madhuca part 3

8/13/2019 Madhuca Part 3

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4.2.4: Quantitative microscopy

Quantitative microscopical values like stomatal index, vein islet number, vein termination

number and palisade ratio of the two varieties are given in Table VI. Stomatal index value

and palisade ratio of var. latifoliais higher than var. longifolia. Vein islet number and vein

termination number are higher in var. longifoliathan that in var. latifolia.

4.2.5: Analytical values

Analtical values like total ash, acid!insoluble ash, loss on dring, solubilit percentage in

alcohol and water and extractive values in petroleum ether, ben"ene, chloroform, alcohol

and water of stem bark and corolla of two varieties of M. longifolia are given in Table VII.

Ash value of stem bark is higher in var. longifoliathan that of var. latifolia. Ash value of

corolla is higher in var. latifolia. #oss on dring is lower in var. longifolia in both stem

barks and corolla. $or stem bark, solubilit percentage in alcohol is higher in both

varieties whereas for corolla solubilit percentage in water is higher. The varieties show

di%erence in their solubilit percentage of both bark and corolla lobes. &xtractive values

of bark and corolla lobes also reveal di%erences. &xtractive value of bark in petroleum

ether and alcohol are signi'cantl higher in var. latifolia. &xtractive values of corolla

lobes of var. latifoliain ben"ene are three folds higher.

4.2.6: Study of powder(ehavior of powdered bark and corolla of two varieties on treatment with di%erent

chemical reagents and )uorescent behavior are given in Table VIII, I*, * and *I. It shows

both similarities and dissimilarities.

4.2.7: Qualitative phytochemical studies

Qualitative phtochemical analsis for alkaloids, carbohdrates, tannins and phenols,

)avonoids, gums and mucilage+s, 'xed oils and fats and saponins of bark and corolla of

two varieties ofM. longifolia are given in Table *II and *Ill respectivel. The following

distinct analtical di%erences were observed. (en"ene and water extracts of stem bark of

var. longifoliaare brown and powder, whereas, in var. latifolia, ben"ene extract is ellow

and sand and water extract are deep red and powder. (en"ene extract of corolla is

brown and oil in var. longifoliabut ellow oil in var. latifolia. Qualitative phtochemical

tests of two varieties show both similarities and dissimilarities Table *II and *Ill-.

4.2.8: Quantitative phytochemical analysis

Tannins and phenols content in stem bark of var. latifoliais higher /0 mg1gm and 23

mg1gm respectivel- than that of var. longifolia450 mg1gm and 20 mg1gm respectivel-.


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Sucrose as invert sugar- in corolla is higher in var. longifolia22.667- than that in var.

latifolia38.827-.

4.2.: !haracters of "il from seed #ernels

In general, kernel of var. latifoliaields more oil percentage than that of var. longifoliainall the solvents used hexane, chloroform and petroleum ether-. 9exane is the best solvent

than the others in extracting maximum percentage of oil from kernels Table *IV-.

Analtical standards of oil like refractive index, speci'c gravit, color of the oil and

iodine value are more or less similar in the two varieties. 9owever, saponi'caion value is

slightl higher in var. latifoliathan that in var. longifoliaTable *IV-.


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$a%le &': Quantitative microscopical values of leaves of two varieties ofM.

longifolia

Sl. (o )arameters Studied var. longifolia var. latifolia

*Stomatal index

:pper surface

#ower surface

;A ;o Stomata-

/.


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$a%le &'': Analytical values ,in percenta-e of stel/l %ar# and corolla of two

varieties ofM longifolia

Sl.

;o=arameter studied

(ark >orolla

var. longifolia var.latifolia var. longifolia var. latifolia

4 Total ash value 8.<


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$a%le0&''': 1ehavior of powdered stem %ar# of two varieties ofM. longifolia on

treatment with dierent chemical rea-ents.

Sl.

(o

)owder 3 ea-ent used var. longifolia var. latifolia

* =owder as such @eddish brown @eddish brown

2 = >one. 9SB3 (rown @eddish brown

+ = >one. 9;0/ Cellowish red Cellowish red

4 = >one. 9>I Dark brown Dark brown

5 = Acetic acid (lackish brown (lackish brown

6 = I07 ;aB9 @eddish black @eddish black

7 = 4; 9>I @ed (rown

8 = Iodine Solution (rownish blue (rownish blue

= 2 7 $erric chloride (lack (lack


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$a%le0': 1ehavior of powdered corolla of two varieties ofM. longifolia on

treatment with dierent chemical rea-ents.

Sl.

(o

)owder 3 ea-ent used var. longifolia var. longifolia

I =owder as such (lack (lack

= >onc. 9SB3 @eddish brown @eddish brown

/ = >onc. 9;0/ @eddish brown @eddish black

3 = >onc. 9>I @ed @eddish brown

2 = Acetic acid (rown (rown

< = I0 7 ;aB9 (rownish ellow Cellow

8 = 4; 9>I @ed @ed

6 = Iodine Solution (rown (rown

5 = 2 7 $erric chloride (lack (lack


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$a%le : luorescent %ehavior of powdered stem %ar# of two varieties ofM

longifolia

Sl.

(o

)owder 3

ea-ent used

var.longifolia var.latifolia

&isi%le li-ht & li-ht &isi%le li-ht & li-ht

* =owder as such @eddishbrown

(rown @eddishbrown

(rown

2 = 207 9;0/(rownish

black

(rownish

black

(rownish

black(lack

+ = 4; 9>ICellowish

brown

@eddish

brown

Cellowish

brownCellow

4= 4; ;aB9

in ?ater(rown Cellow (rown Cellow

5= 4; ;aB9 in

alcohol(rown

Cellowish

black(rown

Cellowish

black


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$a%le ': luorescent %ehavior of powdered corolla of two varieties ofM longifolia

Sl.

;o

=owder

@eagent used

var.longifolia var.latifolia

Visible

light:V light

Visible

light:V light

4 =owder as such (lack (lack (lack (lack

= 207

9;0/(rown

Ereenish

Cellow(rown Cellow

/ = 4; 9>I @ed @ed @ed@eddish

ellow

3= 4; ;aB9

in ?aterCellow

Cellowish

brownCellow

Cellowish

brown

2= 4; ;aB9

in alcoholCellow (rown Cellow (rown


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$a%le '': )reliminary phytochemical analysis of stem %ar# of two varieties ofM.

longifolia

.

Test for

@eagents :sed1

Fethods

adopted

&xtracts of

=etroleum ether (en"ene >hloroform Alcohol ?ate

Var.lon.

Var. lat. Var.lon.

Var. lat. Var.lon.

Var. lat. Var.lon.

Var. lat. Var.lon.

V

>olor G =hsical consistenc

Eolden

ellow

resinou

s stick

Eolden

ellow

resinou

s stick

(rown

powder

Cellow

sand

(rown

powder

(rown

sand

(right

G deep

red to

brown

powder

Deep

red

stick

(rown

powder

D

re

p

Alkaloids. =icric acid 33 3 33 0 0 3 0 3 3

Dragondro% 00 3 3 0 0 3 0 3 0

FaerHs 0 0 3 3 3 3 3 3 0

?agnerHs 3 0 33 3 3 3 3 3 0

>arbohdratesFolishHs 0 0 0 0 0 0 3 3 3

$ehlingHs 0 0 0 0 0 0 3 0 3

(enedicts 0 0 0 0 0 0 3 3 3

Tannins G $erric chloride 0 0 0 0 0 0 3 3 3=henols Eelatin 0 0 0 0 0 0 0 3 3

#ead acetate 0 0 0 0 0 0 3 0 3

$lavonoids ShinodaHs test 0 0 0 0 0 0 0 0 0

EumsG

Fucilage+s

Alcoholic

=recipitation0 0 0 0 0 0 0 0 0

$ixed oils G

fatsSpot test 3 3 0 0 0 0 0 0 0

Saponins $oam test 0 0 0 0 0 0 3 3 3

madhuca part 3

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