Lox Site Lox Site

Random Non-coding DNA (1-2kb)

Stop Codons

Antibiotic Resist

Cre Pops BlockerCre Pops Blocker

This device will prevent Pops from a promoter reaching target genes. Activation of the Cre Recombinase genes will remove the stop codons and allow Pops to pass through this part. Removal of the stop codons also allows transcription of an antibiotic so the activated bacteria can be selected. The restriction sites allow the part to be ligated into a system in the normal fashion.

This part can only be activated once and will remain activated forever. It should be 100% efficiant at stopping Pops passing through it until activated. No other molecules are required for any of the steps, simple.

A separate induceable Cre plasmid must be inserted to produce the cre recombinase

Antibiotic Resist

How Cre WorksHow Cre Works

Cre is under the control of a promoter, there are commercially available Cre containing plasmids

How to make this in 2 hrs How to make this in 2 hrs

Lox Site

Lox Site Stop Codons

Random DNAAntibiotic Resistance

Restriction sites

Restriction sites

Primer 1Primer 2 Primer 3

(Low Concentration)

Random DNA Antibiotic Resistance

John Chattaway and Jonny Wells