lab exercise 17:
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Lab Exercise 17:. Biochemical Differentiation of some Medically Important Gram-negative Bacilli. MacConkey Agar. Left: Shigella - result Right: Escherichia + result. Carbohydrate Fermentation Tests. Glucose w/ Durham Fermentation Tube. Carbohydrate Fermentation Tests. - PowerPoint PPT PresentationTRANSCRIPT
Lab Exercise 17:
Biochemical Differentiation of some Medically Important Gram-negative
Bacilli
MacConkey Agar
Left: Shigella
- result
Right: Escherichia
+ result
Carbohydrate Fermentation TestsGlucose w/ Durham Fermentation Tube
Carbohydrate Fermentation TestsSucrose Fermentation
Triple Sugar Iron
-/+/+
Indole Test
broth with tryptophan
IMViC Series
Methyl Red TestIMViC Series
Voges-Proskauer Test
Leave uncapped for 15-20 minutes……..
IMViC Series
MRVP broth
Citrate Utilization Test
- +
IMViC Series
Urease Test
Slow positive
Negative result (acidic)
Uninoculated urea agar
Positive result (alkaline)
Motility Test
MacConkey agar
1. Which plate could have E. coli on it?
2. Which plate shows the presence of a lactose-fermenter?
3. Which plate could have Salmonella on it?
4. Which plate shows the presence of a
lactose non-fermenter?
Answers:
1.A3. B
2. A4. B
A
B
What is the name of the lab procedure demonstrated here?
Answer: Stabbing the butt!
Use the chart on p. 181 and or the flow chart on p. 182 of your lab manual to figure out who I am!
I M V
C
U
Answer: Shigella sonnei
I M V
C
U
Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
Answer: Escherichia coli
I M V C
U
Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
Answer: Serratia marcesens
Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
I M V C
Answer: Klebsiella pneumoniae
Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
I M V C U
Answer: Salmonella typhimurium
Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
I M VC
U
Answer: Proteus vulgaris
Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
I M V C
Answer: Enterobacter aerogenes
U