loschmidt laboratories enantis, ltd. protein engineering
TRANSCRIPT
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Loschmidt Laboratories
Enantis, Ltd.
PROTEIN ENGINEERING
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LOSCHMIDT LABORATORIES & ENANTIS
17 years experience in protein science
multi-disciplinary team
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LOSCHMIDT LABORATORIES & ENANTIS
17 years experience in protein science
multi-disciplinary team
laboratories and facilities (1.5 mil EUR)
University Campus and Biotechnology Incubator, Brno
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LOSCHMIDT LABORATORIES & ENANTIS
17 years experience in protein science
multi-disciplinary team
laboratories and facilities (1.5 mil EUR)
University Campus and Biotechnology Incubator, Brno
molecular biology and microbiology
![Page 5: Loschmidt Laboratories Enantis, Ltd. PROTEIN ENGINEERING](https://reader036.vdocument.in/reader036/viewer/2022062321/56649f1f5503460f94c3731f/html5/thumbnails/5.jpg)
LOSCHMIDT LABORATORIES & ENANTIS
17 years experience in protein science
multi-disciplinary team
laboratories and facilities (1.5 mil EUR)
University Campus and Biotechnology Incubator, Brno
molecular biology and microbiology
bioinformatics and molecular modelling
![Page 6: Loschmidt Laboratories Enantis, Ltd. PROTEIN ENGINEERING](https://reader036.vdocument.in/reader036/viewer/2022062321/56649f1f5503460f94c3731f/html5/thumbnails/6.jpg)
17 years experience in protein science
multi-disciplinary team
laboratories and facilities (1.5 mil EUR)
University Campus and Biotechnology Incubator, Brno
molecular biology and microbiology
bioinformatics and molecular modelling
biochemistry and biophysics
LOSCHMIDT LABORATORIES & ENANTIS
![Page 7: Loschmidt Laboratories Enantis, Ltd. PROTEIN ENGINEERING](https://reader036.vdocument.in/reader036/viewer/2022062321/56649f1f5503460f94c3731f/html5/thumbnails/7.jpg)
LOSCHMIDT LABORATORIES & ENANTIS
BIOSENSING
DECONTAMINATION
BIOCATALYSIS
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DEVELOPMENT OF BIOCATALYTIC PROCESS
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GENETIC DATABASES
EXTREMOPHILES
DEVELOPMENT OF BIOCATALYTIC PROCESS
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DIRECTED EVOLUTION
resistance to organic solvents
DEVELOPMENT OF BIOCATALYTIC PROCESS
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DIRECTED EVOLUTION
RATIONAL DESIGN
thermostability enhancement
DEVELOPMENT OF BIOCATALYTIC PROCESS
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DIRECTED EVOLUTION
RATIONAL DESIGN
thermostability enhancement
DEVELOPMENT OF BIOCATALYTIC PROCESS
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DIRECTED EVOLUTION
RATIONAL DESIGN
thermostability enhancement
0
0.2
0.4
0.6
0.8
1
1.2
30 40 50 60 70 80
Nat
ive
stru
ctur
e
Temperature [°C]
20°C
DEVELOPMENT OF BIOCATALYTIC PROCESS
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OPTIMIZED EXPRESSION
FERMENTATION
DOWN STREAM
21.5
29.0
35.4
52.9
92.0113.0
1 2 3 4
DhaA-LinB
DEVELOPMENT OF BIOCATALYTIC PROCESS
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MEDIUM ENGINEERING
IMMOBILISATION
DEVELOPMENT OF BIOCATALYTIC PROCESS
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HOTSPOT WIZARD
Hot Spot Wizard
RCSB PDB
user
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Hot Spot Wizard
RCSB PDB
CSAUniProt
user
PDBSWS
HOTSPOT WIZARD
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Hot Spot Wizard
RCSB PDB
CSAUniProt
user CAVER
CASTp
PDBSWS
HOTSPOT WIZARD
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Hot Spot Wizard
RCSB PDB
CSAUniProt
user CAVER
CASTp
PDBSWS
tunnels
Cd-hit
BLAST NRDB
MUSCLERate4Site
HOTSPOT WIZARD
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low highmutability
HOTSPOT WIZARD
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1,3-1,4--glucanase PDB-ID 1GBG
haloalkane dehalogenasePDB-ID 1BN6
phosphotriesterasePDB-ID 1I0D
-lactamasePDB-ID 1BLS
HOTSPOT WIZARD
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Estimated mutability
hot spotsregion
Pro
po
rtio
n o
f m
uta
tio
ns
HOTSPOT WIZARD
positive and neutral
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Estimated mutability
hot spotsregion
Pro
po
rtio
n o
f m
uta
tio
ns
HOTSPOT WIZARD
positive and neutral slightly negative
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positive and neutral slightly negative deleterious
Estimated mutability
hot spotsregion
Pro
po
rtio
n o
f m
uta
tio
ns
HOTSPOT WIZARD
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HOTSPOT WIZARD
Pavelka et al. Nucl. Acids Res. 37: W376 2009