material safety data sheet - s3.amazonaws.com08-22-2012]…material safety data sheet according to...

Material Safety Data Sheet

Catalog Number: 61-I23MS-E01

Mouse IL-23 ELISA

+ Europe + USA eBioscience eBioscience, Inc. Campus Vienna Biocenter 2 10255 Science Center Drive 1030 Vienna, Austria San Diego, CA 92121 technical support: +43 (1) 796 40 40-120 technical support: +1 (888) 810 6168 customer service: +43 (1) 796 40 40-304 customer service: +1 (888) 999 1371 fax: +43 (1) 796 40 40-400 fax: +1 (858) 642 2046 [email protected] [email protected] [email protected] [email protected] www.ebioscience.com www.ebioscience.com

RLetter of Safety Hazard Exemption Product Name: Mouse IL-23 Platinum ELISA Catalog Number: BMS6017 Our review of the manufacturing process for the requested item indicates that, apart from the components listed below, it is exempt from MSDS requirement. This product contains neither hazardous substances to health or the environment at concentrations ≥ 1% nor PBT/ vPvB* substances at concentrations ≥ 0.1 %. As a result, a Material Safety Data Sheet is not required for this product. For further information refer to following regulations:

European Regulation 1907/2006 Article 31 United States 29CFR 1910.1200

Although this product is considered a general laboratory reagent, we recommend that Good Laboratory Practices (GLP) be followed to ensure the safe use and disposal of this reagent. Kit components which require Material Safety Data Sheets:

Stop Solution 1N NaOH 1N HCl Substrate Solution Propidium Iodide

For these Material Safety Data Sheets please refer to our Homepage www.ebioscience.com or alternatively they can be requested from our Customer Service at [email protected]. Sincerely,

Wolfgang Angermann Quality Manager

* PBT-substances: persistent, bioaccumulative and toxic substances vPvB-substances: very persistent, very bioaccumulative substances

http://www.ebioscience.com/

mailto:[email protected]

Material Safety Data Sheet according to EC 1907/2006

Trade name: Stop Solution

+ Europe + USA Bender MedSystems GmbH eBioscience, Inc. Campus Vienna Biocenter 2 10255 Science Center Drive 1030 Vienna, Austria San Diego, CA 92121 phone: +43 (1) 796 40 40-0 technical support: +1 (888) 810 6168 fax: +43 (1) 796 40 40-400 customer service: +1 (888) 999 1371 fax: +1 (858) 642 2046 [email protected] [email protected] [email protected] [email protected] www.bendermedsystems.com www.ebioscience.com

Version: 01 / EN Date: 08/02/2010 Revised date: -

1. IDENTIFICATION OF THE PREPARATION AND OF THE COMPANY/UNDERTAKING

1.1 Identification of the preparation: Identification on the label / trade name: Stop Solution Additional identification: 1M phosphoric acid, phosphoric acid 9%, 1M H3PO4 1.2 Use of the preparation: The stop solution is used to terminate an enzyme reaction. 1.3 Company/undertaking identification: Bender MedSystems GmbH Campus Vienna Biocenter 2 1030 Vienna, Austria phone: +43 1 796 40 40 100 fax: +43 1 796 40 40 400

E-Mail: [email protected] Information contact: [email protected] 1.4 EMERGENCY TELEPHONE: phone: +43 1 796 40 40 100 (Austria) phone: +1 (888) 999 1371 (USA) These phone numbers are available during office hours.

2. HAZARDS IDENTIFICATION 2.1 Hazards description: Causes corrosion/ irritation 2.1.1 Classification:

Regulation (EC) No 1272/2008 [CLP]

Hazard classes/Hazard categories Hazard statement 3.1/ category 4 3.2/ Category 2 3.3/ Category 2 4.1/ Category 4

H302, H312 H315 H319 H413





3.3 Remark: Full text of H- and R-phrases: see section 16. 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Preparation / mixture related informations Description: Mixture of mentioned below ingredients: 3.2 Ingredients:

Chemical name EC-No Index-No CAS-No Amount (%)

Classification according Regulation (EC) No 1272/2008 [CLP]

Classifcation according 67/548/EEC

Hazard class/ Hazard categories

Hazard- statement

Phosphoric Acid Water, dest.

231-633-2 231-791-2

015-011-00-6 -

7664-38-2 7732-18-5

99 -

3.2/ Category 1B -

H314 -

C; R34 -

4. FIRST AID MEASURES 4.1 General informations: Skin- and eyecontact causes irritation In case of indisposition contact a doctor, show this datasheet. 4.2 In case of skin contact: Wash affected bodyparts with plenty amount of water. 4.3 In case of eye contact: Wash the affected eye with water for a minimum of 10 minutes. In case of indisposition contact an eye-specialist.

4.4 In case of ingestion: Immediately wash your mouth with plenty of water.

Furthermore swallow water in small amounts (dilution)

67/548/EEC or 1999/45/EC

Hazards characteristics R-Phrases Xi R36, R38, R53





5. FIRE-FIGHTING MEASURES 5.1 Suitable extinguishing media: water, dry powder extinguisher, carbondioxid 5.2 Extinguishing media which must not be used for safety reasons: Attention with dry powder extinguisher.

It’s not appropriate for in door fire because raised foam causes lack of sight.

5.3 Special exposure hazards arising from the substance or preparation itself, combustion products, resulting gases:

In case of fire, despite flue gas also dangerous, product specific gases might be produced (phosphorus oxide). Because of the very low concentration of this mixture it is very unlikely that above mentioned oxides will be produced in a hazered amount. Further data are not known.

5.4 Special protective equipment for fire-fighters: In case of heavy smoke a respirator shall be used. 6. ACCIDENTAL RELEASE MEASURES 6.1 Personal precautions: In case of spilled mixture safety gloves have to be worn to prevent skin contact. In case of big amounts an additional inhalation protection is recommended. 6.2 Environmental precautions:

Mixture must not discard in sewage system/ residual waste. Dilute residues cautiously with water and clean it up with a paper towel. 6.3 Methods for cleaning up:

Spilled mixture can be cleaned up with a paper towel an discarded in an appropriate waste.

Afterwards clean bench with water.





7. HANDLING AND STORAGE 7.1 Handling Advices on safe handling: Protective measures: wear protective clothing Precautions against fire and explosion: Development of explosive atmosphere is not possible 7.2 Storage Technical measures and storage conditions:

Storage in well closed containers Packaging materials: Acid resistant containers (e.g.: glas, polyethylen) are suitable Requirements for storage rooms and vessels: Prevent direct sunlight and heat. Store in well aired storage rooms. Further information on storage conditions: Storage temperature: -20°C – 8°C Storage stability: stabil Maximal storage periode: 2 years 8. EXPOSURE CONTROLS / PERSONAL PROTECTION 8.1 Exposure limit values: 8.1.1 Components with occupational exposure limits requiring monitoring:

Phosphoric acid: maximum allowable concentration

EU: short term value: 2 mg/m3 long term (8 hs): 1 mg/m3

USA: short term value no data available long term (8 hs): 1 mg/m3





8.2 Personal protection equipment: Respiratory protection: not necessary Hand protection: disposable protective gloves Eye protection: not necessary Body protection: lab coat, lab shoes 9. PHYSICAL AND CHEMICAL PROPERTIES 9.1 Appearance Physical state: liquid Colour: colourless Odour: odourless 9.2 Important health, safety and environmental information 9.2.1 Safety relevant data Value Remark_ pH (20 °C): pH 2, acid pH paper Melting point (°C): about 150°C Boiling point (°C): no data Ignition temperature (°C): no data Vapour pressure (°C): no data Density (g/cm3): no data Water solubility: very well soluble

Viscosity, dynamic (mPa s): no data Explosion limits: - mixture is not explosive 10. STABILITY AND REACTIVITY stable under mentioned storage conditions 10.1 Conditions to avoid:

Direct sunlight, high temperature





10.2 Materials to avoid: Bases (cause heat development), powdery metals

(heat developemet and dynamics of the reaction depends on the acid concentration)

10.3 Hazardous decomposition products: No data available

Because of the low acid concentration in the mixture, no hazardous gases are expected.

11. TOXICOLOGICAL INFORMATION 11.2 Acute effects (toxicity tests)

Specific symptoms in animal studies:

In case of ingestion: Somnolence, Hematuria In case of skin contact: Somnolence, Infuriation In case of inhalation: no data In case of eye contact: no data

Irritant and corrosive effects:

Sensitisation In case of skin contact: no data

In case of inhalation: no data

Repeated dose toxicity (sub-acute, sub-chronic, chronic) No data available

7664-38-2 phosphoric acid Effect dose Species

Acute oral toxicity LD50 = 1530 mg/kg rat

Acute dermal toxicity LD50 = 2730 mg/kg rat

Acute inhalative toxicity LC50 = 850 mg/m3 rat

Exposure time

Species Evaluation

Primary irritation to the skin: 24 h rabbit strong irritation

Irritation to eyes no data rabbit strong irritation





CMR effects (carcinogenity, mutagenicity and toxicity for reproduction)

No CMR effects 12. Ecological information 12.1 Ecotoxicity:

no data 12.2 Mobility: Known or predicted distribution to environmental compartments:

In case of shifting the pH value there might be some damaging effects for aquatic organism. The mixture must not dispose in the sewage system without pretreatment.

13. DISPOSAL CONSIDERATIONS 13.1 Appropriate disposal / Product:

All national and local laws have to be considered. This product is only allowed to be discarded by a licensed waste management company.

13.2 Waste codes / waste designations according to EWC / AVV (Austria): Medical waste: Waste code: 97101 In other countries different conditions might be valid. All national and local laws

have to be considered. 13.3 Appropriate packaging: Rinse container with water, dispose as the product.

14. TRANSPORT INFORMATION Official transport designation: Stop Solution, Research reagent

Because product is no dangerous good, no specific codes or labes are necessary.

15. REGULATORY INFORMATION





15.1 EU regulations

Labelling (Regulation (EC) No 1272/2008) Hazard components for labelling: Phosphoric Acid Signal words: Attention Hazard pictograms: GHS07

Hazard statements: H302, H312, H315, H319, H413 Precautionary statements: P281, P301, P302, P305, P330, P404, P410 16. OTHER INFORMATION 16.1 Relevant R- and H-phrases: H302: Harmful if swallowed H312: Harmful in contact with skin H315: Causes skin irritation H319: Causes serious eye irritation H413 May cause long lasting harmful effects to aquatic life R36: Irritating to eyes R38: Irritating to skin R53: May cause long-term adverse effects in the aquatic environment P281: Use personal protective equipment as required P301: IF SWALLOWED: swallow water in small amounts P302: IF ON SKIN: wash with water P305: IF IN EYES: wash with water P330: Flush mouth P404: Store in a closed container P410: Protect from sunlight





16.2 Training instructions: no data 16.4 Data sources: BDI Helpdesk (Germany), ECHA, GESTIS (IFA) 16.5 Further information:

The above information has been assembled to the best of our knowledge but might not be complete. Bender MedSystems GmbH can not be held liable for any damages resulting from handling with the product


Trade name: 1N Sodiumhydroxide solution, 1N NaOH




1.1 Identification of the preparation:

Identification on the label / trade name: 1N Sodiumhydroxide solution, 1N NaOH

Additional identification: NaOH 4% 1.2 Use of the preparation: The NaOH is used to neutralize acidified samples. 1.3 Company/undertaking identification: Bender MedSystems GmbH Campus Vienna Biocenter 2 1030 Vienna, Austria phone: +43 1 796 40 40 100 fax: +43 1 796 40 40 400


2. HAZARDS IDENTIFICATION 2.1 Hazards description: Causes corrosion/ irritation





2.1.1 Classification:

2.1.2 Remark: Full text of H- and R-phrases: see section 16. 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Preparation / mixture related informations Description: Mixture of mentioned below ingredients: 3.2 Ingredients:





Hazard- statement

NaOH Water, dest.

215-185-5 231-791-2

011-002-00-6 -

1310-73-2 7732-18-5

solid -

3.2/ Category 1 -

H314 -

C; R34 -

4. FIRST AID MEASURES 4.1 General informations: In case of longer exposer contact a doctor, show this datasheet.

Skin- and eyecontact causes corrosion. 4.2 In case of skin contact: Wash affected bodyparts with plenty amount of water. In case of constant skin irritation contact a doctor.


Hazard classes/Hazard categories Hazard statement 3.1/ Category 4 3.2/ Category 2 3.3/ Category 2 4.1/ Category 3

H302, H312 H315 H319 H412

67/548/EEC or 1999/45/EC






4.3 In case of eye contact: Wash the affected eye with water for a minimum of 10 minutes. Contact an eye-specialist.


Furthermore swallow water in small amounts (dilution). Don’t cause vomiting. Contact a doctor.




In case of fire, despite flue gas also dangerous, product specific gases might be produced. Because of the very low concentration of this mixture it is very unlikely that oxides will be produced in a hazardous amount. Further data are not known.


Mixture must not discard in sewage system/ residual waste. Dilute residues cautiously with water and clean it up with a paper towel.





6.3 Methods for cleaning up:


Afterwards clean bench with water. 7. HANDLING AND STORAGE 7.1 Handling Advices on safe handling: Protective measures: wear protective clothing Precautions against fire and explosion: Development of explosive atmosphere is not possible 7.2 Storage Technical measures and storage conditions:

Storage in well closed containers Packaging materials: Base resistant containers (e.g.: polyethylen) are suitable Containers made of aluminium, tin, zinc are not suitable. Requirements for storage rooms and vessels: Prevent direct sunlight and heat. Store in well aired storage rooms. Further information on storage conditions: Storage temperature: 2 – 8°C Storage stability: stabil Maximal storage periode: 2 years 8. EXPOSURE CONTROLS / PERSONAL PROTECTION 8.1 Exposure limit values: 8.1.1 Components with occupational exposure limits requiring monitoring:





Sodiumhydroxide solution: maximum allowable concentration

Austria: short term value: 2 mg/m3 long term (8 hs): 4 mg/m3

USA: short term value no data available long term (8 hs): 2 mg/m3

8.2 Personal protection equipment: Respiratory protection: only necessary in case of fumes Hand protection: disposable protective gloves Eye protection: not necessary Body protection: lab coat, lab shoes 9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Appearance Physical state: liquid Colour: colourless Odour: odourless 9.2 Important health, safety and environmental information 9.2.1 Safety relevant data Value Remark_ pH (20 °C): pH 10, alkaline pH paper Melting point (°C): no data Boiling point (°C): no data Ignition temperature (°C): no data Vapour pressure (°C): no data

Density (g/cm3): 1.0318 g/cm3 Water solubility: very well soluble

Viscosity, dynamic (mPa s): no data Explosion limits: - mixture is not explosive 10. STABILITY AND REACTIVITY stable under mentioned storage conditions





10.1 Conditions to avoid:


10.2 Materials to avoid: Metals (development of hydrogen, explosion hazard) Acids (heat development) Sal ammonic (development of ammoniac) 10.3 Hazardous decomposition products: No data available 11. TOXICOLOGICAL INFORMATION 11.2 Acute effects (toxicity tests)


No data available Irritant and corrosive effects:

Sensitisation

In case of skin contact: no data In case of inhalation: no data


1310-73-2/ NaOH (waterfree NaOH) Effect dose Species

Acute oral toxicity LD50 = 2000 mg/kg rat

Acute dermal toxicity no data

Acute inhalative toxicity no data

Exposure time

Species Evaluation

Primary irritation to the skin: no data rabbit corrosion

Irritation to eyes no data rabbit corrosion







12.2 Mobility: Known or predicted distribution to environmental compartments:




13.2 Waste codes / waste designations according to EWC / AVV (Austria): Medical waste: Waste code: 97101 In other countries different conditions might be valid. All national and local laws

have to be considered. 13.3 Appropriate packaging: Rinse container with water, dispose as the product.

Aquatic toxicity Effectdose Exposure time Species Acute fish toxicity LC50=45.4 mg/l 96 h Onchorhynchus mykiss

Acute daphnia toxicity EC50=76 mg/l 24 h Daphnia magna

Acute algae toxicity No data





14. TRANSPORT INFORMATION

Official transport designation: 1N NaOH, 1N Sodiumhydroxyde solution Because product is no dangerous good, no specific codes or labes are

necessary. 15. REGULATORY INFORMATION 15.1 EU regulations

Labelling (Regulation (EC) No 1272/2008) Hazard components for labelling: Sodiumhydroxide solution, NaOH Signal words: Attention Hazard pictograms: GHS07

Hazard statements: H302, H312, H315, H319, H412 Precautionary statements: P281, P301, P302, P305, P330, P404, P410 16. OTHER INFORMATION 16.1 Relevant R- and H-phrases: H302: Harmful if swallowed

H312: Harmful in contact with skin H315: Causes skin irritation H319: Causes serious eye irritation

H412 Harmful to aquatic life with long lasting effects R36: Irritating to eyes R38: Irritating to skin R53: May cause long-term adverse effects in the aquatic environment





P281: Use personal protective equipment as required P301: IF SWALLOWED: swallow water in small amounts P302: IF ON SKIN: wash with water P305: IF IN EYES: wash with water P330: Flush mouth P404: Store in a closed container P410: Protect from sunlight 16.2 Training instructions: no data 16.4 Data sources: REACH Helpdesk (Austria), BDI Helpdesk Germany), ECHA, GESTIS (IFA) 16.5 Further information:



Trade name: 1N Hydrochloric acid, 1N HCl




1.1 Identification of the preparation: Identification on the label / trade name: 1N Hydrochloric acid, 1N HCl Additional identification: ca. 3% HCl 1.2 Use of the preparation: The hydrochloric acid is used to acidify the samples. 1.3 Company/undertaking identification: Bender MedSystems GmbH Campus Vienna Biocenter 2 1030 Vienna, Austria phone: +43 1 796 40 40 100 fax: +43 1 796 40 40 400

E-Mail: [email protected] Information contact: [email protected] National contact: Customer Service 1.4 EMERGENCY TELEPHONE: phone: +43 1 796 40 40 100 (Austria) phone: +1 (888) 999 1371 (USA) These phone numbers are available during office hours.

2. HAZARDS IDENTIFICATION 2.1 Hazards description: Causes corrosion/ irritation





2.1.1 Classification:






Hazard- statement

Hydrochloric Acid Water, dest.

231595-7 231-791-2

017-002-01-X -

7647-01-0 7732-18-5

32 -

3.2/ Category 1B -

H314 -

C; R34 -


Hazard classes/Hazard categories Hazard statement 3.1/ category 4 3.2/ Category 2 3.3/ Category 2 4.1/ Category 4

H302, H312 H315 H319 H413

67/548/EEC or 1999/45/EC






4. FIRST AID MEASURES 4.1 General informations: Skin- and eyecontact causes irritation In case of indisposition contact a doctor, show this datasheet. 4.2 In case of skin contact: Wash affected bodyparts with plenty amount of water. 4.3 In case of eye contact: Wash the affected eye with water for a minimum of 10 minutes. In case of indisposition contact an eye-specialist.


Furthermore swallow water in small amounts (dilution)




In case of fire, despite flue gas also dangerous, product specific gases might be produced. Because of the very low concentration of this mixture it is very unlikely that oxides will be produced in a hazered amount. Further data are not known.

5.4 Special protective equipment for fire-fighters: In case of heavy smoke a respirator shall be used.





6. ACCIDENTAL RELEASE MEASURES 6.1 Personal precautions: In case of spilled mixture safety gloves have to be worn to prevent skin contact. In case of big amounts an additional inhalation protection is recommended. 6.2 Environmental precautions:




Storage in well closed containers Packaging materials: Acid resistant containers (e.g.: glas, polyethylen) are suitable Containers made of metal are not suitable.





Requirements for storage rooms and vessels: Prevent direct sunlight and heat. Store in well aired storage rooms. Further information on storage conditions: Storage temperature: 2 – 8°C Storage stability: stabil Maximal storage periode: 2 years 8. EXPOSURE CONTROLS / PERSONAL PROTECTION 8.1 Exposure limit values: 8.1.1 Components with occupational exposure limits requiring monitoring:

Hydrochloric Acid: maximum allowable concentration

EU: short term value: 15 mg/m3 long term (8 hs): 8 mg/m3

USA: short term value 7 mg/m3 long term (8 hs): no data available

8.2 Personal protection equipment: Respiratory protection: not necessary Hand protection: disposable protective gloves Eye protection: not necessary Body protection: lab coat, lab shoes 9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Appearance Physical state: liquid Colour: colourless Odour: odourless





9.2 Important health, safety and environmental information 9.2.1 Safety relevant data Value Remark_ pH (20 °C): pH 2, acid pH paper Melting point (°C): no data Boiling point (°C): no data Ignition temperature (°C): no data Vapour pressure (°C): no data

Density (g/cm3): 1.0130 g/cm3 Water solubility: very well soluble



10.2 Materials to avoid: No data available 10.3 Hazardous decomposition products: No data available









No data available Sensitisation In case of skin contact: no data


Repeated dose toxicity (sub-acute, sub-chronic, chronic) No data available CMR effects (carcinogenity, mutagenicity and toxicity for reproduction)




7647-01-0 HCl Effect dose Species

Acute oral toxicity LD50 = 900 mg/kg rabbit


Acute inhalative toxicity LC50 = 3124 mg/l rat

Aquatic toxicity (1N HCl) Effectdose Exposure time Species Acute fish toxicity LC50=826 mg/l 96 h Leuciscus idus

Acute daphnia toxicity No data

Acute algae toxicity No data







13.2 Waste codes / waste designations according to EWC / AVV (Austria): Medical waste: Waste code: 97101

In other countries different conditions might be valid. All national and local laws have to be considered.

13.3 Appropriate packaging: Rinse container with water, dispose as the product. 14. TRANSPORT INFORMATION

Official transport designation: 1N HCl, 1N Hydrochloric acid

Because product is no dangerous good, no specific codes or labes are necessary.

15. REGULATORY INFORMATION 15.1 EU regulations

Labelling (Regulation (EC) No 1272/2008) Hazard components for labelling: Hydrochloric Acid Signal words: Attention Hazard pictograms: GHS07





Hazard statements: H302, H312, H315, H319, H413 Precautionary statements: P281, P301, P302, P305, P330, P404, P410 16. OTHER INFORMATION 16.1 Relevant R- and H-phrases:

H302: Harmfull if swallowed H312: Harmfull in contact with skin H315: Causes skin irritation

H319: Causes serious eye irritation H413 May cause long lasting harmful effects to aquatic life R36: Irritating to eyes R38: Irritating to skin R53: May cause long-term adverse effects in the aquatic environment





P281: Use personal protective equipment as required P301: IF SWALLOWED: swallow water in small amounts P302: IF ON SKIN: wash with water P305: IF IN EYES: wash with water

P330: Flush mouth P404: Store in a closed container P410: Protect from sunlight 16.2 Training instructions: no data 16.4 Data sources: BDI Helpdesk Germany), ECHA, GESTIS (IFA) 16.5 Further information:



Trade name: Substrate Solution




1.1 Identification of the preparation: Identification on the label / trade name: Substrate Solution Additional identification: TMB 1.2 Use of the preparation:

This preparation causes a coloured reaction whose intensity depends on the concentration of the substance which has to be determined.

1.3 Company/undertaking identification: Bender MedSystems GmbH Campus Vienna Biocenter 2 1030 Vienna, Austria phone: +43 1 796 40 40 100 fax: +43 1 796 40 40 400


2. HAZARDS IDENTIFICATION Hazards description:

According to the regulation there are no hazardous substances. Therefore no classification and labelling is necessary.

3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Preparation / mixture related informations

Description: Mixture of tetramethylbenzidin TMB, hydrogen peroxide, detergent Tween 20, citrate, EDTA, preserving agent kathon CG.





3.2 Hazard Ingredients:

Chemical name EG-Nr. CAS-Nr. Amount (%) in the preperation

Classification according Regulation (EC) No 1272/2008 [CLP] (Related to the conc. form)

Classifcation according 67/548/EEC (related to the conc. form)


Hazard- statement

3,3‘,5,5‘-Tetramethylbenzidin EDTA 5-Chlor-2-methyl-4-isothiazolin-3-on 2-Methyl-4-isothiazolin-3-on Hydrogen peroxide

2593646 2053583 N/ A N/ A 231-765-0

54827-17-7 6381-92-6 26172-55-4 2682-20-4 7722-84-1

< 0.04 < 0.1 < 0.0001 < 0.00004 < 0.005

3.1/4 3.2/2 3.1/3; 4.1/1 3.1/3; 4.1/1 2.13/1; 3.1/4; 3.2/1A

H302,312,332 H315 H301,311,331,410 H301,311,331,410 H271,302,314,332

Xn; R20/21/22-36/37/38-40 Xi; R36 T, N; R23/24/25-34-43-50/53 T, N; R23/24/25-34-43-50/53 O, C; R: 5-8-20/22-35

4. FIRST AID MEASURES 4.1 General informations: Because of the low concentrations of the ingredients seeing a

doctor is not necessary. 4.2 In case of skin contact: Wash affected bodyparts with plenty amount of water. 4.3 In case of eye contact: Wash the affected eye with water for 10 minutes. 4.4 In case of ingestion: Immediately wash your mouth with plenty of water.

Furthermore swallow water in small amounts (dilution).







5.3 Special exposure hazards arising from the substance or preparation itself, combustion products, resulting gases: The Substrate Solution itself is not flammable.

In case of fire usual flue gas will be produced. Because of the very low concentration of this preperation it is very unlikely that gases in a hazered amount will be produced. Further data are not known.

5.4 Special protective equipment for fire-fighters: In case of heavy smoke a respirator shall be used. 6. ACCIDENTAL RELEASE MEASURES 6.1 Personal precautions: In case of spilled mixture safety gloves have to be worn to prevent skin contact. 6.2 Environmental precautions:




Storage in light-proof, well closed containers.





Packaging materials: Containers made of polyethylen are suitable. Requirements for storage rooms and vessels: Prevent direct sunlight and heat. Store in well aired storage rooms. Further information on storage conditions: Storage temperature: -20°C - 8°C Storage stability: stabil Maximal storage periode: 12 months 8. EXPOSURE CONTROLS / PERSONAL PROTECTION 8.1 Exposure limit values: 8.1.1 Components with occupational exposure limits requiring monitoring:

maximum allowable concentration

� TMB: Austria, EU, USA: no data available � EDTA: Austria, EU, USA: no data available

� � 5-Chlor-2-methyl-4-isothiazolin-3-on:

Austria: short term value: no data available long term (8hs): 0.05 mg/m3

USA: short/ long term: no data available

� 2-Methyl-4-isothiazolin-3-on: Austria: short term value: no data available long term (8hs): 0.05 mg/m3

USA: short/ long term: no data available

� Hydrogen peroxide: Austria: short term value: 2.8 mg/m3 long term (8hs): 1.4 mg/m3

USA: short term value: no data available long term (8hs): 1.4 mg/m3





8.2 Personal protection equipment: Respiratory protection: not necessary Hand protection: disposable protective gloves Eye protection: not necessary Body protection: lab coat, lab shoes 9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Appearance Physical state: liquid Colour: yellowish or bluish Odour: odourless 9.2 Important health, safety and environmental information 9.2.1 Safety relevant data Value Remark_ pH (20 °C): about pH 3, acid pH paper Melting point: (°C): no data Boiling point (°C): no data Ignition temperature (°C): no data Vapour pressure (°C): no data

Density (g/cm3): no data Water solubility: very well soluble


Direct sunlight, high temperature There’s no dangerous reaction but the Substrate Solution will be defective.





10.2 Materials to avoid: Heavy metal salt

There’s no dangerous reaction but the Substrate Solution will be defective. 10.3 Hazardous decomposition products: No data available 11. TOXICOLOGICAL INFORMATION 11.2 Acute effects (toxicity tests)







No CMR effects

Chemical name Wirkdosis Spezies 3,3‘,5,5‘-Tetramethylbenzidin Acute oral toxicity No data

EDTA-di-Natriumsalz Acute oral toxicity LD50 = 2000 mg/kg rat

5-Chlor-2-methyl-4-isothiazolin-3-on Acute oral toxicity LD50 = 3350 mg/kg rat

2-Methyl-4-isothiazolin-3-on Acute oral toxicity LD50 = 550 mg/kg rat

Hydrogen peroxide Acute oral toxicity Akute dermal toxicity

LD50 = 1232 mg/kg LD50 = 3000 mg/kg

rat rabbit





12. Ecological information 12.1 Ecotoxicity:

These data are only related to the preserving agents 5-Chlor-2-methyl-4-isothiazolin-3-on and 2-Methyl-4-isothiazolin-3-on.


No data available 13. DISPOSAL CONSIDERATIONS 13.1 Appropriate disposal / Product:





Official transport designation: Substrate Solution Because product is no dangerous good, no specific codes or labes are

necessary.

Aquatic toxicity Effectdose Exposure time Species Acute fish toxicity LC50=0.19 mg/l No data Trout

Acute daphnia toxicity EC50=0.16 mg/l No data Daphnia magna

Acute algae toxicity EC50=0.018 mg/l No data Selenastrum capricornutum






Labelling (Regulation (EC) No 1272/2008) Hazard components for labelling: - Signal words: not applicable Hazard pictograms: not applicable Hazard statements: not applicable 16. OTHER INFORMATION 16.1 Training instructions: no data 16.3 Data sources: BDI Helpdesk (Germany), ECHA, GESTIS (IFA) 16.4 Further information:



Trade name: Propidium Iodide



1. IDENTIFICATION OF THE PREPARATION AND OF THE

COMPANY/UNDERTAKING

1.1 Identification of the preparation: Identification on the label / trade name: Propidium Iodide Additional identification: 2% Propidium Iodide solution 1.2 Use of the preparation: Propidium Iodide solution is used to stain cells. 1.3 Company/undertaking identification: Bender MedSystems GmbH Campus Vienna Biocenter 2 1030 Vienna, Austria phone: +43 1 796 40 40 100 fax: +43 1 796 40 40 400

E-Mail: [email protected] Information contact: [email protected] National contact: Customer Service 1.4 EMERGENCY TELEPHONE: phone: +43 1 796 40 40 100 (Austria) phone: +1 (888) 999 1371 (USA) These phone numbers are available during office hours.

2. HAZARDS IDENTIFICATION 2.1 Hazards description: Causes irritation of eyes, skin and respiratory system. 2.1.1 Classification:

Regulation (EC) No 1272/2008 [CLP] Hazard classes/Hazard categories Hazard statement Causes irritation on skin Causes irritation on eyes Causes irritation of respiratory system

H315 H319 H335










Hazard- statement

Propidium iodide Dest. ater NaCl HEPES CaCl2

247-081-0 231-791-2 231-598-3 230-907-9 233-140-8

No data No data No data No data 017-013-00-2

25535-16-4 7732-18-5 7647-14-5 7365-45-9 10043-52-4

- - - - -

3.1/ Cat. 4 3.2/ Cat 2 3.3/ Cat 2 - - - - 3.3/ Cat 2

H302,H312,H332,H315 HH319 - - - - H319

Xn;Xi;R36/37/38, R20/21/22 - - - - - Xi; R36

4. FIRST AID MEASURES 4.1 General informations: In case of indisposition contact a doctor, show this datasheet. 4.2 In case of skin contact: Wash affected bodyparts with plenty amount of water. Consult a doctor. 4.3 In case of eye contact: Wash the affected eye with water for a minimum of 10 minutes. In case of indisposition contact an eye-specialist.

67/548/EEC or 1999/45/EC







Consult a doctor.

5. FIRE-FIGHTING MEASURES 5.1 Suitable extinguishing media: Water, dry powder extinguisher, carbondioxid 5.2 Extinguishing media which must not be used for safety reasons: Attention with dry powder extinguisher.



In case of fire, despite flue gas also dangerous, product specific gases might be produced. Because of the very low concentration of this mixture it is very unlikely that oxides will be produced in a hazardous amount. Further data are not known.




Afterwards clean bench with water.





7. HANDLING AND STORAGE 7.1 Handling Advices on safe handling: Protective measures: wear protective clothing Precautions against fire and explosion: Development of explosive atmosphere is not possible 7.2 Storage Technical measures and storage conditions:

Storage in well closed containers Packaging materials: Store in lightproof containers Requirements for storage rooms and vessels: Prevent direct sunlight and heat. Store in well aired storage rooms. Further information on storage conditions: Storage temperature: 2 – 8°C Storage stability: stable Maximal storage periode: see product packaging 8. EXPOSURE CONTROLS / PERSONAL PROTECTION 8.1 Exposure limit values: 8.1.1 Components with occupational exposure limits requiring monitoring:

No data available 8.2 Personal protection equipment: Respiratory protection: not necessary Hand protection: disposable protective gloves Eye protection: not necessary Body protection: lab coat, lab shoes





9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Appearance Physical state: liquid Colour: red Odour: odourless 9.2 Important health, safety and environmental information 9.2.1 Safety relevant data Value Remark_ pH (20 °C): no data Melting point (°C): no data Boiling point (°C): no data Ignition temperature (°C): no data Vapour pressure (°C): no data

Density (g/cm3): no data Water solubility: no data



10.2 Materials to avoid: No data available 10.3 Hazardous decomposition products: No data available











Repeated dose toxicity (sub-acute, sub-chronic, chronic) No data available CMR effects (carcinogenity, mutagenicity and toxicity for reproduction)

No CMR effects 12. Ecological information 12.1 Ecotoxicity: No data available 12.2 Mobility: Known or predicted distribution to environmental compartments:

In case of shifting the pH value there might be some damaging effects for aquatic organism. The mixture must not dispose in the sewage system without pre-treatment.

25535-16-4 Propidium Iodide Effect dose Species

Acute oral toxicity LD50 = 16 mg/kg mouse


Acute inhalative toxicity no data










Official transport designation: Propidium Iodide

Because product is no dangerous good, no specific codes or labels are necessary.


Labelling (Regulation (EC) No 1272/2008) Hazard components for labelling: Propidium Iodide Signal words: Attention Hazard pictograms: GHS07

Hazard statements: H315, H319, H335





Precautionary statements: P261, P351, P338 16. OTHER INFORMATION 16.1 Relevant R-, H- and P-phrases:

H315: Causes skin irritation H319: Causes serious eye irritation H335 Causes irritation to respiratory system R36: Irritating to eyes R37: Irritation to respiratory system R38: Irritating to skin P261: Avoid breathing dust/fume/gas/mist/vapours/spray

P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

16.2 Training instructions: no data 16.4 Data sources: BDI Helpdesk Germany), ECHA, GESTIS (IFA) 16.5 Further information:


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