manipulating the mous embryo - genes &...
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Manipulating the Mous Embryo A Laboratory Manual, Second Edition By Brigid Hogan, Vanderbilt University Medical School; Rosa Beddington, National Institute for Medical Research, London; Frank Costantini, Columbia University; Elizabeth Lacy, Memorial Sloan-Kettering Cancer Center
The 1986 publication of Manipulating the Mouse Embryo catalyzed the interaction between molecular biology and mammalian embryology. For the first time, detailed instructions on how to begin applying recombinant DNA technology to important questions about mammalian embryonic development were made available to a broad audience. The gather- ing pace of such studies in recent years has brought improvements to existing methods and fueled the creation of new and powerful technologies. The sec- ond edition of this classic manual has been complete- ly revised and expanded to incorporate these ad- vances. It contains new sections on the production and analysis of transgenic mice, the manipulation of preimplantation embryos to generate chimeras, the culture and manipulation of embryonic stem cells, in- eluding gene "knockouts," and techniques for visual- izing genes, gene products, and specific cell types. As before, included with the protocols is a summary of current understanding of mouse development at a molecular level. In its new edition, this manual of proven distinction is again an authoritative and com- prehensive source of technical guidance for experi- enced investigators and an essential resource for new- comers to mammalian genetics and embryology.
CONTENTS Developmental Genetics and Embryology of the Mouse: Past, Present, and Future Section A. Summary of Mouse Development Section B. Setting Up a Colony for the Production of Transgenic and Mutant Mice
Section C. Recovery, Culture, and Transfer of Embryos and Germ Cells Section D. In Vitro Manipulation of Preimplantation Embryos Section E. Production of Transgenic Mice Section F. Isolation, Culture, and Manipulation of Embryonic Stem Cells Section G. Analysis of Transgenic Mice Section H. Techniques for Visualizing Genes, Gene Products, and Specialized Cell Types Section I. In Vitro Culture of Eggs, Embryos, Primordial Germ Cells, and Teratocarcinoma Cells Appendices Suggested Reading References
1994, 497 pp., illus., color plates, appendices, glossary index Plastic comb binding $95 ISBN 0-87969-384-3 Cloth $190 ISBN 0-87969-392-4
Reader Service No. 318
GENES
DEVELOPMENT
VOLUME 9 NUMBER 4 PAGES 387-508 FEBRUARY 15, 1995
EDITORIAL BOARD
J. Adams (Melbourne, Australia) J. Beckwith (Boston, USA) A. Berns (Amsterdam, The Netherlands) E. Blackburn (San Francisco, USA) T. Cech (Boulder, USAI P. Chambon (Strasbourg, France) N.-H. Chua (New York, USA) E. Coen (Norwich, UK) S. Courtneidge (Heidelberg, FRG) R. Evans (La Jolla, USA) G. Fink (Cambridge, USA) P. Goodfellow (Cambridge, UK) S. Gottesman (Bethesda, USA) T. Graf (Heidelberg, FRG} C. Gross (San Francisco, USA) R. Grosschedl (San Francisco, USA} F. Grosveld (Rotterdam, The Netherlandsl M. Groudine (Seattle, USA) L. Guarente (Cambridge, USA) R. Harland (Berkeley, USA) E. Harlow (Charlestown, USA) W. Herr (Cold Spring Harbor, USA) J. Hodgkin (Cambridge, UK) R. Horvitz (Cambridge, USA) P. Ingham (London, UK) T. Jessell (New York, USA) N. Jones ILondon, UK)
Editorial~Production N. Dumser, Technical Editor V. Nicolette, Production Editor L. Olsewski, Editorial Secretary
J. Kadonaga (La Jolla, USA) R. Lehmann (Cambridge, USA) M. Levine (San Diego, USA} D. Livingston (Boston, USA) R. Losick (Cambridge, USA) J. Manley (New York, USA} W. McGinnis (New Haven, USA) S. McKnight (South San Francisco, USA) R. McKay {Bethesda, USA) A. McMahon (Cambridge, USA} P. Nurse (London, UK) R. Palmiter (Seattle, USA) L. Parada (Dallas, USA) C. Prives {New York, USA) G. Rubin (Berkeley, USA) U. Schibler (Geneva, Switzerland) P. Sharp (Cambridge, USA1 C. Sherr (Memphis, USA) D. Sherratt (Oxford, UK) D. Solter (Freiburg, FRG) P. Soriano (Seattle, USA) J. Steitz (New Haven, USA} M. Takeichi (Kyoto, Japan) T. Taniguchi (Osaka, Japan) S. Tilghman (Princeton, USA) R. Tjian (Berkeley, USA) M. Wigler (Cold Spring Harbor, USA)
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Contents GENES & DEVELOPMENT February 15, 1995
Research papers
The Cpx two-component signal transduction pathway of Escherichia coil regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP Paul N. Danese, William B. Snyder, Christine L. Cosma, Laura J.B. Davis, and Thomas J. Silhavy
387
G~ cyclin turnover and nutrient uptake are controlled by a common pathway in yeast Yves Barral, Stefan Jentsch, and Carl Mann
399
Reciprocal interferences between nucleosomal organization and transcriptional activity of the yeast SNR6 gene Marie-Claude Marsolier, Shigeo Tanaka, Magda Livingstone-Zatchej, Michael Grunstein, Fritz Thoma, and Andr6 Sentenac
410
Identification and characterization of genes that are required for the accelerated degradation of mRNAs containing a premature translational termination codon Ying Cui, Kevin W. Hagan, Shuang Zhang, and Stuart W. Peltz
423
Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen Feng He and Allan Jacobson
437
DNA rearrangements located over 100 kb 5' of the Steel (S/)-coding region in Steel-panda and Steel-contrasted mice deregulate SI expression and cause female sterility by disrupting ovarian follicle development Mary A. Bedell, Camilynn A. Brannan, Edward P. Evans, Neal G. Copeland, Nancy A. Jenkins, and Peter I. Donovan
455
Inhibition of viral gene expression by the catalytic RNA subunit of RNase P from Escherichia coli Fenyong Liu and Sidney Altman
471
RNA polymerase II subunit RPB9 is required for accurate start site selection Melissa W. Hull, Keith McKune, and Nancy A. Woychik
481
flbD encodes a Myb-like DNA-binding protein that coordinates initiation of Aspergillus n/du/ans conidiophore development Jenny Wieser and Thomas H. Adams
491
Cell--cell signaling pathway activating a developmental transcription factor in Bacillus subtilis J.-Arturo Londofio-Vallejo and Patrick Stragier
503
Cover Expression of Mgf mRNA in P12 ovaries SlP~n/S1 p~n mice. (For details, see Bedell et al., p. 455.)