layout of the rh385 lab · web viewph meter _____ clamps _____ syringes _____ office-related: key...
TRANSCRIPT
General Orientation Training – Nizkorodov LabLast updated on May 8, 2023Topics to be covered during this orientation includeLayout of the RH385 lab........................................................................................................................................................................................................................ 2
Locations of various items in the labs.................................................................................................................................................................................................... 5
Locations of important places............................................................................................................................................................................................................. 10
Location of important documents and software.................................................................................................................................................................................10
Buying chemicals and/or equipment................................................................................................................................................................................................... 11
Updating Chemical Inventory (New Chemicals and Empty Containers)..............................................................................................................................................11
Solvent storage and safety.................................................................................................................................................................................................................. 11
Dry chemical storage and safety.......................................................................................................................................................................................................... 12
Cleaning and drying glassware (suggested procedures)......................................................................................................................................................................12
Correct usage of the hoods................................................................................................................................................................................................................. 12
Keeping personal laboratory records.................................................................................................................................................................................................. 13
Instrument/Equipment signup............................................................................................................................................................................................................ 13
Dealing with unplanned power outages.............................................................................................................................................................................................. 13
Dealing with planned power outages.................................................................................................................................................................................................. 14
Dealing with celling drips and/or floods.............................................................................................................................................................................................. 15
IMPORTANT!!!! Group Member Safety and Other Responsibilities:...................................................................................................................................................15
Safety Officer Responsibilities:............................................................................................................................................................................................................ 16
Disposing of hazardous waste............................................................................................................................................................................................................. 16
Important websites............................................................................................................................................................................................................................. 17
Important seminars............................................................................................................................................................................................................................. 17
Hands-on training to be provided........................................................................................................................................................................................................ 17
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Layout of the RH385 lab
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The previous page provides an image of the RH385 lab. During orientation you must familiarize yourself with the purpose of every bench, hood, storage cabinet, experimental area, etc. Guide: go over locations of these items (point out features of hoods like vacuum lines, etc.). Orientee: write down the purpose of the major locations in RH385:
o Fume hoods:
Hood 1 contains ______________________________________________________________________________________
Hood 2 contains _____________________________________________________________________________________
o Toxic gas bottle cabinets
Toxic bottle cabinet 1 contains __________________________________________________________________________
Toxic bottle cabinet 2 contains __________________________________________________________________________
o Benches
Purposes of benches vary based on projects. Make notes about the purpose of every bench:
Bench 1 contains _____________________________________________________________________________________
Bench 2 contains _____________________________________________________________________________________
Bench 3 contains _____________________________________________________________________________________
Bench 4 contains _____________________________________________________________________________________
Bench 5 contains _____________________________________________________________________________________
Bench 6 contains _____________________________________________________________________________________
Bench 7 contains _____________________________________________________________________________________
Bench 8 contains _____________________________________________________________________________________
o Cabinets
Cabinet 1 contains_____________________________________________________________________________________
Cabinet 2 contains ____________________________________________________________________________________
Cabinet 3 contains ____________________________________________________________________________________
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Orientee: write down the purpose of the locations we have access to:
o RH350 (Annmarie Carlton lab – we have limited access)
RH350 hoods contain _________________________________________________________________________________
RH350 benches contains _______________________________________________________________________________
RH350 cabinets contain________________________________________________________________________________
Refrigerator contains__________________________________________________________________________________
Freezer contains_____________________________________________________________________________________
o Third floor service chase
What can be found in this chase?________________________________________________________________________
o Fourth floor service chase
What can be found in this chase?________________________________________________________________________
Where are the valves to close water from the circulator?______________________________________________________
o Storage room – list items that can be found in this room
__________________________________________________________________________________________________
__________________________________________________________________________________________________
__________________________________________________________________________________________________
__________________________________________________________________________________________________
__________________________________________________________________________________________________
__________________________________________________________________________________________________
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Locations of various items in the labs
Guide: review where these items are keptOrientee: write down their locations (e.g., “under bench 1”)
Safety-related:
o Eyewash and shower _____________________________________________________________________________________
o Spill kit _______________________________________________________________________________________________
o Gloves ________________________________________________________________________________________________
o Tissues ________________________________________________________________________________________________
o Cleaning supplies ________________________________________________________________________________________
o Fire extinguishers ________________________________________________________________________________________
o Safety glasses ___________________________________________________________________________________________
o Broken glass containers ___________________________________________________________________________________
o Sharps container _________________________________________________________________________________________
Tools:
o Mechanical tools (wrenches, hammers, etc.) _____________________________________________________________________
o Bolts and nuts ____________________________________________________________________________________________
o Unistrut hardware __________________________________________________________________________________________
o Propane torch for soldering __________________________________________________________________________________
o Epoxy and lubricants ________________________________________________________________________________________
Electrical:
o Multimeters ______________________________________________________________________________________________5
o Thermocouples and thermometers _____________________________________________________________________________
o Wires and hot shrink tubing __________________________________________________________________________________
o Parts for making cables and various cables _______________________________________________________________________
o Electrical plugs ____________________________________________________________________________________________
o Soldering supplies _________________________________________________________________________________________
o Heat guns ________________________________________________________________________________________________
o BNC cables ______________________________________________________________________________________________
o Extension cords ____________________________________________________________________________________________
o New batteries and used batteries container _______________________________________________________________________
Chemicals and synthesis:
o Chemicals – solvents _____________________________________________________________________________________
o Chemicals – solids _______________________________________________________________________________________
o Chemicals – acids ________________________________________________________________________________________
o Chemical waste __________________________________________________________________________________________
o Chemicals needing refrigeration _____________________________________________________________________________
o Synthesis supplies ________________________________________________________________________________________
o Glassware – flasks, volumetric, beakers and Erlenmyers ___________________________________________________________
o Glassware – large flasks _____________________________________________________________________________________
o Vials and bottles ___________________________________________________________________________________________
o Pipettes (manual and automatic) ______________________________________________________________________________
o Ovens ____________________________________________________________________________________________________
o Desiccators and dry boxes _________________________________________________________________________________
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o Stirplates and hotplates ____________________________________________________________________________________
o Rotavap and its supplies ____________________________________________________________________________________
o Nanopure water sources_____________________________________________________________________________________
o Balances for chemicals only __________________________________________________________________________________
o pH meter _________________________________________________________________________________________________
o Shaker __________________________________________________________________________________________________
o Dewars __________________________________________________________________________________________________
o Clamps __________________________________________________________________________________________________
o Syringes _________________________________________________________________________________________________
Office-related:
o Key for entering the lab if you locked yourself out (ask Sergey for the code)
o Fridge for food __________________________________________________________________________________________
o Office supplies _________________________________________________________________________________________
o Printers ________________________________________________________________________________________________
o Label printer _____________________________________________________________________________________________
o Computer supplies ______________________________________________________________________________________
o Paper and toners _________________________________________________________________________________________
Vacuum and flow:
o Tubing – metal ___________________________________________________________________________________________
o Tubing – conducting ______________________________________________________________________________________
o Tubing – Impolene and Teflon (for ozone only) __________________________________________________________________
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o Tubing – short bits _________________________________________________________________________________________
o O-rings ___________________________________________________________________________________________________
o Vacuum grease ______________________________________________________________________________________________
o Valves ___________________________________________________________________________________________________
o Swagelok and other vacuum fittings __________________________________________________________________________
o Mass flow controllers and rotameters__________________________________________________________________________
o Pressure meters ___________________________________________________________________________________________
o Purge air generators ________________________________________________________________________________________
o KF flanges (for connecting pumps) ____________________________________________________________________________
o Particle air filters __________________________________________________________________________________________
o Pump oil __________________________________________________________________________________________________
o Pump spare parts ___________________________________________________________________________________________
o Vacuum glass rack (for pumping on small volumes of liquid) ________________________________________________________
o Gas regulators ______________________________________________________________________________________________
o Gillibrator flow calibrator _____________________________________________________________________________________
o Defender flow calibrator _____________________________________________________________________________________
Aerosol synthesis and collection:
o Filters and filter holders for aerosol collection______________________________________________________________________
o MOUDI parts and substrates _________________________________________________________________________
o Sealer and Teflon sealing supplies ___________________________________________________________________________
o Teflon roll (usually in the BFP group) _________________________________________________________________________
o Balances for aerosol filters only ______________________________________________________________________________
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o Aerosol chamber supplies ___________________________________________________________________________________
o Aerosol flow tube _________________________________________________________________________________________
o MAGIC flow tube __________________________________________________________________________________________
o Ozone generators _________________________________________________________________________________________
o NOy box accessories_______________________________________________________________________________________
o Ozone monitors ___________________________________________________________________________________________
o SMPS ___________________________________________________________________________________________________
o Atomizers ________________________________________________________________________________________________
o Slit impactors ______________________________________________________________________________________________
o Pumps for aerosol sampling __________________________________________________________________________________
o Spare TSI parts ___________________________________________________________________________________________
Electronics and instruments:
o HPLC instrument __________________________________________________________________________________________
o HPLC instrument spare pumps and detectors______________________________________________________________________
o GCMS instrument __________________________________________________________________________________________
o UVVIS instruments _________________________________________________________________________________________
o Ocean optics UVVIS parts_____________________________________________________________________________________
o QCM instrument ___________________________________________________________________________________________
o Various electronic instruments _______________________________________________________________________________
o Power supplies ____________________________________________________________________________________________
Radiation sources:
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o Replacement chamber lamps ____________________________________________________________________________________
o Replacement arc and pen ray lamps _______________________________________________________________________________
o Laser supplies________________________________________________________________________________________________
o Laser goggles ________________________________________________________________________________________________
o Optics and optical mounts ______________________________________________________________________________________
o LEDs _______________________________________________________________________________________________________
o Arc lamp supplies _____________________________________________________________________________________________
o Power meters ________________________________________________________________________________________________
***Items specific to experiments are found in labeled drawers
Locations of important places
Guide: review where these places are located
Orientee: write down their locations
o Location of electrical breakers in every lab_____________________________________________________________________________
o PS Stores (make sure orientee has an account) _________________________________________________________________________
o Machine shop (explain how to place orders) ___________________________________________________________________________
o Glassblowing shop (explain how to place orders) _______________________________________________________________________
o Purchasing office (explain how to place orders) _________________________________________________________________________
o Mass spectrometry facility (explain how to use) _________________________________________________________________________
o Laser facility (explain how to use) OUR GROUP CODE IS: 199739_________________________________________________________
o NMR facility (explain how to use)____________________________________________________________________________________
o Meeting place for emergencies _______________________________________________________________________________________
o Imageworks (for printing posters) _____________________________________________________________________________________
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Location of important documents and software
Guide: go over where these documents are kept
Orientee: write down their locations
o MSDS _________________________________________________________________________________________________
o Lab’s standard operating procedures and instructions _____________________________________________________________
o Shared group files (like IP addresses, birthdays, etc.) _____________________________________________________________
o Purchase orders (POs – online versions and older paper versions )__________________________________________________
o Instrument manuals _______________________________________________________________________________________
o Company brochures _______________________________________________________________________________________
o Group software ___________________________________________________________________________________________
Buying chemicals and/or equipment
o Know your short and long budget code (each student has a separate one)o Where to find and how to fill out a PO (purchase order)o Where to buy things
UCIBuy: https://portal.uci.edu/uPortal/f/main/normal/render.uP Requisitions Gases – Airgas, through UCIBuy Optics – Newport Optics Chemicals – Fisher (use username = uci; password = gofisher to show UCI prices; or Sigma Aldrich) Synthesis supplies (like TLC plates or glassware) – VWR (cheaper than Fisher) General supplies – McMaster Carr Swagelok – Orange Fluid Systems ***if you can’t find what you’re looking for with these companies, search the internet
o What to buy - review: very hazardous chemicals (check MSDS, is there another option?), chemical grades, chemical stability, etc. o Receiving chemicals, transferring them to the inventory, an labeling them - SEE BELOW
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**buy only what you need (e.g. if you need 5 mL do not buy 500 mL!!! This is especially true if the chemical decomposes with time)
Updating Chemical Inventory (New Chemicals and Empty Containers)
o Refer to the document in the group dropbox titled “lab_chemical_inventory_instructions.docx”
Solvent storage and safety
o Always wear gloves/goggles/labcoat when dealing with potentially-hazardous chemicals. o Solvents are kept in ventilated cabinets below hoods 1-2, and benches 1-3. There are separate cabinets for storage of flammables,
flammable/general, toxics, corrosives, and oxidizers. o Several squirt bottles are available for solvents. o Use organic solvents IN THE HOOD. Water can use used outside the hood.
Dry chemical storage and safety
Always wear gloves/goggles/labcoat when dealing with potentially-hazardous chemicals.
o General dry chemicals are stored in the cabinet in RH350. Some dry chemicals that need to be kept cool are stored in the RH 350 fridge. o Dry chemicals that are hazardous (and/or smelly) must be weighed out IN THE HOOD (unplug and turn off the balance, relocate the
balance to the hood, plug in/turn on and weigh) Reminder: Although silica gel looks harmless, it should always be weighed out in the hood because the particles can get into your lungs and
damage the tissue.
Cleaning and drying glassware (suggested procedures)
o Glassware that contained pure organic compounds– wash with suitable organic solvent a few times and air dry or oven dry. o Glassware that is dirty –
General procedure: Soak in a bath of Liquinox solution for at least 15 minutes. Scrub well with glassware brush. Rinse well with tap water. Rinse again with DI water. Rough rinse with acetone (reuse the acetone for subsequent pieces). Final rinse with acetone and oven dry.
o Glassware that needs to be critically cleaned – Acid procedure 1– coat glassware with concentrated sulfuric acid and let sit for at least 30 min. Rinse carefully and thoroughly
with tap water and keep rinse water in a large waste container. Neutralize both liquid in the glassware and waste container with sodium bicarbonate until pH 7. Discard neutralized water down the drain. Rinse clean glassware with DI water and dry.
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Acid procedure 2 – make up a strongly acidic solution and soak glassware for 1 hour. Remove glassware and save solution in a suitable container. Place glassware in a large bucket of water. Neutralize the water in the bucket with the sodium bicarbonate. Rinse glassware with DI water and dry.
Permanganate – make a concentrated potassium permanganate solution and place in a large (1 L or larger) beaker until 70% full. Boil your glassware in the permanganate solution for 1 hour. Remove glassware and place in a dilute H2O2 bath, save the permanganate solution for future use. Rinse glassware with DI water and dry.
Correct usage of the hoods
o Hood lights should be ON whenever in useo Hood ventilation should be workingo Don’t block the hood sash from closing all the way (feed lines underneath)o Don’t use the hoods as a storage place for old chemical solutions. If vials/containers have been there for months without use, get rid of it
or store in a proper place (the fridge for example). o At the end of the day, TURN OFF the hood lights and CLOSE the hood sash
Keeping personal laboratory records
Guide: Make sure orientee has a laboratory notebook! Sergey has new notebooks stored in his office. Show orientee an example of laboratory notes. Keeping laboratory records is mandatory! Your memory cannot be trusted, so record your experiment in detail every time.Things to record:
o Date of experimento Instruments used and their settings o Drawing/sketch/photo of setup the first time you used it o Chemicals (where you obtained them, purity, structure and formula, MW, etc.)o Weights/volumes/etc of all materials used – e.g., glassware or vials, chemicals, etc. o Initial conditions before experiments o ALL observations made at ANY time during experimento Final conditions after experiments
Instrument/Equipment signup
These instruments may require sign-up:
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o PTR-ToF-MS (online Google Doc)o ToF-AMS (online Google Doc)o Chamber (online Google Doc) – not right now but will have to implement in the future
Dealing with unplanned power outages
If an unexpected power outage happens, QUICKLY:
o Grab an emergency flashlighto Shut-off valves for toxic gases if they were in use just before the power went downo Make steps to stop the on-going experimento Shut down your computer (if it is powered by an UPS) to prevent loss of datao Quickly shut shown all the instruments connected to UPS if they are running:
PTR-MS computer and spectrometer CIMS computer, mass-spectrometer, turbo pumps, diaphragm pumps, MFC controllers UV/Vis instruments and their computers GCMS instrument and its computer HPLC instrument and its computer SMPS instruments and their computers Both CRDS computers
o For all expensive instruments with on/off switches set the switches to the off position on their power strip (or unplug them if they are directly plugged in) – otherwise they may die when the power comes back
CRDS oscilloscope, delay generator, MFC controller, baratron controller Xe-lamp power supplies Chamber gas monitors Chamber MOUDI Chamber filter pump TDMA high-voltage power supplies
o Lower the hood sasheso Stop the chamber purge (if it is on standby)
Dealing with planned power outages
Turn off equipment in the following order:14
1. CIMS turbo pumps (water cooled) and Extel electronics2. Booster pump (water cooled) and mechanical pump in the 3rd floor chase3. Large Nd:YAG laser4. Water circulator in the 4th floor chase (CIMS turbos must finish spinning down since they are being cooled by this water)5. Close all gas flows and all gas bottles; close the inlet air for all zero-air generators6. Turn off all mechanical pumps7. Turn off all stand-alone equipment (see list above)8. Disable all UPS (so that they do not switch to emergency power) and turn them off or unplug them9. For all instruments with on/off switches set the switches to the off position (or unplug them)10. Unplug all expensive equipment from wall outlets11. If the power outage is expected to be longer than 1 hour move all chemicals from the refrigerator to dry-ice cooled boxes and defrost the
refrigerators during the power outage
Dealing with celling drips and/or floodso If the ceiling begins to drip or the lab floods, it is important to prevent water damage to equipment (especially ones that are difficult to move).o Power down the corresponding equipment, but make sure it is safe to do so (i.e. you won’t electrocute yourself by unplugging it because
water is near the electrical input of the equipment).o Place a piece of polyethylene (it is in sheet form rolled up in a box above the large toolbox in RH385) over the equipment that is affected.
IMPORTANT!!!! Group Member Safety and Other Responsibilities:
o LEAVING FOR THE DAY : At the end of the day, if you are the last one in the lab or in your office: Make sure all the lab and office doors are locked before you leave. Turn off the lights in your office. Makes sure the hood sashes are down and their lights are off. Either turn off your computer or put it into standby/sleep mode. Turn off your computer monitors.
o WASTE DISPOSAL : Everyone in the group is responsible for the waste they generate. Make sure to label all waste containers appropriately. For more information on how to store and label waste, please refer to the “Disposing of waste” section of this orientation document. Request a pickup from EH&S within 6 months of the start date of the waste generation (waste pickup requests will be submitted by different group members on a rotating basis to spread the load equally).
o BUYING CHEMICALS : The person who purchased the chemical is responsible for entering information about this in the lab and PS databases. Please see instructions in “lab_chemical_inventory_instructions.docx
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o PACKING SLIPS : Save all packing slips on all packages containing chemical, equipment, etc. delivered to our lab and ordered through the Purchasing Office. Place packing slips in the clear file holder located in the wall next to the sink in the front of the lab.
o TOOLS : When you use tools/scissors/etc. from the lab, please put them back in their correct location when you are done with themo USING AN INSTRUMENT/CHEMICAL FOR THE FIRST TIME : If you are using a chemical/instrument for the first time, make sure
you have read all appropriate documentation, have been trained in its operation, and have signed all appropriate documentation in the safety binders
o LAB HEALTH : Keep an eye on the following: HOOD SASH: If the hood sash was accidentally left raised too high lower it. HOOD FAILURE: if the hood is beeping, reset the hood, lower the sash, and verify that the lab has negative pressure. Notify facilities
if beeping resumes. UNSAFE POWER LINES: if you see frayed or entangled power lines alert the person responsible for this section of the lab. CIMS TURBO PUMPS: if they are blinking red and/or have stopped spinning it probably means that the water circulator in the 4th is
low on water and stopped. Refill it with water following the SOP and restart the circulator and the pumps. CIMS DIAPHRAGM PUMPS: if they are noisier than usual or appear to have stopped turn the turbo pumps off by pressing on the
button in the bottom right corner of the front panel, and turn off the diaphragm pumps. Alert Sergey. WATER LEAKS: if you see water leaking from the lab water circulation lines, turn off laser, CIMS turbos, and booster pump in the
3rd floor chase immediately. UPS FIALURES: if the uninterruptable power supply is beeping, reset it and notify Sergey. It is time to replace the battery. CHILLERS/HEATERS: if you see water or ethanol flowing for the circulating bath heaters/coolers, turn them off immediately!
Safety Officer Responsibilities:
o The safety officer (Student Representative or SR) serves as a conduit of information among the campus safety organizations, group members, and the principle investigator.
o The specific responsibilities include: Completing the Laboratory Hazard Identification checklist annually and helping to ensure that identified hazards are corrected and
addressed. Helping group members create Standard Operating Procedures (SOPs). Ensuring that group members complete all required training. Help coordinate work unit specific training for new and existing members as necessary. Maintain written records for the above (i.e. the Safety Binders).
o Additionally, the safety officer will periodically check the laboratories for potential safety hazards, but the ultimate responsibility for safety will be the individual performing the work!!!
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Disposing of hazardous waste
o Liquid chemical waste - each hood has liquid chemical waste bottles that you can deposit waste into. Make sure to note the difference between aqueous waste and organic waste. There are a few types of organic waste bottles.
o **Common solvent waste** - common solvents used for washing like acetone has its own waste bottle. These types of wastes are to be redistilled with a Rotary Evaporator (bath temp ~ 30 C) to recycle the clean solvent and then only used for washing.
o Solid chemical waste – these types of waste should be dissolved into the solvent it is compatible with and disposed in the liquid waste. o Solid non-chemical hazardous waste – solids contaminated with chemicals but does not dissolve (e.g., silica gel) needs its own waste
container that EH&S will supply and pick up separately. o Solid non-chemical non-hazardous waste – these go in the trash. o Glass waste and non-glass waste that needs to be incinerated go into the glass container. o Do not put bottle from drinks into the glass container. Sergey hates it!!!o Razors and other sharps go into the sharps container. o Batteries go into the battery container to be recycled.
Important websites
Guide: go to a computer and show how to access these websites, go over what they are used for.Orientee: write down the main function
o Group website ______________________________________________________________________________________________
o Intranet and group chemical inventory __________________________________________________________________________
o Chemical inventory of UCI Physical Sciences (cheminnovation) ______________________________________________________
o PS Stores inventory __________________________________________________________________________________________
o Any online sign-up sheets ______________________________________________________________________________________
o Scifinder, Web of Science _____________________________________________________________________________________
o NIST chemical webbook ______________________________________________________________________________________
o CRC Chemical handbook ______________________________________________________________________________________
o Important journals like ACP, ES&T, etc. ____________________________________________________________________________
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Important seminars
o Group seminar (weekly on dates posted on group website in RH 390)– mandatory o AirUCI seminar (biweekly on Tuesday at 12 pm in RH 390) – mandatory o Pchem seminar (weekly on Tuesdays at 4 pm in RH 401) – mandatory when there is a talk relevant to atmospheric science o ESS seminar – keep an eye on their seminar program, some of them are very useful
Hands-on training to be provided
This will not happen in one day, but the following training should be provided to new lab members:
o Purchasingo Vacuum fittings – hands one training on how to use different types of fitting s and how to distinguish and use different types of tubingo Pumps – explaining how to service a vanes pump and a diaphragm pump, and change pump oil, the first time it is neededo Flow control – calibrating mass flow controllerso Humidity control – explaining how to deal with Nafion humidifiers and RH probleso Mounting hardware – showing how different mounts work:
Optical mounts Mechanical clamps Unistrut
o Electrical Soldering and isolating 3-phase, 2-phase, and 1-phase power sources in the lab
o SMPS – learning how to operate, service, download data, and plot datao Flow tube – learning how to operate and serviceo MAGIC – learning how to operate and serviceo SSSS – learning how to operate and serviceo Chamber – learning how to operate and serviceo UV/Vis – learning how to operate, service, download data, and plot datao PTR-MS – learning how to operate, service, download data, and plot datao AMS – learning how to operate, service, download data, and plot data
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