mechanism of enzyme action

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1. NUR FAUZANA BT ZULKIFLI (G 1027668) 2. NOR ‘AIDAH BT LUKMAN (G 1027312) 3. WAN SUHAILA BT WAN ISMAIL EDT 5303 TECHNOLOGY AND INNOVATION IN EDUCATION ( GROUP 12) TOPIC : MECHANISM OF ENZYME ACTION

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Biology Form 4 (Chapter 4)

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Page 1: Mechanism of enzyme action

1. NUR FAUZANA BT ZULKIFLI (G 1027668)

2. NOR ‘AIDAH BT LUKMAN (G 1027312)

3. WAN SUHAILA BT WAN ISMAIL (G 1022282)

EDT 5303TECHNOLOGY AND

INNOVATION IN EDUCATION( GROUP 12)

TOPIC : MECHANISM OF ENZYME ACTION

Page 2: Mechanism of enzyme action

CONTENTCONTENT

LEARNING OUTCOME1

2

4

5

6

INTRODUCTION

DEFINITION OF ENZYME

MECHANISM OF ENZYME ACTION

ASSESSMENT

3 PRIOR KNOWLEDGE

Page 3: Mechanism of enzyme action

LEARNING OUTCOMELEARNING OUTCOME

4.10 : Explain the

mechanism of enzyme action

Page 4: Mechanism of enzyme action

INTRODUCTIONINTRODUCTION

Do you realize the term of COENZYME

on detergent products?

CLICK HERE !!

Page 5: Mechanism of enzyme action

PRIOR KNOWLEDGEPRIOR KNOWLEDGE

Maltose

RECALL !!!

Water+ Glucose + Glucose

ENZYME maltase BREAK DOWN maltose into 2 molecules of glucose

Page 6: Mechanism of enzyme action

Prior knowledgePrior knowledge

Cloth

Stains

Enzymes

How the stains have been removed from the cloth?

Page 7: Mechanism of enzyme action

DEFINITION OF ENZYMEDEFINITION OF ENZYME

Enzymes are biological catalyst that direct or guide almost all cellular reactions.

Page 8: Mechanism of enzyme action

MECHANISM OF ENZME ACTION

MECHANISM OF ENZME ACTION

‘LOCK AND KEY’ HYPOTHESIS

Page 9: Mechanism of enzyme action

MECHANISM OF ENZME ACTION

MECHANISM OF ENZME ACTION

Enzyme

Substrate

Enzyme-substrate complex

Product

Product

EXPLANATION EXPLANATION EXPLANATION

Page 10: Mechanism of enzyme action

MECHANISM OF ENZME ACTION

MECHANISM OF ENZME ACTION

A specific substrate molecule arrives at the active site of the

enzyme molecule.

Page 11: Mechanism of enzyme action

MECHANISM OF ENZME ACTION

MECHANISM OF ENZME ACTION

The substrate molecule binds to the active site to form an

enzyme-substrate complex which is very unstable.

Page 12: Mechanism of enzyme action

MECHANISM OF ENZME ACTION

MECHANISM OF ENZME ACTION

Product molecules leave the active site of the enzyme.

The enzyme is free to take another substrate molecule

into its active site to catalyse another reaction.

Page 13: Mechanism of enzyme action

MECHANISM OF ENZME ACTION

MECHANISM OF ENZME ACTION

Sucrase

Sucrose

Maltose

Sucrose can bind to active site of sucrase

Maltose CANNOT bind to active site of sucrase

Enzymes have

specific sites

called active site which bind to specific substrates

Which substrate can bind to active site of enzyme sucrase?

Page 14: Mechanism of enzyme action

assessmentassessment

Question 1 Question 2

Question 3 Question 4

Page 15: Mechanism of enzyme action

Question 1Question 1

Fill the blank with the correct answer.

1. A specific ______________ molecule arrives

at the active site of the ______________ molecule.

2. The substrate molecule binds to the active site of the

enzyme to form an ______________.

3. The enzyme is free to take another substrate molecule

into its ______________ to catalyse another reaction.

4. ______________ molecules leave the active site of the

enzyme.

substrate

active site

enzyme

Product

enzyme-substrat

e complex

Page 16: Mechanism of enzyme action

Question 2Question 2

A. Enzyme reaction is specific

Which of the following shows the characteristic of enzymes?

B. Enzyme reaction is influenced by pHC. Enzyme reaction is influence by

temperatureD. The structure of the enzyme change at

the end of the reaction.

Page 17: Mechanism of enzyme action

Question 3Question 3

Protease

Dissolve starch stains

on cloth

Amylase

Lipase

Ripening cheese

Dissolve protein stains

on cloth

Page 18: Mechanism of enzyme action

Question 4Question 4

Enzyme is used to wash away the blood stain on a shirt.

Explain how the enzyme acts on the blood stain at a maximum rate.

(3 MARKS)

ANSWER :Blood is made up from proteins. Protease in detergent will break down protein into amino acids. This reaction

occur effectively at 40 ºC which is optimum temperature for enzyme action.

Page 19: Mechanism of enzyme action

THANK YOU ….