lymphocytes and aryl hydrocarbon hydroxylase activity

421 9 Comparison of rat and guinea pig as sources of the 89 fraction in the Salmo- nella/mammalian microsome test ROBERT S.U. BAKER, TONY M. BONIN, IEVA STUPANS and GERALD M. HOLDER A highly significant enhancement of mutagenicity occurs with 11 polycyclic aromatic hydrocarbons (PAH) when 3-methylcholanthrene (MC)-induced guinea pig liver $9 is substituted for Aroclor-induced rat-liver $9 in the Ames test. The use of MC-induced guinea pig-liver $9 is particularly valuable for detecting the weak mutagenicity of benz[c]acridine, which is barely positive in a standard Ames assay. However, anthracene and phenanthrene, which are generally considered not to be carcinogens, remain non-mutagenic for strain TA100. This enhancement of mutagenicity does not correlate with arylhydro- carbon hydroxylase (AHH) activities of the various liver preparations and does not apply to certain other non-PAH mutagens, including fl-naphthylamine, afiatoxin BI and 4-dimethylaminoazobenzene. 10 Lymphocytes and aryl hydrocarbon hydroxylase activity JOHN COULTER Lung cancer is not an inevitable consequence of smoking and the question arises whether this is due entirely to random targeting in an homogenous population or due partly to genetically determined differences. Kerrerman's work on aryl hydrocarbon hydroxylase induction in lymphocytes using a fluorescent assay purported to show that differences existed and were related to susceptibil- ity to lung cancer although others have failed to confirm these results. The present paper reports the results of testing induced lymphocytes as a source of activating enzymes in a Salmonella typhimurium mutagenicity test. It has been found that lymphocytes contain both activating and deactivating enzymes which make this approach unsatisfactory for easy population testing. 11 Sister-chromatid exchanges in the lymphocytes of humans on long-term lithium therapy NUREL Z. LATIMER, E. CHIN and O.M. GARSON Sister-chromatid exchanges (SCE) revealed by differential staining of human lymphocyte chromosomes grown for 2 generations in 5-bromo-2'-deoxyuridine

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Page 1: Lymphocytes and aryl hydrocarbon hydroxylase activity

421

Comparison of rat and guinea pig as sources of the 89 fraction in the Salmo- nella/mammalian microsome test

ROBERT S.U. BAKER, TONY M. BONIN, IEVA STUPANS and GERALD M. HOLDER

A highly significant enhancement of mutagenicity occurs with 11 polycyclic aromatic hydrocarbons (PAH) when 3-methylcholanthrene (MC)-induced guinea pig liver $9 is substituted for Aroclor-induced rat-liver $9 in the Ames test. The use of MC-induced guinea pig-liver $9 is particularly valuable for detecting the weak mutagenicity of benz[c]acridine, which is barely positive in a standard Ames assay. However, anthracene and phenanthrene, which are generally considered not to be carcinogens, remain non-mutagenic for strain TA100. This enhancement of mutagenicity does not correlate with arylhydro- carbon hydroxylase (AHH) activities of the various liver preparations and does not apply to certain other non-PAH mutagens, including fl-naphthylamine, afiatoxin BI and 4-dimethylaminoazobenzene.

Lymphocytes and aryl hydrocarbon hydroxylase activity

JOHN COULTER

Lung cancer is not an inevitable consequence of smoking and the question arises whether this is due entirely to random targeting in an homogenous population or due partly to genetically determined differences. Kerrerman's work on aryl hydrocarbon hydroxylase induction in lymphocytes using a fluorescent assay purported to show that differences existed and were related to susceptibil- ity to lung cancer although others have failed to confirm these results. The present paper reports the results of testing induced lymphocytes as a source of activating enzymes in a Salmonella typhimurium mutagenicity test. It has been found that lymphocytes contain both activating and deactivating enzymes which make this approach unsatisfactory for easy population testing.

Sister-chromatid exchanges in the lymphocytes of humans on long-term lithium therapy

NUREL Z. LATIMER, E. CHIN and O.M. GARSON

Sister-chromatid exchanges (SCE) revealed by differential staining of human lymphocyte chromosomes grown for 2 generations in 5-bromo-2'-deoxyuridine

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