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ELECTRONIC SUPPLEMENTARY MATERIAL

Title: Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides

Journal: Antonie van Leeuwenhoek

Hemant Kumar Rawata, Mohd Anis Ganaieb and Naveen Kangoa*aDepartment of Applied Microbiology and Biotechnology, Dr. Harisingh Gour University, Sagar (M.P.) India bLabratório de Flavor e Análises Cromatográficas, Universidade Federal de Sergipe, Brazil

Email address: nkango@gmail.com

Figure captions

Fig 1 (a-g) HPLC chromatograms showing the standards of Inulin (GFn), Kestose (GF2), Nystose (GF3),

Fructofuranosylnystose (GF4), Sucrose (GF), Fructose (F) and Glucose(G)

Fig 2 HPLC chromatogram showing the mix standard of sugars.

Fig 3A (a-h) HPLC Chromatograms of end product formed by intracellular and extracellular sucrase action of

selected fungal strains

Fig 3 B (a-f) HPLC Chromatograms of end product formed by intracellular and extracellular FTase action of

selected fungal strains

Fig 4 A (a-h) HPLC Chromatograms of end product formed by intracellular and extracellular exoinulinase action of

selected fungal strains

Fig 4 B (a-h) HPLC Chromatograms of end product formed by extracellular exoinulinase action of selected fungal

strains

Fig 4 C (a-d) HPLC Chromatograms of end product formed by intracellular and extracellular endoinulinase action

of selected fungal strains

(Incubation time 24 h, pH- 5.0, 50oC)

(Abbreviations: F-fructose, G-Glucose, GF-Sucrose, GF2-Kestose, GF3-Nystose, Gfn or s-fructan or inulin; I-

intracellular, E-extracellular)

Fig 1 (a) Inulin (GFn)

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Fig 1 (b) Kestose (GF2)

Fig 1 (c) Nystose (GF3)

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Fig 1 (d) Fructofuranosylnystose (GF4)

Fig 1 (e) Sucrose (GF)

Fig 1 (f) Glucose (G)

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Fig 1 (g) Fructose (F)

Fig 2 Mix standards of sugars

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Aspergillus awamori MTCC 2879 (I) Aspergillus awamori MTCC 2879 (E)

Aspergillus ficuum MTCC 7591 (I) Aspergillus ficuum MTCC 7591 (E)

Fig. 3 A (a-f)

ba

d

fe

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Aspergillus niger GNCC 2613 (I) Aspergillus niger GNCC 2613 (E)

Aspergillus niger GNCC 2655 (I) Aspergillus niger GNCC 2655 (E)

hg

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Penicillium citrinum MTCC 1256 (I) Penicillium citrinum MTCC 1256 (E)

Penicillium rugulosum MTCC 3487 (I) Penicillium rugulosum MTCC 3487 (E)

Fig 3 B (a-f)

b

c

a

d

f

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Aspergillus niger ATCC 26011 (I) Aspergillus niger ATCC 26011 (E)

Aspergillus awamori MTCC 2879 (I) Aspergillus awamori MTCC 2879 (E)

Aspergillus niger GNCC 2655 (I) Aspergillus niger GNCC 2655 (E)

Aspergillus ficuum MTCC 7591 (I) Aspergillus ficuum MTCC 7591 (E)

Fig 4A (a-h)

c

b

d

e f

c d

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Aspergillus niger GNCC 2613 (I) Aspergillus niger GNCC 2613 (E)

hg

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Arthrinium pucciniodes NFCCI 2432 (E) Kluyveromyces marxianus MTCC 3995 (E)

Fusarium oxysporum NFCCI 2429 (E) Pichia guillermondii MTCC 1311(E)

Aspergillus niger GNCC 1420 (E)Aspergillus sp. GNCC 2608 (E)

Fig 4B (a-h)

a

d

f

h

e f

c

g

ba

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Fig 4C (a-d)

Penicillium rugulosum MTCC 3487 (E)Aspergillus versicolor GNCC 2608 (E)

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Penicillium NFFCI 2768 (I) Penicillium NFFCI 2768 (E)

Penicillium citrinum MTCC 1256 (I) Penicillium citrinum MTCC 1256 (E)Table 1 Details of habitats of isolation of native fungal strains used in the study

S. No. Fungal strains Habitat of isolation

1 Arthrinium puccinoides NFCCI 2432 Dahlia rhizospheric soil2 Aspergillus flavus NFCCI 2364 Sugarcane rhizospheric soil 3 Aspergillus fumigatus GNCC1351 Decaying dandelion root 4 Aspergillus niger GNCC 2613 Decaying onion peel

5 Aspergillus niger GNCC 1059 Decaying garlic peel

6 Aspergillus niger GNCC 1328 Decaying garlic peel

7 Aspergillus niger GNCC 1420 Decaying onion peel

8 Aspergillus niger GNCC 2655 Decaying banana peel 9 Aspergillus ochraceous GNCC 1423 Decaying asparagus root

10 Aspergillus versicolor GNCC 2608 Dandelion rhizospheric soil

11 Aspergillus sp. GNCC 2413 Decaying garlic peel

12 Aspergillus sp. GNCC 2418 Decaying onion peel

13 Aspergillus sp. GNCC 2531 Decaying onion peel

14 Aspergillus terreus NFCCI 2347 Sunflower rhizospheric soil

15 Fusarium oxysporum NFCCI 2429 Garlic rhizospheric soil16 Penicillium sp. NFCCI 2768 Dahlia rhizospheric soil