john la salle - opening plenary
DESCRIPTION
The “Barcode Blitz”: accelerating the targeted capture of barcode dataTRANSCRIPT
The “Barcode Blitz”: accelerating the targeted capture of barcode data
John La Salle, Beth MantleCSIRO Ecosystem Sciences, Canberra, Australia
Jeff Webb, Rodolphe Rougerie, Paul HebertBiodiversity Institute of Ontario, Guelph Canada
The “Barcode Blitz” - ANIC Lepidoptera
• A coordinated assault for targeted and priority capture of barcode data on ANIC Lepidoptera
• Has shown it is possible to gather large amounts of data quickly
• Built a substantive library for a megadiverse group at continental scale in a short time frame
• Fits into / supports• Large scale digitisation efforts• Research outcomes• Operational outcomes• Rapid species discovery
The “Barcode Blitz”
Two visits from BIO / Guelph team to ANIC
5 people x 5 weeks each
Jaclyn McCormick Paul Hebert
Jayme Sones Rodolphe Rougerie
Stephanie deWaard Jeff Webb
Cirsty Carr Jeremy deWaard
Renee Labbee Patrick Strutzenberger
Valerie Levesque-Beaudin Mari Kekkonen
The “Barcode Blitz”
• In 10 weeks
• 28,000 specimens processed• 8,000 species • Databased, geocoded, imaged on site• Leg taken• Sequenced in Canada
Assembly line processing
Assembly line processing
Selecting specimens
• Multiple specimens per OTU
Selecting specimens
• From collection to array box
Specimens into array boxes
• Each space numbered
• Aligns with number of wells in lysis plate
• Position of every specimen taken from collection marked
Specimens databased and geocoded
Specimens into processing boxes
• Specimens labelled as they are processed
• BOLD label
• ANIC label
Every specimen photographed
Leg removed for barcoding
• Each lysis plate gets unique label that ties it back to array box
Specimens back to collection
• Specimens returned to exactly where they were taken
The “Barcode Blitz”
• In 10 weeks
• 28,000 specimens processed• 8,000 species • Databased, geocoded, imaged on site• Uploaded to BOLD within 24 hours• Leg taken• Sequenced in Canada
The “Barcode Blitz” - what did we achieve
Despite an average age of 30 years, COI sequences were recovered from more than 95% of the specimens
The “Barcode Blitz” - what did we achieve
Provided the first continent-wide data set for a megadiverse insect group in Australia within a short period of time (about 90% of named Lepidoptera)
Provides a model for inclusion in other initiatives aimed at the high-throughput accumulation of priority data sets.
Establishes the great value of existing natural history collections as a basis for fast tracking the development of comprehensive DNA barcode libraries.
Despite popular opinion …
The “Barcode Blitz” - Australian benefits
•Digital records in ANIC, ALA, BOLD
(28,000 databased, geocoded, imaged specimens)
The “Barcode Blitz” - results
• What did we achieve
The “Barcode Blitz” - results
• What did we achieve
The “Barcode Blitz” - Australian benefits
• ANIC barcoding blitz activity is our clearest and most positive example of an accelerated and serious attempt to digitise a significant representative sample of a national collection.
• Presenting the results in their fullness (including the capability of this process to highlight the range of undescribed diversity) would be a key element in a case to support a major national digitisation activity
One example of international benefit
• A message from South Africa
“Note that the mitochondrion sequences we obtained from the groundnut leaf miner in South Africa matched 100% with the soybean moth Approaerema simplexella PS1 from Australia on the BOLD system, and that is the reason we are interested in the soybean moth in Australia.”
The “Barcode Blitz” - Australian benefits
• A searchable digital library for Australian Lepidoptera (including images and barcoding)
• Foundation for a variety further studies• Facilitate identification• Able to connect life histories• Informs systematics, biodiversity, biosecurity
• Foundation for Rapid Taxonomy efforts
Rapid Taxonomy of Australian Xylorictidae
Generations of investment in morphology (through putative species sorted in the collection)
Estimated 250 new species – with most of them already sorted to morphospecies
Rapid Taxonomy of Australian Xylorictidae
• Group with no active specialist (last description in 1964) – no revisions on horizon.
• Other major museums have made types of Australian xylorictids available for barcoding/study
• Become a project for Patrick Strutzenberger (PhD student, Guelph)• DNA barcodes will be used to verify/validate morphospecies in
collection• Produce standardized descriptions + images + barcodes (?plus
genitalia)• Can progress taxonomic knowledge of this group rapidly
• GOAL: 250 species described in 2 years, with 100 done by this time next year
Accelerated Species Discovery
taxonomist Accelerated phenomics
Accelerated DNA
Accelerated species discovery and
description
Crowdsourcing citizen
scientists
Contact UsPhone: 1300 363 400 or +61 3 9545 2176
Email: [email protected] Web: www.csiro.au
Thank you
Funding:Genome CanadaCSIRO Transformational Biology
Bio Team from Guelph:Jaclyn McCormick Rodolphe Rougerie
Jayme Sones Jeff Webb
Stephanie deWaard Jeremy deWaard
Cirsty Carr Patrick Strutzenberger
Renee Labbee Mari Kekkonen
Valerie Levesque-Beaudin