magnification and microscopes
TRANSCRIPT
Regents Biology
Microscopes!
Regents Biology
Mutual dependence: Science & Technology
Technology…the pragmatist
Does it work?
Is it useful?
Is it sustainable?
Science…the idealist
What is it?
How does it work?
What does it tell us?
Regents Biology
Mutual dependence: Science & Technology
Microscopes
Electron microscopes
Optical (light) microscopes
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Size matters micron = micrometer = 1/1,000,000 meter
diameter of human hair = ~20 microns
Miss N, Show them the interactive!!
Regents Biology
Microscopic units of measurement
Regents Biology
1 metre (m) = ________ millimetres (mm)
1 mm = ________ microns (µm)
1 µm = ________ nanometres (nm)
1 mm = ________ nm
1 m = ________ µm
1 m =________ nm
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1 m = 1,000 mm = 103mm
1 mm = 1,000 µm= 103µm
1 µm = 1,000 nm= 103nm
1 mm = 1,000,000 nm = 106nm
1 m = 1,000,000 µm = 106µm
1 m = 1,000,000,000nm =109 nm
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10-3 m = ________
10-6 m = ________
10-9 m = ________
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10-3 m = 0.001m = 1 mm
10-6 m = 0.000001 = 1 µm
10-9 m = 0.000000001= 1nm
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Electron microscope range:
_____ to _____
Light microscope range:
_____ to _____
Unaided human eye range:
_____ to _____Unaided?
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Electron microscope range:
1 nm to 100 µm
Light microscope range:
1 µm to 1 mm
Unaided human eye range:
1 mm upwards
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Name that image
Light microscope
or
Electron microscope?
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Regents Biology19 Light microscope
Unstained
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Regents Biology20 Light microscope
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Regents Biology22 Light microscope
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Tick
Regents Biology23 Electron microscope
Tick
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Regents Biology24 Electron microscope
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Regents Biology25 Light microscope
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Regents Biology26 Electron microscope
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Aphid
Regents Biology27 Electron microscope
Aphid
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Regents Biology28 Light microscope
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Regents Biology29 Light microscope
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Regents Biology30 Electron microscope
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Regents Biology31 Electron microscope
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Regents Biology32 Electron microscope
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Regents Biology33 Electron microscope
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Regents Biology35 Light microscope
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Regents Biology36 Light microscope
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Regents Biology37 Light microscope
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THE END!
Regents Biology
Magnification
Magnification = ocular lens x objective lens
A microscope has an eyepiece lens with a power
of 20X. The objective lens being used has a
power of 10X. What is the total magnification?
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Using Scale Bars
Using a scale bar:The image at right is of a virus-like
particle. The bar represents 50nm.
Calculate the actual size of the
image. 50nm
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1. Measure the scale bar
50nm=1cm
2. Measure the image
Let’s say it was 2cm.
Actual size: Image size = constant
50:1 = X:2
X=2*50
=100nm
Using a scale bar:
50nm
Using Scale Bars
Regents Biology
The image at right is of a virus-
like particle. The bar represents
50nm.
Calculate the magnification.
50nm
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Magnification: I AM
Watch yo units!!
Image size
Actual size Magnification
Measure with ruler Need to figure this out
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1. Measure the size of the image.
…..2cm (20mm)
2. Find the actual size of the sample
…..100nm Or 1.0 x 102
3. Convert measured size into unit on scale bar.
(in this case, nm)
mm µm 1mm = 1000 µm
20 x 1000µm =20,000 µm
µm nm 1 µm = 1000 nm
20,000 x 1000nm = 20,000,000nm OR 2.0 x 107nm
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Magnification: I AM
Watch yo units!!
Image size
Actual size Magnification
2.0 x 107Need to figure this out
1.0 x 102
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(2.0x107) / (1.0x102)
=2.0 x 10(7-2)
=2.0 x 105
x200,000 whoa50nm
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Magnification: I AM
Watch yo units!!
Image size
Actual size Magnification
2.0 x 107nm
X 2.0 x 105
1.0 x 102nm
Regents Biology
Using Microscopes