LITR GFP RITR

LITR GFP RITRER

E1 E3

E1 E3

AdGFP

AdER

A

AdER + E2 AdGFP + E2

AdER No Ligand AdGFP No Ligand

MCF-72,000 particles/cell

B

CD

Supplementary Figure 1. Transduction of MCF-7 cells with an adenovirus encoding human ERα.(A) Shown are diagrammatic representations of the genomes of AdGFP and AdERα, which are derived from pAdTrack-CMV. (B) MCF-7 cells transduced with AdGFP or AdERα were analysed for percentage of cells that were infected 48 hours following infection, by FACS for GFP-positive cells. Shown are the results of three transduction experiments; error bars represent the standard errors of the mean (SEM). (C) MCF-7 cells were infected using MOI = 2,000. Shown are phase contrast pictures of MCF-7 and MLET5 cells, together with fluorescence images for detection of GFP (magnification x40). (D) Cell growth was determined using the SRB assay and the results represent the mean spectrophotometric values from three experiments, each carried out in triplicate. (E) MLET5 lines arose from AdERα-infected MCF-7 cells, selected after 8 months of culturing in estrogen-free conditions. Shown are phase contrast pictures of MCF-7 and MLET5 cells, together with fluorescence images for detection of GFP (magnification x40).

E