LisaHelblingChadwick,PhD

Na6onalIns6tuteofEnvironmentalHealthSciences

ProgramDirector,4DNucleome

Epigenome(2005-2015)

Genome(1990-2005)

Ajourneyfromgenometonucleome

Epigenome(2005-2015)

Genome(1990-2005)

Ajourneyfromgenometonucleome

4DNucleome(2015-2020)

The4DNucleomeProgramseekstoadvanceunderstandingof:

•  Theprinciplesunderlyingnuclearorganiza3onofmammaliangenomesinspaceand3me

•  Therolethatnuclearorganiza3onplaysingeneexpressionandcellularfunc3on

•  Howchangesinnuclearorganiza3onaffectnormaldevelopmentanddisease

Expecteddeliverables•  Nextgenera6ontoolstoexploretherela>onshipbetweengenome

organiza>onandfunc>on–bothmoleculartoolsandimagingtools

•  Referencemapsofthe3Dorganiza>onofthegenomeinavarietyofhumancells/>ssuesandcellstates.

•  Developmentofcommunitystandardsandmetrics•  Greaterunderstandingofpoorly-characterizednuclearstructures

andnuclearbodies:roleingenomeorganiza>on&func>on.

•  Approachestointegratemolecularinforma6onwithimaginginforma6on

From:DarrowEMetal.,

PNAS2016

BuildingtheNetwork

Grantsawardedinsummer2015

Technologydevelopmentanddataproduc>ontounderstand3Dstructure/func>onrela>onships

Developmentoftoolsandtechnologiestoinves>gatethestructureandfunc>on

ofnuclearbodies/domains

Developmentandvalida>onofnoveltechnologiestoinves>gate

thethree-dimensionalorganiza>onofthegenome

Developmentandvalida>onofnovelimagingtechnologiesforvisualizingthestructureandfunc>onofthegenome,andhowitchanges

Data/metadatacollec>on,cura>on,anddissemina>on

Programcoordina>on,outreach,opportunity

funds

www.4dnucleome.org

www.4dnucleome.org

Applica6onsdueJanuary27,2017!

Transforma6veCollabora6veProjectAward

4DNWorkingGroupMembersCo-chairs:RodericPeTgrew,MD,PhD NIBIBDinahS.Singer,PhD NCIPhilSmith,PhD NIDDK

WorkingGroupCoordinators:OlivierBlondel,PhD NIDDKRichardConroy,PhD NIBIBJudyMietz,PhD NCI

CommonFundProgramLeaders:AnandaRoy,PhD OSC

4DNProgramDirector:LisaHelblingChadwick,PhD NIEHS

4DNProgramAnalyst:IddilBekirov,PhD NIDDK

WorkingGroup:DavidBalasundaram,PhD CSRTerryBishop,PhD NIDDKAnthonyCarter,PhD NIGMSSeanHanlon,PhD NCIRebeccaLenzi,PhD OSCMikePazin,PhD NHGRILisaPostow,PhD NHLBIMa]Reilly,PhD NIAAARobertRiddle,PhD NINDSJohnSa]erlee,PhD NIDAGeethaSenthil,PhD NIMHJessicaSmith,PhD OSCJoseM.Velazquez,PhD NIA