Global Local at promoters of genes

Same nucleus

ChIPexperiments

Differential

Taverna et al. 2007

chromodomain

chromodomain

Dominant negative

Taverna et al. 2007

Histone/chromatin crosstalk

appears in mitosis but not interphase.

appears in mitosis but not interphase.

Chromatin / Nucleosome dynamics

Nucleosome folding /packaging regulatesaccessibility of DNA templates (nucleosomal DNA and

linker DNA)

The dynamics of chromatin structure are regulatedthrough multiple mechanisms including:

--histone variant incorporation--histone posttranslational modifications (PTMs)

--ATP-dependent chromatin remodeling --histone chaperones--architectural chromosomal proteins

Tessarz and Kouzarides (2014)

Cell cycle regulated

* *

Results

Conclusions

Jack et al (2013)

Tessarz and Kouzarides (2014)

Lysine

Tessarz and Kouzarides (2014)

Nucleosome lateral surfacemodifications

-may modulate histone-DNAinteractions --> nuc stability --> nuc position stability ->increased mobility =increased accessibility

PTMs may affectnucleosome structures and

dynamics

Tessarz and Kouzarides (2014)

Nucleosomehistone-histonemodifications

--may destabilizetetramer-dimerinteractions --> nucstability --> histoneexchange

Tessarz and Kouzarides (2014)

Nucleosome facemodifications

--may modulatechromatin-bindingprotein interactionspositively / negatively

--may modulate nuc-nuc interactions -->higher order chromatinstructure

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+ EFFECTORS (dynamic interactions)