CURRICULUM VITAE

NAME Katherine Louise HayesD.O.B 5.6.71

ADDRESS 10, Goldfinch Drive Cottenham Cambridge

CB24 8XY

Mobile No 0779 6903850E mail hayeskl@btinternet.com

QUALIFICATIONS:

UNIVERSITY OF PORTSMOUTH

BSc (Hons) Molecular Biology (2ii)Full time (1991 – 1993)

Final year 8 week project undertaken at Tenovus Laboratories, Southampton General Hospital. The aim was to optimise a PCR reaction with new primers for HLA class II subtyping comparing the results to the current serological tests.

MSc Biomedical Science (Clinical Biochemistry)Part time (1996 – 1999)

Final year project undertaken at place of work (CAMR, Porton Down, Salisbury).Aim - to optimise cell culture reactor conditions for production of a therapeutic protein from a recombinant cell line.

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WORK EXPERIENCE:

1. Current Post: Abcam, Cambridge Scientific support2006 to present

Scientific Support Supervisor Jul 2010 to present

Line management/supervisory Line management 4-5 scientific support specialists

(Training, coaching, setting objectives, personal development, recruitment) Writing and revising team training manuals and SOP’s. Chairing team meetings. Reporting team monthly key performance indicators (KPI). Present to management.

Working with IT to initiate and set up new reporting tools and dashboards.Proficient with Excel for complex reporting. Some knowledge of SQL.

Coordinating team training for antibody applications/laboratory Coordinating Customer Services and Regional sales team cross training.

Other responsibilities UK office new staff induction presentations - introduction to antibodies and other products. Antibody application presentations at international Abcam roadshow (IHC, ICC, WB). Western blot, IHC and general training presentations to distributors at distributor training days. Supplier visits – observe product demonstrations and discuss technical support with supplier.

Provide feedback training to the team. Coordinate training resources for these particular products. Attendance of UK and international conferences and also Abcam conferences. Attendance on

marketing booth.

Customer core work: (50% of time) Answering international customer enquiries and complaints by telephone, website and email.

Including troubleshooting of complaints. Recording and initiating actions from complaints (internal processes in place).

Scientific reviewing of 'Abreview' customer experimental data (feedback). Correspondence with suppliers regarding customer enquiries and product QC issues. Correspondence with international distributors.

Senior Scientific Support Specialist Jan 2009 – Jun 2010Customer core work: (70% of time)

Scientific Support Specialist Jul 06 - Dec 08Customer core work: (80% of time)

Previous project work: Product recalls/notifications - Initiated and worked with IT to set up an automated system to email

customers for recalls. Training scientific support team and other relevant departments on product recalls. (2006-2011)

Managing the website scientific resources team working on protocols, troubleshooting guides, antibody guides and other documents on website technical pages. Chairing meetings. Liaising with other departments and external collaborators. Competitor analysis of competitor resource pages.(2006-2014)

Immunology Core Focus Area team - scientific and creative input into marketing material and campaigns. (2007-2011). With Marketing team.

2. Health Protection agency

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April 2003 to July 2006 Centre for Emergency Preparedness and Response Health Protection Agency, Porton Down, Salisbury, Wiltshire

General: Working to ISO9001 standards (writing and implementing SOP’s, writing and implementing risk

assessments, monitoring and records). Report writing and study plans. Laboratory management (supervision of laboratory users and training).

VARIOUS PROJECTS:

1. Anthrax research Group, Immunoassay Scientist

Using a cell based Toxin Neutralisation assay to determine the affects of various doses of a vaccine on the immune response.

Responsible for initial processing and checking of team data. Coordination of cell culture for the project. ELISA assays on the some of the same samples.

2. Special Pathogens Group, Molecular biology: Assisted with completion of SARS diagnostic development project: Restriction digests, ligation, transformation, isolation and preparation of DNA. Plasmid preps checked using PCR. Reverse transcription to RNA (including RNA extraction.

3. ECACC (European Collection of Animal Cell Cultures)

Growing Lymphoblastoid cells for DNA extraction for customers researching inherited genetic disorders such as Diabetes.

4. Scientific Services Group

The Scientific Services Department provided a flow cytometry, histology and confocal microscopy service. The department received samples from a variety of projects, and also had a variety of staff from within the organisation who received training to use the equipment for their projects.

Flow Cytometry (FC500): Development of intracellular cytokine and apoptosis assays and anthrax toxin detection assays.

Confocal microscopy:

Laboratory manager of confocal microscope suite.

Histology: General use and maintenance of usual histology equipment for processing, embedding, cutting

and staining, H and E staining TUNEL kit apoptosis staining FISH analysis

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3. Southampton UniversityLaboratory Technician / Manager (Technical grade F)May 1999 to March 2003Division of Infection Inflammation and Repair

General: Laboratory management of 6 cell culture and molecular biology laboratories. Individual training and supervision of many students (medical, PhD, postgrads) and staff. Departmental deputy safety officer and fire safety officer.

Research:Part of a research team undertaking study into epithelial cell junctions and their proteins.

Cell culture:Researching into epithelial cell junctions in the gut cell lines to establish a model for the normal state of cells. Aim: to observe effects of various factors (eg cytokines) on the expression of tight junction proteins in disease states such as inflammatory bowel disease, colitis, chrones. This included:

Cloning cells by serial dilution. Immunofluoresent staining technique (on cell cultures and frozen sections), confocal microscopy.

Primary cell culture:

Preparation of primary lung epithelial cell culture (6 well plates, coverslips, membrane inserts). Immunofluoresent staining (epithelial cell junction proteins desmoplakin, E cadherin, b catenin). Transepithelial resistance studies.

Molecular biology:

Research on mutations in the Desmocollin 2 gene promotor included:

DNA cloning, transfection techniques and analysis (mutagenesis and reporter gene assays). Vector transfected into E. Coli for maxiprep of DNA and transfected into epithelial cells. mRNA isolation from epithelial cells and RT PCR to confirm transcription of tight junction and

desmosomal genes.

4. CAMR (Centre for applied microbiology and research)Laboratory Technician (Cell culture)March 1995 to May 1999ECACC (European collection of animal cell cultures)Western blot, ELISA, cell culture (flasks, spinner flasks, bioreactors)

5. Royal Brompton Heart and Lung HospitalLaboratory TechnicianFeb 1994 to March 1995

6. Wessex Regional Blood Transfusion CentreTrainee Laboratory technician summer holiday post between A levels and degree courseJuly – Sept 1991

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Other work experience:Bar work, convenience store work and waitressing while studying.

COURSES:

Course DatesThink on your feet 6th/7th Sept 2014 Sep-14Introduction to Coaching course Apr-13Stress resilience Jul-13Excel intermediate  Situational leadership  SQL beginners Nov-11Management framework (total 5 days) 3 sessions during 2011Bioinformatics, University of Cambridge 30th Mar -1st Apr 09York University Flow cytometry practical 27th-30th Jan 09Transition to management 21-22nd Jan 09Improving personal impact and influence 11th Nov 08SiRNA 1:1 training Gurdon Institute, University of Cambridge17-18th Nov 08Communication skills 29th Aug 07Immunohistochemistry workshop (GMA embedding) Southampton

23-27th April 07

Negotiating skills 16th Jan 07Time management 10th Nov 06Cultural awareness 2nd Oct 06Business development skills 1-3rd Aug 06Supervisory Skills 20-21st Feb 2006Flow Cytometry UCL London, Dec 2005Leica Confocal Microscopy on site 1:1 training by Leica specialist

11th Oct 2005

York University Flow Cytometry theory Sept 12 – 16th 2005Statistics in biological assays 3 days Aug 2005Presentation skills 19th-20th Apr 2005GLP Laboratory Practice for Management 2 day Feb 2005Oxford University: Immunology: a path through themaze

13-12th Jan 2005

GLP Study Director 25-26th Nov 04Train the Trainer 3rd Dec 2003ECACC Cell culture 2 day, May 2003

COMPUTER SKILLS:

Familiar and competent with: Microsoft Word, PowerPoint, Excel, Outlook Photoshop Various data analysis software

LEISURE TIME:

Reading, photography, dancing, walking, travel

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PUBLICATIONS AND ABSTRACTS:

Adaption of BHK Cells Producing A Recombinant Protein To Serum Free And Protein Free Medium.Cytotechnology (1997), 26: 59-64H.J. Cruz, J.L. Moreira, G.Stacey, E.M. Dias, K. Hayes, D. Looby, J.B Griffiths, M.J.T Corrondo

The Production Of Influenza Virus By Immobilised MDCK Cells.Presented at 15th European Society of Animal Cell Technology. Tours, France. 7-11th Sept 1997 (Awarded poster prize, second place)D. Looby, J.A Tree, A. Talukder, K. Hayes, H. House, G.N Stacey

The Production Of Influenza Virus By Immobilised MDCK Cells.New Developments and New Applications in Animal Cell Technology 1998 (eds)O-W Merten et al p 551 – 554 D. Looby, J.A Tree, A. Talukder, K. Hayes, H. House, G.N Stacey

The Effects Of Immobilisation On The Metabolism, Productivity And Product Quality of Recombinant BHK CellsPresented at Cell Engineering VI meeting 1998, Feb 7-12, San Diego, USAD. Looby, K. Hayes, H. House, G. Stacey

An Integrated Strategy For The Process Development Of A Recombinant Antibody – Cytokine Fusion Protein Expressed In BHK Cells.Applied Microbiology and Biotechnology (1999), 52: 345-353C. Burger, M.J.T Carrondo, H.Cruz, M Cuffe, E.Dias, J.B.Griffiths, K.Hayes, H.Hauser, D.Looby, C.Mielke, J.L.Moreira, E.Rieke, A.V.Savage, G.N.Stacey, T.Welge

The Release Of Alpha1-Antitrypsin and Alpha1-Antichymotrypsin From Human Bronchial Epithelial Cells: The Effect of ElastasePoster Presentation at the American Thoracic Society Annual Meeting USA 2000H. Sharma, R. C. Foreman, , A. D. B. Furr, K. L. Hayes, W. R. Roche, J. E. Collins, J. A. Warner

The release of 1-antitrypsin and 1-antichymotrypsin from human bronchial epithelial cells.Poster Presentation at the American Thoracic Society Annual Meeting Boston USA 2001RC Foreman, JA Warner, ADB Furr, H Sharma, KL Hayes, BJ Addis, WR Roche and JE Collins.

Inflammatory processes have differential effects on claudins 2,3 and 4 in colonic epithelial cells.Laboratory Investigation (2005), 85, 1139-1162Shyam Prasad, Roberto Mingrino, Katri Kaukinen, Katherine L Hayes, Robert M Powell, Thomas T MacDonald, Jane E Collins

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