marketplace winter2012 q1 weur final

G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G

G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T

Molecular Biology MarketplaceThermo Scientific

A quarterly publication containing special offers for significant savings on a variety of molecular biology products.

High Quality Costs Less

Marketplace

Request a free copy from your local Fisher office

25% OFFThermo Scientific FastDigest Restriction Enzymes

GeneRuler DNA Ladders

NEW GeneJET Nucleic Acid Purification Kits

TurboFect Transfection Reagent Direct PCR Kits

qPCR Reagents and First Strand cDNA Synthesis Kits

Experience the power and convenience of FastDigest® Restriction Enzymes• One universal buffer for all 176 FastDigest enzymes• Complete digestion in 5-15 minutes• Direct loading on gels with FastDigest Green Buffer• 100% buffer compatibility with downstream applications

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Analyze your FastDigest results using GeneRuler DNA Ladders

Discover all 176 FastDigest Enzymes at www.thermoscientific.com/fastdigest

GeneRuler™ DNA Ladders• Ideal for both DNA sizing and approximate quantification • Sharp bands and bright reference bands• Supplied with additional 6x DNA loading dye for your sample DNA • Produced using individual chromatography-purified DNA fragments

r-t-u: ready to use / premixed with loading dye

GeneRuler 1 kb Plus DNA Ladder

0.5µg/lane, 8cm length gel, 1X TAE, 7V/cm, 45min

7000 20.010000 20.0

4000 20.0

700 25.0500 75.0

300 25.0

75 25.0

400 25.0

1000 25.0

2000 20.0

3000 20.0

5000 75.0

1500 80.0

20000 20.0

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bp ng/0.5µg

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*To redeem, place your order through Fisher Scientific using the specific promotional product codes detailed above. Offers are valid until March 31, 2012. Prices may vary through distributors.

*To redeem, place your order through Fisher Scientific using promotional code FastDigest_MP33 and receive 25% discount on your order of FastDigest enzymes. Offers are valid until March 31, 2012. Prices may vary through distributors.

Promo Code Product Quantity

SM0242-MP33 GeneRuler 100bp DNA Ladder 5x50 µg

SM0244-MP33 GeneRuler 100bp DNA Ladder, r-t-u 5x100 applic.

SM0322-MP33 GeneRuler 100bp Plus DNA Ladder 5x50 µg

SM0324-MP33 GeneRuler 100bp Plus DNA Ladder, r-t-u 5x100 applic.

SM0311-MP33 GeneRuler 1kb DNA Ladder 5x50 µg

SM0312-MP33 GeneRuler 1kb DNA Ladder 25x50 µg

SM0313-MP33 GeneRuler 1kb DNA Ladder, r-t-u 5x100 applic.

SM1331-MP33 GeneRuler 1kb Plus DNA Ladder 5x50 µg

SM1332-MP33 GeneRuler 1kb Plus DNA Ladder 25x50 µg

SM1333-MP33 GeneRuler 1kb Plus DNA Ladder, r-t-u 5x100 applic.

GeneJET™ Plasmid Midiprep KitGeneJET™ Plasmid Maxiprep Kit

High-quality plasmid DNA: A260/280 1.7 – 1.9.High yields and high concentration of purified DNA.• Midiprep – 200 μg, ≥ 400 ng/μL• Maxiprep – 750 μg, ≥ 400 ng/μL

Silica-based membrane technology.Centrifuge and vacuum manifold protocols.Purified DNA is ideal for transfection of robust cell lines.

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High yield of purified plasmid DNA

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GeneJET™ Whole Blood Genomic DNA Purification Mini Kit• Purify RNA-free genomic DNA • Fast 20-minute procedure with no separate cell lysis stepGeneJET™ Plant Genomic DNA Purification Kit• Purify DNA without column-clogging lysate filtration steps• Material-specific protocols provided

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Yields of high–copy plasmid DNA obtained using GeneJET Plasmid Midiprep Kit and other kits for midscale plasmid DNA purification at maximal recommended bacterial cell culture volumes.



Promo Code Product Preps

K0482-MP33K0481-MP33

GeneJET Plasmid Midiprep Kit10025

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K0782-MP33K0781-MP33

GeneJET Whole Blood Genomic DNA Purification Mini Kit

25050

K0792-MP33K0791-MP33

GeneJET Plant Genomic DNA Purification Kit

25050

GeneJET™ Whole Blood RNA Purification Mini Kit• Purify DNA-free RNA with no DNase step• High RNA integrity (RIN > 8.0)GeneJET™ Plant RNA Purification Kit• Purify DNA-free RNA with no DNase step• High RNA integrity (RIN > 8.0)

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High purity total RNA isolation

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Spin columns based on silica-membrane technology. Specialized and detailed protocols for different sample types.Purified RNA can be used in a wide range of downstream applications.

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Superior transfection efficiency with minimal toxicity Thermo Scientific TurboFect Transfection Reagent is the reagent of choice for shRNA and plasmid transfection applications.

• High transfection efficiency of a wide variety of cell types, including primary, differentiated and undifferentiated cells

• Transfection can be performed in the presence or absence of serum• Demonstrates superior transfection efficiency and minimal toxicity when compared to lipid-based or

other polymer-based transfection reagents• Simple 3-step transfection protocol compared with 6-7 steps for other transfection reagents

Effective April 1, 2012, TurboFect™ Transfection Reagent will replace ExGen 500, Arrest-In™ and Express-In Transfection Reagents.

Orders for these discontinued products will continue to be processed until March 31, 2012.

If you require further information, please contact Technical Support at [email protected].

Thermo Scientific TurboFect Transfection Reagent demonstrates higher transfection efficiency and improved plasmid expression across cell lines. Cells were transfected with plasmid DNA encoding eGFP with the indicated transfection reagent (NTC; non-treated control). Transfections were performed according to manufacturers’ recommendations and subsequent GFP expression was analyzed by flow cytometry.

Transfection efficiencyMean fluorescence intensity

GFP

Posi

tive

Cells

(%)

Mea

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ce In

tens

ity

NTC TurboFect Lipofect- Fugene 6 amine 2000

100

75

50

25

0

2000

1750

1500

1250

1000

750

500

250

0

GFP

Posi

tive

Cells

(%)

Mea

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ce In

tens

ity


50

40

30

20

10

0

1500

1250

1000

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250

0

WEHI CellsHeLa Cells HepG2 Cells


GFP

Posi

tive

Cells

(%)

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60

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K0761-MP33GeneJET Whole Blood RNA Purification Mini Kit

50

K0802-MP33K0801-MP33

GeneJET Plant RNA Purification Kit25050


R0531-MP33 TurboFect Transfection Reagent 1,0 mL

Thermo Scientific PCR and qPCR

Thermo Scientific Direct PCR KitsDirect PCR saves you time and money by allowing amplification of DNA directly from the sample material. No DNA purification is needed. The Direct PCR approach is based on unique Thermo Scientific Phusion and Phire DNA Polymerases that are extremely tolerant of PCR inhibitors present in sample materials and guarantee high yields of specific PCR product.• No need for time-consuming and expensive DNA purification steps• Simple and short protocols with minimal hands-on time• Very little starting sample material required• Control primers and detailed instructions included in each kit• Optimized kits for plant, animal tissues, blood and human specimen

Find out more information about Direct PCR at www.thermoscientific.com/directpcr

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Take a more direct route to PCR

Customer feedback on Phire Animal Tissue Direct PCR Kit:

The quality of PCR results was as good or better than with more time-consuming extraction methods, followed by PCR (in terms of yield and clarity of bands on the agarose gel), so it allowed us to streamline the species ID assay. Given the small amount of tissue required, it will also allow us to refine our tissue collection methods so that we can take minute fin samples from live fish.

- Dr. Margaret Docker, Department of Biological Sciences, University of Manitoba, Canada

I used the Phire Animal Tissue Direct PCR Kit on the nematode C.elegans. I achieved good clean product with results comparable to using extracted DNA. By side stepping the need to extract and clean up the DNA we can save up to half a day using the Direct PCR kit, great when we are working with a number of different strains.

- Laura Weldon, Technician, Nematode Biology Research, Bristol University, UK



F-130-MP33 Phire Plant Direct PCR Kit 200 x 50 µl rxns

F-140-MP33 Phire Animal Tissue Direct PCR Kit 200 x 50 µl rxns

F-150-MP33 Phusion Human Specimen Direct PCR Kit 200 x 20 µl rxns

F-547S-MP33 Phusion Blood Direct PCR Kit 100 x 20 µl rxns

F-547L-MP33 Phusion Blood Direct PCR Kit 500 x 20 µl rxns

G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G

© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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Order your free Thermo Scientific PCR and qPCR Product Guide

qPCR reagents and first strand cDNA synthesis kits for fast real-time qPCR

Request a free copy from your local Fisher office

Benefits• State-of-the-art products from the Thermo Scientific

ABgene, Finnzymes and Fermentas product lines• Contains a complete workflow of PCR and qPCR products,

including reagents, instruments and plastic consumables• Features product selection tables to assist in finding the

best solution for your application

Standard qPCR instrument

Time savings using fast qPCR reagents

Fast qPCR instrument

120

100

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60

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Reagent for standard cyclingFast qPCR reagent

Min

utes

Thermo Scientific DyNAmo Flash and ColorFlash qPCR kits for SYBR Green and probe chemistries – optimized 2x master mixes for extremely fast qPCR protocols.• The engineered Tbr DNA polymerase allows a combined annealing and extension

step of only 15 seconds• Specific and sensitive detection• Included dUTP allows the use of UNG for prevention of carry-over contamination• Blue master mix combined with yellow sample buffer turns the reaction mix green

that helps ensure correct pipetting (DyNAmo ColorFlash Kits)

Thermo Scientific Maxima First Strand Synthesis Kits utilizes Maxima Reverse Transcriptase, an advanced enzyme derived from in vitro evolution of M-MuLV RT that delivers faster cDNA synthesis than ever before.• Increased synthesis rates – complete cDNA synthesis in 15 minutes• Sensitive and reproducible – cDNA synthesis from a wide range of total RNA

amounts (1 pg – 5 μg)• Increased reaction temperatures - active up to 65°C• Increased reproducibility – reduced bias



F-416L-MP33 DyNAmo ColorFlash SYBR Green qPCR Kit 500 x 20 μL rxns

F-415L-MP33 DyNAmo Flash SYBR Green qPCR Kit 500 x 20 μL rxns

F-456L-MP33 DyNAmo ColorFlash Probe qPCR Kit 500 x 20 μL rxns

F-455L-MP33 DyNAmo Flash Probe qPCR Kit 500 x 20 μL rxns

K1641-MP33K1642-MP33

Maxima First Strand cDNA Synthesis Kit for RT-qPCR (pre-mixed kit components)

50 rxns200 rxns

Ordering Information

Belgique / België tél 056 260 260 fax 056 260 270 [email protected] www.be.fishersci.comEspaña tel 902 239 303 fax 902 239 404 [email protected] www.es.fishersci.comFrance tél 03 88 67 53 20 fax 03 88 67 11 68 [email protected] www.fr.fishersci.comItalia tel 02 953 28 258 fax 02 953 27 374 [email protected] www.it.fishersci.comNetherlands tel 020 487 70 00 fax 020 487 70 70 [email protected] www.nl.fishersci.comPortugal tel 21 425 33 50/4 fax 21 425 33 51 [email protected] www.pt.fishersci.comSuisse/Switzerland tél 056 618 41 11 fax 056 618 41 41 [email protected] www.ch.fishersci.com

Lit code PB_2012_13_WEUR

marketplace winter2012 q1 weur final

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