mark a collins ph.d marketing manager, cellular imaging & analysis
DESCRIPTION
MIAHA - Minimum Information for a High Content Assay - a proposal for a standard for high content analysis data interchange. Mark A Collins Ph.D Marketing Manager, Cellular Imaging & Analysis. Review. Standard for Images and Data An image standard alone is not enough - PowerPoint PPT PresentationTRANSCRIPT
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MIAHA - Minimum Information for a High Content Assay - a proposal for a standard
for high content analysis data interchange
Mark A Collins Ph.DMarketing Manager, Cellular Imaging &
Analysis
2
Review
Standard for Images and Data• An image standard alone is not enough
• To analyse an image between different packages need more than just it’s type and format
How acquired Magnification Filters etc
• Need a standard for data interchange that uses appropriate meta data to describe HCS/HCA images and data
Self describing format of XML proposedEmbrace and extend two existing standards
• OME – Open Microscopy Environment• http://www.openmicroscopy.org/
• MIACA – Minimum Information for A Cellular Assay• http://miaca.sourceforge.net/
3
Practical Implementation of a Standard
Not just talk• Draft specification for HCS/HCA Data Interchange completed
• MIAHA Minimum Information for A High Content Assay
Testing the standard• HCSGateway™ Cellomics open interface to third party readers
• GE INCELL 1000/3000• Evotec Opera
Implemented the standard as part of the HCSGateway product• Get third party data in to our informatics• Get third party data out
4
Implementing a Standard – Generation 1
Cellomics Store database
Webservices
Instrument data & Images
MIAHA XML Document & image links
HCSGateway transform to
MIAHA
Webservices API natively “reads” and
writes MIAHA XML standard
3rd party Analysis software
5
Implementing a standard - Generation 2
Cellomics Store database
Webservices
MIAHA XML Document & image links
Webservices API natively “reads” and
writes MIAHA XML standard
Instrument/Analysis software directly
writes/reads MIAHA XML data
6
Current Standard Usage
Generation 2 Implementation• Evotec Opera• GeneData Screener• ArrayScan• CellWoRx
Generation 1 Implementation• INCELL 1000• INCELL 3000• Other custom
7
HCSGateway™ Open Instrument interface - implements MIAHA
HCSGatewayOpen Interface for Instrumentation
GE IN Cell Analyzer 1000
GE IN Cell Analyzer 3000
API CellWoRx*Reader
Cellomics ReaderFamily
Evotec Opera OtherInstruments