Measles and Immunity Lab

A peek into the Vaccination & Immunity Module created and produced by the Health and Science Pipeline Initiative (HASPI) in collaboration with Rady

Children’s Hospital, the Jonas Salk Legacy Foundation, the San Diego Rotary, and the

San Diego Foundation.

What is in the Module?• Background Information• Video – A Shot to Save the World• Video – Invisible Threat• Measles and Immunity Lab• Probability Activity• Safety & Effectiveness Research• Campus Survey• Discussion Activity• Vaccination Education Project

Benefits• NGSS & CCSS

aligned resources• Both videos• Lab supplies• Guest speaker• Curriculum support

Requirements• Complete application• Module completion

Spring 2016• Instructor evaluation• Student evaluations

Measles & Immunity LabSplit your table into two lab groups

Each group will need:

one 96-well plate

two plastic pipettes

Share:IgM Test IgG Test

0.1 M Hydrochloric Acid0.1 M Sodium Hydroxide

Each 96-well plate will test 48 subjects • Rows A – D are for the IgM Test (infection antibodies)• Rows E – H are for the IgG Test (memory antibodies)• Wells A1 and E1 are for the same subject

Prepping the Plates – pgs. 3 - 4

Rows E-H

Add a drop of Sodium Hydroxide

to 35 – 45 wells

Positive wells indicate IMMUNITY

The IgG Test is phenolphthalein

Rows A-D

Add a drop of Hydrochloric Acid

to 5 – 10 wells

Positive wells indicate INFECTION

IgM Test is bromothymol blue

Perform the Tests – pg. 8

The IgM Test

Using a plastic pipette add a drop of

IgM Testto every well in

Rows A-D

The IgG Test

Using a plastic pipette add a drop of

IgG Testto every well in

Rows E-H

Results – pg. 9

Thank you for attending!Leave your plates and supplies on the table

Please do not forget to:• Turn in your evaluation

• Pick up and complete the substitute reimbursement form if needed

• Pick up a Measles & Immunity Lab kit

• Complete a Vaccination & Immunity Module Application if interested