lecture 9 site specific recombination and transposition
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Lecture 9 Site Specific Recombination and Transposition. Quiz 5 due today at 4 PM. 12_Figure01.jpg. Examples of site specific recombination and transposition. Different than homologous recombination. “jumping gene”. Responsible for important DNA rearrangements - PowerPoint PPT PresentationTRANSCRIPT
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Lecture 9 Site Specific Recombination and Transposition
Quiz 5 due today at 4 PM
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Examples of site specific recombination and transposition
Different than homologous recombination
“jumping gene”
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• Responsible for important DNA rearrangements
CSSR = recombination between 2 defined sites
CSSR can be used to control gene expression.DNA inversion can allow an alternative gene to be expressed.
Transposition = recombination between specific sequences and non-specific DNA sites
Transposition is a (the) major source of spontaneous mutation.
Nearly half the human genome is transposon-derived sequences.
Conservative site specific recombination (CSSR) and transposition
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Certain viruses use site specific recombinationto integrate into the host chromosomeExample: lambda phage
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3 Types of conservative site specific recombination (CSSR)
A, B, X, and Y denote specific genes.The recombinase recognition sequences are dark orange and blue.Gray regions with white arrows are the crossover regions.
Direct repeats Direct repeats Inverted repeats
2 sites on different DNA molecules 2 sites on the same DNA molecule
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Structures involved in CSSR
Example of an insertion(orange DNA is usually circular)
Recombinase recognitionsequences are symmetric
Crossover region is assymetric
4 subunits of recombinase bind
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Synaptic complex
2 types of recombinasesSerine recombinase and tyrosine recombinase
Both use a covalent protein-DNA intermediate
Conservative in CSSR refers to energy; No ATP needed
Recombinasebinds 5’- phosphate
Leaves free 3’-OH tobind another DNA strand
Links DNA to3’-OH on new strand
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Mechanism of serine recombinase
Makes double stranded breakthat is staggered by 2 bases
All 4 strands cleaved beforestrand exchange
5’- phospho-serine and free 3’-OH formed.
R2 segment recombines withR3 segment
R4 segment recombines withR1 segment
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Mechanism of tyrosine recombinase
Break and rejoin one DNA strand at a time. R1 and R3 break and rejoin segments first.
3’- phospho-tyrosine and free 5’-OH formed.
A Holliday junction is formed.
R2 and R4 segments then recombine using the same mechanism to resolve junction.
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Phage P1 Cre recombinaseis a tyrosine recombinasesometimes used in genetic engineering
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Homologous recombination duringDNA replication of circular chromosomescan generate circular multimers.
CSSR is needed to resolve these multimersback into the monomers.
These recombinases are called resolvases.
XER tyrosine recombinase (resolvase)is an example from bacteria
Animation
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Transposable elements or transposons
Little selectivity in site selection. Can insert in the middle of genes. Less than 2% of human transposons code for proteins.
Non-replicative (cut & paste) Replicative (copy & paste)
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Some organisms (yeast, fruitflies, and E. Coli) contain few transposons.
Occurrence of transposons (green) in genomes
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12_Figure18.jpg 3 classes of transposons
(LTR retrotransposons)
(Long terminal repeat)(Reverse transcriptase)
RNA bindingenzyme
RT & endonuclease/ Rnase H
Non-viralretrotransposons
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DNA transposons: Cut &
paste mechanism of transposition
Old host site is grayNew host site is blueDouble strand break atold site must be repaired
Staggered cleavage ofnew target site DNA
New DNA synthesis givesrise to target site duplication
(transpososome)
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DNA strandtransfer3’-OH of transposon attacksphosphate in DNA to leave anew 3’-OH for polymeraseto add onto for the gap to befilled. Then ligase seals nick.
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Transposase only cleaves one strand. 3 ways to cleave nontransferred strand (5’-end) before strand transfer
A different endonuclease(TnsA)
3’-OH on trnspsn 3’-OH on host DNA
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DNA transposons: Replicative “copy & paste” transposition mechanism
Transposase nicks DNA to createthe 3’-OH at each end for strand transfer(same as in cut & paste)
3’-OH on each end cleavesand binds target DNA
DNA replication machinery then useseach 3’-OH on target DNA as a primerand copies the transposon
This leaves a large circular DNAmolecule containing 2 copies ofthe transposon
Animation
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Virus-like retrotransposonsuse an RNA intermediatebut otherwise use the samemechanism as in DNA transposition
Integrated virus
Virus uses an integrase related to DNA transposases.Integrase cleaves 2 nucleotides from the 3’-end, which is then used for DNA strand transfer.
Viruses need to use a special mechanismto regenerate the ends of the LTRs so they can be recognized by integrase.
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Target-site-primed reverse transcriptionof Poly-A (non-LTR) retrotransposonsHuman transposon LINEs (long interspersednuclear elements) use this mechanism.
LINE DNA transcribed. mRNA exported from nucleus.ORF1 and ORF2 proteins are translated from mRNA,remain attached to 3’-end of transcript, and transport mRNA back into the nucleus where itassociates with T- rich DNA by use of the poly-A tailTo form a DNA:RNA hybrid.
ORF2 (RT and endonuclease) nicks DNA generatinga 3’-OH for reverse transcription of RNA back into DNA that integrates into host DNA. DNA joining and repair establish the newly produced LINE element.
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Genetic organization of a LINE and SINE
20 % of the human genome is LINE elements (example L1).LINEs donate the proteins to replicate SINEs (100-400 bpsequences that make up 13 % of human genome). The Alu sequence is a SINE. Pseudogenes can be made by LINE proteins mistakingly binding and reverse transcribing normal mRNA.
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