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Labgard Class II, Type A2 Laminar Flow Biological Safety Cabinet

Models

NU-602-4XX/5XX/6XX NU-602-4XXE/5XXE/6XXE

Console

Operation & Maintenance Manual

October, 2011 (Series 25)

Revision 3

Manufactured By: NuAire, Inc.

2100 Fernbrook Lane Plymouth, MN 55447

Toll-Free: 1-800-328-3352 In Minnesota: (763)-553-1270

Fax: (763)-553-0459

115 VAC Only 115 VAC Only

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Congratulations!

You have just purchased one of the finest Laminar Flow Biological Safety Cabinets available. With proper care, maintenance (certification), and laboratory procedure, this cabinet will give you years of product and personnel protection from particulate contaminants as prescribed in National Sanitation Foundation (NSF) Standard No. 49. Please read this manual carefully to familiarize you with proper installation, maintenance and operation of the cabinet. Other reference and guideline materials are available through the following web sites.

www.hc-sc.gc.ca www.cdc.gov/od/ohs/ www.absa.org www.absa-canada.org www.ebsa.be www.inspection.gc.ca www.who.int www.biosafety.be www.hse.gov.uk www.nsf.org www.cetainternational.org www.osha.gov/dts/osta/ www.nuaire.com

Acknowledgment NuAire, Inc. acknowledges that some material in this manual reflects information supplied by the National Institutes of Health personnel both in verbal and written specifications. In particular, NuAire acknowledges that information in Section 8 was obtained from the following sources: 1. Technical Report No. FPS 56500000001. Prepared by Dow Chemical Co., for the National Cancer Institute, May 1, 1972. 2. Stolar MH, Power LA, Vielo CS: Recommendations for handling cytotoxic drugs in hospitals. Am J Hosp Pharm 1983;40: 1163-1171. 3. Anderson R.W., Director of Pharmacy, University of Texas, M.D. Anderson Hospital and Tumar Institute at Houston.

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ABOUT THIS OPERATION & MAINTENANCE MANUAL

The information contained in this manual is intended to reflect our current production standard configuration model along with the more frequently purchased options. Any unique additions, modifications, shop drawings are appended in the back flap of this manual, along with any modifications and/or additions to procedures as outlined in this manual. A copy of the original factory test report is also appended to this manual. In case this manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A replacement copy can be obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief description of the information desired.

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Labgard Class II, Type A2 Laminar Flow Biological Safety Cabinet

Models NU-602-4XX/5XX/6XX NU-602-4XXE/5XXE/6XXE

Operation & Maintenance Manual

TABLE OF CONTENTS Section No. 1 ...................................................................... General Description Section No. 2 ...................................................................... Models and Features 2.1 ................................................................... Optional Systems Section No. 3 ...................................................................... Warranty Section No. 4 ...................................................................... Shipments Section No. 5 ...................................................................... Installation Instructions

5.1 ....................................................................... Location 5.2 ....................................................................... Set-up Instructions 5.3 ....................................................................... Certification Testing Methods and Equipment Section No. 6 ...................................................................... Operating the NU-602 6.1 ....................................................................... Operator Controls & Indicators 6.2 ....................................................................... Auto-Lift Operating Controls 6.3 ....................................................................... Operating Guidelines 6.4 ....................................................................... Ergonomics 6.5 ....................................................................... Operating Sequence 6.6 ....................................................................... Cleaning Procedure Section No. 7 ...................................................................... General Maintenance 7.1 ....................................................................... Decontamination 7.2 ....................................................................... Fluorescent Lamp Bulb Replacement 7.3 ....................................................................... HEPA Filter/Motor Replacement 7.4 ....................................................................... Auto-Lift System 7.5 ....................................................................... Airflow Calibration 7.6 ....................................................................... Filter Integrity Check 7.7 ....................................................................... Cabinet Leak Tightness Test Section No. 8 ...................................................................... Error Indicators & Troubleshooting Section No. 9 ...................................................................... Remote Contacts Section No. 10 .................................................................... Optional Equipment 10.1 ..................................................................... Ultraviolet Light Section No. 11 .................................................................... Electrical/Environmental Requirements Section No. 12 .................................................................... Disposal and Recycle Insert ....................................................................................... Replacement Parts List

MANUAL DRAWINGS BCD-05658 ................................................... NU-602 Airflow Schematic BCD-05436 ................................................... NU-602-400/400E BCD-05437 ................................................... NU-602-500/500E BCD-05438 ................................................... NU-602-600/600E BCD-05440 ................................................... NU-602 w/Sterile Cage Delivery Option BCD-05441 ................................................... NU-602 w/Dirty Cage Collection Option BCD-05439 ................................................... NU-602 w/Refuse Disposal Option BCD-01816 ................................................... Service Control Center Installation BCD-06719 ................................................... Control Panel

ASSEMBLY DRAWINGS ACD-04275 ................................................... Exhaust Filter Guard Installation BCD-00919 ................................................... Filter Replacement Procedure BCD-05457 ................................................... Auto-Lift Castor Base Assembly

ELECTRICAL DRAWINGS

BCD-13237 ................................................... NU-602-400/500/600 Electrical Schematic BCD-13238 ................................................... NU-602-400E/500E/600E Electrical Schematic

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Labgard Class II, Type A2 Laminar Flow Biological Safety Cabinet

Animal Cage Changing Station Models

NU-602-4XX/5XX/6XX NU-602-4XXE/5XXE/6XXE

Manufactured By:

NuAire, Inc. - Plymouth, Minnesota

1.0 General Description The LABGARD Model NU-602 Laminar Flow Biological Safety Cabinet (LFBSC) is highly similar to the NuAire Model NU-407 but slightly modified to better serve the personnel in animal research. The NU-602 has passed the performance criteria of NSF 49 in tests conducted at NuAire, Inc. in Plymouth, MN. The Laminar Flow Biological Safety Cabinet, (LFBSC) when used with proper technique, is an effective laboratory aid in obtaining the optimum control over product quality while reducing the potential for exposure of both animal and personnel to airborne biological or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product operations, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia.

In order to meet the special needs of the science of animal research, the NU-602 includes the following significant features:

1. Greater visibility because the fluorescent lamp lighting the interior of the cabinet is placed

inside the back of the work zone. A shield prevents direct viewing of the lamp. Thus, the full window is clear for viewing the work area.

2. Larger work opening access under the viewing window to the work zone is increased to 12 inches (305mm).

3. Autolift System with heavy duty stainless steel castors have been integrated into the base, yet keeping the overall cabinet height at 77.50 (1968mm) inches. At this height, the cabinet can be rolled through most doorways. The cabinet is very easy to move and rise to variable working heights for user ease.

4. Prefilter is placed ahead of the HEPA filters to remove hair and dander, prolonging HEPA life. The prefilter is easy to service.

In addition, to the foregoing, three significant systems are offered as purchasable options which greatly improve work flow, increase flexibility and improve cleanliness in the handling of the animals:

• Sterile Cage Delivery Option • Dirty Cage Collection Option • Refuse Disposal Option

The NU-602 console LFBSC is known as a Class II, type A2 Biological Safety Cabinet. This is possible since NuAire's cabinet conforms to the following requirements:

1. Maintains a minimum average inflow velocity of 100 LFPM through the work access opening. 2. Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust

plenum. 3. Exhaust airflow can either be room re-circulated or exhausted outside using a canopy exhaust

transition. 4. Has all biologically contaminated ducts and plenums under negative pressure or surrounded by

negative pressure (type A1 permits positive pressure contaminated ducts and plenums).

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1.2 Safety InstructionsThese safety instructions describe the safety features of the LABGARD Model NU-602 LFBSC.The safety cabinet has been manufactured using the latest technological developments and has been thoroughly tested before delivery. It may, however, present potential hazards if it is not used according to the intended purpose or outside of operating parameters. Therefore, the following procedures must always be observed:

• The safety cabinet must be operated only by trained and authorized personnel.• For any operation of this unit, the operator must prepare clear and concise written instructions

for operating and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in particular.

o which decontamination measures are to be applied for the cabinet and accessorieso which protective measures apply while specific agents are usedo which measures are to be taken in the case of an accident

• Repairs to the device must be carried out only by trained and authorized expert personnel.• Keep these operating instructions close to the unit so that safety instructions and important

information are always accessible.• Should you encounter problems that are not detailed adequately in the operating instructions,

please contact your NuAire Representative of NuAire technical Services.

1.3 Explanation of Symbols

!

!

NOTE:

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death of serious injury.

CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury.

CAUTION used without the safety alert symbol indicates a potentially hazardous situation which, if not avoided, may result in property damage.

Potential electrical hazard, only qualified person to access.

Used for important information.

Biohazard

Ground, Earth

WARNING

CAUTION:

CAUTION

Lead Free

Chemical Hazard

Flammable Hazard

Hazardous Gases! Personal Protection Equipment Required.

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2.0 Models & Features

The Model NU-602, Class II, Type A2 Laminar Flow Biological Safety Cabinet is manufactured in three sizes: 4 ft. (0.9m), 5 ft. (1.2m), and 6 ft. (1.8m).

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2.1 Optional Systems

The NU-602 Biological Safety Cabinet can be purchased with several operational systems which can greatly improve work flow, increase flexibility, and improve cleanliness in the handling of small animals. These systems are limited to factory installation and must be purchased when the basic cabinet is purchased. These systems are identified as the following: 1. Sterile Cage Delivery Option 2. Dirty Cage Collection Option 3. Refuse Disposal Option

2.1.1 Sterile Cage Delivery Option

The NuAire Model NU-602 is offered with a Sterile Cage Delivery option. Space requirements for this system limit installation to the 6 foot cabinet. Sterile cages are delivered under wraps to the cage changing station and placed in the delivery system where wraps are removed, twelve or more mice cages or 6 rat cages will maintain sterility because they are constantly "bathed" in HEPA filtered air (99.99% efficient for removal of 0.3 micron particles). The delivery system contains a spring balanced platform that moves vertically to place a sterile cage(s) at a position where they can easily be picked up by an operator. As each cage is removed from the stack of cages on the platform, the platform rises to place another sterile cage in position to be easily picked up. The HEPA filtered air flows down (around) the cages continuously drawn finally into the cabinet blower system where 70% of the air is recycled through the supply HEPA filter and 30% is exhausted to the room through an exhaust HEPA filter (see Drawing BCD-05440).

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2.1.2 Dirty Cage Collection Option The NuAire Model NU-602 is offered with a Dirty Cage Collection Option (see Drawing BCD-05441). This option permits an operator to change animals from a dirty cage to a clean cage and place the dirty cage (complete with old bedding, etc.) through a door in a wall of the cabinet into a bag for autoclaving. Thus, the operator has the personnel protection of a biological safety cabinet, the animal is protected from contamination and the soiled cages are dispensed in a convenient manner ready for sterilizing (animal cages are not furnished with this option). This option can be manufactured into either both left or right sides of the cabinet and is available in all three sizes.

Typically, a stack of sterile cages would be placed on the right side of the work tray. In the biological cabinet, a dirty cage complete with the mouse would be brought into the biological cabinet work zone on the left side (one-at-a-time). The mouse would be transferred from the dirty cage to a clean cage which would have been serviced as necessary. The dirty cage would then be placed into the collection bag to be autoclaved.

The operator can build a stack of 8 dirty cages placing them one-at-a-time into the autoclave bag. The cage support platform is adjustable up/down, but the nominal setting of the platform is 8-1/2 inches (216mm) below the bag attachment. Subsequent cage placing procedures may dictate a more comfortable position to the operator. The autoclave bag is stripped over the bag attachment; tight fit is desirable, but even a slightly large bag can be held in place with a bit of tape in a few places while the "O" ring performs the final retaining action.

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2.1.3 Refuse Disposal Option

The NuAire Model NU-602 is offered with a Refuse Disposal Option. Space requirements for this system limit the installation to the 6 foot (1.8m) cabinet. The disposal function is displayed on the left side work tray with the right side of the work tray used for processing the animal cages. Refuse is dumped into a double bag system, with the outer bag fastened to the underside of the plenum below the work tray and outer bag fastened to a metal ring member that fits snugly into the work tray. The ring member is lifted out of the work tray to permit an operator to fasten the inner bag. Both bags fit snugly to metal rings, but are also retained by "O" rings. The diameter of both bag rings is approximately 14-1/2" (368mm) or 45" (1143mm) in circumference. The outer bag seals the interior of the cabinet from the room and the inner bag carries the refuse. In preparing to dispose of the refuse, the inner bag is released from the metal ring in the work tray and dropped into the outer bag. Then the outer bag is released, tied and the refuse can is transported to an autoclave for sterilization of the bags. NuAire does not supply replacement bags. Replacement bags may be obtained, using the above ring specifications, in various laboratory supply catalogs. Typically, dirty cages with animals would be brought into the station on the left side. Clean cages would be stacked in the station on the right side having been autoclaved with bedding already installed. Animals would then be transported one at a time into the clean cages and the dirty cage would be dumped into the refuse bag. The dirty cage would then be subjected to normal procedure cleaning and sterilization (see Drawing BCD-05439).

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3.0 Warranty

NuAire, Inc. warrants that it will repair F.O.B. its factory or furnish without charge F.O.B. its factory a similar part to replace any material in its equipment within 36 months after the date of sale if proved to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories are warranted only to the extent guaranteed by the original manufacturer, and this warranty shall not apply to any portion of the equipment modified by the user. Claims under this warranty should be directed to NuAire, Inc. setting forth in detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment. This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse, abuse, accident, shipping damage, improper installation or service, or damage by fire, flood or acts of God. If the serial number of this product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety. The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any item, for any reason, contact NuAire for a Return Authorization Number. This number must accompany all returns. Any product shipped to NuAire without this number will be returned refused shipment or collect freight.

4.0 Shipments

NuAire takes every reasonable precaution to assure that your LABGARD cabinet arrives without damage. Motor carriers are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can occur in any shipment and the following outlines the steps you should take on receipt of a NuAire LABGARD cabinet to be sure that if damage has occurred, the proper claims and actions are taken immediately.

4.1 Damaged Shipments

4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.

4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the

consignee's copy of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis of your filing the claim against the carrier.

4.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT

AT ONCE and, request an inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date and that he failed to comply with the request. This along with other papers in the customer's possession will support the claim.

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5.0 Installation Instructions

5.1 Location Within the laboratory, etc., the ideal location of the biological safety cabinet is away from personnel traffic lanes, air vents (in or out), doors and/or any other source of disruptive air currents. If drafts or other disruptive air currents exceed the intake velocity of the cabinet through the access opening, the potential exists for contaminated air to exit or enter the work tray area of the cabinet. It depends on the severity of the air current. REMEMBER: A BIOLOGICAL SAFETY CABINET IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE. W Where space permits, a clear 6" (152mm) area should be permitted on each side of the cabinet for maintenance purposes. The electrical outlet into which the cabinet is connected should be readily accessible for maintenance purposes. Do not position the cabinet to prevent access to the power cord. The power cord plug serves as the disconnect and should remain readily accessible. If the outlet is inaccessible, such as a conduit (hardwired) connection, then an appropriate warning label should be applied near the cabinets on/off switch to indicate the circuit breaker on the power distribution panel to be used. A MINIMUM CLEARANCE OF 6" (152MM) IS REQUIRED FROM THE TOP OF THE CABINET TO THE CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX.

If it is desired to exhaust the cabinet to the outside, NuAire offers two general categories of exhaust transitions which will capture the exhaust efflux from the cabinet. These are:

1. Canopy, Thimble or Air Gap Exhaust Transitions (with and without integral fan) Strongly Recommended

2. Gas-Tight Exhaust Transitions

THE EXHAUST SYSTEM SHOULD BE FITTED WITH A BACKDRAFT DAMPER TO PREVENT REVERSING OF AIRFLOW IN THE SYSTEM. Both types of transitions have some common attributes, in addition to some that are unique. NuAire strongly recommends a canopy or thimble exhaust for most applications. See separate instruction sheets for a discussion of exhaust transitions and installation requirements.

5.2 Set-Up Instructions

Remove outer shipping protection (carton or crating). The cabinet is fastened to the base skid and it is usually the best procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate ease in handling. It can then be removed from the skid by removing the brackets, bolts and screws holding the cabinet to the skid.

The unit is shipped for on-site assembly of the following item:

Exhaust Filter Guard

NOTE:

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!

5.2.1 Exhaust Grill Installation The exhaust HEPA filter is protected with a cardboard cover for shipping. Remove the cover

and install the expanded metal exhaust guard to the exhaust chamber with 1/4-20 acorn nuts and washers provided. If an exhaust transition is being installed, the guard is not required.

5.2.2 Gas Service

NuAire doesn't recommend the use of natural gas within the LFBSC, but if gas service is determined to be necessary for the application, appropriate safety measures must take place. All NuAire LFBSC's have precautionary warning labels that say the following:

CAUTION Use of explosive or flammable substances in this cabinet should be evaluated by your

appropriate safety personnel.

Once the determination has been made by the appropriate safety personnel, the application of natural gas must be performed in accordance to national, state and local codes. IT IS ALSO STRONGLY RECOMMENDED THAT AN EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST OUTSIDE THE LFBSC ON THE GAS SUPPLY LINE. All NuAire LFBSC's meet the safety requirements of UL and CSA for Laboratory Equipment. To comply with these safety requirements, NuAire uses only certified gas valves. In addition, if external piping is required, only black pipe is used for this application. As previously stated NuAire doesn't recommend the use of natural gas within the LFBSC and ASSUMES NO RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The Bunsen burner flame within the LFBSC not only contributes to heat build-up; is also disrupts the laminar air stream, which must be maintained for maximum efficiency. IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS STRONGLY RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS. During use, the Bunsen burner should be placed to the rear of the workspace where resulting air turbulence will have a minimal effect.

5.2.3 Plumbing Services

Ground key cocks with the type of service specified by the removable button on the handle, are located in the work zone. The Ground Key cocks are not recommended for pressure over 30 psi (2.0 BAR). Reducing valves should be installed external to the cabinet if necessary. Ground key cocks should never be used for flammable gasses or oxygen service. A special needle for oxygen service or certified valve is required and available upon request.

External connection is to 3/8” (38mm) NPT coupling in the inner sidewalls. Plugged clearance holes in the outer sidewalls are provided for easy access to the inner couplings via a NPT nipple. Connection to plant utilities should be made with proper materials for the individual service and according to national and/or local codes. Observe all labels pertaining to the type of service and operating pressure.

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NOTE:

NOTE:

NOTE:

5.2.4 Electrical Services The NU-602 series Biological Safety Cabinets may be "hardwired" (optional) or connected via an electrical power cord which is standard. The unit requires 115 or 230 VAC, 60 or 50Hz, single phase (current rating varies per cabinet size, reference Electrical/Environmental Requirements). It is recommended that power to the unit be on its own branch circuit, protected with a circuit breaker or fuse at the distribution panel. THIS UNIT CONTAINS ELECTRONIC BALLASTS FOR THE FLUORESCENT LIGHTING. ELECTRONIC BALLASTS OPERATE WITH HIGH INRUSH CURRENT. IT IS NOT RECOMMENDED TO USE THIS PRODUCT WITH GROUND FAULT INTERRUPTERS (GFCI'S) BECAUSE THE BALLASTS MAY CAUSE THE GFCI TO TRIP.

5.2.5 Final Assembly

REMOVE THE PROTECTION CARDBOARD COVER OVER THE EXHAUST HEPA, located under the protective screen. The exterior surfaces and viewing glass are easily cleaned with any mild household detergent cleaner using a soft cloth. Harsh chemicals, solvent-type cleaners and abrasive cleaners should not be used. Do not attempt to clean the HEPA filter media. Cabinet interior walls or work surface are easily cleaned with any mild household detergent cleaner using a soft cloth. Turn the cabinet on and let it operate for 60 minutes before using it as a LFBSC.

5.3 Certification Testing Methods and Equipment

After installation and prior to use, NuAire recommends that the cabinet be certified or commissioned to factory standards. At a minimum, the following tests should be performed.

1. HEPA filter leak test 2. Downflow velocity test 3. Inflow velocity test 4. Airflow smoke patterns

The testing methods and equipment required are specified on the factory inspection report included with this manual (see insert in back cover).

IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR WITH THE METHODS AND PROCEDURES FOR CERTIFYING BIOLOGICAL SAFETY CABINETS (SEE INSERT).

AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT A MINIMUM ON AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE OR MAINTENANCE ACTION OR ANY TIME THE OPERATOR FEELS IT IS NECESSARY.

Note that the Labgard cabinets, filters and seals provide premium performance; Quality Control in both design and manufacturing assure superior reliability. However, protection to both product and operator is so vital that certification to the performance requirements should be accomplished as stated to ensure biological safety established by the factory standards.

NOTE:

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Labgard Class II, Type A2 Laminar Flow Biological Safety Cabinet

Models NU-602-4XX, 5XX, 6XX NU-602-4XXE/5XXE/6XXE

Catalog Number Catalog Number

NU-602-4XX Nominal 4 foot (0.9m)

NU-602-5XX Nominal 5 foot (1.5m)

NU-602-6XX Nominal 6 foot (1.8m)

Performance Specifications 1. Personal Protection 2. Product Protection

NSF/ANSI 49 NSF/ANSI 49

NSF/ANSI 49 NSF/ANSI 49

NSF/ANSI 49 NSF/ANSI 49

NSF Std. No. 49 Class Class II, Type A2 Class II, Type A2 Class II, Type A2 Style of Cabinet Console Console Console Cabinet Construction Pressure Tight Design

All welded stainless steel 16GA, Type 304 14GA CRS Base

All welded stainless steel 16GA, Type 304 14GA CRS Base

All welded stainless steel 16GA, Type 304 14GA CRS Base

Diffuser for Air Supply (Metal) Non-flammable Non-flammable Non-flammable HEPA Seal Type: Supply Filter-99.99% Eff. on 0.3 microns Exhaust Filter-99.99% Eff. on 0.3 microns

HEPEX Seal* Neoprene, Springloaded

HEPEX Seal* Neoprene, Springloaded

HEPEX Seal* Neoprene, Springloaded

Fumigation per NIH/NSF Procedure Yes Yes Yes Standard Services: Gas Cocks 3/8" NPT Duplex Outlet

One, Right Sidewall One, Right Sidewall

One, Right Sidewall One, Right Sidewall

One, Right Sidewall One, Right Sidewall

Optional Services: Gas Cocks 3/8" NPT Duplex Outlets Ultraviolet Light

Up to 3 ea. Sidewall 1 ea. Sidewall One, Backwall

Up to 3 ea. Sidewall 1 ea. Sidewall One, Backwall

Up to 3 ea. Sidewall 1 ea. Sidewall One, Backwall

Cabinet Size: inches (mm) Height Width Depth

77 (1956) 50 1/2 (1283) 31 3/4 (806)

77 (1956) 62 1/2 (1588) 31 3/4 (806)

77 (1956) 74 1/2 (1588) 31 3/4 (806)

Work Access Opening: Standard Opening Height - inches (mm) Standard Inflow Velocity (DIM)

12 (305) 105 FPM (.53m/s)

12 (305) 105 FPM (.53m/s)

12 (305) 105 FPM (.53m/s)

Work Zone: inches (mm) Height Width Depth

26 1/2 (673) 46 1/2 (1232) 24 1/2 (622)

26 1/2 (673) 58 1/2 (1486) 24 1/2 (622)

26 1/2 (673) 70 1/2 (1791) 24 1/2 (622)

Viewing Window: inches (mm) Hinged Tempered Glass

12 (305mm) opening

12 (305mm) opening

12 (305mm) opening

Minimum Exhaust Flow Standard 407 CFM (629CMH) 512 CFM (870 CMH) 617 CFM (1048CMH) Plant Duct Static Pressure 0.05-0.1" H20 0.05-0.1" H20 0.05-0.1" H20 Heat Rejected, BTU, Per Minute 24 35 43 Electrical: 115V (230V) Volts, AC 60/50 Hz Amps: Blower/Lights Amps: Duplex Amps: Total 12 ft. Power Cord (one)

UL/UL-C Listed (115V only) 115/60 (230/50,60) 10 (7) 3 (3) 13 (10) 12 GA - 3 Wire, 20A

UL/UL-C Listed (115V only) 115/60 (230/50,60) 12 (8) 3 (3) 15 (11) 12 GA - 3 Wire, 20A

UL/UL-C Listed (115V only) 115 (230/50,60) 12 (8) 3 (3) 15 (11) 12 GA - 3 Wire, 20A

*Crated Shipping Weight: Net Weight

815 lbs. 780 lbs.

915 lbs. 880 lbs.

1015 lbs. 980 lbs.

*Crated shipping weight does not include weight for accessories or options.

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6.0 Operating the NU-602 6.1 Operator Controls & Indicators

The following is a description of the controls and indicators, left-to-right on drawing BCD-06719. 6.1.1 Minihelic Gauge

The unit is normally equipped with a minihelic gauge. The minihelic gauge displays the static pressure within the pressure plenum supplying the downflow and exhaust filter. The gauge is calibrated in "inches of water gauge" or "millimeter of water gauge" pressure. As the HEPA filters load with particulate matter, the amount of static pressure will increase, giving an indication of the "health" of the cabinet. The initial pressure reading will be approximately 0.50" w.g. ± 0.05" w.g. (12mm w.g. ± 3mm w.g.).

6.1.2 Indicator Light

An indicator light is located above the blower on/off switch and lights when power is applied to the motor blower.

6.1.3 Blower Switch

The blower switch applies power to the internal blower/motor when in the "ON" position. The blower switch also has a second set of poles, which are available for use as a contact closure for an exhaust system. In addition, power is applied to the accessory outlet located in the rear of the service control center.

The purpose of the outlet is to provide "switched" power for the following:

1. Exhaust transition with integral blower, or 2. To power a low power appliance, such as an Airflow Monitor.

The rating of the outlet is 115 Vac at 2 amps maximum. The 230 Vac cabinets do not include this outlet as standard.

6.1.4 Fluorescent/U.V. Light Switch

This switch provides on/off control for the fluorescent light and/or the ultraviolet (U.V.) light if present (optional). With the U.V. light option, the switch provides for on-center off-on operation so that both the fluorescent light and the U.V. light cannot be energized at the same time. Proper care should be exercised when the U.V. light is on.

6.1.5 Circuit Breaker-Blower

The Blower is protected with a circuit breaker. The circuit breaker in conjunction with the motor's thermal protector is designed to open under locked rotor or half-wave power conditions. Should the circuit breaker open (pop-out button will appear) merely depress to reset. If the circuit breaker continually opens, a failure has occurred in the motor or solid-state speed controller. Consult a qualified repair technician or NuAire, Inc. for replacement.

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6.1.6 Outlet Switch

This switch provides on/off control for the 115 or 230 Vac power available in the outlet within the interior or the cabinet.

6.1.7 Circuit Breaker-Outlets

The outlet located in the sidewall of the work area is protected with a 3 amp circuit breaker. The circuit breaker may trip at 110% of load rating but will trip at 145% of load rating in less than 2 seconds. Should the circuit breaker open, (pop-out button will appear), unplug the appliance plugged into the outlet and merely depress the pop-out button to reset.

6.1.8 Airflow Control

The operating airflows within the cabinet (i.e. 75 LFPM (.38 m/s) downflow and 105 LFPM (.53 m/s) air inflow barrier) are controlled by a potentiometer and an exhaust damper. The potentiometer is located under the hinged cover of the service control center, in the left-rear corner. The shaft of the potentiometer has been painted red for easy identification.

The potentiometer controls the operating voltage applied to the motor/blower. The potentiometer is adjustable over 270 degrees with a slotted screwdriver, which varies the applied voltage. This adjustment should only be made by a qualified technician employing a thermoanemometer or velometer in order to insure airflows.

6.2 Auto-Lift Operating Controls

The following is a description of the controls and indicators (see Drawing BCD-05456). 6.2.1 Power Switch

The power switch applies power to the auto-lift control system. A green light within the switch indicates an "ON" state.

6.2.2 Mode Switch

The mode switch has three modes of activation, full down, run and lock. The full down mode is used to return the Auto-Lift down to its lowest position from any raised position. The run mode is used to alter the Auto-Lift up or down into a desired position. The lock mode is used to lock the Auto-lift system in any raised position.

6.2.3 Worksurface Height Switch

The worksurface height switch allows for the precise selection of the worksurface height in inches (cm). The worksurface height switch is used in conjunction with the mode switch in the run position. Changes to the worksurface height can be made at anytime. CAUTION: Lift system is designed for a 10% duty cycle with a maximum on time of 2 minutes.

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NOTE:

6.3 Operating Guidelines The intent herein is to present general operational guidelines that will aid in the use of the Laminar Flow Biological Safety Cabinet (LFBSC) to control airborne contaminants of low to moderate risk as stated in Technical Report No. FPS 56500000001, prepared by Dow Chemical U.S.A., for the National Cancer Institute, May 1, 1972.

Procedure protocols defined in terms of the barrier or control concepts unique to LFBSC must be developed in order to obtain a maximum potential for safety and protection. The pre-planning necessary to develop these protocols is based on several fundamental considerations, each of which will contribute to optimum benefits from the equipment:

a. Know your "Safe Working Area" b. Minimize disruption of "air curtain" c. Minimize room activity d. Utilize unidirectional airflow e. Employ aseptic techniques

6.3.1 Know Your "Safe Working Area" The LFBSC safe working area is basically the worktray or depressed area. All work should be performed on or above the worktray. The area on or above the front grill is a non-safe working area. 6.3.2 Minimize Penetration of "Air Curtain"

The minimum number of items necessary should be placed into the cabinet to prevent overloading, but the work should also be planned to minimize the number of times an operator's hands and arms must enter and leave the air curtain at the open face. The ideal situation is to have everything needed for the complete procedure placed in the hood before starting, so that nothing need pass in or out through the air barrier at the face until the procedure is completed. This is especially important in working with moderate risk agents.

Unnecessary rising of the hands inside the cabinet above the level of the work opening should be avoided. This presents an inclined plane from hands to elbows along which the downflow of air may run to, and possibly out, the open face.

When working with agents of lower risk, it is not as important for all materials to be placed in the cabinet before starting, or for the procedure to be completely finished before materials are removed. Also, the time period for a unit may be continued over a more extended period during which entries and withdrawals from the cabinet may be made.

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! CAUTION

6.3.3 Minimize Room Activity Activity in the room itself should be held to a minimum. Unnecessary activity may create disruptive air currents as well as interfere with the work of the operator. A person walking past the front of a cabinet can cause draft velocities up to 175 fpm, which are sufficient to disrupt the air balance of the laminar flow unit.

6.3.4 Utilize Unidirectional Air Flow The operator must keep two important facts in mind: (1) The air, as supplied to the work area through filters from the top, is contaminant free and (2) Airborne contamination generated in the work area is controlled by the unidirectional flow of parallel air streams in a top-to-bottom direction.

A solid object placed in a laminar air stream will disrupt the parallel flow and consequently, the capability of controlling lateral movement of airborne particulates. A cone of turbulence extends below the object and laminarity of the air stream is not regained until a point is reached downstream, approximately equal to three to six times the diameter of the object. Within the parameters of this cone, particles may be carried laterally by multidirectional eddy currents.

Transfer of viable materials and manipulations which may generate aerosols should not be performed above sterile or uninoculated materials. Items should be localized on the work surface in "clean" and "dirty" groups.

6.3.5 Employ Aseptic Technique

The operator must not assume an attitude of "let the cabinet do it" when performing procedures within a LFBSC. Properly balanced and properly used cabinets will do an excellent job of controlling airborne contamination and containing viable agents, but the cabinet will not eliminate contact transmission of contamination. Normal laboratory contamination control procedures and basic aseptic techniques are necessary to obtain maximum benefit from the cabinet. For example, open bottle, tube or flask mounts should be kept as parallel as possible to the downflow to minimize capture of chance particulates. This precaution is merely an extension of good aseptic technique as practiced on open bench tops. The good laboratory practices designed to minimize creation and/or release of aerosols to the environment should not be discontinued.

Items of equipment in direct contact with the etiologic agent must remain in the cabinet until enclosed or until surface-decontaminated. Trays of discard pipettes must be covered before removal from the cabinet (aluminum foil may substitute for fabricated covers). If an accident occurs which spills or splatters suspensions of etiologic agent around the work area, all surfaces and items in the cabinet must be surface-decontaminated before being removed.

Applying a burner flame to flask and tube necks when mating surfaces of sterile assemblies is a conventional method of minimizing chance contamination. However, the efficiency of this operation is usually related to the removal of airborne contamination occurring while the item is uncovered. If the manipulation is carried out in an environment free of airborne particulates, then the need for the flaming operation is essentially removed. This is one of the additional advantages of the LFBSC - use of the gas burner is seldom necessary. The gas burner flame in one of these units not only contributes significantly to the heat build-up, it also disrupts the laminar air streams which must be maintained for maximum efficiency. IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS. It should also be placed to the rear of the work space where resulting air turbulence will have a minimal effect. If cabinet air is inadvertently turned off, the flame could damage the HEPA filters.

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6.4 Ergonomics Ergonomics, the study or accommodation of work practices is extremely important for proper cabinet usage and user health and safety. An evaluation of normal work practices should be performed with each user when working in a cabinet. Evaluation criteria should be at a minimum: a. Proper user posture b. Effective workzone layout for work practice c. Vision or sightlines For each of the above evaluation criterion, several aids may be supplied to accommodate the user. • Ergonomic chair - A six-way articulating seat and back control for personalized adjustment to assure

proper user posture. Be sure feet are resting on the floor, chair foot support or foot rest. Also be sure back is fully supported with proper chair adjustments.

• Forearm/elbow support - The cabinet is provided with a forearm support on the work access opening. Periodic mini-breaks during work practice should be taken resting forearm to avoid stress and fatigue. Elbow rests are optional that can provide support for particular work practices, such as pipetting.

• Effective workzone layout - Always prepare your work procedure to minimize reach to avoid neck and shoulder stress and fatigue. Rotating tables are optional to maximum workzone and minimize reach.

Vision and sightline - Always prepare your work procedure to eliminate glare and bright reflections on the window. Keep your window clean and sightlines clear to your effect workzone.

6.5 Operating Sequence 6.5.1 Start Up

Turn on cabinet blower and lights, check air intake and exhaust portals of the cabinet to make sure they are unobstructed. Many of the cabinets are provided with magnehelic gauges which indicate pressure differentials across the supply filters. They may indicate to the LFBSC maintenance technician when to replace the filters, dependent upon the blower fan capacity. Blower speed must only be readjusted by qualified maintenance technicians.

Note: Some cabinets are equipped with ultraviolet (UV) lights. These must be turned off

during the day while laboratory personnel are occupying the room. Good procedure includes the decontamination or wipedown of cabinet surfaces with chemical disinfectant before work commences. This practice eliminates the need for UV lights, whose primary utility in this application is inactivation of surface contamination since the filters effectively remove all airborne contaminants. UV lights, therefore, are not recommended in the LFBSC.

Allow blowers to operate for a minimum of 15 minutes before aseptic manipulations are begun in the cabinet. If the filtered air exhausted from the unit is discharged into the room, as in some installations, an additional advantage is obtained from purification (filtration) of the room air circulated through the equipment. Because of this characteristic contributing to the quality of the laboratory environment, some owners of LFBSC leave them in operation beyond the time of actual use.

6.5.2 Wipedown The interior surfaces of the work space should next be disinfected (see cleaning procedures) by wiping them thoroughly with 70% alcohol or similar non-corrosive antimicrobial agents. DO NOT USE ANY CHLORINATED OR HALOGEN MATERIALS IN THE CABINET.

6.5.3 Materials & Equipment

The apparatus and materials should next be placed into the cabinet. Care must be exercised that no items be placed over the front intake grills. Materials should be arranged so that clean, dirty (used), and virus materials are well separated. Passage of contaminated materials over uninoculated cultures or clean glassware should be avoided and transfer of viable materials should be performed as deeply into the cabinet (away from open face) as possible.

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6.5.4 Air Purge

Additional purging of the work space without user activity should be allowed for 2-3 minutes after materials and apparatus have been placed in it. This will rid the area of all "loose" contamination that may have been introduced with the items.

6.5.5 Perform Work

The work can now be performed. The technician performing the work is encouraged to wear a long-sleeved gown with knit cuffs and rubber gloves. This will minimize the shedding of skin flora into the work area and concurrently protect the hands and arms from viable agent contamination. At a minimum, the hands and arms should be washed well with germicidal soap before and after work in the cabinet. For the preparation of antineoplastic drugs, the following procedures summarize those contained in OSHA Instruction PUB 8-1.1, "Work Practice Guidelines for Personnel Dealing with Cytotoxic (Antineoplastic) Drugs." The above document should be thoroughly studied / reviewed prior to drug preparation in the cabinet.

a. A sterile plastic-backed absorbent drape should be placed on the work surface during

mixing procedures. The drape should be exchanged whenever significant spillage occurs, or at the end of each production sequence.

b. Vials should be vented with a filter needle to eliminate internal pressure or vacuum. c. Before opening ampules, care should be taken to insure that no liquid remains in the tip

of the ampules. A sterile gauze sponge should be wrapped around the neck of the ampules while opening.

d. Final drug measurement should be performed prior to removing the needle from the stopper of the vial.

e. A non-splash collection vessel should be available in the biological safety cabinet to discard excess drug solutions.

6.5.6 Terminal Purging & Wipedown

Following completion of work, allow the cabinet to run for 2-3 minute period without personnel activity to purge the unit. The decontamination of the interior surfaces should be repeated after removal of all materials, cultures, apparatus, etc. A careful check of grills and diffuser grids should be made for spilled or splashed nutrients which may support fungus growth and resulting spore liberation that contaminates the protected work environment.

6.5.7 Prefilter

Located under the work surface is a prefilter area. This area forms the return air path to the motor/blower. If the air flow is blocked by paper or the prefilter is "loaded", the performance of the cabinet could be seriously affected. Therefore, THE PREFILTER SHOULD BE CHECKED AND CLEANED NO LESS THAN ON A WEEKLY BASIS; on a daily basis if procedures dictate the use of paper products. Any paper removed must be properly disposed of as Contaminated Hazardous Waste.

6.5.8 Shut Down

Turn off blowers and lights. Do not use cabinet as a depository for excess lab equipment during periods of non-operation. If the antineoplastic agents are being prepared in the cabinet, it is recommended to let the cabinet run 24 hours per day. This lessens the possibility that contaminants may escape.

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CAUTION:

6.6 Cleaning Procedures

Cleaning the cabinet is an important function in terms of both containment and sterility. Use the following procedure to effectively surface disinfect or clean the cabinet workzone surfaces. a. Raise the window to a full-open position, if desired. b. Apply appropriate disinfecting solution to cabinet surfaces. Most surface disinfectants require a

specific contact time depending upon the microbiological agents used within the cabinet. CONSULT APPROPRIATE DISINFECTANT DOCUMENTATION FOR PROPER APPLICATION AND SAFETY PRECAUTIONS.

DISINFECTANTS THAT USE CHLORIDES AND HALOGENS WILL CAUSE DAMAGE TO THE STAINLESS STEEL SURFACES IF LEFT ON FOR LONG PERIODS OF TIME.

c. After the specified contact time, wipe up excess disinfectant. IF THE DISINFECTANT

USED CONTAINS CHLORIDES OR HALOGENS, RE-WIPE ALL SURFACES WITH 70% ALCOHOL OR SIMILAR NON-CORROSIVE ANTI-MICROBIAL AGENT TO PREVENT DAMAGE TO STAINLESS STEEL SURFACES.

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191

ATTENTION ACCOMPANY'S INFORMATION OR IMPORTANT SYMBOL

POTENTIAL ELECTRICAL HAZARD ONLY QUALIFIED PERSON TO ACCESS

188

!

7.0 General Maintenance

CAUTION: All maintenance actions on this equipment must be performed by a qualified technician who is familiar with the proper maintenance procedures required for this equipment. This includes both certification as well as repair.

7.1 DecontaminationNo maintenance should be performed on the interior of the Labgard cabinet (area behind access panels) unless the cabinet has been microbiologically decontaminated, is known to be biologically clean, or known to be chemically inert. Surface disinfection is performed as specified in the cleaning procedures.

CAUTION! Hazardous Gases! Personal Protection Equipment Required.

A disinfection using formaldehyde must be performed in accordance with the specifications of NSF 49/1992, Annex G.

This procedure presents considerable risks and must be performed only by specially trained and authorized service personnel in accordance with applicable safety regulations.

The formaldehyde is vaporized within the tightly sealed sample chamber. The quantity of the applied formaldehyde depends on the volume of the sample chamber in the safety cabinet that is to be disinfected. The formaldehyde evaporates immediately after reaching its boiling point; the minimum reaction time is 6 hours. Therefore, the formaldehyde should be neutralized after the

specified reaction time by vaporizing ammonia.

CAUTION! Flammable Hazard!

Formalin is flammable. The auto-ignition temperature of formalin is 430° C (820° F).

With a volume percentage of 7.75% in dry air, formaldehyde vapor may explode.

For vaporization, do not use heating devices reaching temperatures above 250° C (477° F).

CAUTION! Chemical Hazard!

Formalin in reaction with hydrogen chloride will form BCME which is a hazardous chemical.

When using formalin, all residues of hydrogen chloride in the work chamber of the cabinet must be removed.

!

!

!

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If microbiological decontamination is necessary, use the following procedure: 1. Remove hinged window if desired. 2. Place decontamination equipment inside the work area. Reference decontamination procedure,

per NSF Standard 49, Annex G, using the following chart to calculate chemical requirements.

Cabinet Size -4XX/E -5XX/E -6XX/E Approximate Cabinet Dimensions

70-1/2 H x 30D x 46-1/2W (1.79 x .762 x 1.18m)

70½H x 30D x 58-1/2W (1.79 x .762 x 1.49m)

70½ x 30D x 70-1/2W (1.79 x .762 x 1.79m)

Cabinet Volume

56.9 cu. ft. (1.61 cu. m)

71.6 cu. ft. (2.03 cu. m)

86.3 cu. ft. (2.44 cu. m)

Note the outlets in the work area are energized as long as the cabinet is plugged in and switched on the front panel. Unplug the cabinet before decontamination equipment is plugged into these outlets or run the decontamination power cords under the front seal area. 3. Use duct tape and plastic to seal the front and exhaust area. CAUTION: BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY LABORATORY EXPOSURE TO DECONTAMINATION GAS. 4. Perform decontamination procedure per NSF Standard 49, Annex G. If the cabinet has been used to prepare antineoplastic drugs, (chemotherapy), or other toxic chemicals, decontamination of the cabinet cannot be accomplished by the above procedure. It is recommended that the following protective measures be taken:

1. Gloves

Gloves must be worn. Care must be taken not to cut, puncture, or tear the gloves. No one glove material is impervious to all CYTAs; disposable surgical or polyvinyl chloride (PVC) gloves provide substantial but not complete protection. PVC gloves probably are more protective than surgical gloves, but they are stiffer and less tactile. Gloves should be discarded after each use. Gloves should be tucked into the cuffs of the gown. Double gloving should be considered.

2. Face & Eye Protection A disposable dust and mist respirator and either a plastic face shield (preferred) or chemical splash goggles must be worn. The face shield or goggles should be wiped clean with a suitable tissue and water after each use.

3. Gowns A protective garment must be worn. The garment should be made of lint-free, low-permeability fabric and must have a closed front, leg sleeves, and elastic or knit closed cuffs. Tyvek1 isolation gowns are one example of an acceptable garment. The garment must be worn outside the work area. Disposable gowns are preferred over reusable. Front-buttoned coats are not recommended.

4. Hair & Shoe Covers Disposable hair and shoe covers should be worn.

5. Motion Slow and deliberate motions are necessary when working in the interior of the cabinet, in order to minimize the generation of particulates.

Please consult with NuAire, Inc. about any unique contamination problems.

Normally, no preventive maintenance is required on the interior of the cabinet (i.e., the area behind the access panel containing the HEPA filters and motor (blower assembly). All required adjustments in order to maintain proper cabinet airflows are external to the cabinet interior. The motor is lubricated for life and is thermally protected with automatic reset.

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!

7.2 Fluorescent Lamp Bulb Replacement The bulb is fluorescent, cool white, high output (HO), rapid start and placed internal to the cabinet. The life rating of the bulb is 9000 hours based on three-hour burning cycles.

To replace a bulb, it is necessary to remove the bulb shield.

1. First, switch Cabinet Light Switch off. 2. The bulb is removed by displacing the bulb to one side against the compressible bulb holder. 3. Reverse the procedure to install the new bulb.

7.3 HEPA Filter/Motor Replacement (Drawing BCD-00919)

The HEPA Filters under normal usage and barring an accident (a puncture), do not need replacement until the efflux velocity cannot be maintained or the access inflow velocity cannot be maintained at 100 LFPM (.51 m/s) (min.). This may permit the average downflow velocity to be as low as 70 LFPM (.35 m/s) as long as no point falls below 20 percent of the average downflow velocity. The HEPA Filters should not be replaced until the entire cabinet has been decontaminated or known to be biologically "clean".

7.3.1 Procedure (see Drawing BCD-00919) CAUTION: Disconnect electrical power from the unit before attempting any maintenance action.

Step 1: Remove the exhaust filter access cover and the supply filter access cover, by removing

all acorn nuts, washers and lockwashers (many places).

The interior of the cabinet is now fully exposed for replacement of the filters.

Step 2: Filter Removal It is not always necessary to replace both the supply and exhaust filter at the same time although it may be cost effective to do so. If during the course of certifications, the downflow always falls off while the exhaust increases, the supply filter is "loading" faster than the exhaust filter and only the supply filter may need replacement. The opposite might also happen depending upon many factors.

a. To remove the supply HEPA:

1. First, release the pressure clamps (3 for 4 foot (.9m) and 5 for 5 and 6 foot (1.2m and 1.8m)). The clamps provide very high tension and may require mechanical assistance to unfasten.

2. Second, remove the filter. The plenum frame is hinged in the rear and must be raised slightly while removing the HEPA filter. Handles are provided in the front of the filter.

b. To remove the exhaust HEPA:

Release the tension on the exhaust filter clamps by turning the knobs clockwise (4 places). This will lower the pressure plenum exhaust frame to a level that permits removal of the HEPA.

CAUTION: Dispose of spent HEPA filters properly. Avoid direct contact to "dirty side" of the

filters. Label toxic waste. 1 Available from Lab Safety Supply, Janesville, WI 53547-1368, or other laboratory, industrial, or hospital supply distributors.

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Step 3: Filter Installation WHEN INSTALLING NEW FILTERS, USE ONLY FILTERS OF THE SAME RATED, FLOW AND SIZE AS ORIGINALLY INSTALLED. Both filters (supply and exhaust) require top and bottom gaskets which should be 1/4 inch (6mm) thick, closed all neoprene. A light coating of silicone or Vaseline grease should be applied to all gaskets. Care must be taken to assure that the filters are not punctured or the gaskets damaged when reinstalling the filters. Remove the handles from the front of the dirty filters and install on the new filters.

a. Supply filter installation:

Insert the supply filter through the front opening, tilting the filter upward to allow rear edge of filter to slide on the bottom rail, until the front edge of the filter rests on the front rail. Install the front clamps with flanged tip insert into the slot in front rail.

b. Exhaust filter installation:

Before installing the exhaust filter be sure to open the choke plate fully. This will assist in adjusting the airflow. Next, slide the exhaust filter all the way to the rear of the pressure frame. Turn the knobs evenly, counterclockwise until an even seal is obtained. Compress the gaskets about 1/8 inch (3mm), top and bottom.

Step 4: Motor/Blower Assembly Removal

a. The motor/blower is accessible by removing the front access plate in the cabinet base.

1. Remove front access plate via #8-32 screws on cabinet base. 2. Remove the HEPEX bag from the blower via the band clamp. 3. Disconnecting the electrical connections from the junction box on the blower housing or bulkhead connector on sidewall of base cabinet. 4. Remove the blower from the cabinet base via the four #1/4-20 nuts. 5. Re-install the new motor/blower assembly.

7.4 Auto-Lift System (Drawing BCD-05457)

The Auto-Lift has been factory set to adjust the worksurface height between 31 inches (787mm) and 38 inches (965mm). However, due to variances of the floor surface, both at NuAire's factory and at the installation site, some adjustments may be required. Please observe some items before any work is attempted on the Auto-Lift System.

1. Always begin making adjustments from the 31 inch (787mm) worksurface height. 2. Always use caution and observe safety measures when performing maintenance on the cabinet. 3. Never allow the unit to rise higher than 39 inches (991mm) measured at the work surface height. 4. Never leave hands, feet, or toes under the unit, especially when lowering cabinet.

7.4.1 Unbinding Telescoping Members

a. Due to floor difference between factory and installation site, the guides for the telescoping members may require re-alignment.

b. Please note that although these parts aren't holding the cabinet up, they do prevent

wobbling, so use caution not to move the cabinet when adjusting.

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7.4.2 Actuator Replacement a. Remove side and bottom cover panels via fasteners on cabinet front, rear &

bottom. b. If possible, lower cabinet to its lowest position and block cabinet to take load off the castors. c. Remove power from the cabinet.

CAUTION: THE BOTTOM PLATE WITH THE CASTOR ATTACHED WEIGHS NEARLY 50 POUNDS. USE EXTRA CARE NOT TO DROP IT ON HANDS OR FEET.

d. Remove cotter pins and clevis pins at top and bottom, disconnect wires at interconnect and remove motor.

e. Clamp base plate to cabinet base.

f. Install new actuator into position by attaching top pin first, then, rotate bottom tube into position for bottom pin attachment.

g. Re-attach bottom and side cover panels.

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NOTE:

NOTE:

7.5 Airflow Calibration

The NU-602 Airflow Calibration Consists of adjustments to balance the airflow within the cabinet. THIS WORK SHOULD BE DONE ONLY BY A QUALIFIED TECHNICIAN WHO CAN MEASURE THE AIRFLOW FROM THE FILTERS WITH A SUITABLE VELOMETER. NuAire provides two adjustments to balance the airflow within the cabinet. These are:

a. blower speed adjustment via motor voltage regulator b. exhaust filter choke

The blower speed controller adjusts the cabinet’s total volume of airflow while the choke adjusts or balances the exhaust airflow as well as makes up for filter resistance tolerances. Since it has been NuAire's experience that the filters may not "load" evenly, both adjustments are necessary for proper cabinet performance.

The cabinet is considered to be certifiable if the following airflow measurements are present:

a. Downflow Average: 75 LFPM ± 5 LFPM (.38 m/s ± .025 m/s). b. Inflow Average: 105 LFPM ± LFPM (.53 m/s ± .025 m/s) using the calculated inflow velocity measurement method. BEFORE STARTING AIRFLOW CALIBRATION PROCEDURE LET THE CABINET RUN FOR AT LEAST 10 MINUTES.

7.5.1 Downflow Calibration Step 1: • Place a velometer in the cabinet workzone on the horizontal plane defined by the

bottom edge of the viewing window. Spot check several points on the recommended downflow velocity test grid found in table 7.0.

Step 2: •

If necessary, adjust airflow control potentiometer, located in the control center to the above stated airflow requirements.

Step 3: •

Proceed to inflow calibration

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7.5.2 Inflow Calibration Step 1: •

Measure the inflow velocity using the recommended procedure found in Table 7.0. If necessary, adjust the exhaust filter choke, located under the front decorative panel, to achieve the correct average inflow velocity within the stated range of 105 ± 5 LFPM (.53 ± .025 m/s).

•

Less than 100 LFPM (.51 m/s); First, open the choke plate or make sure it is open. If this is insufficient, then increase the motor speed control.

•

Greater than 110 LFPM (.56 m/s); First, adjust the motor speed control to achieve 1/2 the exhaust excess, then close the choke plate to achieve the balance. In this fashion, the downflow should remain nearly constant (i.e. what the reduced speed took away, the choke plate restores).

Note: The choke plate adjustment requires a 3/16 inch (5mm) Allen wrench with a 6

inch (152mm) length. While monitoring the exhaust flow to check position, turning the set screw clockwise will open the choke while turning the set screw counter clockwise closes the choke.

Step 2: • Once exhaust adjustment is complete, return the downflow calibration and then

check average downflow velocity. If the downflow average remains within the correct range, the calibration is complete. If not, readjust as necessary to obtain the correct calibration range.

7.6 Filter Integrity Check

In order to check filter and filter seal integrity, the HEPA filter media and seals must be directly accessible, by the measuring instrument. The diffuser plate placed below the HEPA to protect the filter during normal usage may be removed as follows: The diffuser is secured to the cabinet shell by fasteners located immediately behind the front viewing window. After removing the fasteners, drop the front of the diffuser plate several inches and pull forward gently. Note that the diffuser is purposely a tight fit - it is secured to the back wall of the cabinet interior by a light push - fit with projecting studs.

The exhaust filter is typically more difficult to check, since charcoal filters, or exhaust transitions could cover the filter. Access panels are usually provided and should be removed. If an air gap exhaust transition is provided, the air gap must be sealed with duct tape or other suitable means to prevent contaminated air from migrating into the exhaust efflux. All exhaust blowers/fans should be turned off during the check.

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Table 7.0 Recommended Measurement Methods for Cabinet Downflow & Inflow.

A. Downflow Measurement

a. Instruments: TSI 8355 Thermoanemometer b. Procedure: Supply filter efflux is measured on a grid, in a horizontal plane defined by the

bottom edge of the window. No readings should be taken closer than 6 inches (152mm) from the inside perimeter.

c. Test Data - Inches (mm): 4XX/E 6 11.750 17.500 23.250 29.000 34.750 40.500

(152) (298) (445) (591) (737) (883) (1029) 5XX/E 6 11.812 17.625 23.437 29.250 35.062 40.875 46.687 52.500

(152) (300) (448) (595) (743) (891) (1038) (1186) (1334) 6XX/E 6 11.850 17.700 23.550 29.400 35.250 41.100 46.950 52.800 58.650 64.500

(152) (301) (450) (598) (747) (895) (1044) (1193) (1341) (1490) (1638) 6

(152) 11.750 (298) 17.5 (445)

d. Acceptance Criteria: 1. Average downflow velocity = 70 to 80 fpm (.35 to .41 m/s)

2. Individual readings must be within +20 percent of the average downflow velocity. B. Exhaust Air CFM/Access Opening Inflow Velocity (Applicable on all units)

a. Instrument: Shortridge Flowhood ADM-870 b. Procedure:

The inflow velocity is measured by using a Direct Inflow Measurement (DIM) Instrument (i.e. shortridge flowhood). The DIM Instrument should be equipped with a flowhood that is as close as possible to the width of the cabinet (i.e. NU-602-400 should use 1 x 4 foot flowhood). The DIM Instrument should also be duct taped to the cabinet to prevent any sneak air paths from occurring.

The DIM Instrument will read inflow volume (i.e. CFM). Use the window access opening area and correction factor to calculate inflow velocity.

Model NuAire Spec

(fpm) Calculated Access

Opening Area DIM

CFM=FPM Correction

Factor Results NU-602-

400/E 100-110 3.88 12" 371 = 96 96 + 9 (+9%) = 105 fpm NU-602-

500/E 100-110 4.88 12” 490 = 100 100 + 5 (+5%) = 105 fpm

NU-602-600/E 100-110 5.88 12" 555 = 94 94 + 11 (+12%) = 105 fpm

DIM

(CFM) CFM

= FPM Correction

Factor Actual FPM AREA

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C. Alternate Procedure:

a. Instrument: TSI 8355 Thermoanemometer. b. Procedure: Exhaust filter efflux is measured on a 4 x 4 inch (102mm) grid, 4 inches (102mm)

above the exhaust filter. The average access opening inflow velocity can be calculated from these measurements, since the exhaust volume is equal to the inflow volume.

c. Test Data - Inches (mm) Back Wall

Number of Readings

3.25 (83)

Ave. Exhaust. ft./min(m/s) Vel.

7.25 (184)

Exhaust Area ft.2(m2)

11.25 (2.86)

Calc. Exhaust ft.3/min.(m3/s) Volume

15.25 (387)

Access Opening ft.2(m2)

19.25 (489)

Inflow Velocity

400/E 4.250 (108)

8.250 (210)

12.250 (311)

16.250 (413)

20.250 (514)

500/E 4.250 (108)

8.250 (210)

12.250 (311)

16.250 (413)

20.250 (514)

24.250 (616)

600/E

4.250 (108)

8.250 (210)

12.250 (311)

16.250 (413)

20.250 (514)

24.250 (616)

28.250 (718)

32.250 (819)

d. Acceptance Criteria:

1. Access opening inflow velocity = 100 to 110 fpm (.51 to .56 m/s) e. Meets Acceptance Criteria: Yes ______ No ______

7.7 Cabinet Leak Tightness Test

The cabinet leak tightness test can be performed by several different methods. However, to perform the test, the window access area and the exhaust HEPA filter area must be sealed to pressurize the cabinet. The window access area may be sealed by a custom plate or plastic and duct tape. The exhaust HEPA filter area may also be sealed by a custom plate or plastic and duct tape. Sealing should be accomplished on the cabinet face and cabinet top to assure a flat sealable surface.

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8.0 Error Indicators & Troubleshooting Audible alarms and error indicators occur for a variety of reasons. Whenever an alarm condition is present, the audible alarm and error indicator will be presented and stay on until the error is cleared. When presented with an error indicator, please perform the following:

Step 1: NOTE ALL ERROR INDICATORS. When the cabinet is running, any and all red indicators display an error.

Step 2: VERIFY ERROR INDICATORS. Error indicators can be verified by turning the errored

function on/off. Step 3: MONITOR RE-OCCURRENCE OF ERROR INDICATORS. If re-occurrence of the error

indicator is immediate or daily, use guide below to correct the situation.

Error Indicator Troubleshooting Guide Error Indicator Indicator Correction Cabinet fluorescent lights won't turn on.

Check blower/light circuit breaker on front of control center. Check fluorescent lamps. Check voltage to light ballast's. Check ballast. Check light switch.

Cabinet blower won't turn on.

Check sliding window for correct operational height. Check blower/light circuit breaker on front of control center. Check voltage to blower. At motor voltage regulator and at bulkhead connector. Check wiring to blower. Check blower capacitor. Check sliding window blower cutoff microswitch. Check blower motor.

Cabinet outlets won't turn on.

Check outlet circuit breaker on front of control center. Check voltage to outlets.

Cabinet ultraviolet light won't turn on.

Check blower/light circuit breaker on front of control center. Check ultraviolet lamp. Check voltage to ultraviolet ballast's. Check ballast. Check light switch.

Blower/lights circuit breaker continues to trip after reset.

Check for short on output of circuit breaker. Replace circuit breaker. Isolate output of circuit breaker by disconnecting control center connectors, light circuit, motor voltage regulator, etc. to isolate the short.

Magnehelic gauge Magnehelic gauge

reads "NO" or "LOW FLOW"

Check Magnehelic gauge operation. Check for pinched tubing in control center. Make sure airflow is not too low.

9.0 Remote Contacts 9.1 Exhaust System Contacts

The exhaust system contacts are single pole normally open contact closure outputs which are activated whenever the blower switch is turned on. The contact points are located on the blower switch. Contact ratings are 250 VAC maximum at 2 Amps.

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10.0 Optional Equipment

10.1 Ultraviolet Light

CAUTION: Ultraviolet light will injure your eyes. Avoid direct viewing at all times. Personnel should not be present when ultraviolet lamp is on

10.1.1 Overview

The germicidal ultraviolet is primarily intended for the destruction of bacteria and other microorganisms in the air or on directly exposed surfaces. Approximately 95% of the ultraviolet radiations from germicidal tubes are in the 253.7 nanometer region. This is a region in the ultraviolet spectrum which is near the peak of germicidal effectiveness. The exposure necessary to kill bacteria is the product of time and intensity. High intensities for a short period of time, or low intensities for a longer period are fundamentally equal in lethal dosage on bacteria (disregarding the life cycle of bacteria). The intensity of light falling on a given area is governed by the inverse law; that is the killing intensity decreases as the distance increases from the tube. The germicidal tube is placed in the cabinet to provide an average intensity of 100 microwatts per square centimeter (for a new tube) falling on a horizontal plane defined by the center of the work surface. The minimum requirement per paragraph 5.12 of NSF Standard 49 is 40 microwatts per square centimeter (ref. NSF Std. #49, June, 1976).

10.1.2 Operation The operation of the ultraviolet light is accomplished by pressing the UV switch location on the front panel.

10.1.3 Precaution

The rays from germicidal tubes may cause a painful but temporary irritation of the eyes and reddening of the skin, if of sufficiently high intensity, or if exposure covers a prolonged period of time. For this reason, one should avoid direct eye and skin exposure to ultraviolet light. If exposure cannot be avoided, it is necessary for personnel to wear eye goggles or face shields, and long sleeve gowns with rubber gloves.

Since the window of the NU-602 does not close completely, it is recommended that personnel

leave the cabinet face area when in use. 10.1.4 Maintenance

The output of an ultraviolet light deteriorates with burning age. The useful life of the light is approximately 7000 hours under specific test conditions. NOTE: Before testing with lamp off, the light may be cleaned with a lint-free cloth dampened with alcohol or ammonia and water. It is recommended that either a time schedule be established or the tube's output be measured periodically and the tube replaced when its output falls below 40 microwatts per square centimeter or exceeds 7000 hours of operation. Lights should be allowed to operate approximately 5 to 10 minutes (longer when the light is in low temperatures) to warm up sufficiently before reading the output with a meter.

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Energies Required to Destroy Some Microorganisms by Ultraviolet Radiations (e)

Mold Spores

Microwatt seconds per cm/2

Protozoa

Microwatt seconds per cm/2

Penicillum roqueforti 26,400 Paramecium 200,000(a) Penicillium expansum 22,000 Penicillium digitiatum 88,000 Nematode Eggs 40,000(b) Aspergillus glaucus 88,000 Aspergillus flavus 99,000 Algae 22,000(c) Aspergillus niger 330,000 Rhizopus nigricans 220,000 Virus Mucor racemosus A 35,200 Baceriophage (E. Coli) 6,600 Mucor racemosus B 35,200 Tobacco Masaic 440,000 Oospora lactis 11,000 Influenze 3,400(d) Yeasts Saccharomyces 13,200 ellipsoideus 17,600 Saccharomyces cerevisiae 13,200 Brewers' yeast 6,600 Baker's yeast 8,800 Common yeast cake 13,200 Bacteria Streptococcus lactis 8,800 Strep. hermolyticus (alpha type) 5,500 Staphylococcus aureus 6,600 Staphylococcus albus 5,720 Micrococcus sphaeroides 15,400 Sarcina lutea 26,400 Pseudomonas fluorescens 7,040 Escherichia coli 7,040 Proteus vulgaris 7,480 Serratia marcescens 6,160 Bacillus subtilis 11,000 Bacillus subtilis spores 22,000 Spirillum rubrum 6,160

References: (a) Luckiesh, Matthew (1946) Application of Germicidal, Ethyemal and Infrared Energy,

D. Van Nostrand o., New York, New York, pp 253 (b) Hollaender (1942) Aerobiology, A.A.A.S. (for 90% inactivation), pp 162 (c) Ellis, C. and Wells, O.O. (1941) The Chemical Action of Ultraviolet Rays, Reinhold Publishing Corp.,

pp. 713-714 (d) Hollaender, A., Oliphant, J.W. (1944) The Inactivation Effect of Monochromatic Ultraviolet. Radiation

on Influenze Virus (for 90% inactivation) Jour. of Bact. 48, pp. 447-454 (e) This table, "Energies Required to Destroy Some Microorganisms by Ultraviolet Radiations" comes from

Westinghouse brochure entitled - "Westinghouse Sterilamp Germicidal Ultraviolet Tubes"

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NOTE:

11.0 Electrical/Environmental Requirements 11.1 Electrical: (Supply voltage fluctuations not to exceed +/- 10%)

*NU-602-4XX 115 VAC, 60 Hz, 1 Phase, 13 Amps *NU-602-5XX 115 VAC, 60 Hz, 1 Phase, 15 Amps *NU-602-6XX 115 VAC, 60 Hz, 1 Phase, 15 Amps **NU-602-4XXE 230 VAC, 50 Hz, 1 Phase, 9 Amps **NU-602-5XXE 230 VAC, 50 Hz, 1 Phase, 11 Amps **NU-602-6XXE 230 VAC, 50 Hz, 1 Phase, 11 Amps

*UL/UL-C Listed **CE Certified 11.2 Operational Performance (for indoor use only)

Environment Temperature Range: 60°F - 90°F (15.6°C - 32.2°C) Environment Humidity: 20% - 60% Relative Humidity

Environment Altitude: 6562 Feet (2000 Meters) Maximum 11.3 Light Exposure

Standard Fluorescent Lighting @ 150 ft. candles (1614 LUX) maximum intensity. 11.4 Installation Category: 2.0

Installation category (overvoltage category) defines the level of transient overvoltage, which the instrument is designed to withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example, in CAT II, which is the category used for instruments in installations supplied from a supply comparable to public means, such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply.

11.5 Pollution Degree: 2.0

Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation.

11.6 Chemical Exposure

Chemical exposure should be limited to antibacterial materials used for cleaning and disinfecting. Chlorinated and Halogen materials are not recommended for use on stainless steel surfaces. Chamber decontamination can be accomplished by paraformaldehyde, vapor phased Hydrogen Peroxide or Ethylene Oxide without degradation of cabinet materials.

11.7 EMC Performance (classified for light industrial)

Emissions: EN61326 Immunity: EN61326 The EMC performance requirements are generated within the product enclosure. The enclosure will be all metal grounded to earth. In addition, the membrane front panel will also include a ground plane for maximum protection and an electrostatic shield.

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CAUTION

NOTE:

12.0 Disposal and Recycle

Cabinets that are no longer in use and are ready for disposal contain reusable materials. ALL components with the exception of the HEPA filters may be disposed and/or recycled after they are known to be properly disinfected. Follow all local, state and federal guidelines for disposal of HEPA filter solid waste.

Prior to any disassembly for disposal, the cabinet must be decontaminated.

Component Material Main Cabinet (Top) Stainless Steel Main Cabinet (Bottom) Painted Steel Front Grill Stainless Steel Worksurface Stainless Steel Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Control Center Painted Steel Supply Diffuser Aluminum Exhaust Filter Aluminum HEPA Filter Frames Painted Steel Hepex Bag PVC Blower Wheel & Housing Steel Motor Various Steel/Copper Printed Wiring Assembly Lead Free Electronic Wire PVC Coated Copper Ballasts Various Steel, Electronic Connectors Nylon Hardware Stainless Steel and Steel Lift Motor Various Steel /Copper Castors Stainless Steel Lift System Base Plate Stainless Steel Lift System Glides Plated Steel

Material type can be verified with use of a magnet with stainless and aluminum being non-magnetic.

NOTE:

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BIOHAZARD

RECYCLE

LEAD FREE

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