logo twinkle medicine hunter of cancer lzu-china igem team 2013
TRANSCRIPT
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Twinkle Medicine Hunter Of Cancer
LZU-China iGEM Team 2013
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Content
12
3
4Introduction
Experimental
HumanPractice
Results & Discussion
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Background: About Cancer Estimated Number of Persons Alive in the U.S.
Diagnosed with Cancer in 2006 (N = 11.4 M survivors)
Estimated Number of Female Cancer Survivors in the U.S. in 2006 (N = 6.2 M survivors)
Chap.1 Chap.2 Chap.3 Chap.4
Estimated Number of Persons Alive in the U.S. Diagnosed with Canceron January 1, 2006 by Time From Diagnosis and Gender(Invasive/1st Primary Cases Only, N = 11.4 M survivors
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Background: About Cancer
Chap.1 Chap.2 Chap.3 Chap.4
What cause cancer?Cancer cells come from normal cells, what changed?
Gene mutation
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Background: Overview of NF-κB classical signaling pathway
Activated NFkB were found in almost 70% cancer cells.
NFkB signaling pathway has become a variety of malignant tumor therapy and drug discovery is an important target.
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Background: Overview of NF-κB classical signaling pathway
NF-κB Signaling Pathway
FADD
IκB-αNF-κB
p65/50
NF-κBp65/50
IκB-αcIAP1/2Bcl-xL/2SurvivinICAM-1Cyclin-D1VEGF
Gene expression
Cytoplasm
PKC
Nucleu
IKKs
PMA
TNF-α IL-1TNFR IL-1R
MEKKS
TRADD
RIPTRAFs
TRAF6TAK1
MyD88
IRAK
P
P
P
IκB-αUb P
Proteasome
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Background: Negative feedback loop of NF-κB
Chap.1 Chap.2 Chap.3 Chap.4
Negative feedback loop
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Background: Goal
How to
Changethe situation
Chap.1 Chap.2 Chap.3 Chap.4
Traditional : LOW accuracy Mice live short/
Can’t take long-term detection
?
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Experimental: Device
Promoter:4 copies of κB
Binding sequence
Chap.1 Chap.2 Chap.3 Chap.4
IκB+GFP Terminator
Pref
Suff
pSB1C3
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Experimental: Design Cancer Why IκBα exists?
Chap.1 Chap.2 Chap.3 Chap.4
NF-κB signaling pathway feedback loop dynamis
To last a long term detection Sacrifice less mice
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Experimental: Device
Promoter:4 copies of κB
Binding sequence
Chap.1 Chap.2 Chap.3 Chap.4
IκB+GFP Terminator
Pref
Suff
pSB1C3
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Background: Goal
Chap.1 Chap.2 Chap.3 Chap.4
NF-κB signal is inhibited.
Oscillation represents drug’s duration time.
Flu
ores
cent
ligh
t
Flu
ores
cent
ligh
t
Time Time
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Experimental: Results & Discussion: Results
Chap.1 Chap.2 Chap.3 Chap.4
DU-145
IκBα
GAPDH
shRNA- IκBα - +
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Experimental: Results & Discussion: Results
Chap.1 Chap.2 Chap.3 Chap.4
DU145 Cell
DU-145
IκBα
GAPDH
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Experimental: Results & Discussion: Future Goals
1. Making our drug screening system work better.
1. Improve specifity: building the fine-adjusted in other cells.2. Shorten twinkle cycle-time: show some features in pharmacokinetics of drugs.3. Achieve semiquantitative measure: detect the light intensity of GFP and make a standard curve.
Chap.1 Chap.2 Chap.3 Chap.4
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Experimental: Results & Discussion: Application
1. Making our drug screening system work better.
1. How to do: inject DU-145 in which have a moderate expression level of IκB.2. Long-term drug evaluation system: tumor grow slowly, mice survive longer.3. Sensitive system: observe twinkle instead o measuring the size of tumor.
Chap.1 Chap.2 Chap.3 Chap.4
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Experimental:
Chap.1 Chap.2 Chap.3 Chap.4
Human Practice
Our team had a interchange with Peking iGEM team and BIT iGEM team in August. We shared our own idea to each other, and offered advice for the problem.
Our Team’s member Li Xiaomeng had been honoured to visit the BIT iGEM 2013 Team’s lab. And help them to characterize and construct a part of Cr sensor.
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Experimental: Results & Discus
Chap.1 Chap.2 Chap.3 Chap.4
Human Practice
We held two promotion meetings concerning iGEM for all students in our school and Lanzhou No.1 Meddle School successfully. Meanwhile, the opening day of iGEM lab has been excellently work out with the amazing enthusiasm in this summer vacation.
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Acknowledgements
Ph.D Jinbo Yang, Huyuan Feng & Their Labs
Institute of Cancer Biology and Drug Screening
of Lanzhou University
School of Life Science of Lanzhou University
Chap.1 Chap.2 Chap.3 Chap.4
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THANKS!