master‘s thesis topics human biology physiology · master‘s thesis topics human biology ....
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Master‘s Thesis Topics Human Biology
Physiology
Topic Molecular mechanisms involved in the homeostasis of phosphate
Short description
The maintenance of the extracellular concentration of inorganic phosphate (Pi) is crucial for a variety of cellular processes, bone formation and the prevention of vascular calcification. Projects related to this topic will focuss on molecular aspects (cellular regulations, interacting proteins, structure-function relationships) of different sodium-dependent phosphate cotransporters. Also, whole animal studies aim to understand the role of the different Na/Pi-cotransporters (in different organs) in the physiology and pathophysiology of inorganic phosphate metabolism.
Supervisor Institute E-mail Phone
Dr. Nati Hernando, Dr. Ian Forster Institute of Physiology-University Zürich-Irchel [email protected], [email protected] +41 44 635 5032
Conditions Some projects will include animal experiments
Links www.uzh.ch/physiol
Topic Sirtuins in atherosclerosis
Short description
Atherosclerosis is a chronic inflammatory disease of the vascular system in our aging population. Endothelial activation and subsequent infiltration of the arterial intima promote plaque formation. Rupture of these plaques leads to myocardial infarction and stroke. Sirtuins are a family of NAD-dependent deacetylases that regulate cellular function through deacetylation of a wide range of protein targets. Many sirtuins are critically involved in regulating metabolism and cellular fate in age-related diseases, such as atherosclerosis. The study of sirtuins in atherosclerosis is one of the focuses of our group and provides frequent opportunities for master projects. Please inquire.
Supervisor Institute E-mail Phone
Dr. Chrisitian Matter Center for Molecular Cardiology, UZH, Schlieren Campus [email protected] +41 44 635 64 67
Conditions None
Links http://www.kardiologie.usz.ch/LehreUndForschung/Grundlagenforschung/ Seiten/Atherosclerosisandmetabolicdisease.aspx
Topic Vascular Dysfunction in Aging & Disease
Short description
Vascular homeostasis is critical for the correct supply of nutrients and oxygen to all organs. The endothelium -the innermost layer of a vessel- functions as an active barrier to allow the passage of different substances; additionally, it mediates vascular dilatation and constriction.In disease states the endothelium becomes dysfuctional and through complex cascades of events leads to several complications such as atherothrombosis. Several key factors such as free radicals are implicated in endothelial dysfnction observed in disease states. Our group performs research aimed at elucidating the interaction of regulators and mediators of vascular function in disease states and aging.
Supervisor Institute E-mail Phone
Dr. Giovanni G. Camici Center for Molecular Cardiology, UZH, Schlieren Campus [email protected] +41 44 635 64 68
Conditions None
Links http://www.physiol.uzh.ch/research/CardiovascularResearch.html
Topic MSc Projects in Cellular Oxygen Physiology
Short description
The ability of cells to sense and respond to reduced oxygen conditions (hypoxia) is crucial in many physiological and pathophysiological processes. The micro-environment of many tumor types is hypoxic because rapidly proliferating tumor cells consume more oxygen than can be supplied. Hypoxia leads to activation of the hypoxia-inducible transcription factor (HIF). In normoxia is the HIF alpha subunit hydroxylated by HIF prolyl hydroxylases (PHDs) and rapidly degraded. Our projects aim to understand how oxygen-sensing protein hydroxylases are regulated and to identify novel hydroxylation targets.
Supervisor Institute E-mail Phone
Prof. Dr. Roland H. Wenger / Dr. Carsten Scholz Institute of Physiology [email protected] / [email protected] +41 44 635-5065 / +41 44 635-5075
Conditions Good knowledge of biochemistry, molecular and cell biology
Links http://www.physiol.uzh.ch/research/CellularOxygenPhysiology_en.html
Topic Human milk oligosaccharides and intestinal bacteria
Short description
Human milk contains a large group of complex oligosaccharides, which influence the bacterial colonization of the newborn intestine. Recently, specific human milk oligosaccharides have also been shown to regulate immune cell functions. Using pure milk oligosaccharides, our group investigates the role of these compounds on intestinal bacterial composition (in vitro and in vivo) and on the maturation of mucosal immune cells (in cell culture and mouse models). Methods applied are leukocyte isolation, cell culture, flow cytometry, real-time PCR, gene expression and inactivation in bacteria.
Supervisor Institute E-mail Phone
Prof. Dr. Thierry Hennet Institute of Physiology [email protected] +41 44 635 5080
Conditions Interest in genetics and pathogenesis of diseases
Links http://www.uzh.ch/physiol/
Topic Mechanismen und Regulation der normalen und kranken Niere
Short description
Die Nieren spielen eine zentrale Rolle in der Regulation der Zusammensetzung vieler Körperflüssigkeiten und beeinflussen direkt und indirekt die Funktion von Knochen, Muskel, Metabolismus, Blutdruck, Säuren-Basengleichgewicht etc. Verschiedene Masterarbeiten werden angeboten, die im Tier- und/oder Zellmodell Transportfunktionen der Niere, ihre Regulation und Adaptation und ihre Dysregulation in verschiedenen Nierenerkrankungen untersuchen. Methodisch verwenden wir Standardtechniken der Protein- und Molekularbiologie, Tierversuche, Zellkultur, Arbeit an Geweben, Immunhistochemie, intrazelluläre Kalzium/pH/ Ionenmessungen mittels Fluoreszenz in intakten Geweben/Zellen, etc.
Supervisor Institute E-mail Phone
Prof. Dr. Carsten Wagner Institute of Physiology [email protected] +41 44-63 55023
Conditions keine, Besuch des LTK Moduls1 (Einführung in Tierversuche) für manche Projekte nützlich, aber nicht zwingend
Links http://www.physiol.uzh.ch/research/Acidbasetransport.html
Topic Biology of amino acid transport processes
Short description (max. 10 Zeilen)
• The general aim of our research is to understand the control of amino acid transport from the level of individual transporters up to its integration in the organism. The research focuses in particular on the cooperation of amino acid transporters within renal and intestinal epithelia and blood brain barrier endothelium. Understanding the mechanisms of transepithelial and transendothelial amino acid transport could help devise strategies for treating genetic and acquired amino acid transport disorders and for influencing drug (re)absorption. • Main experimental models: cultures of (epithelial) cells, heterologous expression of gene products, gene-modified mice • Techniques: transport assays, gene expression studies, immuno-biochemistry, immuno-localization, gene arrays, (life) imaging, metabolic studies in mice, electrophysiology etc.
Supervisor Institute E-mail Phone
Prof. Dr. François Verrey, Dr. Simone Camargo Institute of Physiology [email protected] +41 44 635 5044/46
Conditions Gesucht werden KandidatInnen die in einem jungen, dynamischen Team und einer interdisziplinären Umgebung arbeiten wollen
Links http://www.physiol.uzh.ch/research/EpithelialTransports_en.html
Topic Functional mouse genetic approaches to model human tumours and identify personalized molecular targets for cancer therapy.
Short description
Our laboratory seeks to understand the molecular and cellular basis of kidney and uterine cancers using advanced mouse genetic approaches. We have developed a powerful lentiviral-based gene regulation system that allows the simultaneous alteration of the expression of multiple genes. We undertake a reverse engineering approach to attempt to “build” tumours by inducing in primary cells and in mouse tissues the combinatorial genetic changes that are believed to cause tumours in humans. Additionally we employ high-throughput shRNA genetic screening to identify molecular therapeutic targets that are specific for the combinations of genetic alterations that cause each individual tumour.
Supervisor Institute E-mail Phone
Prof. Dr. Ian Frew (together with postdoctoral scientists) Institute of Physiology [email protected] +41 44 635 5004
Conditions 12 month Master’s thesis preferred, enthusiasm for experimental cancer research, strong academic background in cellular and molecular biology
Links http://www.physiol.uzh.ch/research/GrFrew.html
Topic Obesity, eating control and metabolic diseases
Short description
Obesity and type 2 diabetes are worldwide health epidemics that dramatically increase metabolic and cardiovascular diseases. The control of food intake and body weight involves numerous hormones released from the gastrointestinal tract. Some of these hormones, like the pancreatic peptide amylin or GLP-1, contribute to the control of meal ending satiation. Other hormones, like leptin, reflect the amount of body fat stores. Our research focuses on the central neural pathways mediating amylin’s anorectic action as well as on the interaction of amylin with other hormones. In addition, we study various aspects of the role of bariatric surgery (Roux-en-Y gastric bypass) in improving metabolism. Experimental techniques include behavioral feeding studies, immunocytochemistry, electrophysiology and functional tests of the reward system. Further, by using indirect calorimetry, we can assess energy intake and energy expenditure simultaneously. We are also interested in dissecting the molecular pathways of vascular disease in the development of type 2 diabetes, and the improvement of cardiovascular health after gastric bypass surgery. We focus on the following collaborative projects between the Center for Molecular Cardiology at UZH, the Department of Visceral Surgery at USZ, and the Institute of Veterinary Physiology: - The role of pancreatic amylin on the development of vascular dysfunction in a transgenic rat model of type 2 diabetes; - The role of gastric bypass surgery and JNK inhibition on the improvement of vascular dysfunction and HDL metabolism and a rat model of non-diabetic diet-induced obesity; - The role of gastric bypass surgery and liraglutide treatment on the improvement of HDL metabolism in human obese patients.
Supervisor Institute E-mail Phone
Prof. Dr. Thomas A. Lutz Institute of Veterinary Physiology [email protected] +41 44 - 635 8808
Conditions Master students willing to participate in one or more of these projects will learn the following skills and methods: animal handling (rat) including in vivo tests of glucose and insulin sensitivity and animal necropsy; in vitro dissecting of molecular pathways by western blot, primary human endothelial cell culture, blood HDL isolation and characterization, various enzymatic and ELISA assays.
Links www.vetphys.uzh.ch
Topic The Effect of Erythropoietin (Epo) on Cancer Growth
Short description
Erythropoietin (Epo) is an approved substance to treat cancer-associated anemia. However, several clinical and preclinical studies suggest that Epo might promote tumor growth. In this master thesis we will screen human cancer cell lines for Epo receptor (EpoR) expression with our newly developed antibody. EpoR positive cell lines will be tested in vitro to analyze their response to Epo treatment cell biologically. In addition we will perform in vivo experiments with immunodeficient mice to analyze the impact of Epo on cancer.
Supervisor Institute E-mail Phone
Prof. Dr. Max Gassmann Institute of Veterinary Physiology [email protected] +41 44 635 8801
Conditions We are looking for biology, medical or veterinary students.
Links www.vetphys.uzh.ch
Topic Neuronal and vascular responses to reduced oxygenation : physiology and pathology
Short description
Efficient oxygen delivery to brain tissues is crucial for neuronal function and thus proper brain function. Hypoxia characterises a variety of physiological events but also contributes significantly to progression of pathogenesis and widespread diseases including, cancer, stroke and neurodegenerative disorders. When oxygen is limited cells activate key adaptive responses mediated largely by hypoxia-inducible factor-1 (HIF-1). We recently found that the multifunctional Rho GTPases are involved in the neuronal hypoxic response and likely modulate HIF stabilisation. Our group performs research investigating the molecular mechanisms of neuronal and vascular adaptation to oxygen deprivation with emphasis on the role of RhoGTPases in regulation of HIF-1 in primary neurons and other brain specific cells in disease states and during aging.
Supervisor Institute E-mail Phone
Dr. Lara Ogunshola Institute of Veterinary Physiology [email protected] +41 44 635 8805
Conditions Basic knowledge in molecular biology and/or medical physiology
Links www.vetphys.uzh.ch
Anatomy Topic Kontrolle der Salzausscheidung und des Blutdruckes durch die Nieren
Short description
Die Nieren regulieren den Salzhaushalt und spielen eine wichtige Rolle bei der Kontrolle des Blutdruckes. Wir untersuchen die zugrundeliegenden zellulären und molekularen Mechanismen unter Nutzung einer Vielzahl von experimentellen Systemen (Mausmodell, Zebrafisch, Zellkultur, heterologe Expressionssysteme). Zur Anwendung kommen diverse Methoden zur Phänotypisierung der Mausmodelle inkl. Urin- und Plasma-analysen, Blutdruck-Messungen, qRT-PCR, Licht- und Elektronenmikroskopie, Ionentransportstudien, Immunoblots, Immunohistochemie, etc.
Supervisor Institute E-mail Phone
Prof. Dr. Johannes Loffing Anatomisches Institut [email protected] +41 44 635 5320
Conditions
Links http://www.anatom.uzh.ch/research/Divisionloffing_en.html
Endocrinology and Diabetes
Topic Insulin receptor substrates and the development of type 2 diabetes
Short description
We study the function of insulin receptor substrates in the context of the regulation of blood glucose homeostasis. IRS1 and IRS2 are required in the periphery for insulin action and in the pancreas for β-cell function. Candidates can choose between several topics such as control of β-cell mass and function, insulin resistance and molecular mechanisms of signal transduction in general.
Supervisor Institute E-mail Phone
Dr. Markus Niessen UniversitätsSpital, Endokrinologie und Diabetologie, Rämistr. 100 [email protected] 044-255 22 25
Conditions
Links http://www.research-projects.uzh.ch/p6959.htm; http://www.research-projects.uzh.ch/p6960.htm; http://www.med.uzh.ch/ZKF/Website-1/ResearchGroups/AlphabeticOrder/Niessen.html
Experimental Ophthalmology
Topic Molecular Mechanisms of Retinal Degeneration
Short description
Many blinding diseases are caused by the degeneration of photoreceptor cells. Using several animal models of induced and inherited retinal degeneration, our research aims at the understanding of the molecular mechanisms and signaling pathways induced during the degenerative process. The acquired knowledge of the cell death pathways is used to develop and test neuroprotective strategies to improve cell viability and to rescue vision. Available projects are dealing with different aspects of molecular mechanisms during retinal degeneration and neuroprotection.
Supervisor Institute E-mail Phone
Prof. Dr. Christian Grimm Lab for Retinal Cell Biology Dept Ophthalmology, University Hospital Zurich [email protected] 044 255 3905
Conditions Interest in various aspects of the visual system. Background in molecular biology desirable. Good knowledge of the english language is an advantage.
Links http://www.research-projects.uzh.ch/a1009.htm
Medical Molecular Genetics
Topic Genetic Basis of Eye Diseases
Short description
A Master / Diploma student position is available to identify new genes involved in the pathogenesis of exudative vitreoretinopathy (EVR) and to investigate their function in order to better understand the molecular basis of this group of eye diseases. In our lab, we focus on the investigation of the molecular pathophysiology of the disease phenotype, which is quite similar in this group of vasoproliferative blindness diseases, especially with regard to the defects in the retinal vasculature. More information is also available on our website: http://www.medmolgen.uzh.ch/research/eyediseases/norriedisease.html We found several, probably disease-causing, DNA sequence alterations in different genes of patients with EVR which are now being characterized in more detail. The project involves functional analysis of mutations in vitro and sequencing of new candidate genes for EVR. Duration: Nine month to one year. Entrance upon: As soon as possible.
Supervisor Institute E-mail Phone
Prof. Dr. Wolfgang Berger Institute of Medical Molecular Genetics [email protected] 044 655 70 34
Conditions Motivated students in the area of biology, biomedical sciences or equivalent are encouraged to send applications. Interest in human genetics as well as basic training or practical experience in molecular genetics and molecular biology is an advantage.
Links www.medmolgen.uzh.ch
Topic Membrane Transporter SLC16A12 (MCT12) and Cataract
Short description
The avascular lens in the eye requires special membrane transporters that allow the supply of metabolites and the removal of waste products to and from the central part of the lens. If this process is disturbed, opacities may result, which will cause cataracts and impaired vision. The project involves screening for additional mutations by DNA sequencing of patient DNA. Function will be studied by constructing the mutations and testing their expression and function in cell culture using RT-PCR, luciferase reporter assay, statistics, immunohistochemistry, Western blots, bioinformatics.
Supervisor Institute E-mail Phone
Dr. Barbara Kloeckener-Gruissem Institute of Medical Molecular Genetics [email protected] 044 655 70 34
Conditions Basic knowledge of molecular genetics and techniques in molecular biology, being curious and motivated.
Links www.medmolgen.uzh.ch
Cancer
Topic Inflammatory microenvironment and metastasis
Short description
Metastasis is the primary cause of cancer-related mortality. Tumor microenvironment is composed of leukocytes and stromal cells that significantly affect cancer progression. Chemokines are the key cytokines, which promote the recruitment and the polarization of leukocytes. Our projects aim to understand the function of particular chemokine-chemokine receptors pairs using in vitro techniques (e.g. co-culture assays) and validate their role in vivo.
Supervisor Institute E-mail Phone
PD Dr. Lubor Borsig Institute of Physiology [email protected] 044 635 5134
Conditions The project may include animal models
Links http://www.physiol.uzh.ch/research/institutegroups/grborsig.html
Topic Identifikation und Charakterisierung von Krebsstammzellen in pädiatrischen Sarkomen
Short description
Am Modell des Rhabdomyosarkoms werden in diesem Projekt erstmals bei einem pädiatrischen Sarkom Krebsstammzellen isoliert, auf der Basis der funktionellen Bildung von Zell-"Spheres". Diese werden ausgehend von Krebszellen sowie von adulten myogenen Stammzellen gebildet und eingehend vergleichend charakterisiert. Dies umfasst u.a. das Differenzierungspotential, klonogene Wachstumseigenschaften, sowie mögliche Stammzell-Unterpopulationen. Im weiteren sollen die "Spheres" mit ausgewählten zielgerichteten Medikamenten behandelt, und Proliferation, Zelltod sowie Transkriptome vergleichend gemessen werden.
Supervisor Institute E-mail Phone
Prof. Dr. Beat W. Schäfer Universitäts-Kinderklinik, Onkologie [email protected] 044 2667553 oder 6348852
Conditions
Links www.kispi.uzh.ch/onkologie
Topic Strahlenresistenz auf molekularer und zellulärerEbene
Short description
Schädigung der DNA ist die wichtigste Ursache für den strahleninduzierten Zelltod. Während den letzten Jahren zeigte die moderne Krebsforschung jedoch, dass ionisierende Strahlung auch Signalübermittlungskaskaden unabhängig von der DNA-Schädigung in der Zelle auslöst, welche das Therapieansprechen und die Therapieresistenz massiv mitbestimmen. Das Ziel unserer Projekte ist a) die molekulare und zellbiologische Untersuchung solcher strahleninduzierten Signalüber-mittlungskaskaden in genetisch-kontrollierten und klinisch-relevanten Tumormodellen, und b) die Entwicklung von Kombinationstherapie-modalitäten mit klinisch-relevanten Substanzen, die zur Ueberwindung der Strahlenresistenz führen.
Supervisor Institute E-mail Phone
Prof. Dr. Martin Pruschy Labor Molekulare Radiobiologie, Universitätsspital Zürich [email protected] 044 255 8549
Conditions
Links http://www.cnz.uzh.ch/pruschy.html
Topic Hermatologic malignancies
Short description
Different MSc projects are available in the leukemia research group at the University Children's Hospital Zurich. Projects will deal with different aspects of drug resistance in childhood acute leukemia. One project will address the role of an alternative cell death mechanism, necroptosis, in the resensitization of resistant leukemia cells to steroids. We have furthermore developed a platform to analyze the antileukemic potential of new agents in primary leukemia cells from highly drug resistant patients. This project will deal with the analysis and characterization of the hereby identified new antileukemic agents also with respect to their activity in combination with current chemotherapy.
Supervisor Institute E-mail Phone
PD Dr. Jean-Pierre Bourquin / Dr. Beat Bornhauser Labor Molekulare Dept of Oncology, University Children's Hospital [email protected] / [email protected] 044 266 7304; 044 634 88 17
Conditions Commitment and motivation
Links http://idcmskispi.unizh.ch/lenya/kispi/live/af/ForschungLehre/onkoonco/ Leukaemie_de.html
Topic Detection of BRAF mutations in non-small cell lung carcinoma (NSCLC) using microfluidic probe (MFP) based DNA extraction
Short description
BRAF mutations are found in a subset of non-small cell lung carcinoma. The mutation is predictive for response to targeted BRAF inhibition. Tumor initiation by oncogenic BRAF renders cancer cells susceptible to TGFβ- induced epithelial-mesenchymal transition (EMT). In order to assess the BRAF mutation status in both tumor epithelia and surrounding stroma of formalin-fixed and paraffin-embedded NSCLC tumors and to correlate it with the expression of EMT-related proteins we will use the microfluidic probe (MFP) technology, developed by IBM Rüschlikon.
Supervisor Institute E-mail Phone
PD Dr. Peter Schraml Institute of Surgical Pathology [email protected] 044 255 2114
Conditions Interest in cancer research and biomedical engineering. Motivated, self-driven and keen to work on experiments. Master thesis:12 months
Links http://www.klinische-pathologie.usz.ch/
Clinical Chemistry
Topic Anti-Inflammatory Pathways in Atherosclerosis
Short description
Atherosclerosis is the pathogenetic process leading to myocardial infarction and stroke and still is the leading cause of death in Western civilisation. Chronic inflammation plays a major role in atherogenesis and lack of resolution of inflammation aggravates the vicious circle of inflammation in atherogenesis. Functional polymorphisms in genes involved in the resolution of inflammation will eventually alter the balance between pro- and anti-inflammation. We screen these genes for polymorphisms in the Caucasian population, investigate the functionality of such newly discovered polymorphisms in cell culture and investigate their association with atherosclerosis in patients with coronary artery disease.
Supervisor Institute E-mail Phone
Prof. Dr. Martin Hersberger Division of Clinical Chemistry and Biochemistry University Children's Hospital Zürich Steinwiesstrasse 75 CH-8032 Zürich [email protected] 044 / 266 75 41
Conditions Experience with cell cultures is an advantage
Links http://www.kispi.uzh.ch/af/ForschungLehre/ClinicalChem_de.html
Topic The Role of Lipid Mediators in Chronic Inflammatory Diseases
Short description
We are interested in the regulation and in the effect of the resolution of inflammation on chronic inflammatory diseases, like atherosclerosis and asthma with the aim to find pathogenetic mechanisms, new genetic factors for disease risk prediction, and novel therapeutic approaches. For this, we investigate the genetic regulation and the molecular pathways of enzymes and receptors, which are involved in lipid mediator synthesis and in their signal transduction.
Supervisor Institute E-mail Phone
Prof. Dr. Martin Hersberger Division of Clinical Chemistry and Biochemistry University Children's Hospital Zürich Steinwiesstrasse 75 CH-8032 Zürich [email protected] 044 / 266 7541
Conditions Experience in mass spectrometry is an advantage
Links http://www.kispi.uzh.ch/af/ForschungLehre/ClinicalChem_de.html
Topic Intracellular transport of HDL in endothelial cells
Short description
Plasma levels of HDL are inversely correlated with the risk of cardiovascular diseases and exert potentially anti-atherogenic properties within the arterial wall. However, it is unknown how they are transported from the blood stream through the endothelium into the vascular wall. Therefore we investigate the interaction of HDL with this barrier. The aim of the study is to characterize the interaction of native and reconstituted HDL with cultivated endothelial cells. In a second step we will use RNA interfence to identify the receptors and transporters which are needed to mediate endothelial transcytosis of HDL particles.
Supervisor Institute E-mail Phone
Dr. Lucia Rohrer / Prof. Dr. A. von Eckardstein Institut für Klinische Chemie, Universitätsspital [email protected] 044 255 4793
Conditions
Links
Topic Serine-Palmitoyltransferase and Sphingolipid Metabolism
Short description
Sphingolipids and their metabolites are ubiquitous constituents of cell membranes and involved in various cellular functions like apoptosis, signal transduction and membrane trafficking. The serine-palmitoyltransferase (SPT) is the key regulatory enzyme in the sphingolipid synthesis pathway. Mutations in the SPT gene result in an inherited sensory neuropathy (HSN1). Pathological changes in sphingolipid metabolism have been implied to play pathogenetic roles in various diseases including Diabetes Type 2, atherosclerosis and cancer. We previously identified and characterized a third subunit of SPT and offer several MSc projects to further characterize the structure, function and regulation of the subunits of SPT.
Supervisor Institute E-mail Phone
Dr. Thorsten Hornemann Inst. für Klinische Chemie (IKC), Unispital Zürich [email protected] 0041 1 255 4719
Conditions Experiences in cell culture, protein chemistry and molecular biology are preferable
Links
Topic Identification of protein biomarkers for atherosclerosis
Short description
Ruptures of atherosclerotic plaques in coronary arteries lead to acute coronary syndromes (heart attack). We are looking for proteins that leak from atherosclerotic lesions into the blood stream, which can be used as diagnostic markers. We use antibody phage display to generate antibodies against proteins from atherosclerotic plaques and antibodies against certain posttranslational modifications, which are believed to occur in atherosclerotic plaques and leak into the blood stream. These antibodies are used to detect the proteins in plasma samples from patients, and to purify these proteins so that they can be identified by mass spectrometry.
Supervisor Institute E-mail Phone
Dr. Danielle Hof / Prof. Dr. Arnold von Eckardstein University Hospital Zurich, Institute for Clinical Chemistry [email protected] 044 255 30 24
Conditions A background and interest in molecular biology, biochemistry, or biotechnology.
Links www.ikc.usz.ch
Metabolic Medicine
Topic Neurodegeneration due to mutant methylmalonyl-CoA mutase
Short description
Methylmalonic aciduria is a rare inborn error of metabolism that predominantly presents in infancy with neurological symptoms including acute metabolic and encephalopathic crises. It is caused by deficiency of the methylmalonyl-CoA mutase (MCM) which plays a key role in the catabolism of various amino acids, as well as of odd-chain fatty acids and the side chain of cholesterol. MCM binds cobalamin to form the active holoenzyme, and defects of MCM can be subdivided into responder and non-responder to cobalamin. The aim of this thesis is to express human and murine MCM in vitro as a prerequisite to validate a mutant MCM that will be used to generate a transgenic knock-in mouse as a new model for methylmalonic aciduria.
Supervisor Institute E-mail Phone
Prof. Dr. M. Baumgartner, Prof. Dr. Beat Thöny Universitäts-Kinderkliniken Zürich, Abteilungen Stoffwechsel und Klinische Chemie und Biochemie [email protected] 044-266 76 22
Conditions Keine
Links www.kispi.uzh.ch/Kinderspital/Medizin/Medizin/Stoffwechsel_de.html; www.kispi.uzh.ch/af/ForschungLehre/ClinicalChem.html
Topic Neurometabolic defects in newborns due to splicing errors in the tetrahydrobiopterin (BH4)-biosynthetic gene PTS
Short description
Gene mutations affecting correct pre-mRNA splicing are often found in introns of genes and commonly disrupt the use of constitutive splice sites leading to exon loss. Alternatively, such cis-acting mutations may also activate cryptic splice sites leading to the recruitment of pseudo-exons - often Alu retrotransposons - a phenomenon called exonization. We have identified two new intronic mutations in the PTS gene from a patient with autosomal recessive tetrahydrobiopterin (BH4)-cofactor deficiency. The PTS gene product is required for BH4 biosynthesis, and the cofactor is essential for a number of enzymes including the aromatic amino acid hydroxylases that degrade phenylalanine and synthesize the monoamine neurotransmitter precursors L-dopa and 5-OH-tryptophan. The aim of this thesis is to prove that our newly identified intronic mutations in the PTS are responsible for exon deletion and/or exonization by applying an exon trapping assay with minigene constructs transfected into an indicator cell line.
Supervisor Institute E-mail Phone
Prof. Dr. Beat Thöny Universitäts-Kinderkliniken Zürich, Abteilung für Klinische Chemie und Biochemie, Steinwiesstrasse 75, 8032 Zürich [email protected] 044-266 76 22
Conditions keine
Links www.kispi.uzh.ch/af/ForschungLehre/ClinicalChem.html
Clinical Research Topic Klinische Forschung
Short description
Das Clinical Trials Center des Zentrums für Klinische Forschung verfügt über eine klinische Forschungsstation im UniversitätsSpital Zürich und unterstützt alle Kliniken und Institute bei der Planung und Durchführung klinischer Studien gemäss Good Clinical Practice. Durch aktive Mitarbeit in verschiedenen klinischen Studien sowie in sämtlichen Studienphasen von der Konzeption bis zum Abschluss einer Studie kann die gesamte Methodik der Pharmazeutischen Medizin und Klinischen Forschung erlernt werden.
Supervisor Institute E-mail Phone
PD Dr. Gabriela Senti Clinical Trials Center, Zentrum für Klinische Forschung Universität und Universitätsspital Zürich [email protected] 044 634 55 09
Conditions Selbständiges Arbeiten, Organisations- und Teamfähigkeit, Grundkenntnisse der Klinischen Forschung von Vorteil
Links www.ctc-zkf.usz.ch
Topic „Thermal grill illusion“ – die Illusion von Schmerz
Short description
Die gleichzeitige Applikation von warmen und kühlen nicht schmerzhaften Reizen (z.B. mit der kalten Hand unters warme Wasser) wird als schmerzhaft wahrgenommen (‚Thermal-Grill Illusion‘). Mit dem ‚Thermal Grill‘- Device kann man diese ‚schmerzhafte Illusion‘ in gesunden Personen induzieren. Dieses Projekt beschäftigt sich mit der Frage, ob & in welchem Ausmaß man die TGI an verschiedenen Stellen des Körpers auslösen kann.Training: Studienplanung, -durchführung und -auswertung, Arbeit mit Probanden (evtl. Patienten), Einführung in neurophysiologische Messungen (SSEP, CHEPs, NLG).
Supervisor Institute E-mail Phone
Dr. M. Bolliger, Prof. Dr. A. Curt Uniklinik Balgrist [email protected] 044 386 37 33
Conditions - Bachelorabschluss in Biologie. - Selbständiges Arbeiten; Interesse, etwas Neues zu lernen. - Deutsche Muttersprache und fliessend Englisch
Links http://www.sci-research.uzh.ch/index.html
Topic Pancreatic regeneration and tumorigenesis
Short description
Pancreatitis is a debilitating inflammation of the pancreas and a major risk factor for developing pancreatic cancer, one of the most lethal human malignancy. No effective cure exists for these pancreatic diseases and the lack of effective strategies is linked to the incomplete understanding of their pathophysiology. MSc projects are available to study the molecular mechanisms regulating pancreatic acinar cell replication, a critical step both in the context of regeneration following pancreatitis, and during tumor development. Experimental techniques will include: immunohistochemistry, microscopy, real-time PCR, western blotting, in vitro cell culture.
Supervisor Institute E-mail Phone
Dr. Sabrina Sonda University Hospital Zürich [email protected] 044 255 97 86
Conditions Motivated students with strong background in cell biology are encouraged to apply.
Links http://www.pancreaslab.ch
Circadian Cycles and Sleep Research
Topic Molecular Analysis of Human Biological Clocks
Short description
Our laboratory studies mammalian biological clocks and the molecular pathways by which they control behavior and physiology. Our approach is a broad one, and includes everything from in-vitro biochemistry and cell biology of cultured cells, to transgenic and knock-out mouse models, to genetic and clinical studies involving human beings. More detailed descriptions of current projects, as well as downloadable files of recent publications, can be found on our lab website.
Supervisor Institute E-mail Phone
Prof. Dr. Steven A. Brown Institut für Pharmakologie und Toxikologie [email protected] 044-635-5999
Conditions Knowledge of basic molecular biology techniques. Fluency in English or French.
Links www.sbrownlab.com
Topic The role of the dopaminergic system in sleep-wake regulation
Short description
A sleep deprivation study will be performed on healthy volunteers genotyped for different polymorphisms modulating dopamine levels. They will also be given Tolcapone, used in Parkinson patients to increase dopaminergic tone. The Master’s project will consist of developing a LC-MS protocol for the measurements of Tolcapone and dopamine and its metabolite from salivary samples collected during the study. This work will provide valuable knowledge on the effects of genetic variants on circadian dopamine levels, and their interaction with a potential wake-stimulant drug.
Supervisor Institute E-mail Phone
Prof. Dr. Hans-Peter Landolt Institute of Pharmacology and Toxicology [email protected] 044 – 635 59 53
Conditions interest in pharmacology or chemistry
Links http://www.pharma.uzh.ch/research/chronobiology/areas/psychopharmacology.html
Child Development Center Topic Einfluss von Stress und Bewegung auf die Entwicklung von Kleinkindern
Short description
Im Rahmen eines Nationalfondprojektes wird der Einfluss von Stress und Bewegungsaktivität auf die physische und psychische Entwicklung von Vorschulkindern untersucht.Dazu werden in Zusammenarbeit mit der Universitäten Fribourg und Lausanne verschiedene Aspekte analysiert, u. a. die motorische Entwicklung, die kognitive Leistung, das psychische Wohlbefinden und das Körpergewicht.
Supervisor Institute E-mail Phone
Prof. Dr. Oskar Jenni Entwicklungspädiatrie, Kinderspital Zürich und Institut für Sozial- und Präventivmedizin [email protected] 044 266 77 51
Conditions zuverlässige Arbeitsweise, Freude im Umgang mit Kleinkindern, gute Computerkenntnisse, Basiswissen über statische Analysen
Links http://www.kispi.uzh.ch/Kinderspital/Medizin/Medizin/AWE_de.html http://www.ispm.uzh.ch/aboutus/pdundtp/susikriemler.html
Topic Die Rolle des Schlafes für das emotionale Gedächtnis bei Kindern und Jugendlichen
Short description
Eine Reihe von Studien an Erwachsenen belegen, dass die Speicherung neu gelernter Gedächtnisinhalte massgeblich im Schlaf stattfindet. Das kindliche Gehirn ist nicht nur durch eine hohe Plastizität gekennzeichnet, Kinder schlafen auch länger und wesentlich tiefer als Erwachsene. In der geplanten Studie soll die Rolle des Schlafes für die emotionale Gedächtnisbildung bei Kindern und Jugendlichen untersucht werden. Die Aufgaben eines Masterstudenten/ einer Masterstudentin umfassen die Mithilfe bei der Planung, Durchführung und Datenanalyse.
Supervisor Institute E-mail Phone
Prof. Dr. Reto Huber, Dr. Ines Wilhelm Interdisziplinäres Schlafzentrum, Entwicklungspädiatrie, Kinderspital Zürich [email protected] 044 266 8187
Conditions Flexibilität, Zuverlässigkeit und Verantwortungsbewusstsein, die Bereitschaft, Nächte im Schlaflabor zu verbringen, Interesse an der Arbeit mit Kindern und Jugendlichen
Links www.kispi.uzh.ch/sleep
Child and Adolescent Psychiatry
Topic Brainmapping von Lesenlernen und Aufmerksamkeitsentwicklung
Short description
Lesenlernen, Handlungskontrolle und Aufmerksamkeit sind Meilensteine der kindlichen Entwicklung. Klinische Störungen treten oft schon bei Schulkindern auf (ADHD – Aufmerksamkeitsdefizit – Hyperaktivitätsstörung; Dyslexie – Lesestörung; OCD - Zwangsstörung). In diesem Spannungsfeld untersuchen wir neurophysiologisch Entwicklung, Störungen, und Training von Laut-Schrift-Lernen, Lesen, Aufmerksamkeit und Handlungskontrolle. Die Masterarbeiten in diesen Schwerpunkten erhalten Einblick in funktionelles Brainmapping durch ereignisbezogene EEG Potentiale (ERP) und funktionelle Magnetresonanz (fMRI). Aktuell erforschen wir warum Lesestörungen andauern, vergleichen Zwangsstörungen und ADHD, und messen physiologische Hirnentwicklungen bei ADHD.
Supervisor Institute E-mail Phone
Prof. Dr. Daniel Brandeis, Dr. Silvia Brem Zentrum für Kinder- und Jugendpsychiatrie, Universität Zürich [email protected] 043 556 40 81
Conditions Freude an der Arbeit mit Kindern und Interesse an kognitiven Neurowissenschaften, gute Deutschkenntnisse, Computerkenntnisse, Grundlagenkenntnisse in Statistik, sorgfältige und strukturierte Arbeitsweise
Links http://www.kjpd.uzh.ch/; http://www.neuroscience.uzh.ch/
Tissue Engineering
Topic Muscle Tissue Engineering
Short description
The capability of engineering tissue that is more than a few cubic millimeters in volume requires the formation of new blood capillaries in order to supply essential nutrients and oxygen. Slow Release vasuclar endothelial growth factor (VEGF) administered two weeks prior to cell therapy and muscle cells autonomously expressing VEGF may offer a novel method to deliver angiogenic factors to bioengineered muscles and improve volume preservation and functional outcome.The master thesis will imply determination of feasibilty of modifying Muscle Precursor Cells to release VEGF and determination the phenotypic and functional characteristics of engineered muscle tissue supplemented with VEGF in a mouse model.
Supervisor Institute E-mail Phone
PD Dr. Daniel Eberli University Hospital Zürich, Urology Department [email protected] 044 255 93 01
Conditions -
Links http://www.urologie.usz.ch/LehreUndForschung/Grundlagenforschung/Projekte/Seiten/Tissue-Engineering.aspx
Topic Heart valve tissue engineering
Short description
One of the promising projects of our Regenerative Medicine Program is the tissue engineering of heart valves to overcome the drawbacks of currently used mechanical or bioprosthetic valves for treating valvular heart disease. Our major goal is to develop a clinically relevant tissue engineered living heart valve, with the capacity of regeneration and growth which can be implanted by minimally invasive catheter technology. A highly interdisciplinary approach combines basic sciences, medical research, engineering and clinical practice. This large promising ongoing project currently embraces smaller in-vitro and in-vivo studies to further optimize the final end product. Master projects can be designed to fit the interest and qualities of the student and could be related to for example optimization of cell sources, culture process, in-vivo remodeling, etc. Students will have the opportunity to be trained in (among others) the following techniques: literature review, project planning, cell culture, tissue engineering, histology, quantification of extracellular matrix, analyzing results, reporting and presenting their results.
Supervisor Institute E-mail Phone
Prof. Dr. Simon P. Hoerstrup Regenerative Medicin Program; Division of Surgical Research; University and University Hospital Zurich [email protected], [email protected] 044 6345687
Conditions Motivated students in the area of biology, biomedical sciences or equivalent are encouraged to send applications. Experience with cell cultures is an advantage.
Links www.ReMedi.uzh.ch
Immunology
Topic Mechanisms of Inflammation
Short description
Macrophages play a pivotal role in inflammatory processes. They respond to inflammatory mediators such as microbial agents or chemokines and themselves produce and release pro-inflammatory cytokines and chemokines, thereby communicating with other cell types such as vascular endothelial cells. We investigate novel inflammatory signaling pathways as well as anti-inflammatory mechanisms in human macrophages aiming to a better understanding of severe diseases such as sepsis/septic shock, macrophage activation syndromes, and unusual host responses to microbial pathogens. Various MSc projects are available, cutting edge technologies in cell and molecular biology will be applied in all projects.
Supervisor Institute E-Mail Phone
Prof. Dr. G. Schoedon-Geiser, and research group leaders Molecular and Clinical Inflammation Research Unit Department of Medicine, University Hospital of Zürich [email protected]
Conditions Completed bachelor level with outstanding grades (5 or higher), special interest in cell and molecular biology
Links http://www.research-projects.uzh.ch/a1568.htm Topic Neuroimmunology
Short description
A) Virtually all disorders of the nervous system display an inflammatory component. To this day it remains unclear whether the inflammation coinciding with damage mediated by CNS-intrinsic degenerative effects is destructive or regenerative. Using in vivo models and immune based methodology, several projects in this direction are being pursued. B) Autoimmune disorders of the CNS are mediated by the conduct of adaptive and innate immunity against „self”. The nature of this immune response remains ill-defined and we seek a better understanding of encephalitogenic immunity
Supervisor Institute E-mail Phone
Prof. Dr. Burkhard Becher University of Zurich, University Hospital Institute of Experimental Neuroimmunology Y44-J7 (Office), J38/42 (Lab) Winterthurerstrasse 190 CH-8057 Zürich [email protected] +41-44-635-3703 Fax: +41-44-635-6883
Conditions Solid understanding of basic immunology and molecular biology. Good communication skills. Interest to work in a highly motivated, vibrant and young research environment
Links http://www.neuroimmunology.uzh.ch
Infectiology
Topic Infectiology: from veterinary to human medicine
Short description
Infectious diseases are of great importance; increased mobility leads to fast spread of infectious agents and climate changes are associated with emerging diseases. Infections of wild, pet and farm animals may have a zoonotic potential. In addition, animal models serve as an important prerequisite to investigate therapeutic interventions or immunoprophylactic strategies. Various veterinary infectiology projects with relevance for human medicine are available; projects include a variety of molecular, cell culture and/or serological methods.
Supervisor Institute E-mail Phone
Prof. Dr. Regina Hofmann-Lehmann Clinical Laboratory, Vetsuisse Faculty [email protected] 044 635 83 22
Conditions Candidates should be highly motivated, committed and good team players.
Links www.vetlabor.uzh.ch
Toxicology
Topic Investigating the role of soluble epoxide hydrolase in lipid metabolism
Short description
Mammalian soluble epoxide hydrolase (sEH) comprises an epoxide hydrolase as well as a phosphatase activity, both involved in lipid metabolism. The sEH is implicated in cardio-vascular and inflammatory diseases, mostly based on the metabolism endogenous epoxides by the EH. The phospha-tase turns over intermediates of the cholesterol biosynthesis pathway. We will analyse the role of sEH in sterol and lipid homeostasis to investigate the mechanistic link between these two sEH activities. Techniques: mass spectrometry, cell culture, molecular biology and protein biochemistry
Supervisor Institute E-mail Phone
Prof. Dr. Michael Arand Institute of Pharmacology and Toxicology [email protected] 044 635 59 79
Conditions -
Links http:// www.pharma.uzh.ch
Neurophysiology
Topic Molecular Neurobiology: Understanding the role of Gephyrin in GABAA receptor clustering at synapses.
Short description
GABAA receptors are major inhibitory receptors in the brain. Defects in GABAA receptor function has been implicated in epilepsy, depression, alcohol addiction etc. Hence, its important to understand how the receptors are brought to the synapse and maintained at the synapses. The research project will involve studying the interaction of GABAA receptor subunits with the scaffolding protein gephyrin and its implications on synaptic clustering of the receptor. The project will use techiques in Molecular biology, biochemistry, cell culture and fluorescence microscopy.
Supervisor Institute E-mail Phone
Prof. Dr. Jean-Marc Fritschy/ Dr. Shiva Tyagarajan Pharmacology and Toxicology [email protected] 044-63 55937
Conditions I am looking for a motivated and interested person who will fit into our fun and friendly lab environment.
Links http://www.pharma.uzh.ch/neuromorpho_website/index.html
Topic Eco-evolutionary adaptations of adult hippocampal neurogenesis
Short description
New neurons are continuously added into the hippocampus of most mammals. The functional relevance of these new neurons is still debated. Apparently, both evolutionary constraints as well as species-specific requirements for behavioral flexibility influence the magnitude of cell proliferation and neuronal integration. We are interested in the phenomenon of adult neurogenesis from a broad, comparative view. We investigate various small to medium sized mammels, in the wild and also in the laboratory. Each master project concentrates on one species, investigating behavioral performance (ecological niche, learning, physical exercise), followed by the estimation of the number of immunohistologically identified cell types in the hippocampus.
Supervisor Institute E-mail Phone
Dr. Irmgard Amrein Institute of Anatomy [email protected] 044/635 5342
Conditions Bachelor in Biology, neuroscience knowledge required
Links http://www.anatom.uzh.ch/research/DivisionWolfer_en.html
Topic Automatisierte Erfassung von Lernverhalten bei Mäusen im Sozialverband
Short description
Unsere Gruppe setzt selbst entwickelte Lernkäfige (IntelliCage) ein, die mit einem Minimum an Stress kognitive Fähigkeiten von Mäusen im Gruppenkäfig erfassen und speichern. Für eine Masterarbeit erwarten wir, dass die individuellen Fähigkeiten der Tiere sowohl im automatisierten System als auch in klassischen Einzeltests für Lernverhalten (z.B. Schwimmnavigation, Vermeidungslernen) erfasst werden. Die Daten werden dann miteinander und allenfalls mit neuronanatomischen Parametern korreliert.
Supervisor Institute E-mail Phone
Prof. Dr. David P. Wolfer, Anatomisches Institut, Universität Zürtich-Irchel [email protected] 044/63 55360
Conditions Interesse an Verhaltensforschung mit Mäusen, Computerkenntnisse, Grundkenntnisse Statistik
Links http://www.uzh.ch/anatom/forschung/f_forschung_e.htm
Neuropathology
Topic Various MSc Projects in Prion Science
Short description
Various MSc project are available in the realm of prion science. It is possible to choose between projects dealing with (1) diagnosis of human prion diseases, (2) prion immunology, (3) prion genetics, and (4) prion models in simple genetically tractable organisms (fruitfly, worm). Candidates should have an outstanding academic track record: for Swiss candidates, all grades must be 5 or higher. Candidates should be prepared to commit themselves fully to their thesis in a very demanding research environment.
Supervisor Institute E-mail Phone
Prof. Dr. Adriano Aguzzi Institut für Neuropathologie [email protected]
Conditions 100% commitment. Above-average grades in molecular biology.
Links http://www.uzh.ch/pathol/neuropathologie/index.html
Psychiatry Research
Topic Alzheimer’s disease - Molecular pathology and the development of immunotherapy.
Short description
Transgenic mouse models will be used to analyze mechanisms of toxicity and neurodegeneration, and to develop novel approaches for treatment. A particular focus is the development of immunotherapy designed to reduce beta-amyloid-related neurotoxicity in transgenic mice..
Supervisor Institute E-mail Phone
Prof. Dr. Roger Nitsch University of Zurich Division of Psychiatry Research [email protected] 044 634 8871
Conditions Basic knowledge of molecular biology
Links http://www.dpr.uzh.ch/ http://www.nccr-neuro.uzh.ch/resp2.html http://www.neurozh.ch/e/groups/nitsch00.htm
Evolutionary Medicine
Topic Evolutionary perspectives of human disease Short description
Evolutionary perspectives of human health and disease can be studied on a macroscopic or molecular level. For example, musculoskeletal disorders are extremely common in modern people. One factor in their aetiology is biomechanical stress, part of which might be directly attributable to modern daily behavior. Using radiographs, comparative anatomical studies and analyses of the fossil / skeletal and soft tissue record help to investigate important etiological disease factors. Using ancient DNA techniques from a number of archaeological contexts e.g. mitochondrial DNA sequences can be compiled and also functional gene data can be compared across different regions and time period. Such molecular analyses allow e.g. to achieve a more complete picture of the evolutionary dynamics of human immunity and pathogen susceptibility. Opportunities for Master theses exist in different areas of the interdisciplinary research field.
Supervisor Institute E-mail Phone
Prof Dr. Dr. F. Rühli Institute of Evolutionary Medicine, University Zürich-Irchel [email protected] 044 635 01 11
Conditions None (for aDNA projects own DNA needs to be analysed to be able to rule out modern contamination)
Links http://www.iem.uzh.ch/