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PHYTOCHEMICAL ANALYSIS
Nature of analysis Method
EXTRACTION OF PHYTOCHEMICALS Extraction Water Solvents Soxhlet extraction Water Solvents
Phytochemical analysis QUALITATIVE ANALYSIS
Phytochemical screening 10to 12 Tests Paper chromatography 2 to 4 solvents
TLC 4 solvents
Column chromatography Silica
Sephadex G OR DEAE cellulose
ANTIOXIDANT ACTIVITY UV Visible DPPH UV Visible
Hydrogen peroxide UV Visible FRAP UV Visible
Flavanoids/ Tannin UV Visible Total Phenol / Alkaloids UV Visible
Metal chelating UV Visible In vitro alpha amylase inhibition
assay UV Visible
In vitro α-glucosidase inhibition assay
UV Visible
Total falvonids
ANTIMICROBIAL ACTIVITY Per plate Anti fugal activity Per plate
Antimicrobial activity by TLC TLC plate HPLC for plant sample HPLC
HPTLC Compound identification GC MS With Library and
compound identification Anti cancerous activity
Using cell lines (one cell line)
Tryphan blue assay MTT Assay
DNA Damage activity Per sample Two cell line On request
Nature of analysis Method Nanoparticle synthesis
Types Gold Silver Zinc Copper Others
Characterization UV- VIS UV – VIS FTIR FTIR SEM SEM SEM AND EDX SEM and EDX
XRD XRD
Molecular Biology/ Recombinant / DNA finger Printing techniques
Nature of analysis Method DNA Gel electrophoresis ( Bacteria, Fungi or plant) With Gel doc
AGE
RNA AGE RFLP AGE RAPD ( Bacteria) - RAPD (Plant) ISSR AGE RAPD (Animal) ISSR AGE
Transformation - PCR alone Sequencing up to ~700 bases 16SRNAor 18 or 23sRNA
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Gene cloning and selection of clones TA Primers (per nucleotide)
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PROTEIN
Nature of analysis Method Salt ppt/ Dialysis/ and column chromatography
Down stream
Column chromatography Sephadex G OR DEAE cellulose
SDS- PAGE – with marker SDS Bio reductase assay SDS TLC Silica Gel HPLC for compound analysis HPLC GC alone GC
Anti cancerous/ Cyototoxicity assay
Nature of analysis Method
Using cell lines (one cell line) Tryphan blue assay
MTT Assay DNA Damage activity Per sample
Water/ Food analysis
Nature of analysis Method Food adulteration ( 5 to 6) UV- Visible /
Titration Microbial load (TVC) - Coli form test MPN and all the coliforms tests - Detection of Salmonella/ Shigella - Detection of Histamines HPLC Pathogens identification - Ash - Crude fiber Carbohydrate Fat Protein Fatty acids by TLC Fatty acids by GC-MS Aminoacids TLC Vitamin A Vitamin C/Ascorbic acid Folic acid TLC analysis of Vitamins
Mineral Analysis Iron UV Calcium UV Magnesium UV Potassium AAS Zinc AAS HPLC - GC MS - Please contact : 9629388728