laser-assisted thermal-expansion microinjector
DESCRIPTION
Laser-assisted Thermal-expansion Microinjector. LTM-1000. Conventional Microinjector. Inject by oil pressure or gas pressure If the capillary with less than 0.5 μm tip is used, the capillary itself will be moved forward due to the pressure. With complicated flow path. - PowerPoint PPT PresentationTRANSCRIPT
Unable to use fine tips under 0.5 μm for injection
Unable to inject into the cell having rigid cell wall such as plant cells
Complicated flow path brings on a time-consuming preparation
Conventional Microinjector
Inject by oil pressure or gas pressure If the capillary with less than 0.5 μm tip is used, the capillary itself will be moved forward due to the pressure. With complicated flow path
LTM-1000 : Structure
Feature
Extremely-high pressure (approx. 1MPa per 1Extremely-high pressure (approx. 1MPa per 1℃℃) )
makes it possible to use capillaries with the tip makes it possible to use capillaries with the tip
around 0.1-0.5μm without clogging.around 0.1-0.5μm without clogging. Simple flow path allows to start experiments Simple flow path allows to start experiments
right away saving a lot of labor.right away saving a lot of labor.
Heart-induced expansion of liquid in a sealed capillary generates high pressure.
LTM-1000
Applications
Injection of antibody into plant cells
Injection into chloroplasts of Cyanidioschyzon merolae
Chloroplast
Alexa 488
Lucifer Yellow
20µm
5µm
Alexa 546-dextran 10KAlexa 546-dextran 10K
50µm
50µm
ConventionalMethod LTM-1000
*Whereas the damage and sample escape appear by the conventional method, LTM-1000 ensures no damage and continuous injections without sample escape by using a fine tip capillary.
Injection into the female gametophyte of plant