masaryk university enantis, s.r.o. vop-026 Šternberk, s.p., division vtÚo brno cpn, s.r.o. e n z y...
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Masaryk University
Enantis, s.r.o.
VOP-026 Šternberk, s.p., division VTÚO Brno
CPN, s.r.o.
E N Z Y M A T I C D E C O N T A M I N A T I O N
O F S U L F U R M U S T A R D
W a r f a r e a g e n t s u l f u r m u s t a r d
used for the first time in 1917 near Belgium city Ypres
easily penetrates to various materials
makes burn marks and blisters due to skin contacts
military use banned by international convention
easy to prepare from common chemicals
T e c h n o l o g y d e v e l o p m e n t
Czech Republic – development of enzymatic decontamination mixture
for sulfur mustard prioritized by NATO
research projects funded by Ministry of Defence 2004 – 2006 and
Ministry of Education 2005 – 2011
degradation of sulfur mustard by enzymes dehalogenases demonstrated
protection of intellectual properties – Czech patent CZ 298287
technology transfer to practice – biotechnology spin-off Enantis
H a l o a l k a n e d e h a l o g e n a s e s
H a l o a l k a n e d e h a l o g e n a s e s
T o x i c s u l f u r m u s t a r d
S
Cl
Cl
ClO
Cll
N o n t o x i c a n a l o g u e
C o m p u t e r m o d e l l i n g
C o m p u t e r m o d e l l i n g
C o m p u t e r m o d e l l i n g
bis(2-chloroethyl) ethersulfur mustard
C o m p u t e r m o d e l l i n g
E x p e r i m e n t a l t e s t s
0
0.2
0.4
0.6
0.8
1
1.2
1.4
0 5 10 15 20
Time (min)
Su
lfu
r m
ust
ard
co
nc.
(m
M)
Spontaneous degradation
Enzyme catalysed hydrolysis
Exponenciální (Spontaneousdegradation)Exponenciální (Enzymecatalysed hydrolysis)
E x p e r i m e n t a l t e s t s
0
0.2
0.4
0.6
0.8
1
1.2
1.4
0 5 10 15 20
Time (min)
Su
lfu
r m
ust
ard
co
nc.
(m
M)
Spontaneous degradation
Enzyme catalysed hydrolysis
Exponenciální (Spontaneousdegradation)Exponenciální (Enzymecatalysed hydrolysis)
P a t e n t - p r o t e c t e d c o n c e p t
R e p o r t s i n s c i e n t i f i c j o u r n a l s
T e c h n o l o g y t r a n s f e r t o p r a c t i s e
1. development of decontamination mixture
2. optimisation of protein production
3. large-scale industrial production
4. product commercialization
5. development of new and improved products
P r o d u c t d e v e l o p m e n t a n d t e s t i n g
testing of dehalogenases in decontamination aerosols
(Pacific Northwest National Laboratory – USA)
testing of dehalogenases in polymer particles
(Foster & Miller, US Army – USA)
testing of dehalogenases in microemulsions
(Defence Science and Technology Laboratory – Great Britain)
testing of dehalogenases in skin decontamination cream
(Bundeswehr Institute of toxicology and pharmacy – Germany)
NATO testing of enzyme decontamination on aircraft interriers
C o n s o r t i u m o f p a r t n e r s
Masaryk University and Enantis, s.r.o.
14 years of research on dehalogenases
> 40 genes, >100 protein variants
know-how from 29 research stages
modern laboratories and facilities for protein engineering
E N Z Y M E D I S C O V E R Y A N D O P T I M I Z A T I O N
isolation and construction of new enzymes
genetic breeding of enzymes with improved catalytic properties
C o n s o r t i u m o f p a r t n e r s
Masaryk University and Enantis, s.r.o.
VOP-026 Šternberk, s.p., division VTÚO Brno
applied research and development of decontaminations and defence against warfare
of mass destruction
personal and infrastruture based on 50 years of experience
T E S T I N G O F E N Z Y M E S W I T H W A R F A R E A G E N T S
study of warfare chemicals with extreme toxic properties
compatibility testing of individual components for decontamination mixtures
C o n s o r t i u m o f p a r t n e r s
Masaryk University and Enantis, s.r.o.
VOP-026 Šternberk, s.p., division VTÚO Brno
CPN, s.r.o.
Production of pharmaceuticals and cosmetics
large-scale industrial fermentations and down-stream processing
P R O C E S S S C A L E – U P A N D P R O D U C T I O N
optimization of enzyme production, pilot-scale and full production
development of protective cream for skin decontamination
E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r
high-quality basic research:
systematic and selective financial support
investment into human resources
competitive infrastructure
financial support and consulting for protection of intellectual properties
business consulting and administrative support
collaboration with local industrial partners
E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r
high-quality basic research:
systematic and selective financial support
investment into human resources
competitive infrastructure
financial support and consulting for protection of intellectual properties
business consulting and administrative support
collaboration with local industrial partners
E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r
high-quality basic research:
systematic and selective financial support
investment into human resources
competitive infrastructure
financial support and consulting for protection of intellectual properties
business consulting and administrative support
collaboration with local industrial partners
A c k n o w l e d g e m e n t s
CTT
Jan Josef Loschmidt
(1821 – 1895)
E x c u r s i o n t o l a b o r a t o r i e s
established 1994, funded by industry, 1 laboratory
until today 18 grants, 6 international
laboratories 5, instrumentation 1 mil EUR
in parallel 12 research projects
know-how from 29 research stages, 11 visitors
courses Bioinformatics and Biocatalysis
E x c u r s i o n t o l a b o r a t o r i e s
E x c u r s i o n t o l a b o r a t o r i e s
3 doctors, 10 doctoral students, 5 diploma students, 1 technician