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TRANSCRIPT
Title: Infinium HD Assay Super Lab Tracking Form Part Number: 11327261
Revision: C Effective Date: 06/15/10
1 of 7
Template No: 11253484 Rev D Proprietary & Confidential—For Internal Use Only
1 Make and Read Quant (Optional/LIMS) Date/Time: ____________________________________________ WG#-DNA Plate: QNT Plate: Operator: ___________________ Robot: ___________________ (1) ___________________ _____________________ (2) ___________________ _____________________ (3) ___________________ _____________________
Standard DNA Plate: _____________________
Standard QNT Plate: _____________________
2 Make MSA1 Date/Time: ____________________________________________ WG#-DNA Plate: ____________________________ Operator: ___________________ Robot: ___________________ MSA1 Plate: ________________________________ Plate Positions on Robot Bed: ___________________________ MA1 Reagent: ______________________________ Batch #: _______________________________________________ MA2 Reagent:_______________________________ Number of Samples: 48 / 96 MSM Reagent: ______________________________ Hyb oven (37°C, 20-24 h): Start __________ Stop __________ Record WG#-DNA sample IDs in table on page 3.
3 Fragment MSA1 Date/Time: ____________________________________________ FMS Reagent: _______________________________ Operator: ___________________ Robot: ___________________ Plate Positions on Robot Bed: ___________________________ Vortex at 1600 rpm Heat block (37°C, 1 h): Start ___________ Stop ____________
4 Precip MSA1 Date/Time: ____________________________________________ 2-propanol Lot #: _____________________________ Operator: ___________________ Robot: ___________________ 2-propanol Date Opened: _____________________ Plate Positions on Robot Bed: ___________________________ PM1 Reagent: ________________________________ Vortex at 1600 rpm Heat block (37°C, 5m): __________________________________
Incubate (4°C, 30m): Start: ____________ Stop: ____________
Air dray (22°C, 1 h): Start: ____________ Stop: _____________
5 Resuspend MSA1 Date/Time: ____________________________________________ RA1 Reagent: _______________________________ Operator: ___________________ Robot: ___________________ Plate Positions on Robot Bed: ___________________________ Hyb oven (48°C, 1 h): Start ___________ Stop _____________
6 Hyb Multi BC2 Date/Time: ____________________________________________ PB2 Reagent: ________________________________ Operator: ___________________ Robot: ___________________ ________________________________
Project: _______________ Batch: _______________ M
SA1 Plate: _______________ Im
age Date: _______________
Title: Infinium HD Assay Super Lab Tracking Form Part Number: 11327261
Revision: C Effective Date: 06/15/10
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Template No: 11253484 Rev D Proprietary & Confidential—For Internal Use Only
Vortex at 1800 rpm ________________________________ Heat block (95°C, 20m): Start: ___________ Stop: __________
Refer to pages 4–6 for BeadChip loading instructions. Enter the BeadChip barcodes in the spaces provided.
Centrifuge MSA1 plate to 280 xg Hyb oven (48°C, 16-24 h): Start _________ Stop ___________
7 Wash BeadChip Date/Time: ____________________________________________ PB1 Reagent: ________________________________ Operator: ___________________ Robot: ___________________ ________________________________ ________________________________
8 XStain HD BeadChip Date/Time: ____________________________________________ RA1 Reagent: ________________________________ Operator: ___________________ Robot: ___________________ XC1 Reagent: (1-8) ___________________________
Record the chamber rack position for each BeadChip on page 7. (9-16) __________________________ (17-24) _________________________ XC2 Reagent: (1-8) ___________________________ (9-16) __________________________ (17-24) _________________________ TEM Reagent: (1-8) ___________________________ (9-16) __________________________ (17-24) _________________________ XC3 Reagent: ________________________________ STM Reagent: (1-8) ___________________________ (9-16) __________________________ (17-24) _________________________ STM Temperature: ____________________________ ATM Reagent: (1-8) ___________________________ (9-16) __________________________ (17-24) _________________________ PB1 Reagent: ________________________________ XC4 Reagent: ________________________________
9 Image BeadChip Record the Scanner ID and the image date for each BeadChip on pages 4–6.
Title: Infinium HD Assay Super Lab Tracking Form Part Number: 11327261
Revision: C Effective Date: 06/15/10
3 of 7
Template No: 11253484 Rev D Proprietary & Confidential—For Internal Use Only
Record DNA Sample IDs in the MSA1 Plate Columns 1–4 of Microtiter Plate
1 2 3 4
A ______________________________ ______________________________ ______________________________ ______________________________
B ______________________________ ______________________________ ______________________________ ______________________________
C ______________________________ ______________________________ ______________________________ ______________________________
D ______________________________ ______________________________ ______________________________ ______________________________
E ______________________________ ______________________________ ______________________________ ______________________________
F ______________________________ ______________________________ ______________________________ ______________________________
G ______________________________ ______________________________ ______________________________ ______________________________
H ______________________________ ______________________________ ______________________________ ______________________________
Columns 5–8 of Microtiter Plate
5 6 7 8
A ______________________________ ______________________________ ______________________________ ______________________________
B ______________________________ ______________________________ ______________________________ ______________________________
C ______________________________ ______________________________ ______________________________ ______________________________
D ______________________________ ______________________________ ______________________________ ______________________________
E ______________________________ ______________________________ ______________________________ ______________________________
F ______________________________ ______________________________ ______________________________ ______________________________
G ______________________________ ______________________________ ______________________________ ______________________________
H ______________________________ ______________________________ ______________________________ ______________________________
Columns 9–12 of Microtiter Plate
9 10 11 12
A ______________________________ ______________________________ ______________________________ ______________________________
B ______________________________ ______________________________ ______________________________ ______________________________
C ______________________________ ______________________________ ______________________________ ______________________________
D ______________________________ ______________________________ ______________________________ ______________________________
E ______________________________ ______________________________ ______________________________ ______________________________
F ______________________________ ______________________________ ______________________________ ______________________________
G ______________________________ ______________________________ ______________________________ ______________________________
H ______________________________ ______________________________ ______________________________ ______________________________
Title: Infinium HD Assay Super Lab Tracking Form Part Number: 11327261
Revision: C Effective Date: 06/15/10
4 of 7
Template No: 11253484 Rev D Proprietary & Confidential—For Internal Use Only
Track BeadChips 1-24 for the 4x1 HD BeadChip
MSA1 Plate
WG
1123
4567
89-M
SA
1
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
OddNumberedBeadChips
EvenNumberedBeadChips
R03C01
R01C01
1123456789
R02C01
R04C01
Sample Section Naming Diagram
Barcode:
Scanner ID:
Image Date:
A3
B3
C3
D3
BC2 #5
BC2 #1
A1
B1
C1
D1
E3
F3
G3
H3
BC2 #2
BC2 #6
E1
F1
G1
H1
A4
B4
C4
D4
BC2 #7
BC2 #3
A2
B2
C2
D2
E4
F4
G4
H4
BC2 #4
BC2 #8
E2
F2
G2
H2
A5
B5
C5
D5
BC2 #9
E5
F5
G5
H5
BC2 #10
A6
B6
C6
D6
BC2 #11
E6
F6
G6
H6
BC2 #12
Robot Setup #1
BC2 #13
A7
B7
C7
D7
BC #14
E7
F7
G7
H7
BC2 #15
A8
B8
C8
D8
BC2 #16
E8
F8
G8
H8
A9
B9
C9
D9
BC2 #17
BC2 #21
E9
F9
G9
H9
BC2 #18
BC2 #22
BC2 #19
BC2 #23
BC2 #20
BC2 #24
A11
D11B11
C11 E11 G11
F11 H11
E12
F12
G12
H12
E10
F10
G10
H10
A10
B10
C10
D10
A12
B12
C12
D12
Robot Setup #2
1123456789 1123456789 1123456789
1123456789
1123456789
1123456789
11234567891123456789
1123456789
1123456789
1123456789
1123456789
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
1123456789
1123456789
1123456789
1123456789
1123456789
1123456789
1123456789
1123456789
1123456789
1123456789
1123456789
1123456789
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Title: Infinium HD Assay Super Lab Tracking Form Part Number: 11327261
Revision: C Effective Date: 06/15/10
5 of 7
Template No: 11253484 Rev D Proprietary & Confidential—For Internal Use Only
Track BeadChips 1-24 for the 4x3 HD BeadChip
MSA1 Plate
WG
1123
4567
89-M
SA
1
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
OddNumberedBeadChips
EvenNumberedBeadChips
R 0 1 C 0 2R 0 1 C 0 1
1123456789
R 0 2 C 0 2R 0 2 C 0 1
Sample Section Naming Diagram
Barcode:
BeadArrayReader ID:
Image Date:
A3
B3
C3
D3
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #5
1123456789 610Qv1HD
1123456789 610Qv1HD
BC2 #1
A1
B1
C1
D1
E3
F3
G3
H3
Barcode:
BeadArrayReader ID:
Image Date:
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #2
BC2 #6
1123456789 610Qv1HD
1123456789 610Qv1HD
E1
F1
G1
H1
Barcode:
BeadArrayReader ID:
Image Date:
A4
B4
C4
D4
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #7
1123456789 610Qv1HD
1123456789 610Qv1HD
BC2 #3
A2
B2
C2
D2
E4
F4
G4
H4
Barcode:
BeadArrayReader ID:
Image Date:
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #4
BC2 #8
1123456789 610Qv1HD
1123456789 610Qv1HD
E2
F2
G2
H2
A5
B5
C5
D5
Barcode:
BeadArrayReader ID:
Image Date:1123456789 610Qv1HD
BC2 #9
E5
F5
G5
H5
Barcode:
BeadArrayReader ID:
Image Date:1123456789 610Qv1HD
BC2 #10
A6
B6
C6
D6
Barcode:
BeadArrayReader ID:
Image Date:1123456789 610Qv1HD
BC2 #11
E6
F6
G6
H6
Barcode:
BeadArrayReader ID:
Image Date:1123456789 610Qv1HD
BC2 #12
Robot Setup #1
Barcode:
BeadArrayReader ID:
Image Date:1123456789 610Qv1HD
BC2 #13
A7
B7
C7
D7
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #14
1123456789 610Qv1HD
E7
F7
G7
H7
Barcode:
BeadArrayReader ID:
Image Date:1123456789 610Qv1HD
BC2 #15
A8
B8
C8
D8
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #16
1123456789 610Qv1HD
E8
F8
G8
H8
A9
B9
C9
D9
Barcode:
BeadArrayReader ID:
Image Date:
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #17
1123456789 610Qv1HD
BC2 #21
E9
F9
G9
H9
Barcode:
BeadArrayReader ID:
Image Date:
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #18
1123456789 610Qv1HD
1123456789 610Qv1HD
BC2 #22
Barcode:
BeadArrayReader ID:
Image Date:
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #19
1123456789 610Qv1HD
1123456789 610Qv1HD
BC2 #23
Barcode:
BeadArrayReader ID:
Image Date:
Barcode:
BeadArrayReader ID:
Image Date:
BC2 #20
1123456789 610Qv1HD
1123456789 610Qv1HD
BC2 #24
A11
D11B11
C11 E11 G11
F11 H11
1123456789 610Qv1HD
E12
F12
G12
H12
E10
F10
G10
H10
A10
B10
C10
D10
A12
B12
C12
D12
Robot Setup #2
Title: Infinium HD Assay Super Lab Tracking Form Part Number: 11327261
Revision: C Effective Date: 06/15/10
6 of 7
Template No: 11253484 Rev D Proprietary & Confidential—For Internal Use Only
Track BeadChips 1-12 for the 8x1 HD BeadChip MSA1 Plate
WG
1123
4567
89-M
SA
1
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
R 0 1 C 0 1
R 0 2 C 0 1
R 0 3 C 0 1
R 0 4 C 0 1
R 0 5 C 0 1
R 0 6 C 0 1
R 0 7 C 0 1
R 0 8 C 0 1
Sample Section Naming Diagram
Barcode:
Scanner ID:
Image Date:
BC2 #5
BC2 #1 BC2 #2
BC2 #6 BC2 #7
BC2 #3 BC2 #4
BC2 #8
BC2 #9 BC2 #10 BC2 #11 BC2 #12
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
Barcode:
Scanner ID:
Image Date:
1123456789
A1B1
E1F1
C1D1
G1H1
1123456789
A3B3
E3F3
C3D3
G3H3
1123456789
A2B2
E2F2
C2D2
G2H2
1123456789
A4B4
E4F4
C4D4
G4H4
1123456789
A5B5
E5F5
C5D5
G5H5
1123456789
A6B6
E6F6
C6D6
G6H6
1123456789
A7B7
E7F7
C7D7
G7H7
1123456789
A8B8
E8F8
C8D8
G8H8
1123456789
A9B9
E9F9
C9D9
G9H9
A11
1123456789
B11
C11D11
G11H11
E11F11
A12
1123456789
B12
C12D12
G12H12
E12F12
A10
1123456789
B10
C10D10
G10H10
E10F10
Title: Infinium HD Assay Super Lab Tracking Form Part Number: 11327261
Revision: C Effective Date: 06/15/10
7 of 7
Template No: 11253484 Rev D Proprietary & Confidential—For Internal Use Only
Chamber Rack Position Chart Use this chart to enter BeadChip IDs in the appropriate chamber rack position during the XStain HD BeadChip step.
Row 1 Row 2 Row 3
1
5
2
6
3
7
4
8
9
13
10
14
11
15
12
16
17
21
18
22
19
23
20
24
Release History Revision CO # Originator Description of Change
C 12952 E. Clark Incorporated new Illumina Template for this info.