lexa hybrid
DESCRIPTION
LexA Hybrid. Gal4AD Hybrid. -His. 1. CD4c C394R/V395D. Gal4AD. 2. CD4c C394R/V395D. Lck. 3. CD4c C394R/V395D. Siva-1. 4. CD4c C397R. Gal4AD. 5. CD4c C397R. Lck. 6. CD4c C397R. Siva-1. - PowerPoint PPT PresentationTRANSCRIPT
LexA
Hybrid
Gal4AD
Hybrid
Gal4AD
Siva-1
Lck
CD4c C394R/V395D
CD4c C394R/V395D
CD4c C394R/V395D
CD4c C397R
CD4c C397R
CD4c C397R Siva-1
Lck
Gal4AD
1
6
5
4
2
3
-His
Figure legend: L40 expressing CD4c C394R/V395D or CD4c C397R mutants fused to LexA, and Siva-1or Lck fused to Gal4AD or Gal4AD alone was analyzed for histidine auxotrophy. Both mutants are able to interact with Siva-1, demonstrating that the two cysteine residues C394 and C397 are not critical for the interaction of CD4c with Siva-1.
Figure S1