lab 19 –pituitary gland pituitary glandlab 19 –pituitary gland a560 –fall 2015 i. introduction...
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![Page 1: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/1.jpg)
PituitaryGlandLab19– PituitaryGland
A560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. SummaryFig20‐2,Junqueira,13th ed.
![Page 2: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/2.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide128:Pituitary,H&E
anteriorpituitary
posteriorpituitary
parsintermedia
![Page 3: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/3.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide128(Hi‐Res):Pituitary,H&E
anteriorpituitary
posteriorpituitary
parsintermediaw/remnantsofRathke’s pouch
![Page 4: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/4.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide103:AnteriorPituitary,H&E
anteriorpituitary
posteriorpituitary
parsintermedia
![Page 5: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/5.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide122:Pituitary,H&E
anteriorpituitary
posteriorpituitary
parsintermedia
![Page 6: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/6.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide74:Pituitary,GomoriTrichrome
anteriorpituitary
posteriorpituitary
![Page 7: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/7.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide128(Hi‐Res):Pituitary,H&E
anteriorpituitary
posteriorpituitarypars
intermedia
parsdistalis
parstuberalis
![Page 8: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/8.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide122:Pituitary,H&E
the pars distalis accounts for 75% of the anterior pituitary (adenohypophysis); it is composed primarily ofcords of endocrine cells interspersed with fenestrated capillaries; some reticular CT is also present
![Page 9: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/9.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide128(Hi‐Res):Pituitary,H&E
anteriorpituitary
posteriorpituitary
parsintermedia
the pars intermedia contains basophilic cells between the pars distalis of the anterior pituitary and thepars nervosa of the posterior pituitary; eosinophilic colloid‐filled cysts represent remnants of thehypophyseal pouch (Rathke’s pouch); functional significance of this region is uncertain
![Page 10: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/10.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide74:Pituitary,GomoriTrichrome
anteriorpituitary
posteriorpituitary
Lookhereforsecretorycells
![Page 11: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/11.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide74:Pituitary,Gomori Trichrome
chromophobe
acidophil
basophil
![Page 12: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/12.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide122:Pituitary,H&E
chromophobes are identified by their pale‐staining cytoplasm and absence ofsecretory granules; they represent several different types of cells, including stem cellsand degranulated cells
acidophil basophil
![Page 13: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/13.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide74:Pituitary,Gomori Trichrome
acidophils have strongly‐staining acidophilic (eosinophilic) cytoplasm; they secrete growthhormone and prolactin (somatotrophs [about half of total cells] and lactotrophs, respectively)
![Page 14: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/14.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide74:Pituitary,Gomori Trichrome
basophils (less numerous) have strongly‐staining basophilic cytoplasm; they include:corticotrophs (ACTH and LPH), gonadotrophs (FSH and LH/ICSH), and thyrotrophs (TSH)
![Page 15: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/15.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide128(Hi‐Res):Pituitary,H&E
anteriorpituitary
posteriorpituitary
parsnervosa
infundibulumstalk
![Page 16: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/16.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
Slide128(Hi‐Res):Pituitary,H&E
pituicytes, majority of nuclei seen, are glial cells that support the unmyelinated axons that compose majorityof posterior pituitary (neurohypophysis); axons release ADH and oxytocin (nothing produced in posteriorpituitary); Herring bodies, faintly‐staining eosinophilic accumulations of secretory products in axon terminals(not to be confused with capillaries), might be seen
![Page 17: Lab 19 –Pituitary Gland Pituitary GlandLab 19 –Pituitary Gland A560 –Fall 2015 I. Introduction II. Learning Objectives III. Slides and Micrographs A.General structure B.Parts](https://reader030.vdocument.in/reader030/viewer/2022040811/5e539f83568e46615d3bdd27/html5/thumbnails/17.jpg)
Lab19– PituitaryGlandA560– Fall2015
I. IntroductionII. LearningObjectivesIII. SlidesandMicrographs
A. GeneralstructureB. Parts1. AnteriorPituitarya. Divisionsi. Parsdistalisii. Parsintermediaiii. Parstuberalis
b. Cellsi. Chromophobesii. Chromophils1) Acidophils2) Basophils
2. PosteriorPituitarya. Divisionsi. Infundibulumstalkii. Parsnervosa
b. Cellsi. Pituicytes
IV. Summary
EMstoExamine
Fig20‐7:Ultrastructureofsomatotropic cells