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microTec® Laborgeräte GmbH Company for manufacture and distribution of laboratory equipment 1

Mikrotom-XL

Large Sliding Microtome

User Manual


Table of Contents

Chapter1………………………………………. ..................................................................................................................... 5

1. Basic Instructions………………. ..................................................................................................................... 6

1.1 Significance of the User Manual ............................................................................................................................. 6

1.2 Documents .............................................................................................................................................................. 7

1.2.1 Certificate of Warranty .................................................................................................................................... 7

1.2.2 Commissioning report / acceptance report / instruction report ..................................................................... 8

1.2.3 Repair history ................................................................................................................................................. 10

1.2.4 Declaration of Conformity ............................................................................................................................. 11

1.3 Approved Use ........................................................................................................................................................ 12

1.4 Non-approved Use ................................................................................................................................................ 12

1.5 Warranty and Liability ........................................................................................................................................... 13

1.6 Presentation of Use, Functions, and Operating Process ....................................................................................... 14

1.7 Edition, Issue Date of the User Manual ................................................................................................................ 15

1.8 Copyright ............................................................................................................................................................... 15

1.9 The Manufacturer’s Address ................................................................................................................................. 15

Chapter2………………………………………. ................................................................................................................... 16

2. Basic Safety Instructions…….. .................................................................................................................. 17

2.1Please Observe these Instructions! ....................................................................................................................... 17

2.2 The Operator’s Obligations ................................................................................................................................... 17

2.3 The Personnel’s Obligations .................................................................................................................................. 17

2.4 Potential Hazards when Handling the Device ....................................................................................................... 18

2.5 Explanation of Hazard Symbols ............................................................................................................................. 20

2.6 Useful Instructions from the Manufacturer .......................................................................................................... 21

2.7 Particularly Dangerous Places ............................................................................................................................... 22

2.8 Built-in Safety Features ......................................................................................................................................... 22

2.9 Maintenance and Troubleshooting ....................................................................................................................... 22

2.10 Structural Modifications of the Device ............................................................................................................... 23

2.11 Cleaning and Upkeep .......................................................................................................................................... 23

Chapter3………………………………………. ................................................................................................................... 24

3. Transport and Storage…………. ................................................................................................................. 25

3.1 Dimensions and Weight ........................................................................................................................................ 25

3.2 Instructions for Transport ..................................................................................................................................... 25

3.3 Packing .................................................................................................................................................................. 25

3.4 Acceptance Inspection by Recipient ..................................................................................................................... 25

3.5 Transport Damage Reporting and Required Documentation ............................................................................... 26


3.6 Ambient Conditions .............................................................................................................................................. 26

Chapter4………………………………………. ................................................................................................................... 27

4. Device Data……………………….. ................................................................................................................... 28

4.1 Name ..................................................................................................................................................................... 28

4.2 Delivery Scope ....................................................................................................................................................... 28

4.3 Ambient Conditions on Operating Site ................................................................................................................. 29

4.4 Technical Data / Performance .............................................................................................................................. 29

Chapter5………………………………………. ................................................................................................................... 30

5. Installation, Assembly, Commissioning .................................................................................................... 31

5.1 Installation ............................................................................................................................................................ 31

5.2 Unpacking/Transport Security Measures ............................................................................................................. 31

5.3 Connecting the Device .......................................................................................................................................... 32

5.4 Assembly of the Accessories ................................................................................................................................. 33

5.4.1 Assembling the Knife Fixture ......................................................................................................................... 33

5.5Device Components, Operating and Display ......................................................................................................... 34

5.6 Instructions re Operating Elements ...................................................................................................................... 35

Chapter6………………………………………. ................................................................................................................... 36

6. Touchscreen, Operating Functions ........................................................................................................... 37

6.1 Turning on the Device ........................................................................................................................................... 37

6.2 Touchscreen .......................................................................................................................................................... 38

6.3 Basic Screen .......................................................................................................................................................... 39

6.4 OM-Setting ............................................................................................................................................................ 40

6.4.1. Tap Setting .................................................................................................................................................... 40

6.4.2. Manual Dial Setting ....................................................................................................................................... 41

6.4.3. Changing Position .......................................................................................................................................... 42

6.5 OM-Automatic ...................................................................................................................................................... 43

6.5.1. Display of Default Values .............................................................................................................................. 44

6.5.2. Display of Actual Values ................................................................................................................................ 45

6.5.3 Selecting the Stroke Type ............................................................................................................................... 45

6.5.4. Cutting Functions .......................................................................................................................................... 49

6.5.5. Preselecting Stop Positions ........................................................................................................................... 51

6.5.6. Trimming ....................................................................................................................................................... 53

6.5.7. Sectioning Way .............................................................................................................................................. 54

6.6 Touchscreen Calibration ....................................................................................................................................... 55

6.7 Screen Cleaning ..................................................................................................................................................... 55

6.8 Info ........................................................................................................................................................................ 56

6.9 Fault Reporting / Troubleshooting ........................................................................................................................ 56


6.10 Parameters ...................................................................................................................................................... 58

Chapter7………………………………………. ................................................................................................................... 59

7. Operation………………………….. ................................................................................................................... 60

7.1 Sample Fixing ........................................................................................................................................................ 60

7.2 Knife Fitting or Knife Type Changing ..................................................................................................................... 60

7.2.1 Polycut knife Typ 1 + 2 ................................................................................................................................... 61

7.2.2 Universal Knife holder .................................................................................................................................... 63

7.3 Define Default Values /Setting .............................................................................................................................. 64

7.4 Knife Angle Setting ................................................................................................................................................ 65

7.4.1 Polycut Knife holder, Type 1 + 2 .................................................................................................................... 65


7.5 Declination Angle Setting ...................................................................................................................................... 67

7.5.1 Polycut Knife holder (Type 1 + 2) ................................................................................................................... 67


7.6 Section Thickness and Section Number Setting .................................................................................................... 68

7.7 Sectioning Window ............................................................................................................................................... 68

7.8 Sectioning .............................................................................................................................................................. 69

7.9 Select Sectioning Speed ........................................................................................................................................ 71

7.10. Taking off sections ............................................................................................................................................. 72

Chapter8………………………………………. ................................................................................................................... 73

8. Fault Reporting / Troubleshooting ........................................................................................................... 74

Chapter9………………………………………. ................................................................................................................... 75

9. Accessories………………………………. ................................................................................................................... 76

9.1 Accessories to the Knife/Knife holder ................................................................................................................... 76

9.2 Object Fixation and Positioning Accessories ......................................................................................................... 79

9.3 Table accessory ..................................................................................................................................................... 83

Chapter10……………………………………… ................................................................................................................... 84

10. Disposal of waste…………………….. ............................................................................................................. 85

Chapter11…………………………………….. .................................................................................................................. .86

11. Emergency Service, Customer Service .......................................................................................................... 87

11.1 Address................................................................................................................................................................ 87

11.2 Instruction for Emergency and Customer Services ............................................................................................. 87

Chapter12…………………………………….. ................................................................................................................... 88

12. List of Figures/Illustrations………… ........................................................................................................... 89

Chapter13…………………………………….. .................................................................................................................. .90

13. Notes………………………………………. ............................................................................................................. 91


Chapter1

Basic Instructions

1.Basic Instructions ............................................................................................... 6

1.1 Significance of the User Manual ............................................................................ 6

1.2 Documents ............................................................................................................. 7

1.2.1 Certificate of Warranty. ...................................................................................... 7

1.2.2 Commissioning report / acceptance report / instruction report. ....................... 8

1.2.3 Repair history. ................................................................................................... 10

1.2.4 Declaration of Conformity. ................................................................................ 11

1.3 Approved Use ....................................................................................................... 12

1.4 Non-approved Use ............................................................................................... 12

1.5 Warranty and Liability .......................................................................................... 13

1.6 Presentation of Use, Functions, and Operating Process ...................................... 14

1.7 Edition, Issue Date of the User Manual................................................................ 15

1.8 Copyright .............................................................................................................. 15

1.9 The Manufacturer’s Address ................................................................................ 15


1. Basic Instructions

1.1 Significance of the User Manual

Dear Customers,

Please read the User Manual thoroughly before the commissioning of the appliance and

make sure that you become acquainted with the functions and user elements, as it is

imperative to ensure proper handling of the device.

Please compare the machine number with the number on the microTec type label of your

device.

Inquiries and service can be simplified, if you always quote the machine number.

microTec– machine number: -

microTec Laborgeräte GmbH

Rudolf-Diesel-Str. 47 69190 Walldorf, Germany Phone: + 49 (0) 6227 / 8222-0 Fax: + 49 (0) 6227 / 8222-30 [email protected] www.micro-tec.de

mailto:[email protected]


1.2 Documents

1.2.1 Certificate of Warranty

Certificate of Warranty

microTec Laborgeräte GmbH hereby grants the legal guarantee in accordance with german law.

The warranty period begins after commissioning and instruction of the device.

The dealer must pass on a commissioning record, acceptance report and the warranty certificate to the

manufacturer. Within the guarantee, microTec Laborgeräte GmbH provides the spare parts free

of charge.

Customer: __________________________________________________________________

Contact person: _____________________________________________________________

Street / No.: ________________________________________________________________

Town / zip code: _____________________________________________________________

Model: _____________________________________________________________________

Serial number: _______________________________________________________________

Distributor: __________________________________________________________________

In order to assert warranty claims, the microtome must be displayed at the authorized dealer where the

device was purchased. The original invoice with the date and the warranty certificate must be enclosed.

Warranty and liability claims for personal injury or material damage are excluded if these are attributable to

one or more of the following causes:

Non-intended use of the device

Incorrect installation, commissioning, operation and maintance of the device

Operate the device in the case of defective, improperly installed or non-functional safety and protective

devices

Do not pay attention to the instructions in the operating instructions regarding transport, storage,

installation, commissioning, operation, maintenance and setting up of the device

Unauthorized structural modification of pneumatic, electrical and mechanical parts

Long-term monitoring of machine parts subject to wear

Improper repairs performed

Disasters caused by contaminant and force majeure


1.2.2 Commissioning report / acceptance report / instruction report

Customer name: Distributor:

Contact person: Service-

engineer:

Email:

Departmend:

Street / No.:

Town: Zip code:

Country:

Model: Serial number:

Year of

construction: Software version:

Have there been any defects during commissioning?

Yes: No: If yes, which?

Has the customer been trained on the machine?

Yes: No: If no, why?

Names:


Were there missing parts?

Yes: No: If yes, which?

Were there problems with the installation?

Yes: No: If no, why?

Notes:

The microtome has been installed by an proffesional:

Date / Signature / Stamp

Customer:

Date / Signature / Stamp

Service- engineer:


1.2.3 Repair history

Model: Machine – No.:

Problem: Task: Responsible: Date: Status:


1.2.4 Declaration of Conformity

EU Declaration of Conformity

Pursuant to EC Machine Directive 2006/42/EC, Annex II, 1.A

Manufacturer: microTec Laborgeräte GmbH Rudolf-Diesel-Str. 47 D-69190 Walldorf, Germany

Product:

Serial Number:

Person in the Community duly authorized to compile the relevant technical documentation: Walter Ganter – microTec Laborgeräte GmbH Walldorf, 11.11.2015

Place, Date Walter Ganter, managing director

It is declared, bearing sole responsibility, that the product meets the abovenamed Directive and the

subsequent harmonizing standards applied.

2006/42 /EC:2006-05-17 2006/42 /EC: EU Machine Directive 2006/42 /EC 2006/95 /EC: (Low Voltage Directive) Directive of the European Parliament and Council of 12 December 2006 on the

approximation of the Member States‘ laws concerning electrical equipment designated for use within certain voltage limits (Codified Version) (1)

2004/108 /EC: (Electromagnetic Compatibility) Directive of the European Parliament and Council of 15 December 2004 on the approximation of the Member States’ laws concerning electromagnetic compatibility and repealing Directive 89/336/EEC

2006/42 /EC: (Machine Directive) Directive 2006/42/EC of the European Parliament and Council of 17 May 2006 on machines and amending Directive 95/16 /EC (New Version) (1)

References to harmonized standards applied:

EN ISO 13849-1:2008 Machinery Safety – Safety-relevant control components - Part 1: General Design Principles (ISO 138491:2006)

EN 62061:2005 Machinery Safety – Functional safety of safety-relevant electrical, electronic, and programmable electronic control systems

EN ISO 13857:2008 Machinery Safety – Safety distances to prevent hazardous zones being reached by upper and lower limbs (ISO 13857:2008)

EN 60204-1:2006-06 Machinery Safety – Electrical Equipment of Machines - Part 1: General Requirements EN ISO 14121-1:2007 Machinery Safety – Risk Assessment - Part 1: Guiding Principles (ISO 14121-1:2007)

Apart from the above, company standards compliant with DIN EN ISO 9001:2008 have been applied.

Mikrotom-XL / Microtome for Cutting Large and Hard Slices

-


1.3 Approved Use

Mikrotom-XL is an electronically operated large microtome slicer designated for work in

paraffin sectioning, hard sectioning, and material sciences in the fields of biology,

medicine, and industry.

The sectioning range can be set up between 0 – 1.000 µm in 1 µm steps.

The user-friendly disposable blades and universal knife holder with integrated knife guard

ensure problem-free clamping and adjusting of the knives.

Mikrotom-XL is fitted with an integrated sectioning counter that shows the number of

performed sections on the operator’s display panel.

In order to prevent damage to the knife or the sample, Mikrotom-XL is provided with a

knife retraction function during the test, which may be set liberally between 0 – 1.000µm.

Three-stage trimming function (10µm, 20µm and 30µm) is another built-in function.

The sectioning range is liberally adjustable and can be limited by a teach function (reset).

The device can be controlled optionally in single stroke, continuous stroke, touch stroke,

or pre-dial stroke mode using the control unit or foot switch.

A two-stage manual method with the knife, as well as a one-stage method with the

sample is possible.

1.4 Non-approved Use

Mikrotom-XL is designed and built in accordance with present-day technology and

recognized regulations of technical safety. Nevertheless, personal injury to the user or

third parties, or impairment to the device, software, or other material values may still

occur. Therefore, the device is only to be used:

for designated purposes

in a technically safe and perfect condition


1.5 Warranty and Liability

The company microTec GmbH confirms that the device has been carefully tested and

reexamined. The conformity of the published data was verified prior to delivery of the

device.

The device is classified as Med GV Class 4. Compliance with current regulations is ensured.

This microTec product is covered against any defects of material or workmanship under a

warranty for 12 months following purchase, free of charge. During the warranty period,

defective parts will be repaired or replaced. Any further claims are precluded. Defects

caused by a third-party intervention are not covered by the warranty.

In order to file a valid warranty claim, Mikrotom-XL must be presented to the authorized

dealer, where the device was bought, along with the original receipt, dated, the purchase

note, and the Warranty Certificate.

In general, all claims are subject to our General Purchase and Delivery Conditions that will

be available to the operator at the latest upon signature of the purchase contract. In

cases of personal or material injury, warranty and liability claims are precluded, if one or

multiple causes of the injury/injuries is/are consequential to:

unapproved use of the device!

Unprofessional installation, commissioning, operation, and/or maintenance of the device;

operation of the device using defective, improperly installed, or dysfunctional safety and/or protective facilities;

non-observance of the instructions in the User Manual concerning transport, storage, installation, commissioning, operation, maintenance, and setup of the device;

unauthorized structural modification of pneumatic, electrical, and/or mechanical parts;

inadequate monitoring of the machine components that are prone to wearing;

improperly done repairs;

disasters caused by the impact of alien particles and/or vis major.


1.6 Presentation of Use, Functions, and Operating Process

Designated Use Mikrotom-XL is a fully automated device designated for all types of work using paraffin

and hard-sectioning technology and material sciences in the fields of biology, medicine,

and industry.

The Functions and Operating Process Mikrotom-XL operates on the principle of rotary motion of an engine driven by belts that

transpose the motion into vertical sample sectioning. The specimen are sectioned by

regrindable knives (sharpening) or disposable blades adapted to and held by various

clamps and holders.

The sectioning motion is used for sectioning the object. If selecting the continuous mode,

the section is done up to a pre-defined end point, where knife retraction follows and

drives the knife by a selected value upwards. Thereupon, the knife returns to the starting

point in front. This is where the thickness of the specimen is selected and the sectioning

motion restarts.

In the single-stroke mode, the object sectioning stops at the rear or front point, as

selected.

The single-stroke, continuous-stroke, as well as the touch-stroke may be controlled both

from the operator panel or the foot switch.

The device has a three-stage trimming function, which facilitates quick approximation of

the sample.

In order to be able to accommodate different types of objects, the device is provided with

a multifunction object clamping system. Object positioning allows simple alignment to the

sectioning plane possible.

The different knife holders ensure a problem-free fitting with various types of knives and

proper use-specific setting.


1.7 Edition, Issue Date of the User Manual

Edition: Version 001.16 Date of Issue: 13.06.2016

1.8 Copyright

Copyright: microTec Laborgeräte GmbH

The copyright to this User Manual remains the property of microTec Laborgeräte GmbH.

The User Manual is intended to be used solely by the operator and its personnel.

It contains regulations and instructions that may not be, completely or partly:

- duplicated,

- distributed,

- or otherwise disclosed to other parties.

Violations could be legally prosecuted.

1.9 The Manufacturer’s Address


Rudolf-Diesel-Str. 47 69190 Walldorf, Germany Phone: + 49 (0) 6227 / 8222-0 Fax: + 49 (0) 6227 / 8222-30 [email protected] www.micro-tec.de



Chapter2

Basic Safety Instructions

2. Basic Safety Instructions .................................................................................. 17

2.1 Please Observe these Instructions! ................................................................... 17

2.2 The Operator’s Obligations ................................................................................ 17

2.3 The Personnel’s Obligations .............................................................................. 17

2.4 Potential Hazards when Handling the Device ................................................... 18

2.5 Explanation of Hazard Symbols ......................................................................... 20

2.6 Useful Instructions from the Manufacturer ..................................................... 21

2.7 Particularly Dangerous Places ........................................................................... 22

2.8 Built-in Safety Features ...................................................................................... 22

2.9 Maintenance and Troubleshooting ................................................................... 22

2.10 Structural Modifications of the Device ............................................................ 23

2.11 Cleaning and Upkeep ....................................................................................... 23


2. Basic Safety Instructions

2.1Please Observe these Instructions!

A basic prerequisite to safe handling and trouble-free operation of the device is the

knowledge of the basic safety instructions and safety regulations.

This User Manual contains important instructions how to operate the device safely.

This User Manual, particularly the safety instructions in it, are to be observed by all persons

who work with the device.

Moreover, it is imperative to follow all the locally applicable rules and regulations for

accident prevention.

2.2 The Operator’s Obligations

The Operator undertakes to entrust the operation of the device exclusively to persons who

are acquainted with the basic regulations of work safety and accident prevention,

and instructed about the handling of the device;

have read and understood the chapter on safety and the warnings in this User

Manual;

have been taught to approach work processes conscientiously.

2.3 The Personnel’s Obligations

All persons assigned to work with the device, before they start to work, assume the

following obligation:

to observe the basic work safety and accident prevention regulations;

to make sure that they read and comprehend the chapter on safety and the

warnings in this User Manual.


2.4 Potential Hazards when Handling the Device

Caution!

The following safety measures must be observed

whenever using the device. Failure to follow these

precautions would constitute a violation of recognized

technical rules as well as appropriate use of the device.

microTec Laborgeräte GmbH precludes liability for any

infringements or violations of these precautions.

Danger of Injury

When setting up the knife and object, the worker should

be wearing finger protection as well as gloves as

protection against cuts, in order to minimize the risk of

injury by the knife or blade.

Always fasten the object first, then set the knife in to the

knife holder.

Before changing the clamping system, always take out the

knife/blade first. Knives that are not being used should

always be properly safeguarded in a knife box. Never try

to catch a falling knife in midair. Be careful during the

sectioning process and while taking off the section.

Risk of an Infection

When working with infectious material, always wear

proper protection and apply disinfection measures.


Risk of Radiation

When working with radioactive materials or objects,

always observe the relevant anti-radiation regulations.

Risk of Splinters

When sectioning brittle samples, there exists potential

danger of splinters – therefore, always wear protective

glasses.

Risk of Magnetic Fields

Persons wearing a pacemaker may not perform work where

a magnet is used as a means of fastening.

General Safety Instructions and Warnings

During the sectioning processes, make sure that no liquid

penetrates into the device.

Whenever the sectioning process is interrupted, close the

finger guard.

During cleaning and maintenance work, the device may

only be opened by authorized personnel.

Before cleaning, the device must be turned off and

disconnected from power. (Touchscreen may be cleaned

while turned on).

The operator panel must be turned off before cleaning.

Do not clean with solvents or abrasive cleaners.

Use appropriate cleaning detergents for the operator’s

panel and non-lacquered parts. Use only slightly moistened

cloth for cleaning.


2.5 Explanation of Hazard Symbols

Signalword

Pictogram

- Danger

- Warning

- Caution

- Note

- Important!

- Information


2.6 Useful Instructions from the Manufacturer

Object Preparation

When preparing objects for sectioning, care must be taken to select the correct

embedding medium, as well as the time of fixation and infiltration.

Object Temperature

As a rule, sectioning is done at room temperature (frozen materials excepting). Paraffin

softens at higher temperature, therefore avoid working in direct sunlight or near any

source of heat.

Instructions for Setting the Knife

Depending on the facet cut, angle setting of approx.10-15° is recommended. When using

a sharpened knife, the height of the knife must be adjusted accordingly.

Lever and Clamping Screws

All lever and clamping screws on the knife fixture and object position must be firmly

tightened.

Sectioning Speed

Note: «The harder the material, the slower the sectioning speed».

Starting to Section

Be careful when approaching the knife and/or object.


2.7 Particularly Dangerous Places

CAUTION !

- Microtome knives are very sharp.

After ending or interrupting work, even if for a

short time, it is imperative to apply the knife

guard or take the knife out and put away safely.

- The device is to be placed in a safe place.

Mikrotom-XL can roll away – therefore, the brakes must be activated at all

times, as they lift Mikrotom-XL and take the weight off the rollers, so that

position shifting is not possible.

2.8 Built-in Safety Features

- Emergency button on the operator’s panel

- Emergency button on the foot switch, integrated

- Finger guard over the knife

- Overloading control of the engine

2.9 Maintenance and Troubleshooting

Maintenance

In order to ensure the quality of cutting and safe functionality of the device, a routine

maintenance should be performed annually. Maintenance tasks may only be carried out

by trained service technicians.

Maintenance Contract

microTec offers a maintenance contract which guarantees that your device will be in

perfect condition.

Troubleshooting

Malfunctions affecting safety are to be rectified and/or reported immediately.


2.10 Structural Modifications of the Device

- No modifications, additions, or reconstructions may be made without the

manufacturer’s permission

- All modification measures require a confirmation/release of microTec in writing.

- Machine parts that are not in perfect condition are to be replaced immediately.

- Only original spare parts and consumables may be used.

In the event that non-original parts are used, it cannot be guaranteed that they meet the relevant manufacturing and safety requirements.

2.11 Cleaning and Upkeep

Cleaning

Depending on the degree of contamination and frequency of use, it is recommended that

the device be cleaned regularly using mild household detergents. Aggressive cleaning

materials or solvents may not be used, as they could damage the surface of lacquered or

plastic parts. It is advised to move the device into the changing position for cleaning.

Always take the knife out and put it in the knife box, before

starting the cleaning process.

Caution!

Never put the knife on the desk with the cutting edge up. The

contact surfaces as well as the knife guiding carriers should be

cleaned particularly thoroughly.

Upkeep

When the device is not being used, move the cutter into the „changing“ position and close

the cover that is enclosed in the delivery, then use the main switch to turn the device off.


Chapter3

Transport and Storage

3. Transport and Storage ..................................................................................... 25

3.1 Dimensions and Weight ..................................................................................... 25

3.2 Instructions for Transport .................................................................................. 25

3.3 Packing ............................................................................................................... 25

3.4 Acceptance Inspection by Recipient .................................................................. 25

3.5 Transport Damage Reporting and Required Documentation ........................... 26

3.6 Ambient Conditions ........................................................................................... 26


3. Transport and Storage

3.1 Dimensions and Weight

Dimensions in mm:

Microtome (HxWxL): ................................................................................ 400x480x810

Control unit/panel (HxWxL):……………………..…….…150x470x400 plus telescope mounting

Microtome main frame (HxWxL): ........................................ .……………….. 995x665x1175

Required minimum space for the Microtome (HxWxL):…………….…….1.500x2.000x1.600

Weight in kg:

Weight (without accessories):…………………………………………………………………………..550 kg

3.2 Instructions for Transport

Remove all knives, the knife box, object carrier, and objects from Mikrotom-XL. Bring the

operator’s panel centrally over the microtome to a convenient transport situation. When

lifting the microtome box, hold it only by the specially marked points for lifting.

3.3 Packing

The microtome is delivered in a specially lined wooden box. Mikrotom-XL may be only be put

into operation after 24 hours following delivery.

3.4 Acceptance Inspection by Recipient

Check, whether all the parts ordered are contained in the transport box. Check the transport

box itself and the microtome for any transport damage.


3.5 Transport Damage Reporting and Required Documentation

Any and all transport damage is to be reported to the manufacturer immediately and the

transport company notified.

3.6 Ambient Conditions

- Storage temperature -20°C to + 50°C

- Not to be stored in open areas; protect from rain or moisture.


Chapter4

Device Data

4. Device Data .................................................................................................... 28

4.1 Name.................................................................................................................. 28

4.2 Delivery Scope ................................................................................................... 28

4.3 Ambient Conditions on Operating Site .............................................................. 29

4.4 Technical Data / Performance ........................................................................... 29


4. Device Data

4.1 Name

Mikrotom-XL

4.2 Delivery Scope

as per Contract


4.3 Ambient Conditions on Operating Site

- 18 °C to + 35°C

- Relative air humidity up to 60%, maximum, no condensation.

4.4 Technical Data / Performance

Mikrotom-XL

Sectioning thickness range: 0µm to 1.000µm, adjustable by µm Total sample feed: max. 350 mm Total vertical stroke of the knife: max. 70 mm Knife retraction (during sample processing): liberally adjustable 0 µm to 1.000 µm Wide angle setting: 0° to 30° Knife declination: liberally adjustable 0° to 80° (with adjustable knife fixture) Maximum sample size (LxWxH): 300x200x70 mm Sample orientation on X/Y axis: 5°

Sample orientation on Z axis: 360° (by means of a magnet clamp) Cutting speed: 1 mm/s to 125 mm/s, adjustable by 1mm Processing speed: 1 mm/s to 125 mm/s, adjustable by 1mm Cutting window: liberally adjustable Manual mode of the knife: in two stage Manual mode of the sample: in one stage Electrical Connections Nominal voltage: 400 V Nominal frequency: 50 Hz Power: 1,5 kW Nominal current: 6,3 A Total power consumption: max. 1,500 VA Fuse: 16A

(Technical data subject to change without notice)


Chapter5

Installation, Assembly, Commissioning

5. Installation, Assembly,Commissioning ............................................................. 31

5.1 Installation ......................................................................................................... 31

5.2 Unpacking/Transport Security ........................................................................... 31

5.3 Connecting ......................................................................................................... 32

5.4 Assembly of Accessories .................................................................................... 33

5.4.1Knife holder Assembly ..................................................................................... 33

5.5 Device Components, Operating and Display Elements ..................................... 34

5.6 Instruction to Operating Elements .................................................................... 35


5. Installation, Assembly, Commissioning

5.1 Installation

Protect the device from: - Sources of heat, such as heating fans or hot-air fans - Extremely low temperatures - Direct sunlight - Very dusty environment - Moisture or rain - Mechanical vibrations or bumping - Devices containing magnets or producing magnetic fields Installation Conditions: - Space for placing the microtome and operator’s panel of at least 1.400 x 2.000 x 1.600 mm - At least 150mm free space around the device for air circulation - Virtually vibration-free floor - Room temperature steadily between + 18°C to + 35°C - Relative air humidity of up to 60%, maximum, condensation-free.

5.2 Unpacking/Transport Security Measures

Lift the box solely by the points specially marked for lifting. Open the transport box and

remove all transport security elements, adhesive tapes, and protective foil. Prop the

device on the rollers and roll it carefully down the ramp.

CAUTION! The device is very heavy. There is a risk of injury or crushing, if the device is being rolled without the possibility of braking.


2x 230V sockets Main dialswitch

5.3 Connecting the Device

Note: The device may only be connected to a VDE protected contact socket! Verify that

the voltage and power are correct. Check, whether the main dial switch is in the OFF

position. Plug the power plug into a 400V socket. Plug the optional lighting into one of the

two 230V sockets (on the rear).


Screws

Fig. 1 Accessories

5.4 Assembly of the Accessories

5.4.1 Assembling the Knife Fixture

Unscrew the 2 threaded studs and set the knife holder from the front onto the guiding

carriers and then tighten the T-nut with the 4 screws.


Fig. 2 Mikrotom-XL

5.5Device Components, Operating and Display

1. Mikrotom-XL

1.1 Sample carrier

1.2 Knife carrier/Guiding

1.3 Object positioning

1.4 Lateral knife adjustment

1.5 Knife holder

1.6 Finger guard

2. Optional magnifying glass w/lighting

3. Operator’s panel

3.1 Touchscreen

3.2 Manual knife carrier dial

3.3 Manual object carrier dial

3.4 Potentiometer

sectioning speed

3.5 Emergency button

3.6 Ready-for-operation lights

4. Foot switch

5. Heavy-duty rollers

1.

3.6

3.5

3.4

3.3

3.2

3.1

3.

2.

1.6

1.5

1.4

1.3

5.

4.

1.2

1.1


Fig. 3 Foot switch Fig. 4 Potentiometer & manual dials

3.2 3.4 Potentiometer

5.6 Instructions re Operating Elements

- Ad 3.2.and 3.3. manual dials: The manual dials are only active in the window dial

(6.4.2), where they can be switched to the different time carriers of the grid.

- Ad 3.4 potentiometer: The potentiometer is used for adjusting the cutting speed.

When the potentiometer is set at Zero, the object carrier cannot be moved, until the

potentiometer is turned to the desired value.

- Ad 4. foot switch. The foot switch can be activated/deactivated using touchscreen,

where OM-Automatic and all stroke types may be started. Firm pressure on the foot

switch will activate the emergency function and the reason for problem reporting is

to be acknowledged (6.9). Before acknowledgement, it is necessary to press the blue

button on the foot switch.

- Ad 5. heavy-duty rollers: The heavy-duty rollers are used for moving the device.

During the cutting process and after positioning the device in its location, the built-in

brakes must be used to prevent the device from rolling and the support footing set as

described in in Chapter 2.7.


Chapter6

Touchscreen, Operating Functions

6. Touchscreen, Operating Functions ................................................................... 37

6.1 Turning on the Device ........................................................................................ 37

6.2 Touchscreen ...................................................................................................... 38

6.3 Basic Screen ....................................................................................................... 39

6.4 OM-Setting ......................................................................................................... 40

6.4.1. Setting Tapping .......................................................................................... 40

6.4.2. Manual Dial Setting ................................................................................... 41

6.4.3. Changing Position ..................................................................................... 42

6.5 OM-Automatic ................................................................................................... 43

6.5.1. Displsy of Default Values .......................................................................... 44

6.5.2. Display of Actual Values ............................................................................ 45

6.5.3 Selecting Stroke Type ................................................................................. 46

6.5.4. Cutting-related Functions .......................................................................... 49

6.5.5. Setting up Stop Positions ........................................................................... 51

6.5.6. Trimming .................................................................................................... 53

6.5.7. Sectioning Window .................................................................................... 54

6.6 Touchscreen Calibration .................................................................................... 55

6.7 Display Cleaning ................................................................................................. 55

6.8 Info ..................................................................................................................... 56

6.9 Troubleshooting ................................................................................................ 57

6.10 Parameters ...................................................................................................... 58


Fig. 5 Basic screen / Start screen

6. Touchscreen, Operating Functions

6.1 Turning on the Device

Turn the main switch to “I ON” and wait until the screen below appears (Fig. 5).The light

below (3.6) turns green, thus signalizing that the device is ready for operation. After

booting/startup, the fault reporting field turns red, the reason being that the SPS/control

starts faster than the converter. After pressing F7 (not on Touchscreen), you come the

fault reporting window (Fig. 6).


Fig. 6 Fehlermeldungen

Acknowledge faults (6.9.) in Fig. 6, using F1, and then press F8 to return to basic screen.

6.2 Touchscreen

Touchscreen may only be used with clean fingers or an input pen for clicking on the

functions and the relevant values/selection options. The rounded-up right angles

indicate functions (OM setting, OM-Automatic, etc.) that may be selected solely using F1

to F8 buttons. Cleaning may only be done using a slight moist towel. Do not use any

solvents or abrasive cleaners.


Fig. 7 Explanation Basic Screen

6.4.

6.5.

6.7.

6.8. 6.9. 6.10. 6.6.

6.3 Basic Screen

Individual functions are selected in the basic screen using F1 – F7 buttons.

6.4. OM (mode of operation)-setting

6.5. OM-Automatic

6.6. Touch calibration

6.7. Screen cleaning

6.8. Info

6.9. Fault reporting

6.10. Parameters


Fig. 8 Tap setting.

Carrier moving Change

tomanualdial

6.4 OM-Setting

Press F1 in basic screen to go to the “Tap setting” window. This is where you can drive both sledges at different speeds. The procedure may be also carried out manually using manual dials.

6.4.1. Tap Setting

In this window, the object carrier or the knife can be moved to the desired value by pressing the arrow buttons, and thereby approximated to the sample. The sledge move until you release the button. The cutting window can be reached manually by entering the stop positions (6.5.5.). Click on the digits, where the values show in the opening windows or teach (save) the actual stop position. Move to the desired position and, using the “Teach-In” button, assume the actual position. The new value will be thereupon taken over to the digital field. It is also possible to move the sledge by means of the manual dial. To do this, select/press the toothed dial on the right (6.4.2.) to go to the “Manual dial setting” window.

Define stop positions


Fig. 9 Manual Dial Installation

6.4.2. Manual Dial Setting

The manual dial is active only in this window for moving the sledges, selecting the desired value that the sledges should reach pro tact, and turning the manual dial. This is where the stop positions may be defined, too, as described in 6.4.1. Press the arrow to return to “Tap setting”.


Fig. 10 Change Position

6.4.3. Changing Position

This function may only be used in OM-Setting and is used for releasing the sample or the

knife for the purposes of changing. By activating the “Change position” button, move the

knife carrier to its most upper end position, then move the object carrier to the most

frontal position. The activation of this function will turn the button yellow and indicates

the moving procedure. Upon reaching the change position, the button turns green.


6.5.1

.

6.5.2

6.5.3

.

6.5.4

6.5.5

Fig. 11 OM-Automatic

6.5 OM-Automatic

The sectioning process is carried out in OM-Automatic (automatic operation). During this

process, the system ignores all the previous OM settings and follows those set during the

sectioning process. The sectioning process may be started either by means of

Touchscreen or with the foot switch.

6.5.1. Display of default values 6.5.2. Display of actual values 6.5.3. Selection of stroke type 6.5.4. Sectioning process functions 6.5.5. Selection of stop positions


Fig. 12 Defining default values

6.5.1. Display of Default Values

This is where default values are defined, using a finger or an input pen in the white field.

After clicking, window 12. opens. Enter the desired numbers and press Enter to confirm.

This is how you may define all default values, except the “Cutting Speed”, which is to be

set by turning the potentiometer. The setting values are limited by minimum and

maximum values.


Fig. 13 Defining Stop Positions

6.5.2. Display of Actual Values

This is where the actual values of the sledges as well as the cuts are shows. These values

cannot be altered, except for the actual value of the cut counter. The cut counter will

reset after reaching the set number of sections by pressing the blue cut counter reset

button. In Fig. 12, the actual value may be reset to “0”and thereupon confirmed.

The stop positions may be defined OM-Setting and OM-Automatic either by clicking on

the white Actual position button and subsequent entering of the desired values (Fig. 13),

or by means of „Teach-In“. To do that, move to the desired position and click on the

“Teach-in” button to define the stop positions. The newly set value will be assumed and

saved in the field. The retraction speed may be set in OM-Automatic as described in

Chapter 6.5.1.

Important: These functions may only be used for setting, not for cutting samples.

6.5.3 Selecting the Stroke Type


Fig. 14 Touch stroke

Explanation of stroke types:

- Touch stroke: Touch stroke must be selected and is active when the field is green.

When the object carrier is to be moved, press and hold START. The object carrier will

keep moving until the button is no longer pressed. The same can be achieved by

means of the foot switch. Just switch the button “Without foot switch” to “With foot

switch”. When the button turns green, the foot switch is active and remains active

until the foot switch is pressed again. Whenever switching the given button, the

object carrier stops in its actual position.


Fig. 15 Single Stroke

- Single stroke: The single-stroke function must be selected and is active when the field

turns green. The function may be started either by using Touchscreen or the foot

switch, whereupon the sectioning process is performed once and then the sledge

remains in the preselected stop position.

-

-

-

-

-

-

-

-


Fig. 16 Continuous stroke

- Continuous stroke: The continuous stroke function must be selected and is active

when the field turns green. Upon starting this mode of operation, either by using

Touchscreen or the foot switch, the previously set number of cuts will be carried out

first and the system remains in the preselected stop position. Set the cut counter at

“0” to move the sledges to the end position (maximum) of sectioning.


Fig. 17 Stop Cycle

6.5.4. Cutting Functions

With these buttons, all the functions related to cutting can be activated.

- START: Press Start to select the stroke type.

- Stop cycle: By activating the Stop cycle button, the currently active cutting process

will move onto the next stop position and Stops. The activation of this function will be

indicated by turning yellow and will turn back to green when the process is finished.

-

-


Fig. 18 Stop Process

- Stopping the process: Upon activating the Stop Process function during the cutting

process, the sledge will come to a halt immediately and remain in the actual position,

interrupting thereby the cutting process. The Stop Process button turns red to indicate

activation. To complete the cutting process or start a new one, press START. The sledge

will move from its actual position to the next one or to the end.

-

- Foot Switch: This is where the foot switch can be activated/deactivated. The built-in

Emergency Stop function cannot be affected in any way – it is always active.


Fig. 19 Predefining Stop Positions

6.5.5. Preselecting Stop Positions

With this function, you can select the stop positions of the object sledge. It is used for setting the position of the end of the cutting process after reaching a certain number of cuts. Click on the selected field and select the stop position, e.g., by selecting "Rear", the sledge will stop in the most rear position.


Fig. 20 Stop Cycle

Stop Positions during Cutting Process

- Touch Stroke: In the Touch Stroke function, the set-up stop positions will not be

considered, as the object carrier will stop in its actual position after you press START.

- Single Stroke: In the Single Stroke function, the sledge will perform one cut, then move

to the selected stop position and remain there. If the ”Front” stop position has been

selected, the sledge will stop in the front position after the cut and would have to be

restarted by pressing START to perform another cut. If “Front + Rear” is selected, the

sledge will stop at the next stop position, i.e. the sledge will stop at the front and rear

each time and has to be restarted by pressing START to perform another cut.

- Continuous Stroke: In the continuous Stroke mode, the object sledge stops upon passing

the selected stop position after reaching the predefined number of cuts. Additionally, in

the Continuous Stroke mode, it is possible to use the “Stop Cycle” button during the

cutting process. The button will turn yellow to signalize that this function is active. The

sledge stops upon passing the selected stop position, whereupon the button turns green.


Fig. 21 Trimming

6.5.6. Trimming

Trimming functions appear on display, as soon as the stroke type is changed to Continuous Stroke. They are divided into 10, 20, and 30µm. Trimming is used for quick start of approximating or cutting of samples. Start the function by pressing “Start” in Continuous Stroke and hold the desired trimming thickness until the desired approximation is reached. In the trimming function, the object sledge performs the cutting and retracting motion at a preset speed. Trimming functions are performed independently of the potentiometer’s position.


Fig. 22 Cutting Way

6.5.7. Sectioning Way

This function will enable you to see how far the sample has been cut. To do this, press the Reset button at the beginning of the cutting process, resetting the counter thereby. The counter will continue to count until it is reset again. It makes no difference, whether the knife or the cut thickness have been changed manually.


6.6 Touchscreen Calibration

In order to ensure a problem-free functioning of the Touchscreen, it should be

regularly calibrated. This is done by opening the “Calibrate Touch” window and

following the commands on the Touchscreen. Click on free Touch first and then select

the relevant crosses until the process is completed.

6.7 Screen Cleaning

Select Screen Cleaning to clean Touchscreen. The cleaning window opens, with a

bar in it to indicate the remaining cleaning time. When the time is up, the screen

will return to the basic screen. This procedure may be repeated liberally, until

Touch is sufficiently clean.


Fig. 23 Info Display

6.8 Info

Whenever you need spare part or accessories or a service technician, contact the

Mikrotom-XL Customer Service or the manufacturer, or your Mikrotom-XL dealer. Find

the required data and information in the “Info” window or on the type label:

- Contact for the manufacturer - Year of manufacture of Mikrotom-XL - Machine number - Model name

Please have these data on hand whenever you have a question or problem.

6.9 Fault Reporting / Troubleshooting


Fig. 24 Fault Reporting / Troubleshooting

This is where occurring problems are to be reported. If an active fault is present, the fault

reporting field turns red. To see this, click on F7. If unable to rectify and release the fault

ourselves (see Troubleshooting), please contact your Mikrotom-XL dealer or Mikrotom-XL

Customer Service or the manufacturer.


Fig. 25 Parameters

6.10 Parameters

The “Parameters” window may only be opened by authorized personnel or service

technicians. It is used for setting up important basic parameters that ensure proper

functioning of the device. Unauthorized opening and/or readjustment of the parameters

is not permitted. Therefore, this function is protected by the manufacturer’s password.


Chapter7

Operation

7. Operation. ...................................................................................................... 60

7.1 Sample fixing ...................................................................................................... 60

7.2 Knifesetting or changing .................................................................................... 60

7.2.1 Polycut knife holder Type 1 + 2 ................................................................. 61

7.2.2 Universal knife holder ................................................................................. 63

7.3 Default values defining /Setting ........................................................................ 64

7.4 Knife angle setting ............................................................................................. 65

7.4.1 Polycut knife holder Type 1 + 2 .................................................................. 65


7.5 Declination angle setting ................................................................................... 67

7.5.1 Polycut knife holder (Typ 1 + 2) .................................................................. 67


7.6 Section thickness and cut number setting......................................................... 68

7.7 Sectioning window ............................................................................................. 68

7.8 Sectioning .......................................................................................................... 69

7.9 Select sectioning speed ..................................................................................... 71

7.10. Taking off sections .......................................................................................... 72


7. Operation

7.1 Sample Fixing

Move the device in the change position (6.4.3) and make sure, due to a potential risk of

injury, that no knife is fitted in the device or the knife guard closed. Fix the sample using a

suitable clamp and set it on a magnet or the object carrier.

7.2 Knife Fitting or Knife Type Changing

Currently, there are 3 types of knife holders available (see Accessories): - Polycut knife holder, Type 1, turning point around the cutting edge - Polycut knife holder, Type2, turning point around the axis of the guiding slots

Polycut knife holder, Type 1 +2, with changeable fixing plate, for fixing different types of knives

- Universal knife fixture Universal knife holder with changeable adapter bars for fixing different types of knives with one and the same knife holder.


Finger guard

Screws Clampslider

hieber

Fig. 26 Polycut Knife: change of knives

7.2.1 Polycut knife Typ 1 + 2

!!!Risk of Cutting –Wear Gloves!!!

Open the finger guard by pressing both clamp slides inwards

and flip the finger guard backwards, as shown in the illustration.

Thereupon unscrew the screws (5 pcs) and slide the knife in

sideways. Thereupon screw the screws in again to fix the knife.

Close the finger guard by flipping in forward until it is visibly in

its place and makes a clicking sound.

To fit it another type of knife, unscrew the screws and take

them out along with the fixing plate. Thereupon put in a new

fixing plate and fasten it with the screws again.


Screws Adapters

Fig. 27Changing the knife holder

In order to use another type of knife, loosen the screws (4pcs), as well as both stop pins,

then pull the knife holder towards from the guiding bars and put them on a table. Loosen

both clamp levers and push the adaptors to the left and right to be able to insert a new knife

holder.

Thereupon assembly the knife holder in the device in reversed order.


Protection Screws

Clamplever

Fig. 28 Universal Knife Fixture

7.2.2 Universal Knife holder

!!! Risk of Cutting – Wear Gloves!!!

Open the finger guard and put it on the adaptor of the universal

knife fixture, so that the magnets lift it and prevent

unintentional closing. Thereupon, unscrew the 4 screws, insert

the 2 small relief screws, and insert the knife (tip: insert it

sideways). Subsequently, screw the 4 screws in again and the 2

small ones out again to fixate the knife. Close the finger guard

by flipping in forwards, until it is visibly in place with the

magnets holding it.

If wanting to change the type of knife, unscrew the screws and

take them out along with the fitting bar. Subsequently put in a

new fitting bar and fasten the screws again.


In order to change the knife holder in the knife holder base, loosen the clamp lever and pull it out completely. Caution, the knife fixture could now fall down. Therefore, lift the knife holder to avoid damage. (Tip: Put a thick piece of cloth underneath for protection). Pull the knife holder forwards and insert a new one. Press the knife holder carefully in the toothed wheel and push the shaft back in again from the outside and tighten it.

7.3 Define Default Values /Setting

Go to Default Values Defining in Chapters, 6.4.1. + 6.4.2.and 6.5.1.to 6.5.5.


Clamplever Angle adjustment

Fig. 29 Polycut Knife holder: Knife Angle Setting

7.4 Knife Angle Setting

Depending on the facet of the knife, angle setting of approx. 10-15° is recommended.

When using a sharpened knife, the height of the knife must be adjusted accordingly.

7.4.1 Polycut Knife holder, Type 1 + 2

In order to set the knife angle, loosen both clamp levers and turn the angle setting to the

desired angle. Thereupon close both clamp levers again.


Clamplever

Knife angle adjustment

Fig. 30 Universal Knife holder: Knife Angle Adjustment


In order to adjust the knife angle, loosen the clamp lever and using a hex wrench set the required angle. Thereupon, close the clamp lever again.


Screws 4

on each

side

Clamplever

1 on

eachside

Fig. 31Polycut Knife holder: Declination Angle Setting

7.5 Declination Angle Setting

The declination angle is set to obtain an evenly drawn cut. Loosen the screws and clamp

levers on both sides and shift the whole knife holder on the guiding bars. Thereupon,

tighten the screws and clamp levers again.

7.5.1 Polycut Knife holder (Type 1 + 2)


Screws 2 on each

side

Fig. 32 Polycut Knife Fixture: Declination Angle Setting


7.6 Section Thickness and Section Number Setting

Go to Defining the Number of Cuts and proceed as described in Chapters 6.5.1

and 6.5.2.

7.7 Sectioning Window

Go to Defining Cutting Window and proceed as described in Chapter

6.5.1.


7.8 Sectioning

Make sure that all the required actual values, such as number of cuts, cut thickness, etc.

are correctly defined and that you are in OM-Automatic. Additionally, you should observe

the following points:

- Always use a sharp knife

- When the knife is worn (dull) in one position, shift the knife so that its sharp edge can be

used for sectioning.

- Keep the front and rear side of the knife always clean

It is generally recommended to wear protective glasses when

sectioning samples of any kind to prevent an injury from splinters.

Starting the different cutting stroke types:

See also Chapter 6.5.3

Interval stroke:

Starting via Touchscreen:

- Select the interval stroke, press and hold the START button. The process will continue

in this mode, until the button is no longer pressed (released).

Starting with the foot switch:

- Select the interval stroke and then press the “With foot switch” button. Keep the foot

switch pressed as long as you want the process to continue.

Single stroke:


- Select the “Single stroke” button and then press START. The device will carry out a

single cut and stops in a predefined stop position. If “Stop process” is activated during

the cutting procedure and the device will stop in the current position. Press “START”

again to complete the process.


- Select the “Single stroke” button and then press “With foot switch”. While the foot

switch is being pressed, the cutting process will be completed and the object carrier

remains in the predefined stop position. Press the foot switch again to repeat the

process.


Continuous stroke:


- Select “Continuous stroke” and then press START. The device will carry out either the

preset number of cuts or keep cutting until it reaches the end position. If “Stop

process” is activated during the cutting procedure and the device will stop in the

current position. Press “START” again to complete the process. If the “Stop cycle”

button is pressed, the process will interrupt at the predefined stop position. The

activation of this function is indicated when the button turns yellow. Press “START” to

renew the process. In the continuous-stroke mode, it is possible to set quick

sectioning and approximating t the sample by means of trimming (6.5.6). To do this,

select the desired trim thickness and hold it until the desired position is reached.


- Select “Continuous stroke” and then press the function “With foot switch”. Keep

pressing the foot switch until the desired number of cuts or the end position is

reached. Press the foot switch again to make the object carrier stop at a predefined

stop position.


Fig. 33 Cutting speed

Fig. 34 Potentiometer

7.9 Select Sectioning Speed

Turn the potentiometer to define the cutting speed. The actual sectioning speed value will

be shown in the default field. If no cutting speed is selected, i.e., the potentiometer stands

at Zero, the default field turns red.


7.10. Taking off sections

Sections are to be taken off by using a suitable tool. The tool should be softer than the

knife and possibly not made of metal. Friction might result in burrs that could damage the

knife or the knife holder. Taking off sections with fingers should be avoided.


Chapter8

Fault Reporting

8. Fault Reporting. ...................................................................................................... .74


8. Fault Reporting / Troubleshooting

Fault / Fault Reporting Cause Remedy

The device is not working Main switch is off

Socket contact is loose

Defective fuse

Turn main switch to “I ON”,

Check socket connections,

Change the fuse

Handling inverter fault SPS boots faster than the

inverter

Acknowledge fault as

described in 6.1 + 6.9

Object carrier makes no

cutting movements

Potentiometer in on Zero Turn the potentiometer

Emergency button is active Emergency button has

been pressed at the

operator’s panel or with

the foot switch

1. Turn and pull the Emergency button up 2. Press the blue button at the foot switch to deactivate Emergency stop 3. Acknowledge the fault as described in 6.1 + 6.9

Maximum position reached The object carrier has reached the maximum position, i.e., the end position

Object carriers have to be released manually


Chapter9

Accessories

9. Accessories .................................................................................................... 76

9.1 Accesory to the knife/ knife holder ................................................................... 76

9.2 Accessories to object fitting andpositioning ..................................................... 79

9.3 Table accesories ................................................................................................. 83


9. Accessories

9.1 Accessories to the Knife/Knife holder

Knife holder adaptor (polycut knife)

Art. No. B160076

Taking/ Carrier for polycut knife holder

Declination/angle adjustment 90°-135°

Knife angle/fine adjustment 0 - minus 25°

Knife holder KH-CC/CD

Art. No. B160077

Knife holder of stainless steel

Finger guard mechanically stoppable

For parallel Polycut knives with CC or CD edge

Knife holder KH-P (polycut knife)

Art. No. B160078



For parallel standard Polycut knives

Adaption knife holder

Knife holder KH-CC/CD

Knife holder KH-P


Knife holder KH-R (rotary microtome knife)

Art. No. B160079



For rotary microtome knives

Knife holder KH-S

Art. No. B160080



For standard knives up to 220mm

Knife holder KH-E

Art. No. B160081



For disposable low-profile blades up to 180mm

Knife holder KH-R

Knife holder KH-S

Knife holder KH-E


Special knife holder

Art. No. B160082


For direct fixation of diverse microtome knives

Knife with knife box

Art. No. H160094

Different knives with knife box available

Adaption Universal knife holder

Art. No. F160158

Sledge bar for universal knife holder

Declination/Angle adjustment 90°-135°

Universal knife on sledge bar, freely slidable (thus

possible to be used with any knife, without new cut

starting)

Special knifefixture

Adaption Universal knife holder

Knifebox with knife


Universal knife holder

Art. No. B160083

Universal knife holder for standard microtome knives

„C“ and „D“

Knife holder of hardened stainless steel

Knife angle adjustment -5 to -25° (right to left

adjustable and readable)

Clamp bar changeable for „C“ and „D“ knives

Knife positioning with fine adjustment by means of

two laterally mounted nuts

Safety finger guard

9.2 Object Fixation and Positioning Accessories

Universal fixation frame

Art. No. B160084

Fixation frame, size 90x100mm

Fixation Vice S1 Industrial Version

Art. No. H160095

Manual screwing vice

Back width 64mm

Fixation width 67mm

Universal knife holder

Universal fixationframe

Fixation vice S1


Fixation Vice k S2 Industrial Version

Art. No. H160096

Manual screwing vice

Back width 125mm

Fixation width 156mm

Prism Plate

Art. No. F160159

Taking for all fixation accessories with Prism clamping

(e.g., universal cassette clamp, standard object clamp,

etc.)

Plate options: black or natural

Universal Cassette Clamp

Art. No. B160085

Quick-change system for all current cassettes

Fixation vice S2

Universal cassette clamp

Prism plate


Maxi Cassette Clamp

Art. No. B160084

Quick-change system for all current cassettes

Standard Object Clamp

Art. No. B160087

Object clamping with firm upper clamp backing,

therefore maximum stability

Maximum object size: 50x50mm

Foil Clamp

Art. No. B160088

For fixation of thin foils and samples

Maxi-Cassette-Clamp

Standard object clamp

Foil clamp


Application for round samples

Art. No. B160089 (Ø6)

Art. No. B160090 (Ø15)

Art. No. B160091 (Ø19)

Art. No. B160092 (Ø25)

For problem-free fixation of round samples with

defined diameter in connection with standard object

clamp

Round sample sizes: Ø6 / Ø15 / Ø19 / Ø25mm

Optionally also special dimensions

Mini Flat/ Prism Fixture

Art. No. B160093

Fixture for small, flat and round samples

Object orientation / positioning

Art. No. B160094

Objectorientation with mechanical quick-clamping

Angle range ±5°

Turning range 360°

Round samples

Mini flat/ prismfixture

Object orientation / positioning


Adapter plate

Art. No. F160160

For object orientation on a magnet plate (Will be

needed for fastening the fixation frame, screwing

vice, i.a. directly on the magnet plate)

9.3 Table accessory

Paraffin Table T1

Art. No.F160161

Paraffin table, size 96x96mm

Paraffin Table T2

Art. No. F160162 Paraffin table, size 146x146mm

Paraffin Table T3

Art. No. F160163

Paraffin table, size 196x196mm

Adapter plate

Paraffin table 146x146


Chapter10

Basic Instructions

10. Disposal of waste .......................................................................................... 85


10. Disposal of waste

The device and accessories must be disposed of in strict compliance with the relevant

regulations.


Chapter11

Emergency Service, Customer Service

11. Emergency Service, Customer Service ........................................................... 87

11.1 Address ........................................................................................................... 87

11.2 Instruction for Emergency and Customer Serivices ........................................ 87


11. Emergency Service, Customer Service

11.1 Address


Rudolf-Diesel-Str. 47 D-69190 Walldorf/Baden Tel: + 49 (0) 6227 / 8222-0 Fax: + 49 (0) 6227 / 8222-30 [email protected] www.micro-tec.de

11.2 Instruction for Emergency and Customer Services

In the event that you need spare parts or additional accessories or the Technical Customer

Service, please contact Mikrotom-XL Customer Service or your Mikrotom-XL dealer.

Please have the following information ready:

- Date of delivery

- Reason for requiring Customer Service

- Brief problem description

- Model name and serial number

- Location and contact person



Chapter12

List of Figures (Illustrations)

12. List of Figures/Illustrations ………………………………… ........................... …………… 89


12. List of Figures/Illustrations

Fig. 1 Accessories ............................................................................................................................................................ 33

Fig. 2 Mikrotom-XL .......................................................................................................................................................... 34

Fig. 3 Foot switch ............................................................................................................................................................ 35

Fig. 4 Potentiometer & manual dials ............................................................................................................................. 35

Fig. 5 Basic screen / Start screen .................................................................................................................................... 37

Fig. 6 Error Messages ...................................................................................................................................................... 38

Fig. 7 Exlanation re Basic Screen ..................................................................................................................................... 39

Fig. 8 Tap setting. ............................................................................................................................................................ 40

Fig. 9 Manual Dial Installation ......................................................................................................................................... 41

Fig. 10 Change Position ................................................................................................................................................... 42

Fig. 11 OM-Automatic ..................................................................................................................................................... 43

Fig. 12 Defining default values ........................................................................................................................................ 44

Fig. 13 Defining Stop Positions ........................................................................................................................................ 45

Fig. 14 Touch stroke ........................................................................................................................................................ 46

Fig. 15 Single Stroke ........................................................................................................................................................ 47

Fig. 16 Continuous stroke ............................................................................................................................................... 48

Fig. 17 Halt Cycle ............................................................................................................................................................. 49

Fig. 18 Stop Process ........................................................................................................................................................ 50

Fig. 19 Predefining Stop Positions ................................................................................................................................... 51

Fig. 20 Halt Cycle ............................................................................................................................................................. 52

Fig. 21 Trimming.............................................................................................................................................................. 53

Fig. 22 Sectioning Window .............................................................................................................................................. 54

Fig. 23 Info Display .......................................................................................................................................................... 56

Fig. 24 Fault Reporting / Troubleshooting ...................................................................................................................... 57

Fig. 25 Parameters .......................................................................................................................................................... 58

Fig. 26 Polycut Knife: change of knives ........................................................................................................................... 61

Fig. 27 Changing the Knife holder ................................................................................................................................... 62

Fig. 28 Universal Knife holder ......................................................................................................................................... 63

Fig. 29 Polycut Knife holder: Knife Angle Setting ........................................................................................................... 65

Fig. 30 Universal Knife holder: Knife Angle Adjustment ................................................................................................. 66

Fig. 31 Polycut Knife holder: Declination Angle Setting .................................................................................................. 67

Fig. 32 Polycut Knife holder: Declination Angle Setting .................................................................................................. 68

Fig. 33 Cutting speed ....................................................................................................................................................... 71

Fig. 34 Potentiometer ..................................................................................................................................................... 71


Chapter13

Notes

13 Notes ............................................................................................................. 91


13. Notes