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Life Science Reagents for DNA/RNA Electrophoresis from Sigma-Aldrich®

We Ripple Through Everything You Do!• Extensive reagent offering to support your Nucleic Acid Electrophoresis research needs• Market leading manufacturer of biological buffers• Quality and availability assured• Market leading Technical Service • Leading Web support tools: PCR Product Guide — sigma-aldrich.com/pcrguide

Molecular Biology — sigma-aldrich.com/genomics Virtual Stockroom — sigma-aldrich.com/virtualstockroom

Proteomics

ProteinDetectionWestern Blots

& ELISAs

Cell-BasedAssaysCell

Signaling

RecombinantProtein Expression

& Purification

ProteinElectrophoresis

PAGE & 2D PAGE

Cloning

Nucleic AcidPreparation

DNA & RNA

Transfection CellCulture

Nucleic AcidAmplificationPCR, QPCR, RT-PCR Stem Cell

Culture

Genotyping

Nucleic AcidBlottingDNA & RNA

(Southern) (Northern)

Nucleic AcidElectrophoresis

DNA & RNA

Nuclear Magnetic Resonance (NMR)

2 sigma-aldrich.com For more information, please contact Sigma-Aldrich Customer Service at 800-325-3010.

Improved safety and speed with Sigma Electrophoresis products!

• Bionic™ Buffer - Resolve bands in minutes - Run gels 2–3× faster than TBE/TAE - Sharper bands in less time - Voltage less limited by heat generation - Allows for higher voltage runs

• BlueView™ Nucleic Acid Stain - Safe quick alternative to Ethidium Bromide - Visible in ambient light; avoid possible nicking

caused by UV - Bands can be excised and used for PCR, ligation,

labeling, southern blots, and restriction digests

SYBR® Green II for high sensitive RNA gel staining• Works with agarose and polyacrylamide gels

• Denatured and non-denatured gels

• Superior performance over ethidium bromide

• No washing or destaining

• Does not interfere with transfer to membranes for hybridization

For all your Molecular Biology needs, please visit sigma-aldrich.com/genomics.

Cat. No. DescriptionAcids & Bases320099 Acetic acid ACS reagent, ≥99.7%H1758 Hydrochloric acid Molecular Biology Grade, 36.5-38.0%

P5958 Potassium hydroxide SigmaUltra, ≥85% as KOHS8045 Sodium hydroxide SigmaUltra, ≥98%, pellets (anhydrous)

AcrylamidesA3553 Acrylamide for electrophoresis, ≥99%, powderA4058 Acrylamide solution 40%, for electrophoresis, sterile-filteredM7279 N,N'-Methylenebisacrylamide for electrophoresis, ≥98%, powder

AgaroseA5304 Agarose for electrophoresisA9539 Agarose for molecular biologyA4718 Agarose High resolution, DNase, RNase, NICKase, none detectedA5093 Agarose Low EEO, for molecular biologyA6013 Agarose Type I, Low EEOA0169 Agarose Type I-A, Low EEOA6877 Agarose Type II, Medium EEOA9793 Agarose Type III-A, High EEOA2576 Agarose Type IX-A, Ultra-low Gelling TemperatureA0701 Agarose Type VII-A, Low Gelling TemperatureA7431 Agarose Wide range/Standard 3:1, DNase, RNase, NICKase,

none detected

BuffersB6185 Bionic BufferB4429 BIS-TRIS Biotechnology Performance Certified, cell culture tested,

insect cell culture tested, ≥98%B6032 BIS-TRIS hydrochloride ≥98%B4679 BIS-TRIS propane Biotechnology Performance Certified,

cell culture tested, ≥99.0%B7901 Boric acid for electrophoresisM3183 MOPS Biotechnology Performance Certified, cell culture tested,

≥99.5%M9024 MOPS sodium salt Biotechnology Performance Certified,

cell culture tested, ≥99.5%M1442 MOPS solution Biotechnology Performance Certified, cell culture

tested, 1 M

P1851 PIPES Biotechnology Performance Certified, cell culture tested, ≥99%

S2889 Sodium acetate anhydrous for molecular biology

S8388 Sodium acetate solution 0.3 M for molecular biology

S3014 Sodium chloride for molecular biology, ≥98%

S6316 Sodium chloride solution 5 M, Biotechnology Performance Certified, cell culture tested

T9650 Tris Acetate-EDTA buffer 10× Concentrate, DNase and RNase, none detected

T4415 Tris-Borate-EDTA buffer 10× ConcentrateT1503 Trizma® base Primary Standard and Buffer, ≥99.9% T6066 Trizma base Biotechnology Performance Certified, meets EP,

USP testing specifications, cell culture tested, ≥99.9%

Life Science Reagents for Nucleic Acid Electrophoresis from Sigma-Aldrich®

3Delivering Science through Business Understanding

Cat. No. DescriptionT5941 Trizma hydrochloride for molecular biology, Biotechnology

Performance Certified, cell culture tested, ≥99.0%

General ReagentsA3678 Ammonium persulfate for electrophoresis, ≥98%C4654 Cytidine powder, cell culture tested

D9163 DL-Dithiothreitol for electrophoresis, ≥99%D9779 DL-Dithiothreitol for molecular biology, ≥98% (TLC), ≥99%

(titration)E5134 EDTA disodium salt dihydrate for molecular biology, ≥99%F2637 Ficoll®, for molecular biology, lyophilized powderF8775 Formaldehyde solution for molecular biology, 36.5-38% in H2OF5786 Formamide SigmaUltra, ≥99%G5516 Glycerol for molecular biology, ≥99%G6264 Guanosine cell culture testedG8773 Glycerol for electrophoresis, ≥99%

L9650 Lithium chloride for molecular biology, ≥99%S0389 Sucrose for molecular biology, ≥99.5%T0625 Taurine ≥99%

T9281 TEMED for electrophoresis, ~99%W3513 Water Biotechnology Performance CertifiedW4502 Water Molecular Biology Reagent

U6504 Urea for electrophoresis

Loading Buffers and Tracking DyesB3269 Bromphenol Blue–Xylene Cyanole Dye solution lyophilized powderB5525 Bromophenol Blue sodium salt for electrophoresisC8468 ChromaTrack™ Dye Marker Mix for Nucleic Acid Agarose GelsG2526 Gel Loading Buffer RNAse, none detectedG7654 Gel Loading Solution Type IR4268 RNA Sample Loading Buffer With ethidium bromide (50 μg/mL)R1386 RNA Sample Loading Buffer Without ethidium bromideX4126 Xylene Cyanol FF for molecular biology

Molecular Weight MarkersD0428 1 kb Ladder buffered aqueous solutionD5042 123 bp Ladder buffered aqueous solutionC8468 ChromaTrack Dye Marker Mix for Nucleic Acid Agarose GelsD3937 DirectLoad™ 1 kb DNA Ladder aqueous Ficoll solution

with bromophenol blue and xylene cyanol, 1 vial sufficient for 100 loads

D3687 DirectLoad PCR 100 bp Low Ladder aqueous glycerol solution with orange G and xylene cyanol

D3812 DirectLoad Step Ladder, 50 bp (Supplied in loading buffer, ready for use in agarose gels.)

D7058 DirectLoad Wide Range DNA Marker aqueous Ficoll solution with bromophenol blue and xylene cyanol

D5292 DNA Ladder, Supercoiled buffered aqueous solutionP1473 PCR 100 bp Low Ladder buffered aqueous solutionP1598 PCR 20 bp Low Ladder buffered aqueous solutionD7808 PCR Low Ladder SetP9577 PCR Marker solution in loading buffer

Cat. No. DescriptionR6895 Transcript RNA Markers 0.1-1 kb buffered aqueous solution

R0889 18S + 28S Ribosomal RNA from calf liver aqueous solution

R7644 Transcript RNA Markers 0.28-6.6 kb buffered aqueous solution

R7020 Transcript RNA Markers 0.2-10 kb buffered aqueous solutionS7025 Step Ladder, 50 bp buffered aqueous solution

SolventsI9516 2-Propanol for molecular biology, ≥99%

179124 Acetone ACS reagent, ≥99.5%

E7023 Ethanol 200 proof (absolute), for molecular biology

F9037 Formamide for molecular biology, ≥99.5%

494437 Methanol biotech. grade, 99.93%

Stains & DyesA6014 Acridine Orange hemi(zinc chloride) salt for molecular biologyB5525 Bromophenol Blue sodium salt for electrophoresis

P9172 Pyronin Y RNA staining tested

S9305 SYBR® Green II RNA gel stain 10,000× in DMSOT8935 BlueView™ Nucleic Acid Stain in 10× TAE bufferT9060 BlueView Nucleic Acid Stain in 10× TBE bufferE1510 Ethidium bromide solution 10 mg/mL, for molecular biology,

aqueous solutionE1385 Ethidium bromide solution for molecular biology, 500 μg/mL in

H2O, suitable for gel electrophoresis and DNA isolation proceduresM9140 Methylene blue certified by the Biological Stain CommissionX4126 Xylene Cyanol FF for molecular biology

EquipmentZ710067 Gel XL Ultra V-2™ electrophoresis system complete with 115 V

power supply and casting sets with combsZ710113 Gel XL-100™ electrophoresis system standard gel casting set Z356042 Horizontal IBI electrophoresis units Model MP 1015, MultipurposeZ338818 Midi horizontal electrophoresis unitZ338796 Mini horizontal electrophoresis unit Complete set

Mini horizontal electrophoresis unit (Cat. No. Z338796) Designed for rapid, routine electrophoresis for nucleic acids.

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Order/Customer Service (800) 325-3010 • Fax (800) 325-5052

Technical Service (800) 325-5832 • sigma-aldrich.com/techservice

Development/Bulk Manufacturing Inquiries (800) 244-1173

©2008 Sigma-Aldrich Co. All rights reserved. SIGMA, , SAFC, , SIGMA-ALDRICH, ALDRICH, , FLUKA, , and SUPELCO, are trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P. Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip. Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. ChromaTrack, DirectLoad is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Ficoll is a registered trademark of GE Healthcare. Gel XL Ultra V-2 and Gel XL-100 are trademarks of Labnet Intl., Inc.

Nucleic Acid Electrophoresis and Blotting

Agarose and AcrylamideUsed in routine electrophoresis of nucleotides for DNA identification, Southerns, Northerns, or DNA recovery. Sigma offers a wide range of agarose products depending on the desired result, such as low melting point, high resolution, or pulse-field gels. Acrylamide is used to perform Polyacrylamide Gel Electrophoresis (PAGE) gels for protein or high-resolution nucleic acid electrophoresis.

Precast GelsTo speed up your electrophoresis workflow Sigma offers a variety of precast gels for nucleic acid electrophoresis. The precast gels have a variety of concentrations and wells available to run gels specific to the needs of each experiment.

Denhardt's SolutionMixture of blocking agents used in hybridization protocols such as Northerns and Southerns to prevent non-specific binding of probes.

DNA Ladders and MarkersSigma has an assortment of nucleic acid markers for use in agarose or PAGE electrophoresis. The range and number of bands in each ladder varies depending on the size of nucleotide for identification. Ladders are also optimized for use at specific gel concentrations.

Dyes and Loading BuffersDyes, such as Xylene Cyanol and Bromophenol Blue are added to nucleic acid samples to track the migration of samples as agarose or PAGE electrophoresis occurs. Sigma also provides Gel Loading Buffers that contain components such as sucrose to facilitate sample loading.

Nucleic Acid Gel StainsEthidium bromide is an intercalation agent used to identify nucleotides after conducting electrophoresis. When placed inside a UV light box ethidium bromide fluoresces enabling identification of samples.

Running BuffersBuffers such as Tris-Borate-EDTA (TBE) are most commonly used for PAGE gels while Tris-Acetate-EDTA (TAE) is used for agarose electrophoresis. Sigma has a broad portfolio of Running Buffers depending on the specific running conditions. Bionic Buffer enables gels to be run at higher voltages resulting in runtimes that are 2–3 times faster than TBE or TAE.

ssDNAUsed to prevent non-specific binding of probes to membranes when Northern or Southern blots are performed.

EquipmentSigma-Aldrich has an assortment of equipment used in electrophoresis and blotting, of which gel boxes, power supplies, and pipette tips are available. Barrier pipette tips are used to prevent sample contamination.

Learn more at sigma-aldrich.com/genomics.