Glutathione Peroxidase 3

Kadiatu Kamara

Research Summary

• This research seeks to understand the relationship between chronic kidney disease and low levels of glutathione peroxidase 3. We hypothesized that patients with certain stage of chronic kidney disease witnessing a deficiency in glutathione peroxidase 3 (GPx-3) face a chance of uremic cardiomyopathy. With this research we showed:

I. The function and role of the protein GPx-3;II. Experimental data on western blots, chronical studies on mouse,

GPx-3 Elise to understand the protein; andIII. Further studies and limitations.

GPx-3Also known as extracellular glutathione peroxidase is a antioxidant that is made in the basolateral compartment of the kidney. It is transported to the systemic circulation with functions in the plasma and on circulating platelets.

Methods

• Western blot to identify multiple circulating proteins.• Mouse studies to isolate the

exclusive effect of GPx-3.• GPX-3 (human) Elisa Kit used for in

vitro quantitative determination.

Cystatin

Complement Factor D

Protein 6

Retinol binding protein

GPx-3L - Selectin

What does this mean for the future?!

Thank You