El 136189 50.00 2723.788081216\CAL 25.d Calibration 3 El 140671 50.00 2813.420081216\CAL 100.d Calibration 4 El 137589 50.00 2751.780081216\CAL 250.d Calibration 5 0 129798 50.00 2595.964


Q.,Quant Calibration Report (ISTD)

Target Compound HyMor

HyMor - 5 Levels, 5 Levels Used, 5 Points, 5 Points Used, 0 QCsy = 2.595669E-006 * x 2 + 0.008879 * x - 0.002581

2.4- RA2 = 0.999904418. 2.2- Type:Quadratic, Origin:Ignore, Weight:1/x

2 -CsCs

1rxco 8 -> •

1.6 -

et 1.4-1.2 -

1 -

0.8-0.6-0.4 -

0.2-o-

-0.2-20 40 608 01 0 0 1201 40 1601 802 002 20 240 260


Calibration Cal Type Level Enabled Response Exp Conc RF081216\CAL 2.d Calibration 1 0 1515 2.00 0.383081216\CAL 5.d Calibration 2 0 4280 5.00 0.425081216\CAL 25.d Calibration 3 0 22163 25.00 0.428081216\CAL 100.d Calibration 4 0 91692 100.00 0.460081216\CAL 250.d Calibration 5 0 223216 250.00 0.475ISTD Compound HyMor-D3Calibration Cal Type Level Enabled Response Exp Conc RF081216\CAL 2.d Calibration 1 0 98772 50.00 1975.444081216\CAL 5.d Calibration 2 El 100679 50.00 2013.578081216\CAL 25.d Calibration 3 0 103563 50.00 2071.254081216\CAL 100.d Calibration 4 El 99607 50.00 1992.132081216\CAL 250.d Calibration 5 0 93929 50.00 1878.588ISTD Compound Mor-06Calibration081216\CAL 2.d081216\CAL 5.d081216\CAL 25.d081216\CAL 100.d081216\CAL 250.dTarget Compound

Cal Type Level Enabled Response Exp Conc RFCalibration 1 0 38741 50.00 774.811Calibration 2 El 41701 50.00 834.020Calibration 3 El 41780 50.00 835.609Calibration 4 El 39464 50.00 789.287Calibration 5 0 35433 50.00 708.661

Morph (00-10)Morph (C0-10) - 5 Levels, 5 Levels Used, 5 Points, 5 Points Used, 0 QCs

u) 35.g •

g 3 -rt2.5-

Cs

1 -I

0.5-1

0 -I

y = 2.827637E-006 *x "2 + 0.013251 • x - 0.007519= 0.99992728

Type:Quadratic, Origin:Ignore, Weight: 1/x

20 60 100 120 140 160 160 200 220 240 260Concentration (ng/ml)



Calibration Cal Type Level Enabled Response Exp Conc RF081216\CAL 2.d Calibration 1 762 2.00 0.492



Calibration081216\CAL 5.d081216\CAL 25.d081216\CAL 100.d081216\CAL 250.dTarget Compound OxCod

Cal Type Level EnabledCalibration 2Calibration 3Calibration 4Calibration 5

Response Exp Conc RF2430 5.00 0.583

13244 25.00 0.63453610 100.00 0.679

123230 250.00 0.696

OxCod - 5 Levels, 5 Levels Used, 5 Points, 5 Points Used, 0 QCsy = 1.707855E-006 • x "2 + 0.010654 • x + 2.502385E-005

2 2.75 H Fr2 = 0.99990599Type:Quadratic, Origin:Ignore, Weight:1/x

cC 2.25-a> 2 -a 1.75-

1.5-1.25-

1 -

0.75-0.5-

0.25-0-

-0.25-26 46 66 86 160 120 140 160 180 260 220 240 260


Calibration081216\CAL 2.d081216\CAL 5.d081216\CAL 25.d081216\CAL 100.d081216\CAL 250.dISTD Compound OxCod-D3Calibration081216\CAL 2.d081216\CAL 5.d081216\CAL 25.d081216\CAL 100.d081216\CAL 250.dTarget Compound OxMor

Cal Type Level EnabledCalibration 1Calibration 2Calibration 3Calibration 4Calibration 5

Cal Type Level EnabledCalibration 1 IZI

Calibration 2Calibration 3Calibration 4Calibration 5 El

Response Exp Conc RF4559 2.00 0.540

11844 5.00 0.53858041 25.00 0.519

234101 100.00 0.547600619 250.00 0.553

Response Exp Conc RF210933 50.00 4218.658220152 50.00 4403.038223818 50.00 4476.356214092 50.00 4281.832217092 50.00 4341.832

OxMor -5 Levels, 5 Levels Used, 5 Points, 5 Points Used, 0 QCs2.6H y = 2.875935E-006 • x" 2 + 0.009140* x - 0.002476

11^2 = 0 99989062 . _ .type:Quadratic, ungin:ignore, weight:1/xp, 2.2-

cc 2 -

>

• 1.6-CC 1.4 -

1.2 -

1 -0.8-0.6-0.4 -

0.2 -

0-1-0.2

26 46 60 86 160 120 140 160 180 260 220 24.0 260Concentration (ng/ml)

Calibration Cal Type Level Enabled Response Exp Conc RF



081216\CAL 2.d Calibration 1 El 2035 2.00 0.412081216\CAL 5.d Calibration 2 El 5522 5.00 0.425081216\CAL 25.d Calibration 3 El 28909 25.00 0.441



Calibration Cal Type Level Enabled Response Exp Conc RF081216\CAL 100.d Calibration 4 WI 118520 100.00 0.476081216\CAL 250.d Calibration 5 EZI 294573 250.00 0.492ISTD Compound OxyMor-03Calibration Cal Type Level , Enabled Response Exp Conc RF081216\CAL 2.d Calibration 1 EZI 123373 50.00 2467.459081216\CAL 5.d Calibration 2 El 129776 50.00 2595.521081216\CAL 25.d Calibration 3 El 131006 50.00 2620.123081216\CAL 100.d Calibration 4 El 124617 50.00 2492.345081216\CAL 250.d Calibration 5 lil 119812 50.00 2396.243


SB before Batch 1

Batch Data Path D: \ 2016 DataVM \OPIATES \ 081216 \QuantResults \ 081216jm.batch.bin

Sample Position Vial 1 Sample Name SB before Batch 1

Instrument 6460-6 CommentAcquisition Method Blood Opiates.m Acquisition Time 8/12/2016 10:47 AM

Operator JM

+ TIC MRM (**->**) SB before Batch 1.dx10 2 _1

z

8-1

7 -

6 -

5

4 -

3 -

2 -

1 -

0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 4.2 4.4 4.6 4.8 5 5.2 5.4 5.6 5.8Acquisition Time (min)

Compound RT RI Range Transition Area Q1 Q1 Range ng/mLMor-D6 (1.36-1.51) 292.2 -> 152.1

Morph (C0-10) (1.38-1.52) 286.2 -> 152.1 nd

OxyMor-D3 (1.72-1.91) 305.2 -> 287.1OxMor (1.74-1.92) 302.2 -> 284.0 nd

HyMor-D3 (2.20-2.43) 289.2 -> 185.0HyMor (2.21-2.44) 286.2 -> 185.0

Cod -D6 (3.34-3.69) 306.2 -> 115.0Cod (C0-2) (3.41-3.77) 300.2 -> 115.0OxCod-D3 (3.97-4.39) 319.2-> 301.1

OxCod (4.04-4.46) 316.2 -> 298.2HyCod-d3 (4.54-5.02) 303.2 -> 199.0

HyCod (4.56-5.04) 300.2 -> 199.0

6 -AM -d3 (4.82-5.33) 331.2 -> 165.06 -AM (C0-2) (4.82-5.33) 328.2 -> 165.0

Mor-D6

292.2-' 152.1 , 292.2.> 128.0ti x10 1.1ourAot,30

C.)

4 -

3 -

2 -

1 -

1 1.2 1.4 1.6 1.8Acquisition Time (min)

Morph (C0-10) OxyMor-D3

286.2-' 152.1 , 286.2 -> 128.0

x10 1] Not Foundz

(-) 4

3

2

1

0


305.2 -> 287.1 , 305.2-> 230.1

30

x10 1-1 Not Found

876

54

3

21

1.4 1.6 1.8 2 2.2Acquisition Time (min)

nd

nd

nd

nd

(40.0-60.0) nd

OxMor

302.2-' 284.0 , 302.2 -> 227.1IA x10 1 Not F und

o 60 _ A

5

4

3

2

-



E XIV - Not round E Not rouna NO! I-0000

I

4 -

3-

2 -

1 -

0 -

2 2.2 2.4 2.6Acquisition Time (min)

OxCod-D3

319.2 ->301.1 , 319.2-> 244.0

x10 2-ti ' •O 1 4 -

1.2-

1 -

0.8-

0.6

0.4

0.2

0

Not Found

3.8 4 4.2 4.4 4.6Acquisition Time (min)

6 -AM -d3

331.2-> 165.0 , 331.2-> 152.0

i2 x10 2] Not Found

0.8-0.7-0.6-n g.0.4-0.3-0.2-

0.1-0 -1

4.8 5 5.2 5.4

Acquisition Time (min)

0 4 -

3 -

2 -

1 -

0 -


OxCod

E XIV "

5 -

4 -

3 -

2 -

1 -

0 -

3.2 3.4 3.6 3.8Acquisition Time (min)

HyCod-d3

316.2 -> 298.2 , 316.2 -> 241.0

.2 x102. j .Not Found

1

0.8

0.6

0.4

0.2

0


6 -AM (C0-2)

328.2-> 165.0 , 328.2-> 211.0

x10 11 Not Found

O • 6-0

5 -

4 -

3 -

2 -

1 -

0 -

I I4!8

Acquisition Time (mm)

303.2-> 199.0 , 303.2 ->171.0

x10 1_ Not Found

6 -

5

4 -

3 -

2 -

1 -

0 -

5'1 5I.2Acquisition Time (min)

E XIV I NOt 1-0U00b' 6-1

5-15-1

4-1

3-1

2-1

1-

0-


HyCod

300.2-> 199.0 , 300.2 -> 128.0

510 1 Not Found

• 4

2-1

1-

0 -1

4.6 4.7 4.8 4.9 5 5.1 5.2Acquisition Time (min)


SB before Batch 1

HyMor-D3 HyMor Cod -D6 Cod (C0-2)

289.2-> 185.0 , 289.2 -> 157.0 I 286.2-> 185.0, 286.2-> 157.0 306.2 -> 115.0 , 306.2 ->152.0 I 300.2 -> 115.0 , 300.2 ->152.0

SB before Batch 2

Batch Data Path

Sample Position

InstrumentAcquisition Method

Operator

D:\2016 Data \WI \OPIATES \081216 \QuantResults\081216jm.batch.bin

Vial 1

6460-6

Blood Opiates.m

JM

Sample Name SB before Batch 2Comment

Acquisition Time 8/12/2016 11:01 AM

+ TIC MRM (** -> **) SB before Batch 2.d

f l x10 2j5

6.5-6 -

5.5-5 -

4.5-4 -

3.5-3 -

2.5-2 -

1.5-1 -

0.5-

I i l

LJNI

0:6 0:8 11 1:2 1:4 1:6 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4 4:2 4:4 4:6 4:8I

5:2 5.4 5:6 5!8Acquisition Time (min)

Compound RT RT Range Transition Area Q1 Q1 Range ng/mLMor-D6 (1.36-1.51) 292.2 -> 152.1

Morph (C0-10) (1.38-1.52) 286.2 -> 152.1 nd

OxyMor-D3 (1.72-1.91) 305.2 -> 287.1OxMor (1.74-1.92) 302.2 -> 284.0 nd

HyMor-D3 (2.20-2.43) 289.2 -> 185.0HyMor (2.21-2.44) 286.2 -> 185.0 nd V

Cod -D6 (3.34-3.69) 306.2 -> 115.0Cod (C0-2) (3.41-3.77) 300.2 -> 115.0 nd

OxCod-D3 (3.97-4.39) 319.2 -> 301.1OxCod (4.04-4.46) 316.2 -> 298.2 nd

HyCod-d3 (4.54-5.02) 303.2 -> 199.0HyCod (4.56-5.04) 300.2 -> 199.0

6 -AM -d3 (4.82-5.33) 331.2 -> 165.06 -AM (C0-2) (4.82-5.33) 328.2 -> 165.0

Mor-D6

292.2 -> 152.1 , 292.2 -> 128.0

x10 1] ot Foui

U 4

3-1

2-1

1-1

0-1

1 1.2 1.4 1.6 1.8Acquisition Time (mm)


286.2-' 152.1 , 286.2-' 128.0x10 l NotFound

8 4

3-

2-

1 -

0 -

1 11.2 1.I4 1I.6 1Acquisition Time (mm)

305.2 -> 287.1 , 305.2-> 230.1

1.4 1.6 1.8 2 2.2Acquisition Time (mm)

nd

nd

OxMor

T I T1.4 1.6 1.8 2 2.2



SB before Batch 2

HyMor-D3

289.2 ->185.0 , 289.2 -> 157.0

.g x10 1-1 Not Found

U 5

2 2I.2 2:4 21.6Acquisition Time (min)

OxCod-D3

319.2 ->301.1 , 319.2 -> 244.0

x10 2

0.80.7

0.6-

0.5-0.4-

0.3-0.2-

0.1-0 -

Not Found

3:8 4I 4.2Acquisition Time (min)

6 -AM -d3

331.2 ->165.0 , 331.2-> 152.0

tl 1(101 1 Not Found

3 5

4

3-1

2

0

4.8 5 5.2 5.4Acquisition Time (min)

FlyMor Cod -D6

286.2-> 185.0 , 286.2 -> 157.0

x10 1- Not Found

4 -

3.5 -3 -

2.5 -

2 -1.5 -

1 -0.5 -

0

2 2I.2 2.4 2:6Acquisition Time (min)

OxCod

306.2 ->115.0 , 306.2 -> 152.0

8

x10 11 Not Found

3 -

2 -

1-

o-

3.I6 3:8Acquisition Time (mm)

HyCod-d3

316.2 -> 298.2 , 316.2 -> 241.0

x10 2

00 0.8

0.6

0.4

0.2

0

3!8 4I.4 41.6Acquisition Time (min)

6 -AM (C0-2)

328.2-> 165.0 , 328.2 -> 211.0

x10 1-1 Not Found3o

°

5

4

3

2

1

0


303.2-> 199.0 , 303.2 -> 171.0

4Cz0

x10 1

5

4

3

2

1

0

Not Found

- 1- 1 T 1 1 1 14.5 4.6 4.7 4.8 4.9 5 5.1 5.2


Cod (C0-2)

300.2 -> 115.0 , 300.2 -> 152.0

x10 11 Not Found

0 4

3-1

1-1

0-1

I I I -r3.2 3.4 3.6 3.8 4


HyCod

300.2 -> 199.0 , 300.2-> 128.0

.g x10 1 Not Found

4

3-1

2-1

1-

o-

4'.6 5'1 5 I.2Acquisition Time (min)


SB before Batch 35

Batch Data Path D:\2016 DataVM \OPIATES \ 081216 \QuantResults \081216jm.batch.bin

Sample Position Vial 1 Sample Name

Instrument 6460-6 Comment

Acquisition Method Blood Opiates.m Acquisition limeOperator

SB before Batch 3

8/12/2016 11:15 AM

+ TIC MRM (

I x10 2_

8 7 -

6 -

5 -

4 -

3 -

2 -

->

1.5-1 -

0.5-

*) SB before Batch 3.d

I .

0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 4.2 4.4 4.6 4.8 5 5.2 5.4 5.6 5.8Acquisition Time (min)


Morph (C0-10) (1.38-1.52) 286.2 -> 152.1 nd

OxyMor-D3 (1.72-1.91) 305.2 -> 287.1OxMor (1.74-1.92) 302.2 -> 284.0 nd

HyMor-D3 (2.20-2.43) 289.2 -> 185.0HyMor (2.21-2.44) 286.2 -> 185.0

Cod -D6 (3.34-3.69) 306.2 -> 115.0Cod (C0-2) (3.41-3.77) 300.2 -> 115.0

OxCod-D3 (3.97-4.39) 319.2 -> 301.1OxCod (4.04-4.46) 316.2 -> 298.2

HyCod-d3 (4.54-5.02) 303.2 -> 199.0HyCod (4.56-5.04) 300.2 -> 199.0

6 -AM -d3 (4.82-5.33) 331.2 -> 165.06 -AM (C0-2) (4.82-5.33) 328.2 -> 165.0

Mor-D6

292.2-> 152.1 , 292.2 -> 128.0

(-) 510 1]30

4

3-

2-

1 -

0 -


Morph (C0-10)

286.2-> 152.1 , 286.2 -> 128.0

.E x10 1 Not Found3 ".•"=>.-1:14 1(-) 4

2-

1 -

0 -

1 11.2 1:4 1.I6 1:8


OxyMor-D3

305.2 -> 287.1 , 305.2 ->230.1

x10

6 6-1t.)

451

3

2

1

Not Found


nd

nd

nd

nd

nd

OxMor

302.2-> 284.0 , 302.2 ->227.1



SB before Batch 3

HyMor-D3

289.2-> 185.0 , 289.2-> 157.0

ig x10 1_1 Not Found30

5

4-1

I I2 2.2 2.4 2.6


OxCod-D3

319.2-> 301.1 , 319.2 -> 244.0

x10 1-1 Not Found

A 8-1L I

7

651

5-1

4-1

3-11

2-1-

0 -

3I.8 4, 4:2 4:4 4:6Acquisition Time (min)

6 -AM -d3

331.2 ->165.0 , 331.2-> 152.0

xio11 Not Found3 5-1

41

3-

2-

1-1

4!13 5 5:2 5:4Acquisition Time (min)

HyMor Cod -D6

286.2-> 185.0 , 286.2 -> 157.0

•ig x10 1- N tF do

4 -

3.5 -3 -

2 -1.5 -

1 -0.5 -

0

2:2 2I.4 2:6Acquisition Time (min)

OxCod

316.2 ->298.2 , 316.2 ->241.0

x10 11 Not Foun8_7 _

6 -

5 -

4

3 -

2 -

1 -

0

3:8 4 4:2 4:4 4:6Acquisition Time (min)

6 -AM (C0-2)

306.2 -> 115.0 , 306.2 -> 152.0

,g x10 1 i NiitF

U 4

3

2-

I32 3:4 3:6 3:8


HyCod-d3

328.2-> 165.0 , 328.2 -> 211.0

4g xi°1

4 -

3 -

2 -

1 -

0 -

Found


303.2-> 199.0 , 303.2 -> 171.0

(-)

.g x10 I Not Found

4 -

3 -

2 -

1 -

0 -

I 1 1 1 1 - 1- I T4.5 4.6 4.7 4.8 4.9 5 5.1 5.2


Cod (C0-2)

r x.210->i - > 152.0

04

3-1

2-1

1-1

0-1

3:2 3:4 3:6 31=8 4Acquisition Time (min)

HyCod

300.2 ->199.0 , 300.2 -> 128.0

x10 11 Not Fou

(-) 4

I 4:6 4:7 4:8 4:9 5 5:1 5:2Acquisition Time (min)

651


CAL 2

Batch Data Path D:\2016 Data \.1M \OPIATES \081216 \QuantResults\081216jm.batch.bin

Sample Position P1 -Al Sample Name CAL 2


Acquisition Method Blood Opiates.m Acquisition Time 8/12/2016 11:29 AM

Operator JM

+ TIC MRM

xi° 4_10

3.5-

3 -

2.5-

2 -

1.5-

1 -

0.5-

* -> CAL 2.d

t oJ

0:6 0:8 21 1:2 1:4 1:6 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4 4:2 4:4 4:6 4:8 4 5:2 5:4 5:6 5:8Acquisition Time (min)

Compound RT RT Range Transition Area Q1

Mor-D6 1.43 (1.36-1.51) 292.2 -> 152.1 38741 60.7

Morph (C0-10) 1.44 (1.38-1.52) 286.2 -> 152.1 762 70.5

OxyMor-D3 1.79 (1.72-1.91) 305.2-> 287.1 123373 36.2

OxMor 1.81 (1.74-1.92) 302.2-> 284.0 2035 36.4

HyMor-D3 2.30 (2.20-2.43) 289.2 -> 185.0 98772 70.6

HyMor 2.31 (2.21-2.44) 286.2 -> 185.0 1515 66.3

Cod -D6 3.48 (3.34-3.69) 306.2 -> 115.0 51655 118.4

Cod (C0-2) 3.56 (3.41-3.77) 300.2 -> 115.0 1131 113.4

OxCod-D3 4.14 (3.97-4.39) 319.2 -> 301.1 210933 20.4

OxCod 4.20 (4.04-4.46) 316.2 -> 298.2 4559 23.3

HyCod-d3 4.76 (4.54-5.02) 303.2 -> 199.0 131943 40.8

HyCod 4.77 (4.56-5.04) 300.2 -> 199.0 2706 47.5

6 -AM -d3 5.06 (4.82-5.33) 331.2 -> 165.0 107738 46.1

6 -AM (C0-2) 5.06 (4.82-5.33) 328.2 -> 165.0 1946 55.5

Mor-D6 Morph (C0-10) OxyMor-D3

292.2-> 152.1 , 292.2-> 128.0

x10 41 Ratio = 60.7 (41.2 -72.3)

0.8-1

0.6-

0.4-

0

0.2-1

_

1.2 1.4 1.6 1.8Acquisition Time (mm)

286.2-> 152.1 , 286.2-> 128.0

xio 2-1 Ratio = 70.5 (5Q.8 - 76.2)3 2.25

2

1.75

1.5

1.25

1

0.75

0.5

0.25I1.2 11.4 1.I6 1:8


305.2 ->287.1 , 305.2-> 230.1

Ig x10 4] Ratio = 36.2 (2p.1 -43.7)

t§2 -

1.5-1

1-1

0.5-1

I1.4 1:6 1:8 2 2.2Acquisition Time (mm)

Q1 Range ng/mL(48.2-72.3)(50.8-76.2) 2.05

(29.1-43.7)(28.1-42.2) 2.07

(58.6-88.0)(53.9-80.8) 2.02

(95.1-142.6)(85.5-128.2) 2.00

(16.5-24.8)(16.2-24.3) 2.03

(32.0-48.1)

(35.9-53.9) 1.96

(36.4-54.6)(40.0-60.0) 2.01

OxMor

302.2-> 284.0 , 302.2 -> 227.1

ig x10 2- Ratio = 36.4 (2 .1 -42.2)3 4.5-0 4 -

3.5-3 -

2.5-

2 -1.5-

1 -0.5-

0 -I1.4 1:6 1:8 2 I2.2



CAL 2

HyMor-D3

289.2-> 185.0 , 289.2 ->157.0

4g x10 4-1 Ratio = 70.6 (4.6 -88.0)

0.5-1

2 2:2 2:4 2:6 rAcquisition Time (min)

OxCod-D3

319.2 ->301.1 , 319.2 -> 244.0

x10 4-1 Ratio = 20.4(16,5 -24.8)

o • 3.5-

3 -

2.5 -

2 -

1.51

1 -

0.5 -

0 -

3!8 44 :2 4:4 4:6Acquisition Time (min)

6 -AM -d3

331.2-> 165.0 , 331.2 -> 152.0

4gX10 4-I Ratio = 46.1 (44 - 54.6)

2.5-

2

1.5

1

0.5

0

4:8 5 5:2 5.4Acquisition Time (min)

HyMor Cod -D6

286.2-> 185.0 , 286.2 -> 157.0

x10 21 Ratio = 66.3 (4.9 -80.8)g 4 1

3.5 -

3 -

2.5 -

2 -

1.5-1

0.151

2 2:2 2:4 2:6Acquisition Time (min)

OxCod

306.2-> 115.0 , 306.2 -> 152.0

xlO

1.4-

1.2 -

1 -

0.8 -

0.6 -

0.4 -

0.2 -

-

3:2 3:4 3:6 3:8Acquisition Time (min)

HyCod-d3

316.2 ->298.2 , 316.2-> 241.0

x10 21 Ratio = 23.3 (16 2 - 24.3)

3 8 A7 -

6 -

5 -

4 -

3--


6 -AM (C0-2)

328.2-> 165.0 .328.2-> 211.0

.g x10 2- Ratio = 55.5 (4 .0 - 60.0)

0 5 -

4-1

3-1

2-1

1-1

0


303.2 ->199.0 , 303.2 -> 171.0

•CAc x10 4-I Ratio = 40.8(32.0 -48.1)

2.5-1

2-1

1.5-

1 -

0.5-

0 -

4.5 4.6 4.7 4.8 4.9 5 5.1 5.2Acquisition Time (min)

Cod (C0-2)

300.2 -> 115.0 , 300.2 -> 152.048 x10 21.Ratio.R..1.134.485.5,..12&2)..._

83-

2.5-

2 -

1.5-

1 -

0.5-

3:2 3:4 3:6 3:8 4Acquisition Time (min)

HyCod

300.2 -> 199.0 , 300.2 -> 128.0

x10 21 Ratio= 471 (35.9 - 53.9)

if? 6-1I-)

1 1 [ 1 1 [ 1 .4.6 4.7 4.8 4.9 5 5.1 5.2



CAL 5

Batch Data Path D: \ 2016 Data \JM \OPIATES \ 081216 \QuantResults \081216jm.batch.bin

Sample Position P1 -A2 Sample Name CAL 5

Instrument 6460-6 CommentAcquisition Method Blood Opiates.m Acquisition Time 8/12/2016 11:42 AM

Operator JM

+ TIC MRM

xio 41z0

4 -

3.5 -

3 -

2.5 -

2 -

1.5 -

1 -

0.5-1

-> *1 CAL 5.d

0 x o \

-o 4

I v

1-0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 4:2 4:4 4:6 4.8 5 5.2 5r.4 5.6 5.8Acquisition Time (min)


Mor-D6 1.43 (1.36-1.51) 292.2-> 152.1 41701 59.1

Morph (CO -W) 1.45 (1.38-1.52) 286.2 -> 152.1 2430 67.7

OxyMor-D3 1.81 (1.72-1.91) 305.2-> 287.1 129776 35.6

OxMor 1.82 (1.74-1.92) 302.2 -> 284.0 5522 34.1

HyMor-D3 2.30 (2.20-2.43) 289.2 -> 185.0 100679 71.2

HyMor 2.32 (2.21-2.44) 286.2 -> 185.0 4280 67.8

Cod -D6 3.49 (3.34-3.69) 306.2 -> 115.0 52766 119.9

Cod (C0-2) 3.57 (3.41-3.77) 300.2 -> 115.0 3144 108.6

OxCod-D3 4.16 (3.97-4.39) 319.2 -> 301.1 220152 20.4

OxCod 4.22 (4.04-4.46) 316.2 -> 298.2 11844 21.2

HyCod-d3 4.76 (4.54-5.02) 303.2 -> 199.0 136189 40.2

HyCod 4.78 (4.56-5.04) 300.2 -> 199.0 7637 43.8

6 -AM -d3 5.06 (4.82-5.33) 331.2 -> 165.0 111619 46.0

6 -AM (C0-2) 5.07 (4.82-5.33) 328.2 -> 165.0 5550 49.5

Mor-D6 Morph (C0-10)

292.2.> 152.1 , 292.2 -> 128.0

x10 4 I Ratio = 59.1 (44.2 -72.3)30

0.8

0.6

0.4

0.2


OxyMor-D3

286.2-> 152.1 , 286.2-> 128.0

.g x10 2-I Ratio = 67.7 (50,.8 - 76.2)

° 6-1

5-1

4

3

2-

1-

1:2 11.4 11.6 1:8Acquisition Time (min)

Q1 Range ng/mL(48.2-72.3)

(50.8-76.2) 4.96

(29.1-43.7)(28.1-42.2) 4.92

(58.6-88.0)(53.9-80.8) 5.07

(95.1-142.6)(85.5-128.2) 5.05

(16.5-24.8)

(16.2-24.3) 5.04

(32.0-48.1)

(35.9-53.9) 5.13

(36.4-54.6)(40.0-60.0) 5.05

305.2 -> 287.1 , 305.2.> 230.1

ig x10 4] Ratio = 35.6 (2)1 -43.7)30

2

1.5

0.5

I I-1.4 1.6 1.8 2 2.2


OxMor

302.2.> 284.0 , 302.2-> 227.1

t l x10 3 Ratio = 34.1 (2t1.1 -42.2)

1

0.8

0.6

0.4

0.2

I I I I



CAL 5

HyMor-D3

289.2.> 185.0 , 289.2-> 157.0

)2 x10 - Ratio = 71.2 (54.6 - 88.0)

0(3 21

1.5-

1 -

0.5-

0-

2!2 2.14 21.6Acquisition Time (min)

OxCod-D3

319.2 -> 301.1 , 319.2 -> 244.0

x10 4-1 Ratio = 20.4 (16.p -24.8)

3.5-1

3-

2.5-

2 -

1.5-

13.8 :4 41.2 41 1.4 4.6Acquisition Time (min)

6 -AM -d3

331.2 -> 165.0 , 331.2 -> 152.0

x10 41 Ratio = 46.0 (3§.4 - 54.6)

02.5-

2 -

1.5-

1 -

0.5-

0

1 14.8 55 .1 12 5.4


HyMor

286.2 ->185.0 , 286.2 -> 157.0

x10 3 I Ratio = 67.8 (519 -80.8)3 10

0.8

0.6

0.4

0.2

0


Cod -D6

OxCod

316.2 ->298.2 , 316.2-> 241.0

.g x10 3..] Ratio = 21.2 (16. - 24.3)

O 2

1.75-

1.5-

1.25-1 -

0.75-

0.5-0.25-

0-1

3 41.8 4 1 1.2 4!4 4.6Acquisition Time (min)

6 -AM (C0-2)

306.2-> 115.0 , 306.2-> 152.0

)2 x10 4 Ratio_5_119.9495.1.,1 _

O • 1.4 -

1.2 -

-

0.8 -

0.4 -

0.2 -

0 -

1.2 31 1.4 31.6 3.3 8Acquisition Time (min)

HyCod-d3

328.2 ->165.0 , 328.2 ->211.0

x10 3- Ratio = 49.5 (41.0 - 60.0)

O 1.4-

1.2-

1 -

0.8-

0.6-

0.4-

0.2-

0-1

1 1 18 5 5.2 54. .4Acquisition Time (min)

303.2-> 199.0 , 303.2 -> 171.0

xio4j Ratio = 40.2,(32.0 - 48.1)

(-) 2.5

2

1.5

1

0.5

15 1.6 4!7 41 5 1.8 4!9 54. 4 .1 51.2Acquisition Time (min)

Cod (C0-2)

300.2-> 115.0 , 300.2 -> 152.0

,g x10 21. Ratio_=.108.6.485.5.,128.2)_30 8

7-

6 -

5 -

4 -

3 -

2 -

1 -

0-3!2 31.4 31.6 3!8 4


HyCod

300.2 -> 199.0 , 300.2-> 128.0

x10 3-I Ratio = 43.8,(35.9 - 53.9)

3 1.61.4-1

1.2-11

1 .1

0.8

0.6-

0.4-

0.2-

0-

141.6 4.7 41.8 41.9 451 1. 1 5.2Acquisition Time (min)


CAL 25

Batch DataData Path D:\2016 Data\JM \OPIATES \081216 \QuantResults \081216jm.batch.bin



Acquisition Method Blood Opiates.m Acquisition limeOperator JM

8/12/2016 11:56 AM

+ TIC MRM (** -> **) CAL 25.d

.g x10 4 -=o 5 _

4.5 -

4 -

3.5 -

3 -

2 -

1.5 -

1 -

0:6 0:8 1 1:2 1:4 1:6 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4:2 4:4 4:6 4:8 6 5:2 5:4 5:6 5:8



Mor-D6 1.43 (1.36-1.51) 292.2 -> 152.1 41780 60.2

Morph (CO -10) 1.45 (1.38-1.52) 286.2 -> 152.1 13244 66.2

OxyMor-D3 1.80 (1.72-1.91) 305.2 -> 287.1 131006 35.3

OxMor 1.82 (1.74-1.92) 302.2-> 284.0 28909 35.0

HyMor-D3 2.31 (2.20-2.43) 289.2 -> 185.0 103563 72.3

HyMor 2.32 (2.21-2.44) 286.2 -> 185.0 22163 67.1

Cod -D6 3.51 (3.34-3.69) 306.2 -> 115.0 54711 118.7

Cod (C0-2) 3.58 (3.41-3.77) 300.2 -> 115.0 16459 104.9

OxCod-D3 4.18 (3.97-4.39) 319.2 -> 301.1 223818 20.4

OxCod 4.24 (4.04-4.46) 316.2 -> 298.2 58041 20.3

HyCod-d3 4.78 (4.54-5.02) 303.2 -> 199.0 140671 40.2

HyCod 4.79 (4.56-5.04) 300.2 -> 199.0 39306 44.1

6 -AM -d3 5.07 (4.82-5.33) 331.2 -> 165.0 113376 46.2

6 -AM (C0-2) 5.08 (4.82-5.33) 328.2 -> 165.0 28647 50.5

Mor-D6

292.2-> 1

4g 0042 1-1

0.8-

0.6-

0.4-

0.2-

o-

52.1 , 292.2 -> 128.0

Ratio = 60.2 (48.2- 72.3)



286.2-> 152.1 , 286.2 ->128.0

t.) 4

2 x10 31 Ratio = 66.2 (511.8 - 76.2)

o

2.5

2

1.5

1

0.5

1 1 1!6 1!8Acquisition Time (min)

Q1 Range ng/mL(48.2-72.3)(50.8-76.2) 24.36

(29.1-43.7)(28.1-42.2) 24.23

(58.6-88.0)(53.9-80.8) 24.22

(95.1-142.6)(85.5-128.2) 24.72

(16.5-24.8)

(16.2-24.3) 24.24

(32.0-48.1)(35.9-53.9) 24.84

(36.4-54.6)(40.0-60.0) 24.44

305.2 -> 287.1 , 305.2 ->230.1

X10 43 2.5

2

1.5

0.5

Ratio = 35.3 (4.1 -43.7)

I1.4 1.6 1.8 2 2.2


OxMor

302.2 ->284.0 , 302.2 -> 227.1

xio 3j

A5-1

4-1

3-1

2-1

1-1

0-1

Ratio = 35.0 (4.1 -42.2)



CAL 25

HyMor-D3 HyMor

289.2-> 185.0 , 289.2-> 157.0

2 x10 41 Ratio = 72.3 (51.6 - 88.0)

-

1.5-1

1-1

0.5-1

0


OxCod-D3

319.2 ->301.1 319.2-> 244.0

2 x10 4_1 Ratio = 20.4 (16.§ -24.8)

a' 3.5,

3-

2.5-

2 -

1.5-

1-1

0.5-1

0-1


6 -AM -d3

331.2-> 165.0 , 331.2 -> 152.0

2 x10 4,1 Ratio = 46.2 (14 - 54.6)

2.5-

2 -

1.5-

1 -

0.5-

0

14.8 ; 5 1 1.2 5.4Acquisition Time (mm)

286.2-> 185.0 , 286.2 ->157.0

xio 3-1 Ratio = 67.1 (9.9 -80.8)• _8 '1

4-

3-I

2-1

1-1

0-1

Cod -D6 Cod (C0-2)

2.2 2.4 2.6Acquisition Time (mm)

OxCod

316.2 ->298.2 , 316.2 -> 241.0

xio4• 1 -o

0.81

0.4-

0.2-

0 -

Ratio = 20.3(16.? -24.3)

3.8 4 4.2 4.4 4.6Acquisition Time (mm)

6 -AM (C0-2)

328.2-> 165.0 , 328.2 -> 211.0

2 x10 3-1 Ratio = 50.5 (4q.0 - 60.0)

o 7 -U

6 -

5-1

4 -

3 -

2 -

1 -

0 -

306.2 -> 115.0 , 306.2 -> 152.0

2 x10 4- 142.6}3

(-) • 1.4-

1.2-

1 -

0.8-

0.6-

0.4-

\••••0.2-

-j /

I I I I• 3.2 3.4 3.6 3.8


HyCod-d3

4!8 5 5!2 5!4Acquisition Time (mm)

303.2-> 199.0 , 303.2 -> 171.0

2 x10 4 I Ratio = 40.2132.0 -48.1)3 3

2.5

2

1.5

1

0.5

0


300.2-> 115.0 , 300.2 -> 152.0

42 x10 3..

o 4 -C.)

3.5-

3 -2.5-

2 -1.5-

1 -0.5-

0 -

Ratio_F- 104-9485.5 _

r I I3.2 3.4 3.6 3.8 4


HyCod

300,2-> 199.0 , 300.2-> 128.0

2 x10 3_1 Ratio = 44.1135.9 - 53.9)

0o 8_1

7

6-5-

4 -

3 -

2 -

01

I 41.6 41.7 41 1.8 4.9 ; :51 51.2Acquisition Time (min)


CAL 100

Batch Data Path D:\2016 DataVM \OPIATES\081216\QuantResults\081216jm.batch.bin

Sample Position P1 -A4

Instrument 6460-6

Acquisition Method Blood Opiates.m

Operator 3M

Sample Name CAL 100

CommentAcquisition Time 8/12/2016 12:10 PM

+ TIC MRM (** -> *1 CAL 100.d

itg x10 4_11

8 -I

7-1

6

5 -

4 -

3 -

2 -

1

k0:6 0:8 1:2 1:4 1:6 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4 4:2 4:4 4:6 4:8 5 5:2 5:4 5:6 5:8



Mor-D6 1.43 (1.36-1.51) 292.2 -> 152.1 39464 60.3

Morph (C0-10) 1.45 (1.38-1.52) 286.2 -> 152.1 53610 63.5

OxyMor-D3 1.82 (1.72-1.91) 305.2-> 287.1 124617 36.4

OxMor 1.83 (1.74-1.92) 302.2-> 284.0 118520 35.2

HyMor-D3 2.31 (2.20-2.43) 289.2 -> 185.0 99607 73.3

HyMor 2.33 (2.21-2.44) 286.2 -> 185.0 91692 67.4

Cod -D6 3.52 (3.34-3.69) 306.2 -> 115.0 53012 118.8

Cod (C0-2) 3.59 (3.41-3.77) 300.2 -> 115.0 64127 106.8

OxCod-D3 4.18 (3.97-4.39) 319.2 -> 301.1 214092 20.7

OxCod 4.25 (4.04-4.46) 316.2 -> 298.2 234101 20.3

HyCod-d3 4.78 (4.54-5.02) 303.2 -> 199.0 137589 40.0

HyCod 4.80 (4.56-5.04) 300.2 -> 199.0 159784 44.9

6 -AM -d3 5.07 (4.82-5.33) 331.2 -> 165.0 108255 45.5

6 -AM (C0-2) 5.08 (4.82-5.33) 328.2 -> 165.0, 116109 50.0

Mor-D6

292.2 ->152.1 , 292.2.> 128.0

x10 4-1 Ratio = 60.3 (442 - 72.3)

0.8

0.6

0.4

0.2

1 I1 1!4 1.6 1Acquisition Time (min)


286.2-> 152.1 , 286.2-> 128.0

xio41 Ratio = 63.5 (50„8 - 76.2)7° 1.2

0.8

0.6

0.4

0.2

1 1:2 1!4 11.6 1:8Acquisition Time (min)

305.2-> 287.1 , 305.2-> 230.1

Id x10 4] Ratio = 36.4 (29,1 -43.7)

02-1

1.5-1

1-1

0.5H


Q1 Range ng/mL(48.2-72.3)

(50.8-76.2) 100.91(29.1-43.7)(28.1-42.2) 101.11(58.6-88.0)

(53.9-80.8) 100.98

(95.1-142.6)(85.5-128.2) 100.35

(16.5-24.8)(16.2-24.3) 101.00

(32.0-48.1)(35.9-53.9) 100.10

(36.4-54.6)

(40.0-60.0) 100.70

OxMor

302.2 -> 284.0 , 302.2-> 227.1

t l X10 4-1 Ratio = 35.2 (28,1 -42.2)

o 2

1.5 -

1 -

0.5-



CAL 100

HyMor-D3

289.2 ->185.0 , 289.2 -> 157.0

g .104 Ratio = 73.3 (5846 - 88.0)

O• 2

1.5-

1 -

0.5-

0 -

2 2:2 2:4 2:6 r


OxCod-D3

319.2 ->301.1 , 319.2-> 244.0

g .1041Ratio 20.7 (16. - 24.8)3O 3 5U •

3-

2.5-

2 -

1.5-

1 -

0.5-

0 -


6 -AM -d3

HyMor Cod -D6

286.2-> 185.0 , 286.2 -> 157.0

X10 4.1

0 21

1.751

1.51

1.25-

0.75-

0.5-

0.25-0 -

Ratio = 67.4 (5 9 -80.8)


OxCod

306.2-> 115.0 , 306.2-> 152.0

ig x10 4 _Ratio 1.18.8.495_1.1.42.6)-

o1.4 -

1.2 -

-

0.8 -

0.2 -

0 -

3:2 3:4 3:6 3:8 rAcquisition Time (min)

HyCod-d3

316.2 ->298.2 , 316.2 -> 241.0

.g x10 4- Ratio = 20.3 (16. - 24.3)8 4 -

° 3.5-

3 -

2.5-

2 -

1 -

0_3:8 4 4:2 4:4 4:6


6 -AM (C0-2)

331.2 -> 165.0 , 331.2 -> 152.0

g .1041Ratio = 45.5 (36,4 - 54.6)

u • 2.51

21

1.51

0.5-

0


328.2-> 165.0 , 328.2 -> 211.0

g .104 I Ratio = 50.0 (40r0 - 60.0)

2.5-

2

1.5

1

0.5

0

I - I I I4.8 5 5.2 5.4


303.2-> 199.0 , 303.2 -> 171.0

.2 x10 4 I Ratio = 40.0 (32.0 -48.1)

C.)


Cod (C0-2)

300.2-> 115.0 , 300.2 -> 152.0

x103 1.6-

1.4-1.2 -

1 -0.8-

0.6-

0.4-

0.2-

0

3:2 3:4 3:6 3:8 :1Acquisition Time (min)

HyCod

300.2-> 199.0 , 300.2 -> 128.0

x10 4_ Ratio = 44.9 35.9 -

O • 3_

2-

1-

i t \



CAL 250



Instrument 6460-6 CommentAcquisition Method Blood Opiates.m Acquisition Time 8/12/2016 12:23 PM

Operator 314

+ TIC MRM

x10 5 -

o• 1.4 -

1.2 -

1 -

0.8-

0.6-

0.4 -

0.2 -

CAL 250.d

'6 : 11 I1 1!4 1.0. 0 8 6 11.8I

2 2.2 2.4 2.6 2.8 3 I3.2 3: I I4 3.6 4.2 4:4 4:64!8 5!2 5:4 5:6 5:8Acquisition Time (min)


Mor-D6 1.43 (1.36-1.51) 292.2-> 152.1 35433 60.4

Morph (C0-10) 1.45 (1.38-1.52) 286.2-> 152.1 123230 65.0

OxyMor-D3 1.80 (1.72-1.91) 305.2-> 287.1 119812 35.9

OxMor 1.82 (1.74-1.92) 302.2 -> 284.0 294573 34.0

HyMor-D3 2.30 (2.20-2.43) 289.2 -> 185.0 93929 71.4

HyMor 2.32 (2.21-2.44) 286.2 -> 185.0 223216 66.9

Cod -D6 3.51 (3.34-3.69) 306.2 -> 115.0 52991 117.2

Cod (C0-2) 3.58 (3.41-3.77) 300.2 -> 115.0 154630 104.9

OxCod-D3 4.18 (3.97-4.39) 319.2 -> 301.1 217092 20.4

OxCod 4.24 (4.04-4.46) 316.2 -> 298.2 600619 20.0

HyCod-d3 4.78 (4.54-5.02) 303.2 -> 199.0 129798 40.3

HyCod 4.79 (4.56-5.04) 300.2 -> 199.0 396558 45.2

6 -AM -d3 5.07 (4.82-5.33) 331.2 -> 165.0 100222 45.9

6 -AM (C0-2) 5.08 (4.82-5.33) 328.2 -> 165.0 278142 50.5

Mor-D6 Morph (C0-10) OxyMor-D3

292.2.> 152.1 , 292.2.> 128.0

,g x10 3- Ratio = 60.4 (4 .2 - 72.3)

o • 8-

7-

451

31

2-

0 .

fl!I 1.2 1.4 1!6 1.8


286.2-> 152.1 , 286.2 -> 128.0

12 x10 4 Ratio = 65.0 (50r8 - 76.2)3

(-) • 2.5

2

1.5

0.5


Q1 Range ng/mL(48.2-72.3)(50.8-76.2) 249.72

(29.1-43.7)(28.1-42.2) 249.66

(58.6-88.0)(53.9-80.8) 249.70

(95.1-142.6)(85.5-128.2) 249.88

(16.5-24.8)(16.2-24.3) 249.69

(32.0-48.1)(35.9-53.9) 249.98

(36.4-54.6)(40.0-60.0) 249.79

305.2 ->287.1 , 305.2 -> 230.1

42 x10 41 Ratio = 35.9 (29.1 -43.7)

O• 2

1.51

0.5 1


OxMor

302.2-> 284.0 , 302.2-> 227.1

42 x10 41 Ratio = 34.0 (4.1 -42.2)3

0 5-

U I I I I1.4 1.6 1.8 2 2.2



CAL 250

HyMor-D3

289.2-> 185.0 , 289.2-' 157.0

12 x10 4- Ratio = 71.4 (5 .6 - 88.0)3o 2-

1.75-1.5-

1

0.750.5

0.250

I I F I2 2.2 2.4 2.6


OxCod-D3

319.2 -> 301.1 , 319.2 -> 244.0

.2 x10 4- Ratio = 20.4(16.5 -24.8)30 35-0 •

3-

2.5-

2 -

1.5-

1-1

0.5-1

0-1


6 -AM -d3

331.2-> 165.0 , 331.2 -> 152.0

12 x10 4 I Ratio = 45.9 (3k4 - 54.6)

4:8 5 5:2 5:4Acquisition Time (mm)

HyMor Cod -D6

286.2-> 185.0 , 286.2 -> 157.0

x10 4 I Ratio = 66.9 (51.9 -80.8)30

4 -

3 -

2 -

1-1

2 2 .12 21.4 2:6Acquisition Time (min)

OxCod

316.2 -'298.2 , 316.2-' 241.0

x10 Ratio = 20.0(16.? -24.3)

0.8-1

0.6-1

0.4-1

0.2-1

0

3: 1 1 :8 .4 4.2 41.4 4 6Acquisition Time (mm)

6 -AM (C0-2)

306.2 -> 115.0 , 306.2 -> 152.0

x10 4l_Ratio.F...1.171495-1,-14214

1.4-

1.2-

1 -

0.8-

0.6-

0.4-

0.2-

0 -

3:2 3:4 3:6 3:8Acquisition Time (min)

HyCod-d3

328.2-' 165.0, 328.2 -> 211.0

2 x10 41 Ratio = 50.5 (40,0 -60.0)

6-

5 .

4 -

3 -

2 -

1-1

0

4.8 5 5.2 5.4Acquisition Time (mm)

303.2 -> 199.0 , 303.2 -> 171.0

(.5 2.5-1

2 -

1.5-

1

0.5

Ratio = 40.3,132.0 -48.1)

1 1 1 1 1 1 1 1


Cod (C0-2)

300.2-> 115.0 , 300.2 -> 152.0

x10 4_. Ratio_=104.9485-5,1284

2.53

2 -

1 -

0 -

3:2 3:4 3:6 3:8 .11Acquisition Time (mm)

FlyCod

300.2-> 199.0 , 300.2-> 128.0

x10 4-1 Ratio = 45.2,,(35.9 - 53.9)g 8-1

7-1

6-1

5 -4 -

3

2 -1-o-



SB before Negative

Batch Data Path D:\2016 DataVIM \OPIATES \081216 \QuantResults \081216jm.batch.bin



Acquisition Method Blood Opiates.m Acquisition Time

Operator JM

SB before Negative

8/12/2016 12:37 PM

+ TIC MRM (**-> **) SB before Negative.d

.g x10 2]

C.> 7 -6.5-

6 -

5.5-5 -

4.5-4 -

3.5-3 -

2.5-2 -

1.5-1 -

0.5-

\

0:6 0:8 21 1:2 1:4 1:6 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4 4:2 4:4 4:6 4:8 5:2 5:4 5:6 5:8Acquisition Time (min)

Compound RT RT Range Transition Area Q1 Q1 Range ng/ml.Mor-D6 (1.36-1.51) 292.2 -> 152.1

Morph (C0-10) (1.38-1.52) 286.2 -> 152.1 nd

OxyMor-D3 (1.72-1.91) 305.2 -> 287.1OxMor (1.74-1.92) 302.2 -> 284.0 nd V

HyMor-D3 (2.20-2.43) 289.2 -> 185.0

HyMor (2.21-2.44) 286.2 -> 185.0Cod -D6 (3.34-3.69) 306.2 -> 115.0

Cod (C0-2) (3.41-3.77) 300.2 -> 115.0OxCod-D3 (3.97-4.39) 319.2 -> 301.1

OxCod (4.04-4.46) 316.2 -> 298.2

HyCod-d3 (4.54-5.02) 303.2 -> 199.0HyCod (4.56-5.04) 300.2 -> 199.0

6 -AM -d3 (4.82-5.33) 331.2 -> 165.06 -AM (C0-2) (4.82-5.33) 328.2 -> 165.0

Mor-D6

292.2-> 152.1 , 292.2.> 128.0

4 xi° 1-1 Nnt Fnund

o0 4-

3

2

1

0

1 I - I1 1.2 1.4 1.6 1.8



286.2-> 152.1 , 286.2-> 128.0

x10 1 Not Found

o4

3 -

2 -

1 -

o -

I1 1.2 1.4 1.6 1.8


305.2 -> 287.1 , 305.2 -> 230.1

x10 1 Not Foung 6

5

4

3

2

1

1:4 1: I6 1.8 22 : 2Acquisition Time (min)

OxMor

nd

nd

nd

nd

nd

302.2-> 284.0 , 302.2 -> 227.1

x10 11 N t Found

o • 5-C.)

-

3 -

2 -

1 -

0-1



SB before Negative

HyMor-D3

289.2-> 185.0 , 289.2-> 157.0

4g oo1]g 6

5-

4-

3 -

2 -

101

Not Found

2:2 2:4 2:6Acquisition lime (min)

OxCod-D3

319.2 -> 301.1 , 319.2 -> 244.0

t l x10 1-I Not Found

g7 -

6 -

-5

7 -

6 -

5 -5-1

4 -

3 -2 -

0-1

3:8 4 4:2 4.4 4.6Acquisition Time (min)

6 -AM -d3

331.2 -> 165.0 , 331.2 -> 152.0

010 1 Not Found

o

4:8 5 5:2 5:4Acquisition lime (min)

HyMor

286.2-> 185.0 , 286.2 -> 157.0

010 11 Not Found

4

3

2

0

F I I I I2 2.2 2.4 2.6


Cod -D6

OxCod

316.2 ->298.2 , 316.2 -> 241.0

x10 21 Not Found

0.7 -0.6 -0.5-10 4

0.3-

0.2-0.1-

0-1

3:8 :4 4:2 4:4 4:6Acquisition Time (min)

6 -AM (C0-2)

306.2-> 115.0 , 306.2 -> 152.0

.g x101 ot Found


HyCod-d3

328.2-> 165.0 , 328.2-> 211.0

x10 idNot Found

O• A

5

4

3

2

1

0-1

4:8 ; 5:2 5:4Acquisition Time (min)

303.2-> 199.0 , 303.2-> 171.0

•P x10 1] Not Found

4

3 -

2 -

1-

o-

4:5 4:6 4:7 4:8 4:9 6 5:1 5:2Acquisition Time (min)

Cod (C0-2)

300.2-> 115.0 , 300.2 -> 152.0

x10 1-1 Not Found


HyCod

300.2-' 199.0 , 300.2 -> 128.0

,g x10 1] Not Found

4:6 4:7 4:8 4:9 6 5:1 5:2Acquisition lime (min)


Negative

Batch Data Path D:\2016 DataVM \OPIATES \081216 \QuantResults \081216jm.batch.bin

Sample Position P1 -A6 Sample Name NegativeInstrument 6460-6 CommentAcquisition Method Blood Opiates.m Acquisition Time 8/12/2016 12:51 PM

Operator 314

+ TIC MRM (**->**) Negative.d

x10 4 1

ca 4.5H

4 -

3.5-

3 -

2.5-

2 -

1.5-

1 - 1

0.5-1

0:6 0:8 1 1:2 1:4 1:6 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4:2 4:4 4:6 4:8 5 5:2 5:4 5:6 51.8


Compound RT RT Range Transition Area Q1 Q1 Range

Mor-D6 1.44 (1.36-1.51) 292.2-> 152.1 43693 60.7 (48.2-72.3)

Morph (C0-10) (1.38-1.52) 286.2 -> 152.1OxyMor-D3 1.81 (1.72-1.91) 305.2-> 287.1 135595 36.3 (29.1-43.7)

OxMor (1.74-1.92) 302.2 -> 284.0HyMor-D3 2.31 (2.20-2.43) 289.2 -> 185.0 105321 72.2 (58.6-88.0)

HyMor (2.21-2.44) 286.2 -> 185.0Cod -D6 3.51 (3.34-3.69) 306.2 -> 115.0 55201 117.2 (95.1-142.6)

Cod (C0-2) (3.41-3.77) 300.2 -> 115.0OxCod-D3 4.18 (3.97-4.39) 319.2 -> 301.1 228469 20.7 (16.5-24.8)

OxCod 4.24 (4.04-4.46) 316.2 -> 298.2 879HyCod-d3 4.78 (4.54-5.02) 303.2 -> 199.0 143298 40.7 (32.0-48.1)

HyCod (4.56-5.04) 300.2 -> 199.06 -AM -d3 5.07 (4.82-5.33) 331.2 -> 165.0 116578 45.8 (36.4-54.6)

6 -AM (C0-2) (4.82-5.33) 328.2 -> 165.0

Mor-D6

292.2.' 152.1 , 292.2-' 128.0

.fg x10 41 Ratio = 60.7 (48.2 -72.3)

O• 10

0.8

0.6

0.4

0.2

0

1:4 1I.6 1.8Acquisition Time (min)


286.2.' 152.1 , 286.2-> 128.0

1g x10 11 Not Found

4-)

3

2 -

1 -

0-

1.I2 1:4 11.6 1-1.8Acquisition Time (min)

305.2 -> 287.1 , 305.2.> 230.1

x10 41 Ratio = 36.3 (2%1 -43.7)z0 2.5

2

1.5

1

0.5

0

I I I I I


OxMor

ng/mL

nd

nd

nd

nd

0.36 P O

nd

nd

302.2-> 284.0 , 302.2 -> 227.1

g x10 1- Not Found

o 5_"

- I- 1 - I I I1.4 1.6 1.8 2 2.2

Acquisition lime (min)


Negative

HyMor-D3

289.2 ->185.0 , 289.2-> 157.0

x10 4d Ratio = 72.2 (56,6 -88.0)

0


OxCod-D3

319.2 ->301.1 319.2-> 244.0

.2 x10 4_ Ratio = 20.7 (16.§ -24.8)3

3-

2 -

1-1

0.5-1

0


6 -AM -d3

331.2-> 165.0 , 331.2-> 152.0

x10 4

2.5-

2 -

1.5 -

1 -

0

Ratio = 45.8 (36..4 - 54.6)


HyMor Cod -D6

286.2-> 185.0 , 286.2-> 157.0

Ig• x10 1] Not Found

2 2:2 2:4 2:6Acquisition Time (mm)

OxCod

316.2 ->298.2 .316.2-> 241.0

.2 x10 2-1 Not Found .."*".g 20

1.75 1

1.5-1

1.25

0.715 1---V Y

0.5

0.25

3:8 4 4 :2 4:4 4:6Acquisition Time (mm)

6 -AM (C0-2)

306.2-> 115.0 • 306.2-> 152.0x10 4

g 1.6(.) 1.4

1.2

1

0.8

0.6

0.4

0.2

0

3:2 3:4 3:6 3:8 rAcquisition Time (mm)

HyCod-d3

328.2 -> 165.0 , 328.2 -> 211.0

,2 x10 11 Not Found3o 6

5

4

3

2

1

0


303.2 -> 199.0 , 303.2 -> 171.0

.2 x10 41 Ratio = 40.7132.0 - 48.1)3 3

2.5

2

1.5

1

0.5

0

! I I I !4.5 4.6 4.7 4.8 4.9 5 5.1 5.2


Cod (C0-2)

300.2-> 115.0 , 300.2-> 152.0

0 4

xlo 1 Not Found

3:2 3:4 3:6 3:8Acquisition Time (mm)

HyCad

300.2-> 199.0 , 300.2 -> 128.0

..2 xl0 1/ Not Found

C.)

4 -

3 -

2 -

1 -

0 -

1- 1 1 1 1 1 1- 1

4.6 4.7 4.8 4.9 5 5.1 5.2Acquisition Time (mm)


Low Control

Batch Data Path D:\2016 Data \lM \OPIATES\ 081216 \QuantResults \081216jm.batch.bin

Sample Position P1 -A7 Sample Name


Acquisition Method Blood Opiates.m Acquisition Time

Operator JM

Low Control

8/12/2016 1:05 PM

+ TIC MRM (** -> **) Low Control.d

x 1 0 4 _1

04 -

3.5-

3 -

2.5-

2 -

1.5-

1 -

0.5-1 77 .1 I I .1 .1

0:6 0:8 1 1:2 1.I4 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4 4:2 4:4 4:6 4:8 4 5:2 5:4 5:6 5:8Acquisition Time (min)

Compound RT RT Range Transition Area Q1 Q1 Range ng/mL

Mor-D6 1.44 (1.36-1.51) 292.2-> 152.1 41659 58.9 (48.2-72.3)Morph (C0-10) 1.45 (1.38-1.52) 286.2-> 152.1 5095 60.0 (50.8-76.2) 9.78

OxyMor-D3 1.80 (1.72-1.91) 305.2 -> 287.1 130803 34.5 (29.1-43.7)OxMor 1.81 (1.74-1.92) 302.2-> 284.0 11050 35.0 (28.1-42.2) 9.49

HyMor-D3 2.30 (2.20-2.43) 289.2 -> 185.0 101264 70.9 (58.6-88.0)HyMor 2.31 (2.21-2.44) 286.2 -> 185.0 8422 69.1 (53.9-80.8) 9.63

Cod -D6 3.51 (3.34-3.69) 306.2 -> 115.0 52496 117.2 (95.1-142.6)

Cod (C0-2) 3.58 (3.41-3.77) 300.2 -> 115.0 6164 105.9 (85.5-128.2) 9.75

OxCod-D3 4.17 (3.97-4.39) 319.2 -> 301.1 209884 21.1 (16.5-24.8)OxCod 4.24 (4.04-4.46) 316.2 -> 298.2 22260 21.3 (16.2-24.3) 9.94

HyCod-d3 4.77 (4.54-5.02) 303.2 -> 199.0 136348 39.7 (32.0-48.1)

HyCod 4.79 (4.56-5.04) 300.2 -> 199.0 14346 46.3 (35.9-53.9) 9.49

6 -AM -d3 5.07 (4.82-5.33) 331.2 -> 165.0 110078 45.7 (36.4-54.6)6 -AM (C0-2) 5.07 (4.82-5.33) 328.2 -> 165.0 10758 49.5 (40.0-60.0) 9.66

Mor-D6

292.2.> 152.1 , 292.2-> 128.0

x10 4 Ratio = 58.9 (48.2 -72.3)-

o

0.6-

0.4-

0.2-

0 -

T T T I I1 1.2 1.4 1.6 1.8



286.2 ->152.1 , 286.2-> 128.0

X10 3-1

° 1.2-

1-1

0.8-

0.6-

0.4-

0.2-

0 -

Ratio = 60.0 (50,8 -76.2)


305.2 -> 287.1 , 305.2 -> 230.1

.g x10 4 I Ratio = 34.5 (4.1 -43.7)g 2.5 -0

2 -

1.5-1

1

0.5-

1 1I.8 2 2!2Acquisition Time (min)

OxMor

302.2 -> 284.0 , 302.2 -> 227.1

t i A 0 3] Ratio = 35.0 (4.1 -42.2)

0

2-1

1.5-1

1

0.5-1

0-1



Low Control

HyMor-D3

289.2-> 185.0 , 289.2-> 157.0

g X10 4-1 Ratio = 70.9 (5§.6 -88.0)

1.5-

1 -

0.5-

0 -

2.2 2.4 2.6Acquisition Time (min)

OxCod-D3

319.2 ->301.1 , 319.2 -> 244.0

.2 x10 4- Ratio = 21.1 (161 -24.8)

g 3.5-

3 -

2.5-

2 -

1.5-

1-1

0.5

0

3:8 :1Acquisition Time (min)

6 -AM -d3

331.2-> 165.0 , 331.2 -> 152.0

x10 4] Ratio >45.7 (31:4 - 54.6)5

2.5-

2 -

1.5-

1-1

0.5-1

0

4.8 5 5.2 5.4


HyMor Cod -D6

286.2-> 185.0 , 286.2 -> 157.0

.2 x10 31 Ratio >69.1 (5q.9 -80.8)o' 2

1.75

1.5

1.25

1

0.75

0.5

0.25

0


OxCod

306.2-> 115.0 , 306.2-> 152.0

x107

1.4

1

0.8 -

0.6-

0.4 -

0.2 -

0

3:2 3.I4 3!6 3.8 IAcquisition Time (min)

HyCod-d3

316.2 ->298.2 , 316.2 -> 241.0

g x10 3 Ratio = 21.3 (16.? - 24.3)

3 -

2 -

1

0.5-1

0-1A

I 1r - 1 - 1- 1-3.8 4 4.2 4.4 4.6


6 -AM (C0-2)

328.2 -> 165.0 , 328.2-> 211.0

0 2.5 -

2 -

1.5

1

0.5-1

0-1

Ratio = 49.5 (41.0 -60.0)

I 4:8 5 5.I2 5!4Acquisition Time (min)

303.2-> 199.0 , 303.2 -> 171.0

3X10 4

2.5

2

1.5

1

0.5

0

Ratio= 39.7,(32.0 -48.1)

4!5 4.6 4!7 4!13 4!9 5 51.1 5I.2Acquisition Time (min)

Cod (C0-2)

300.2 ->115.0 , 300.2 -> 152.0

o311.6-

1.4 -

1.2-

1 -

0.8-

0.6-

0.4 -

0.2-

o-

_ Ratio..F_105.9.05.5,12R 2)

- 1 1 1 1 13.2 3.4 3.6 3.8 4


HyCod

300.2-> 199.0 , 300.2 -> 128.0

2 x10 31 Ratio = 46.3,(35.9 -53.9)3 31

2.5-

2 -

1.5-

1 -

0.5-1

0-1

4.6 4.7 4.8 4.9 5 5.1 5.2Acquisition lime (min)


SB before Medium Control

Batch Data Path D:\2016 Data \KOPIATES\081216\QuantResults\081216jm.batch.bin



Acquisition Method Blood Opiates.m Acquisition limeOperator 3M


8/12/2016 10:00 PM

+ TIC MRM (** -> **) SB before Medium Control.d

p x10

7 -

6.5-6 -

5.5-5 -

4.5-4 -

3.5-3 -

2.5-2 -

1.5-1 -

0.5-0:6 0:8 1:2 1:4 1:6 1:8 2:2 2 .'4 2:6 2:8 3 3:2 3:4 3:6 3:8 4 4 .'2 4:4 4:6 4:8 5 5:2 5.4 5:6 5:8



Morph (C0-10) (1.38-1.52) 286.2 -> 152.1 nd

OxyMor-D3 (1.72-1.91) 305.2 -> 287.1

OxMor (1.74-1.92) 302.2 -> 284.0 nd

HyMor-D3 (2.20-2.43) 289.2 -> 185.0

HyMor (2.21-2.44) 286.2 -> 185.0Cod -D6 (3.34-3.69) 306.2 -> 115.0

Cod (C0-2) (3.41-3.77) 300.2 -> 115.0OxCod-D3 (3.97-4.39) 319.2 -> 301.1

OxCod (4.04-4.46) 316.2 -> 298.2HyCod-d3 (4.54-5.02) 303.2 -> 199.0

HyCod (4.56-5.04) 300.2 -> 199.06 -AM -d3 (4.82-5.33) 331.2 -> 165.0

6 -AM (C0-2) (4.82-5.33) 328.2 -> 165.0

Mor-D6

292.2-> 152.1 , 292.2.> 128.0

x10 1] Not Found

U 4-

3

2

1

0

I I T I1 1.2 1.4 1.6 1.8


Morph (C0-10)

286.2.> 152.1 , 286.2 -> 128.0

xio i] Not Found3

0 4

3

2

1


OxyMor-D3

305.2 ->287.1 , 305.2-> 230.1

.g x10 1 Not F und

4

3

2

1

0

I I - F I I1.4 1.6 1.8 2 2.2


nd

nd

nd

nd

nd

OxMor

302.2 -> 284.0 , 302.2 ->227.1

xlo 11Not Found

4-1

3

2

1:4 1:6 1:8 2 2:2Acquisition Time (min)



HyMor-D3 HyMor

289.2-> 1

x 1 0 1

5-1

4 -

3 -

2 -

1 -

0 -

85.0 , 289.2-> 157.0

Not Found

2!2 21.4 21.6 r


OxCod-D3

319.2 ->301.1 , 319.2 -> 244.0

x10 21 Not Found

0.8-

0.6

0.4

0.2

0

I I.8 43 .2Acquisition Time (mm)

6 -AM -d3

331.2-> 165.0 , 331.2 -> 152.0

,g x10 11 Not Found

3 5

3

2

5!2 51.4Acquisition Time (min)

Cod -D6

286.2-> 185.0 , 286.2-> 157.0

42 x10 1 Found- cs o•-•-•

O 4 -

3.5 -3 -

2.5-

2 -1.5-

1-10.5-1

0-1


OxCod

306.2-> 115.0 , 306.2-> 152.0

12 x10 1 -I Not Eound

(1 4

I I T I3.2 3.4 3.6 3.8


liyCod-d3

316.2 ->298.2 , 316.2-> 241.0

o 8

7

654

32

I I I I - I-3.8 4 4.2 4.4 4.6


6 -AM (C0-2)

328.2 ->165.0 , 328.2 -> 211.0

x10 1 Not Found

5--

4 -

3 -

2 -

1-1

0

'8 4 I4. 5.2 5.4Acquisition Time (min)

303.2 ->199.0 , 303.2 ->171.0

x10 1-1 Not Found

0 5

4

3

2

0

4.5 4.6 4.7 4.8 4.9 5 5.1 5.2Acquisition Time (mm)

Cod (C0-2)

300.2-> 115.0 , 300.2 -> 152.0

42 510 11 Not Found

0 • 43.5

32.5

21.5

10.5

0

3!2 3.4 3.I6 3:8Acquisition Time (min)

HyCod

300.2-> 199.0 , 300.2 -> 128.0

.g 510 1 Not Found

• 4

3 -1

2 -1

1

0-1



Medium Control


Sample Position P1 -A8 Sample Name Medium Control

Instrument 6460-6

Acquisition Method Blood Opiates.m

Operator 3M

Comment

Acquisition Time 8/12/2016 10:14 PM

+ TIC MRM (** -> **) Medium Control.d

ig X10 4 -= 6 5 -0 •

6 -

5.5-5 -

4.5-4 -

3.5-3 -

2.5-2 -

1.5-1 -

0.5-

0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 4.2 4.4 4.6 4.8 5 5.2 5.4 5.6 5.8Acquisition Time (mm )

Compound RT RT Range Transition Area Q1 QI Range ng/mLMor-D6 1.47 (1.36-1.51) 292.2 -> 152.1 41306 60.3 (48.2-72.3)

Morph (C0-10) 1.48 (1.38-1.52) 286.2 -> 152.1 26073 65.1 (50.8-76.2) 47.72

OxyMor-D3 1.88 (1.72-1.91) 305.2 -> 287.1 132931 36.0 (29.1-43.7)

OxMor 1.89 (1.74-1.92) 302.2-> 284.0 60745 33.8 (28.1-42.2) 49.50

HyMor-D3 2.36 (2.20-2.43) 289.2 -> 185.0 105362 70.5 (58.6-88.0)HyMor 2.38 (2.21-2.44) 286.2 -> 185.0 44135 69.6 (53.9-80.8) 46.83

Cod -D6 3.58 (3.34-3.69) 306.2 -> 115.0 54030 117.1 (95.1-142.6)Cod (C0-2) 3.66 (3.41-3.77) 300.2 -> 115.0 32434 105.2 (85.5-128.2) 49.37

OxCod-D3 4.28 (3.97-4.39) 319.2 -> 301.1 222122 20.8 (16.5-24.8)OxCod 4.34 (4.04-4.46) 316.2 -> 298.2 115224 20.6 (16.2-24.3) 48.31

HyCod-d3 4.83 (4.54-5.02) 303.2 -> 199.0 140538 40.6 (32.0-48.1)HyCod 4.85 (4.56-5.04) 300.2 -> 199.0 76555 45.3 (35.9-53.9) 47.90

6 -AM -d3 5.10 (4.82-5.33) 331.2 -> 165.0 108667 45.9 (36.4-54.6)6 -AM (C0-2) 5.10 (4.82-5.33) 328.2 -> 165.0 54361 50.9 (40.0-60.0) 47.81

Mor-D6

2922-> 152.1 , 292.2-> 128.0

x10 4 Ratio = 60.3 (48. - 72.3)1

0.8

0.6-

0.4-

0.2-

0 -

ii

1.2 111 .4 11.6 11.8Acquisition Time (min)


286.2-> 152.1 , 286.2-> 128.0

12 x10 3-I Ratio = 65.1 (50.5 - 76.2)

0° 6

5

4

3

2

11 11.2 1.I4 1:6 1:8Acquisition Time (min)

305.2 -> 287.1 , 305.2 -> 230.1

x10 41 Ratio = 36.0 (29.1; 43.7)

2.5-1

2-1

1.5-1

1-1

0.5-1

0-1

1!4 1I.6 1:8 2 2:2Acquisition Time (min)

OxMor

302.2-> 284.0 , 302.2 -> 227.1

X10 41.2 -

1 -

0.8-

0.6-

0.4

0.2-

Ratio = 33.8 (28.1 -42.2)

I V I I I



Medium Control

HyMor-D3

289.2 ->185.0 , 289.2 -> 157.0

2 x10 4 Ratio = 70.5 (58.q- 88.0)g 2.51

2

1.5

1

0.5

0

2 2:2 2:4 2:6


OxCod-D3

319.2 -> 301.1 .319.2 -> 244.0

2 x10 4 _ Ratio = 20.8 (16.5 - ?4.8)

0 • 3.5-

3 -

2.5-

2 -

1.5-

1-1

0.5-1

0

1 3:8 4 4:2 4:4 4:6Acquisition Time (mm)

6 -AM -d3

331.2-> 165.0 , 331.2 -> 152.0

2 x10 4 _ Ratio = 45.9(36.4 - 54.6)

O• 2.5-

2 -

1.5-

1-1

0.5-1

0

4:8 5 5:2 5:4Acquisition Time (mm)

HyMor Cod -D6

286.2-> 185.0 , 286.2 -> 157.0

g .10 41 Ratio = 69.6NI - 80.8)

O • 1 -0

0.8

0.6

0.4

0.2

0

2 2:2 2:4 2:6Acquisition Time (mm)

OxCod

316.2 ->298.2 , 316.2 -> 241.0

2 x1041 Ratio=20.6(16.2 -

O• 1.75-

1.5

1.25.

0.75-

0.5 -

0.25 -

0-1

306.2 -> 115.0 , 306.2 -> 152.0

2 x10 41...Ratio g_117.1.(95.1.".1.42-6).

(,) 1.4 -

1.2 -

1 -

0.8-

0.6-

0.4-

0.2-

0

3:2 3:4 3:6 3:8 r


HyCod-d3

3:8 4 4 :2 4:4 4:6Acquisition Time (min)

6 -AM (C0-2)

328.2-> 165.0 , 328.2 -> 211.0

2 x10 4 d Ratio = 50.9 (4o.p -60.0)g 1.4-1

1.2-1

1

0.8

0.6

0.4

0.2

0

4.8 5 5.2 5.4Acquisition Time (mm)

303.2 ->199.0 , 303.2 -> 171.0

2 x10 41 Ratio = 40.6 (40 - 48.1)

3 310 -

2.5 -

2 -

1.5 -

1 -

0.5-

0 -

4.5 4.6 4.7 4.8 4.9 5 5.1 5.2Acquisition Time (mm)

Cod (C0-2)

300.2-> 115.0 , 300.2 -> 152.0

2 x10 3- ...Ratio.s..1.05.2.485.5.,..1282).3 8 -

00

7 -

6 -

5 -

4 -

3 -

2 -

1-1

0

- E I 13.2 3.43.4 3.6 3.8 4


HyCod

300.2 ->199.0 , 300.2 -> 128.0

2 x104_1 Ratio = 45.3 (3p.9 - 53.9)SR 1.6

1.4-1

1.2-1

1-

0.8-0.6-0.4-

0.2-.

4:6 4:7 4:8 4:9 5 5:1 5:2Acquisition Time (mm)



Batch Data Path D:\2016 Data VM \OPIATES \081216 \QuantResults\081216jm.batch.bin

Sample Position Vial 1 Sample Name SB before High Control

Instrument 6460-6 CommentAcquisition Method Blood Opiates.m Acquisition Time 8/ 13/2016 4:51 AM

Operator 31%1

+ TIC MRMX1 0 2 _

o 7 _

6 -

5 -

4 -

3 -

2 -

1.5- -

1 -

(** -> SB before High Control.d

0:6 0:8 11 1:2 1:4 1:6 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4 4:2 4:4 4:6 4:8 B 5:2 5:4 5:6 5:8Acquisition Time (min)


Morph (C0-10) (1.38-1.52) 286.2 -> 152.1 nd

OxyMor-D3 (1.72-1.91) 305.2 -> 287.1OxMor (1.74-1.92) 302.2 -> 284.0 nd

HyMor-D3 (2.20-2.43) 289.2 -> 185.0

HyMor (2.21-2.44) 286.2 -> 185.0Cod -D6 (3.34-3.69) 306.2 -> 115.0

Cod (C0-2) (3.41-3.77) 300.2 -> 115.0

OxCod-D3 (3.97-4.39) 319.2 -> 301.1OxCod (4.04-4.46) 316.2 -> 298.2

HyCod-d3 (4.54-5.02) 303.2 -> 199.0HyCod (4.56-5.04) 300.2 -> 199.0

6 -AM -d3 (4.82-5.33) 331.2 -> 165.06 -AM (C0-2) (4.82-5.33) 328.2 -> 165.0

Mor-D6

292.2-' 152.1 , 292.2-' 128.0

0 4

SA 510 1 Not Found

1 1!4 1!6 1.8Acquisition Time (min)


286.2-> 152.1 , 286.2 -> 128.0

4A 510 1 Not Found

4

3

2-1

1-1

0-1

T I I I


305.2-> 287.1 , 305.2 -> 230.1

.10id Not Found

6-1

5 -

4 -

3 -

2 -I

0-1

I I T1.4 1.6 1.8 2 2.2


OxMor

nd

nd

nd

nd

nd

T h I I I I1.4 1.6 1.8 2 2.2




HyMor-D3

289.2-> 185.0 , 289.2-> 157.0

2 x10 1 Not Found

_ A4 -

3-

2 -

1-1


OxCod-D3

319.2-> 301.1 , 319.2 -› 244.0

2 x10 2- Not Found-

o

0.4

0.1 -

0 -

3:8 4 4 :2 4.I4 4!6Acquisition Time (min)

6 -AM -d3

331.2-> 165.0 , 331.2-> 152.0

2 x10 1 I Not Found3o

4 -

3-

2 -

1 -

0-1

4:8 5 5!2 5.4Acquisition Time (min)

HyMor Cod -D6

286.2-> 185.0 , 286.2-> 157.0

x1011Not Found

O • 4


OxCod

306.2-> 12 x10 1_

4 -

3 -

2 -

1 -

0 -

15.0 , 306.2 ->152.0

Not Found

31.2 3.I4 3:6 3.I8 rAcquisition Time (min)

HyCod-d3

316.2 ->298.2 , 316.2-> 241.0

x10 2-I Not Found

0.8-

0.6-

0.4

0.2-1

0-1

T h

3.8 .11 4:2 4:4 4:6Acquisition Time (min)

6 -AM (C0-2)

328.2-> 165.0 , 328.2 ->211.0

42x10 Not Found3O 6

5

4

3

2

1

0


303.2-> 199.0 , 303.2 -> 171.0

2 X10 ] Not Found

5-1

4

3

2

1

0

4:5 4:6 4:7 4:8 4:9 5 5 :1 5:2Acquisition Time (min)

Cod (C0-2)

300.2 -> 115.0 , 300.2 -> 152.0

42 x10 1- Not Found

84 -

3-

2 -

1 -

0 -

3:2 3:4 3:6 31.8 :4Acquisition Time (min)

HyCod

300.2-> 199.0 , 300.2 -> 128.0

xio 11 Not Found,,„

0

1 1 1 1 1 1 1 1



High Control

Batch Data Path

Sample Position

Instrument

Acquisition Method

Operator

D:\2016 DataVM \OPIATES \081216 \QuantResults\081216jm.batch.bin

P1 -A9

6460-6

Blood Opiates.m

31,1

Sample Name

Comment

Acquisition Time

High Control

8/13/2016 5:05 AM

+ TIC MRM (**->**) High Control.d

V x10

0:6 0:8 1 1:2 1:4 1:6 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4 4:2 4:4 4:6 4:8 5:2 5:4 5:6 5:8



Mor-D6 1.47 (1.36-1.51) 292.2-> 152.1 40780 60.6

Morph (C0-10) 1.49 (1.38-1.52) 286.2-> 152.1 102346 68.6

OxyMor-D3 1.89 (1.72-1.91) 305.2-> 287.1 133268 36.0

OxMor 1.91 (1.74-1.92) 302.2-> 284.0 225275 37.8

HyMor-D3 2.37 (2.20-2.43) 289.2 -> 185.0 104821 70.8

HyMor 2.38 (2.21-2.44) 286.2 -> 185.0 187681 67.2

Cod -D6 3.59 (3.34-3.69) 306.2 -> 115.0 55814 116.3

Cod (C0-2) 3.67 (3.41-3.77) 300.2 -> 115.0 131456 106.4

OxCod-D3 4.28 (3.97-4.39) 319.2 -> 301.1 234320 20.3

OxCod 4.35 (4.04-4.46) 316.2 -> 298.2 477637 20.6

HyCod-d3 4.84 (4.54-5.02) 303.2 -> 199.0 142941 40.8

HyCod 4.85 (4.56-5.04) 300.2 -> 199.0 325667 44.6

6 -AM -d3 5.10 (4.82-5.33) 331.2 -> 165.0 109504 45.6

6 -AM (C0-2) 5.11 (4.82-5.33) 328.2 -> 165.0 223848 51.4

Mor-D6

292.2 -'152.1 .292.2 -'128.0x10 4] Ratio = 60.6 (484 -72.3)

0.8-

0.6-

0.4-

0.21

1 1:2 1:4 1:6 1:8Acquisition Time (min)


286.2-' 152.1 , 286.2 -> 128.0

)2 x10 41 Ratio = 68.6 (504 - 76.2)

02-1

1.5-

1 -

0.5-

1 1:2 1:4 1:6 1:8Acquisition lime (min)

Q1 Range ng/ml.(48.2-72.3)(50.8-76.2) 182.83

(29.1-43.7)(28.1-42.2) 175.52

(58.6-88.0)(53.9-80.8) 191.25

(95.1-142.6)(85.5-128.2) 199.47

(16.5-24.8)(16.2-24.3) 185.79

(32.0-48.1)(35.9-53.9) 190.24

(36.4-54.6)(40.0-60.0) 187.18

305.2 -> 287.1 , 305.2 -> 230.1

2 X10 4 Ratio = 36.0 (29.1A43.7)

o • 2.5

2 -

1.5

1-

0.5-

1:4 1:6 1:8 2 2:2Acquisition Time (mm)

OxMor

302.2 -> 284.0 , 302.2-> 227.1

2 X10 4

O • 4 -3.5-

3 -2.5-

2 -

1.5-1 -

0.5-o-

Ratio = 37.8(28.1 : 42.2)

1:4 1:6 1:8 2 2 :2Acquisition lime (min)


High Control

HyMor-D3

289.2 -> 185.0 , 289.2-> 157.0

2 x10 4 I Ratio >70.8 (58.6,-88.0)

U2-1

1.5-1

1-1

0.5-1

0

2:2 2:4 2I.6Acquisition Time (min)

OxCod-D3

319.2 -> 301.1 , 319.2 -> 244.0

x10 4- Ratio = 20.3 (16.5 - g4.8)

3 4 -° 3.5-

3 -

2.5-

2 -

1 -

0 -

3:8 :4 4I.2 4!.4 4!6Acquisition Time (mm)

6 -AM -d3

331.2-> 165.0 , 331.2 -> 152.0

Ig X10 4 _1 Ratio >45.6 (364 - 54.6)

o 2.5-

2 -

1.5-

1 -

0.5

0-

I I 1-4.8 5 5.2 5.4


HyMor Cod -D6

286.2-> 185.0 , 286.2-> 157.0

p X10 4- Ratio = 67.2 (53.9 -80.8)3

o • 4 -3.5 ___________

3 -2.5

2 -

1 -

0 -

2 2!2 2.4 2.I6Acquisition Time (min)

OxCod

306.2-> 115.0 , 306.2.> 152.0

X10 4 -

1.6-1.4-

1.2-

1 -

0.8 -

0.6-

0.4 -

0.2 -

0 -

_Ratio.=_116.3495_1 -1424

.1

3.I2 3:4 3!6 3.8Acquisition Time (min)

HyCod-d3

316.2 ->298.2 , 316.2 -> 241.0

2 x10 4-1 Ratio = 20.6(16.2 - Z4.3)02 8

• 7

65

4

3

2

1

3.8 :4 4:2 4I.4 4.6Acquisition Time (min)

6 -AM (C0-2)

328.2-> 165.0 , 328.2-> 211.0

2 x10 4_1 Ratio = 51.4 (46.p-60.0)

5

4

3

2

1

0


303.2 -> 199.0 , 303.2 -> 171.0

2 x10 4-1 Ratio = 40.8 (32.0 -48.1)

0 3_1

2.5-

2 -

1.5 -

1 -

0.5 -

0


Cod (C0-2)

300.2-> 115.0 , 300.2-> 152.0

2 x10 4-1_.Ratio _10f2.4485.5,1282)_

0 3

2.5

2

1.5

1

0.5

0

I3.2 3: I I4 3.6 3.8 :4Acquisition Time (min)

HyCod

300.2 ->199.0 , 300.2 ->128.0

2 x10 41 Ratio = 44.6 (19 - 53.9)-I

0 I

6 -

5 -

4 -

3 -

2 -

1 -

0 - ii -



Final Solvent Blank

Batch Data Path

Sample Position

InstrumentAcquisition Method

Operator

D:\2016 DataVM \OPIATES\081216\QuantResults\081216jm.batch.bin

Vial 1

6460-6

Blood Opiates.m

3M

Sample Name

Comment

Acquisition Time

Final Solvent Blank

8/13/2016 5:19 AM

+ TIC MRM (**-> **) Final Solvent Blank.d

18 x10 2 -co 7 -0

6.5-6 -

5.5-5 -

4.5-4 -

3.5 -3 -

2.5-2 -

1.5 -1 -

0.5-0:6 0:8 4 1:2 1:4 1:6 1:8 2 2:2 2:4 2:6 2:8 3 3:2 3:4 3:6 3:8 4 4:2 4:4 4:6 4:8 4 5:2 5:4 5:6 5:8


Compound R I RI Range Transition Area Q1 Q1 Range ng/mLMor-D6 (1.36-1.51) 292.2 -> 152.1

Morph (C0-10) (1.38-1.52) 286.2 -> 152.1 nd

OxyMor-D3 (1.72-1.91) 305.2 -> 287.1OxMor (1.74-1.92) 302.2 -> 284.0 nd

HyMor-D3 (2.20-2.43) 289.2 -> 185.0

HyMor (2.21-2.44) 286.2 -> 185.0Cod -D6 (3.34-3.69) 306.2 -> 115.0

Cod (C0-2) (3.41-3.77) 300.2 -> 115.0OxCod-D3 (3.97-4.39) 319.2 -> 301.1

OxCod (4.04-4.46) 316.2 -> 298.2

HyCod-d3 (4.54-5.02) 303.2 -> 199.0HyCod (4.56-5.04) 300.2 -> 199.0

6 -AM -d3 (4.82-5.33) 331.2 -> 165.06 -AM (C0-2) (4.82-5.33) 328.2 -> 165.0

Mor-D6

292.2-> 1

42 xlcol

U

52.1 , 2922 -> 128.0

Not Foutx

3 -

2-

1-

0 -



286.2-> 152.1 , 286.2-> 128.0

2 x10 1 Not Found30(,) 4-

3.5-i

1 11.2 11.4 1:6 11.8Acquisition Time (min)

305.2-> 287.1 , 305.2 -> 230.1

.10 id Not Found

0 6

54

3

2

1

0

I I I1.4 1.6 1.8 2 2.2


nd

nd

nd

nd

nd

OxMor

302.2-> 284.0 , 302.2 ->227.1

x10 1-1 Not Fo„und

5

4

3

2

1-

o-

1'.4 11.6 1:8 2 21.2Acquisition Time (min)


Final Solvent Blank

HyMor-D3

289.2-> 185.0 , 289.2 ->157.0

2 x10 11Not Found

A4

3

2

1

04•4601#

2 21.2 2.14 2:6 r


OxCod-D3

319.2 ->301.1 , 319.2-> 244.0

2 x10 21 Not Found

u° 0.80.70.6-10.5H

0.30.20.1

0


6 -AM -d3

331.2 -> 165.0 , 331.2 -> 152.0

x101 Not Foundo

4-1

3

2

1

0

4:8 5 5 :2 5!4Acquisition Time (min)

HyMor Cod -D6

286.2-> 185.0 , 286.2 -> 157.0

2 x10 1 Not Found

0 4 -

3 -

2 -

1 -

0


OxCod

316.2 ->298.2 , 316.2 -> 241.0

x10 Not Found

8 8 Af17 - V " W6 -5 -4 -3

2

1

306.2-> 115.0 , 306.2-> 152.0

.2 x10 1 Not Found

0 4

3

2

1

0

1.2 3:4 3:6 3:3 8 IAcquisition Time (min)

HyCod-d3

3:8 .14 4:2 4:4 4:6Acquisition Time (min)

-AM (C0-2)

328.2-> 165.0 , 328.2 -> 211.0

g xlo 1 Not Found6

5 -

4 -

3 -

2 -

1 -

0 -

4:8 55 : 25 :4


303.2 -> 199.0 , 303.2 -> 171.0

.2 x10 1 Not Found

0 5

4

3

2

1-

o-

4:5 4:6 4:7 4:8 4:9 5 5:1 5:2Acquisition Time (min)

Cod (C0-2)

300.2-> 115.0 , 300.2 -> 152.0

42 x10 11 Not Foundz0 4

3 -

2 -

1 -

0 -


FlyCod

300.2-> 199.0 , 300.2-> 128.0

2 x10 1_1 Not Found

4-1

3-1

2-1

0-1

I 4:6 4: 17 4:8 4:9 4 5.1 5:2Acquisition Time (min)


jm calibrators:...5 cal 5 p1442 blood opiates.m cal 5.d calibration 2 6 cal 25 p1 -a3 blood...

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