Quality and Authentication of Products Unit (QAP Unit) 1

La spectroscopie proche infrarouge au CRA-W

(non exhaustif …)

Vincent Baeten, Philippe Vermeulen, Audrey Pissard, Abigaël Anselmo, Olivier

Minet, Bernard Lecler, Damien Vincke, Juan Antonio Fernández Pierna

v.baeten@cra.wallonie.be

Quality and Authentication of Products Unit

Walloon Agricultural Research Centre

Gembloux, Belgium

Quality and Authentication of Products Unit (QAP Unit) 2

Quality and

Authentication of

Products Unit

(Unit QAP)

Walloon Agricultural

Research Centre

Dr Vincent Baeten

Scientific Director Unit QAP

v.baeten@cra.wallonie.be

« An active and innovative

Unit involved in the

development of sustainable

methods to reinforce and

assess the quality and

authentication of

agricultural and agro-food

products »

Quality and Authentication of Products Unit (QAP Unit) 3

Near-InfraredSpectrocopyLaboratory

Bernard Lecler/Audrey

Pissard

(NIR)

MolecularBiology

Laboratory

Olivier Fumière

(BIOMOL)

MicrobiologyLaboratory

Véronique Ninane

(µBIO)

On-field and Process control

Laboratory

Philippe Vermeulen

(FIELDPRO)

Data, Raman, Imaging & MIR Spectroscopy

Laboratory

Juan A. Fernández

Pierna (DRIM)

Optical and Digital

MicroscopyLaboratory

Pascal Veys

(µSCOP)

QAP Unit

30 staff members committed

to develop and to validate,

analytical methods and tools

Chemometrics

&

Bio-informatic

Proteomic

(MS)

Quality and Authentication of Products Unit (QAP Unit) 4

Near-InfraredSpectrocopy

Lab.

Bernard Lecler/Audrey

Pissard

(NIR) 1989 - …

MolecularBiology

Laboratory

Olivier Fumière

(BIOMOL)

MicrobiologyLaboratory

Véronique Ninane

(µBIO)

On-field and Process control

Lab.

Philippe Vermeulen

(FIELDPRO)

2017 - …

Data, Raman, Imaging & MIR Spectro. Lab.

Juan A. Fernández

Pierna (DRIM)

2001 - …

Optical and Digital

MicroscopyLaboratory

Pascal Veys

(µSCOP)

QAP Unit

30 staff members committed

to develop and to validate,

analytical methods and tools

Chemometrics

&

Bio-informatic

Proteomic

(MS)

Quality and Authentication of Products Unit (QAP Unit) 5

2001

1997

2007

2008

NIR benchtop, on-line, microscope

Hyperspectral

NIR Imaging

Baby NIR

MIR

Raman

1989

2021

Quality and Authentication of Products Unit (QAP Unit) 6

EXPERTISES

CalibrationInstrument

network

ISO17025

&

ISO17043

Interlaboratorystudy

Training

Spectral band interpretation

Homogeneitystudy

Chemometrics

Sampling

Samplepresentation

Standardisation

Data bases

Quality and

authentication of

products Unit

Quality and Authentication of Products Unit (QAP Unit) 7

NIR vsProducts

Oil

Fruit

Feaces

Soil

Bio-energy

Bio-transformation

Phytosanitaryproducts

Plant products

Feed

Forage

PAP

Milk

Meat

Cereals

Frédéric Dehareng

Clément Grelet

U11

Virginie Decruyenaere

U5

Amélie Vanlierde U11

Virginie D. U5

Georges Sinnaeve

Sébastien Gofflot

U11

Bruno Godin

U11

Marc Lateur

U5

Donatienne Arlotti

Bruno

Huyghebaert

U7

Olivier Pigeon

U10

CRA-W

internal

collaboration

Quality and Authentication of Products Unit (QAP Unit) 8

Analysis in the

fields and orchards

PHENWHEAT

Cereal phenotyping

BEETPHEN

Sugar beet

phenotyping

FIRST

Potato phenotyping

INVITE

Evaluation of

varieties in Europe

Analysis in the farm

and in the industry

EFFORT

Lab Farm

INDIGGES

GHG

ECOAFRICA

IAEA-Feces

Efficiency of tropical

forages

Analysis in the

laboratory

EURL-AP *

European Laboratory

for Animal protein

FARMYNG

Insect meal

ANTAGONIST

Microbiology

identification

WBI-MOST

Contaminants

And Impurities

Analysis in the

Laboratory

LNR-OGM

LNR-Feed Additives

IAEA-Handheld

Baby-NIR

BELCOBRA

Cocoa and Chocolate

DSM

NIR expertise

REQUASUD

NIR network

CADDY-NIR

Food

FERTIWALIM

Bovine Fertility

TERA4ALL - Thz

POLBEES - Bee

Quality and Authentication of Products Unit (QAP Unit) 9

From laboratory to orchards

Research of rapid methods

to select new apple cultivars

presenting high nutritional quality

https://www.h2020-invite.eu/ 2019-2024

From laboratory to field

Quality and Authentication of Products Unit (QAP Unit) 10

PhenWheat : A field phenotyping

platform for wheat trials

Quality and Authentication of Products Unit (QAP Unit) 11

Transition of dairy farming toward a better

efficient use of fodder resources

Sampling of corn silage Sampling of haylage bale

Corn silage (April 12 2018), red points on silo front representsampling points.

Haylage bale, red points represent sampling points.

Quality and Authentication of Products Unit (QAP Unit) 12

Base de données spectrales

Développement d’une base de données (Protospec) avec 47,393 spectres NIR importés depuis avril 2020

Facilité de recherche de spectres/appareil/produit/DQ(série d’échantillon)

Recherche de spectres IR par projet

Recherche de spectres IR par appareil et

par produit

Quality and Authentication of Products Unit (QAP Unit) 13

Status 2020

The CRAWLSPEC initiative : Spectra Data Base System Project

Quality and Authentication of Products Unit (QAP Unit) 14

Quality and Authentication of Products Unit (QAP Unit) 15

Web-based tools for managing spectral data-bases

Training boxes

Protocols

Quality and Authentication of Products Unit (QAP Unit) 16

2021

…

To benefit from HSI

advantages for high

throughput analysis : to go

to batch segmentation

Need of traceability and

authentication tools

Cruz-Tirado, J.P. , Fernandez Pierna, J.A. , Rogez, H. , Fernandes Barbin, D.

& Baeten, V. (2020). Authentication of cocoa (Theobroma cacao)

bean hybrids by NIR-hyperspectral imaging and

chemometrics. Elsevier : Food Control, 118 : 107445: 1-13.

BelCobra project : WBI/CAPES

Quality and Authentication of Products Unit (QAP Unit) 17

Chemometrics– what’s new?

Rapid & specific regression methods

Local Calibration by Percentile Selection

(LCPS)

Local Calibration by Customized Radii

Selection (LCCRS)

Local PLS based on global PLS

scores

Quality and Authentication of Products Unit (QAP Unit) 18

Chemometrics– what’s new?

Local regression modelling

Quality and Authentication of Products Unit (QAP Unit) 19

Chemometrics– what’s new?

15% RMSEP

decrease

12.5%

RMSEP

decrease

14% RMSEP

decrease

12.5%

RMSEP

decrease

Moisture

Protein

Local regression modelling

Quality and Authentication of Products Unit (QAP Unit) 20

Targeted detectionUntargeted detection

Target versus untarget

Source : Sørensen et al., The use of rapid spectroscopic

screening methods to detect adulteration of food raw

materials and ingredients, Current Opinion in Food

Science. (2016). DOI: 10.1016/j.cofs.2016.08.001

Quality and Authentication of Products Unit (QAP Unit) 21

Local Window Principal Component Analysis (LWPCA)

UNTARGETED ANALYSIS

One Class modelling

Regressionequations

Local

Windows PCA

GH similarities

Quality and Authentication of Products Unit (QAP Unit) 22

U12 staff involved in spectroscopy and chemometric

: A team at your service

Audrey Pissard Juan A. Fernández Pierna

Philippe Vermeulen Sandrine Mauro

Damien Vincke Nicolas Crasset

Abigaël Anselmo Lisa Plasman

Benoit Scaut Quentin Arnould

Maxime Joissains Nicaise Kayoka Mukendi

Stéphane Brichard Vincent Baeten

Olivier Minet

With the strong support of Colleagues and Retired Young Seniors

Georges Sinnaeve, Sébastien Gofflot, Virginie Decruyenaere,

Bruno Godin, Donatienne Arlotti, Clément Grelet, …, Amélie

Vanlierde, Marc Lateur, Bernard Lecler & Pierre Dardenne

Quality and Authentication of Products Unit (QAP Unit) 23

https://www.pinterest.fr/pin/72128031508013467/