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ratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

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Page 1: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

Artemia research at ARC

Page 2: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

1. ARC ‘traditionals’: description of strain characteristics

• biometrics• hatching & diapause characteristics• lifespan & reproductive characteristics• effect of abiotic conditions on growth, reproduction….• cross-breeding• HUFA content• ……..

cooperation with other partners !

Page 3: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

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2. Artemia population assessment studies

cfr. fluctuations in GreatSalt Lake harvests

• abiotic conditions• primary production• Artemia population

Page 4: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

Karabogaz-Gol, Turkmenistan

Russian Federation(Siberia)

China (Tibet)

Page 5: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

Orius

Podisus

3. Mass-rearing and biocontrol potential of predatory insects (Heteroptera, Coleoptera…) (Cooperation with Laboratory of Agrozoology, RUG)

Harmonia

Page 6: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

nymph IV Orius

Cost-effective production of ‘killer bugs’ : production of large numbers at low price

Use of Lepidopteran eggs, esp. of Mediterranean flour moth, Ephestia kuhniella

• good development & reproduction of predatory insects

• high price (high investment & production costs, high demand, occasional market shortages)

• possible human health problems (allergene)

Page 7: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

Promising preliminary results : Artemia cysts as alternative to Ephestia eggs

effect on growth, metamorphosis, survival, reproduction of predator…

application techniques (decapsulated vs. non-decapsulated, hydration level…)

Page 8: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

4. Proposal for a new VIB (Flemish Institute of Biotechnology) Department : Department of Microbial Ecology of Aquatic Invertebrates

Ghent University, Belgium• Laboratory of Microbial Ecology and Technology• Laboratory of Aquaculture & Artemia Reference Center

University of Connecticut, USADalhousie University, Canada

 

Page 9: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

Rationale :Eukaryotes exist in close association with micro-organisms: symbiotic, pathogenic, neutral= EMCs (eukaryote microbial communities) Colonisation of EMCs by allochthonous organisms = 1st step in development of pathogenesis, commensalism, symbiosis.

Objective : How do allochthonous micro-organisms establish themselves in the EMCs ? What factors determine success or failure ?

new insights into host-pathogen and host-probiotic interactions therapeutic and probiotic opportunities.

Model eukaryote: Artemia franciscana Model allochthonous organisms: Vibrionaceae

• pathogenic or beneficial to Artemia• culture collection available; extensive database available of chromosomal & mobile genetic elements important to Vibrio success in EMCs • common pathogens of almost all higher eukaryotes,

develop a comprehensive model of Vibrionaceae invasion and colonisation

Page 10: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

Host-Microbe Unit

Page 11: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

Microbe-Microbe Unit

Page 12: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

Mobile Genetic Element Unit

Page 13: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

5. FWO project: the functional role and characteristics of micro-organisms in the larviculture of aquatic organisms with Artemia as a test species

Rationale :

• general trend towards more natural suppression of opportunistic/obligate pathogens in aquaculture• consensus about critical role of microbial interactions

• numerous questions about biological processes involved :competition for adhesion sites in digestive tractcompetition for nutrientsexcretion of antibacterial products by bacteria/micro-algaestimulation of immune system by algae/yeasts/bacteria………

• test organisms not cultured axenically interactions with micro-organisms

Page 14: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

opportunistic bacteria

pathogenic bacteria

probiotic bacteria

Artemia as model organism

partners: • Laboratory of Aquaculture & Artemia Reference Center (RUG)• Prof. Godelieve Criel (RUG)• Laboratory for Microbial Ecology (RUG)• Laboratory for Aquatic Ecology & Aquaculture (KULeuven)

Objective: Study the mode of action of microbial organisms & verify hypotheses byuse of axenically cultured organism

Page 15: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

research questions: 1. How do bacteria colonize the gut??

Page 16: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

ingestion?

through the cuticula?

through the gills?

research questions: 1. How do bacteria colonize the gut?2. How does a pathogen infect an aquatic organism?

Page 17: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

research questions: 1. How do bacteria colonize the gut?2. How does a pathogen infect an aquatic organism?3. How does a ‘probiotic’ protect an aquatic organism?

Page 18: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

First action: develop an axenic standard culture of Artemia

autoclaved

decapsulated

-irradiated

culture vessels

food

cysts

seawater

Page 19: Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium Artemia research at ARC

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

Further actions:

Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium

• Linked to the axenic cultures and using scanning-electron microscopy, an ‘atlas’ of a ‘normal’ gut of successive developmental stages would be compiled.

• At a later stage, the colonization of the gut by selected harmless and pathogenic bacteria will be studied quantitatively and qualitatively using diverse microbial marker techniques (FiSH, DGE, GFP).

• Besides, we will consider axenic Dunaliella cultures as food source in order to investigate the possible probiotic role of algae.