1 . NU R FAU ZANA BT ZU LKIFLI (G 1 027668)

2. NO R ‘AID AH BT LU KMAN (G

1 02731 2)

3. WAN S U H AILA BT WAN IS MAIL (G 1 022282)

E D T 5 3 0 3TECHNOLOGY AND

INNOVATION IN EDUCATION( GROUP 12)

TOPIC : MECHANISM OF ENZYME ACTION

C O N TE N T

LEARNING OUTCOME1

2

4

5

6

INTRODUCTION

DEFINITION OF ENZYME

MECHANISM OF ENZYME ACTION

ASSESSMENT

3PRIOR

KNOWLEDGE

LE AR N IN G O U TC O ME

4.10 : Explain the

mechanism of enzyme action

INTR O D U C TIO N

Do you realize the term of COENZYME

on detergent products?

CLICK HERE !!

PR IO R KNO WLE D G E

Maltose

RECALL !!!

Water+ Glucose + Glucose

ENZYME maltase BREAK DOWN maltose into 2 molecules of glucose

Prior knowle d ge

Cloth

Stains

Enzymes

How the stains have been removed from the cloth?

D E F IN ITIO N O F E NZYME

Enzymes are biological catalyst that direct or guide almost all cellular reactions.

ME C H AN IS M O F E NZME

AC TIO N

‘LOCK AND KEY’ HYPOTHESIS

ME C H AN IS M O F E NZME

AC TIO N

Enzyme

Substrate

Enzyme-substrate complex

Product

Product

EXPLANATION EXPLANATION EXPLANATION

ME C H AN IS M O F E NZME

AC TIO N

A specific substrate molecule arrives at the active site of the

enzyme molecule.

ME C H AN IS M O F E NZME

AC TIO N

The substrate molecule binds to the active site to form an

enzyme-substrate complex which is very unstable.

ME C H AN IS M O F E NZME

AC TIO N

Product molecules leave the active site of the enzyme.

The enzyme is free to take another substrate molecule

into its active site to catalyse another reaction.

ME C H AN IS M O F E NZME

AC TIO N

Sucrase

Sucrose

Maltose

Sucrose can bind to active site of sucrase

Maltose CANNOT bind to active site of sucrase

Enzymes have

specific sites called active site which bind to specific substrates

Which substrate can bind to active site of enzyme sucrase?

asse ssm e nt

Question 1 Question 2

Question 3 Question 4

Q ue stion 1

Fill the blank with the correct answer.

2. A specific ______________ molecule arrives

at the active site of the ______________ molecule.

2. The substrate molecule binds to the active site of the

enzyme to form an ______________.

3. The enzyme is free to take another substrate molecule

into its ______________ to catalyse another reaction.

4. ______________ molecules leave the active site of the

enzyme.

substrate

active site

enzyme

Product

enzyme-substrate complex

Q ue stion 2

A. Enzyme reaction is specific

Which of the following shows the characteristic of enzymes?

A. Enzyme reaction is influenced by pH

C. Enzyme reaction is influence by temperature

D. The structure of the enzyme change at the end of the reaction.

Q ue stion 3

Protease Dissolve starch stains on cloth

Amylase

Lipase

Ripening cheese

Dissolve protein stains on cloth

Q ue stion 4

Enzyme is used to wash away the blood stain on a shirt.

Explain how the enzyme acts on the blood stain at a maximum rate.

(3 MARKS)

ANSWER :Blood is made up from proteins. Protease in detergent

will break down protein into amino acids. This reaction occur effectively at 40 ºC which is optimum temperature

for enzyme action.

THANK YOU ….