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Kloning av DNA fragment in i E.coli

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Page 1: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC

Kloning av DNA fragment in i E.coli

Page 2: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 3: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 4: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 5: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 6: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 7: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 8: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 9: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 10: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 11: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 12: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC

dnaconda.riken.jp2.0 Erectrophoresis 100 Restriction enzyme Noti Xhol Noti+Xhol O z o X 1 x TAE Buffer kbp kbp kbp kbp 19 nanogram DNA per lane ; 1% agarose gel , Expected size of

06_20_cancer_age.jpg. Gene Mutation: Origins and Repair Processes GAATTC GTATTC A a Skin cancers from Xeroderma pigmentosum, a recessive hereditary

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Dieses Dokument wurde von einer Papierkopie gescannt und ... · BMT/3/2 page 5 1 8 9 enzymes have been discovered, for example EcoRI recognises and cuts the sequence GAATTC. Figure

CHAPTER 8: RECOMBINANT DNA TECHNOLOGY · EcoRI 5’-GAATTC-3’ 3’-CTTAAG-5’ BamHI 5’-GGATCC-3’ 3’-CCTAGG-5’ SmaI 5’-GGGCCC-3’ 3’-CCCGGG-5’ The list and explanation

Phagedisplayofricin Bchainandits single bindingdomains ...SBD1 Pst1i-j SBD2 46 N'1 T143-/DFXhoI N2655 F262 1-XhoI T143 100 bp F Xhol 26 262 255 F N FIG. 1. Structure ofgene 3 (A) and

Genetic Engineering. Restriction Enzymes Cut DNA sequences at specific repeating patterns. EcoRI cuts at GAATTC which will appear at different points

GAATTC AATGGCATAC-3’ TTACCGTATC-5’ - Babec · functions of the genes and occur ... contribute a hair for DNA extraction. ... Change tip every time the pipette is used even if

Selective Protection of 5' . . . GGCC . 3' and GCNGC . 3' Sequences

Road Map Analogy of Molecular Mapping - NDSU · Classes of Molecular Markers ... Restriction enzyme = EcoRI cuts between the G and A. in the sequence GAATTC Methylase = EcoRI methylase

Growth Hormone (GH)mcb.berkeley.edu/courses/mcb41/lecture_17.pdfReview of recombinant DNA technology Growth Hormone Reading for this and next lecture: Chapter 19 GAATTC CTTAAG G CTTAA

25 February, 2005 Chapter 10 Gene Mutation: Origins and Repair Processes GAATTC GTATTC A a

Analysis of the yeast SPT3 gene and Identification of its ...-winston-1986.pdf · Xhol fragment in pBR322 (29)( for Ty, B161, carrying a Ty Bglll restriction fragment in pBR322 (R

The Carbon budgets that will prevent the average …...Technology and Humanism. Under this "historic and beloved umbrella" a subgroup was set up: the GGCC – Group on the Governance

A Novel Expression Vector for the Cyanobacterium ......182 Expression Vector for Synechococcus PCC 6301 [Vol. 1, a. pBA1 (7.8 kb) b. pBR322M (4.3 kb) Hindlll Bglll Hpal Pvull Xhol

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Green Business In Illinois GGCC... · What is the Illinois Green Business Association? •501(c)(3) Non-Profit Organization •Mission: help Illinois businesses realize the economic

Effectof enterotoxin 1 in - Gut · Fasano, Noriega, Liao, Wang, Levine Xmnl Scal 2526- Xmnl Scal 3618. T7 promoter Kpal Apal Xhol Sall (Hincil) Accl Hindill EcoRV EcoRI-Pstl Smal

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Palindrome - 國立臺灣大學juang.bst.ntu.edu.tw/files BC/BC2007/NA(1) 2007A.pdf · 2016. 2. 16. · Palindrome, Restriction Enzyme, Sticky Ends GAATTC GAATTC G AATTC G AATTC Sticky

THE GGCC QUARTERLY NEWSLETTER VOLUME VIII ......committee structure to make sure our efforts are aligned with our goals. We welcome your input. If you have ... customer loyalty, team