l. viÑas, m.a. franco, j.a. soriano and j.j. gonzÁlez
DESCRIPTION
Spatial and temporal distribution of petroleum hydrocarbons in wild mussels in Galicia after the Prestige oil spill. L. VIÑAS, M.A. FRANCO, J.A. SORIANO and J.J. GONZÁLEZ Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, Cabo Estai-Canido. 36200 Vigo, Spain - PowerPoint PPT PresentationTRANSCRIPT
Spatial and temporal distribution of petroleum hydrocarbons in wild
mussels in Galicia after the Prestige oil spill
L. VIÑAS, M.A. FRANCO, J.A. SORIANO and J.J. GONZÁLEZ Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, Cabo Estai-Canido. 36200 Vigo, Spain
L. ORTIZ, J.M. BAYONA and J. ALBAIGÉS Department of Environmental Chemistry, CID-CSIC, Jordi Girona, 18-26, 08034-Barcelona, Spain.
Mussel as a sentinel organism:
• Concentrate substances over their levels in the environment.
• The tissue reflects environmental changes.
• Stay in a single location: Sessile.
• Fairly resistant to chemical contamination.
• Abundant along the studied area.
Sampling
• 24 stations
• October 2000 pre-spill
• February 2003 3 months after spill
• June 2003 7 months after spill
Ribadeo
ViveiroEspasante
Cedeira
Ferro l-P íasFerro l-Palm a
Coruña-M era
Ares
La G uardiaS ta.M ª O ia
V igo-Sam il
V igo-La G uíaCabo Hom e
Pontevedra-Raxó
Pontevedra-Loira
Corrubedo Arosa
M uros-Freixo
M uros-S .Anton
Pta. Insua
Fin isterre
M uxía
Caion
Corm e
Coruña-T.Contro l
Analytes determined
• Aliphatic hydrocarbons, with special emphasis on the profiles of fossil markers, such as triterpanes and steranes.
• Analysed by GC-MS
Analytes determined
• Aromatic hydrocarbons, 13 parent PAHs.
• Analysed by HPLC-FLD
Fenantreno Antraceno Fluoranteno Pireno Benzo[a]Antraceno
Criseno Benzo[e]Pireno Benzo[b]Fluoranteno Benzo[k]Fluoranteno
Benzo[a]Pireno Dibenzo[a,h]Antraceno Benzo[g,h,i]Perileno Indeno[1,2,3-c,d]Pireno
General distribution of PAHs
0
500
1000
1500
2000
2500
3000
3500
4000
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
stations
ng
/g d
w
2000
2003 Febr.
2003 Junio
October 2000-February 2003
0100
200300
400500
600700
800
Su
m 1
3 P
AH
s (µg
/kg
p.s
)
oct-00 feb-03
0100020003000400050006000700080009000
Su
m 1
3 P
AH
s (µg
/kg
p.s
)
oct-00 feb-03
0100200
300400500600
700800900
Su
m 1
3 P
AH
s (µ
g/k
g p
.s)
oct-00 feb-03
R ibadeo
ViveiroEspasante
Cedeira
Ferro l-P íasFerro l-Palm a
Coruña-M era
Ares
La G uardia
S ta.M ª O ia
V igo-Sam il
V igo-La G uíaCabo Hom e
Pontevedra-Raxó
Pontevedra-Loira
CorrubedoArosa
M uros-Freixo
M uros-S .Anton
Pta. Insua
Fin isterre
M uxía
Caion
Corm e
Coruña-T.Contro l
October 2000-February 2003-June 2003
0
100200
300
400
500600
700
800
Su
m 1
3 P
AH
s (µ
g/k
g p
.s)
oct-00 feb-03 jun-03
0100020003000400050006000700080009000
Su
m 1
3 P
AH
s (µ
g/k
g p
.s)
oct-00 feb-03 jun-03
0100200300400
500600700800900
Su
m 1
3 P
AH
s (µg
/kg
p.s
)
oct-00 feb-03 jun-03
R ibadeo
ViveiroEspasante
Cedeira
Ferro l-P íasFerro l-Palm a
Coruña-M era
Ares
La G uardia
S ta.M ª O ia
V igo-Sam il
V igo-La G uíaCabo Hom e
Pontevedra-Raxó
Pontevedra-Loira
CorrubedoArosa
M uros-Freixo
M uros-S .Anton
Pta. Insua
Fin isterre
M uxía
Caion
Corm e
Coruña-T.Contro l
PAH profile
0
10
20
30Phe
Ant
Fluo
Pyr
BaA
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
MUXÍA
010
2030
40Phe
Ant
Fluo
Pyr
BaA
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
010
2030
40Phe
Ant
Fluo
Pyr
BaA
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
0102030
40Phe
Ant
Fluo
Pyr
BaA
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
0102030
40Phe
Ant
Fluo
Pyr
BaA
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
BghiP
PUNTA INSUA
0
10
20
30Phe
Ant
Fluo
Pyr
BaA
Chry
BePBbF
BkF
BaP
dBahA
In123cdP
0
10
20
30
40Phe
Ant
Fluo
Pyr
BaA
Chry
BePBbF
BkF
BaP
BghiP
dBahA
In123cdP
0
10
20
30
40Phe
Ant
Fluo
Pyr
BaA
Chry
BePBbF
BkF
BaP
BghiP
dBahA
In123cdP
0100020003000400050006000700080009000
Sum
13
PA
Hs
(µg/
kg p
.s)
oct-00 feb-03 jun-03
PAH profile
0
10
20
30Phe
Ant
Fluo
Pyr
BaA
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
CABO HOME
Pyr
BaA
Pyr
BaA
01020
30
40Phe
Ant
Fluo
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
01020
30
40Phe
Ant
Fluo
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
0
1020
30
40Phe
Ant
Fluo
Pyr
BaA
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
0
1020
30
40Phe
Ant
Fluo
Pyr
BaA
ChryBePBbF
BkF
BaP
BghiP
dBahA
In123cdP
FERROL-PÍAS
0
10
20
30Phe
Ant
Fluo
Pyr
BaA
Chry
BePBbF
BkF
BaP
BghiP
dBahA
In123cdP
0
10
20
30Phe
Ant
Fluo
Pyr
BaA
Chry
BePBbF
BkF
BaP
BghiP
dBahA
In123cdP
0
10
20
30Phe
Ant
Fluo
Pyr
BaA
Chry
BePBbF
BkF
BaP
BghiP
dBahA
In123cdP
0
100200
300
400
500600
700
800
Su
m 1
3 P
AH
s (µg
/kg
p.s
)
oct-00 feb-03 jun-03
0100200300400
500600700800900
Su
m 1
3 P
AH
s (µg
/kg
p.s
)
oct-00 feb-03 jun-03
Isomeric ratios
27Ts: 18(H)-22,29,30-trisnorneohopane/17(H)-22,29,30-trisnorhopane %29ab: 17(H),21(H)-30-norhopane/17(H),21(H)-hopane %29aaS: 24-ethyl-14(H),17(H)cholestane (20S/20R) %29bb: 24-ethyl-14(H),17(H)cholestane (R+S)/24-ethyl-14(H),17(H)cholestane(R+S)
0
10
20
30
40
50
60
%27Ts %29ab %29aaS %29bb
Fuel 4 9 1213 14 20 24
Conclusions
• Two main groups of mussels: affected and not affected by the spill
• Increase of hydrocarbons in February and subsequent decrease in June.