kit for the titration of anti-streptodornase b antibodies ......2. distribution of serum into the...
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DOR-BARG r o u p A S t r e p t o c o c c u S S e r o d i A G n o S i S
Kit for the titration of anti-streptodornase BantiBodies (asdor)
PerformantSimple and convenientVisual and colorimetric results
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2. Distribution of serum into the tray
1. Dilution of serum
3. Regeneration of substrat 4. Distribution
5. IncubationIncubate 4 hours at 37°C
6. Results
DOR-BAR
DIL
50 µl of serum dilution (S) 50 µl D 50 µl S
50 µl of generated SUBS
Titer: 150 U/ml
Titer: 400 U/ml
Colour change to pink
Absence of colour change
+
Shake gently during 1 minute to mix
600 µlDOR-BAR
SUBS
Dispense 50 µL of the serum dilution into each of the wells (except C-)
Dilute the serum sample(1/80) with the diluant :10 µLof serum 790 µLof DOR-BAR DIL
Transfer 600 µl of H2O into SUBS of substrat
“Test”wells “Control”wells
DOR-BARpa
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M e t H o d o L o G y :
REFERENCE NAME QTY
04135 DOR-BAR coffret de 12 tests
c A t A L o G u e :
p r o d u c t S :
DIL : 5 ml vial of diluant.SUBS : Vial of lyophilized medium containing the substrate (dnA) and a colour indicator. to be reconstituted with 600 μl of sterile distilled water.
DOR-BAR tray : divisible tray of 2 x 10 wells (2 tests), individually wrapped in aluminium pouches. the trays contain increasing concentrations of dehydrated streptodornase B.
Control Serum: 1 vial of 2.2mL positive control, standard at 600 u/mL (human serum).
B e n e f i t S :
Convenient and Cost-effeCtive
◉ no instrument required
◉ Space-saving trays for the incubator
easy to use - fast
◉ Simple methodology
◉ only one incubation step (4h at 37°c)
◉ Visual and colorimetric results
safe results
◉ negative control c- (well 9 containing streptodornase B but no serum)
◉ positive control c+ (well 10 containing serum but no streptodornase B)
◉ Standard control serum (Human serum in a separate vial)
Take the diagnosis further with the ASO-BAR kit(Ref. 04125)◉ titration of anti-streptolysine o antibodies (ASLo)
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