media formulations

7/27/2019 Media Formulations

1/18

Plant Tissue Culture Basal Salts and Media

Product

NumberProduct Description Product Notes

Package

Size

ANDERSON BASAL SALT MIXTUREon a ns e macro- an m cronu r en s as escr e y n erson , .

an ssue u ure es e

Storage Tempo u e n

- Ca er

B144 BANANA AGS BASAL MEDIUM

Contains the macro- and micronutrients, vitamins, and plant growth regulators required

to culture bananas.

Plant Tissue Culture Tested

Storage Temp

Soluble In

2-6 C

ater

1 L

0 L

50 L

usa anana e um: ee - ase an pec c e um ec on

C206 CAPE SUNDEW/ VENUS FLY TRAP

MULTIPLICATION BASAL MEDIUM


or e mu p ca on o carn vorous p an s.

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

ater

1 L

0 L

C216 CAPE SUNDEW/ VENUS FLY TRAP

PRETRANSPLANT BASAL MEDIUM


for the growth of carnivorous plants.

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

ater

1 L

0 L

50 L

C212 CARROT CALLUS INITIATION BASAL MEDIUM


to initiate carrot callus from pith tissue.


Storage Temp

Soluble In

2-6 C

ater

1 L

0 L

50 L

on a ns e macro- an m cronu r en s, v am ns, an p an grow regu a ors requ re

to initiate shoots from carrot callus.


orage emp

o u e n

-

a er

50 L

C287 CHEE & POOL C2d VITIS BASAL MEDIUM

on a ns e macro- an m cronu r en s as escr e y ee oo .

an ssue u ure es e

Storage Temp

o u e n

- C

a er

C167 CHU N6 BASAL MEDIUM w/ VITAMINS

Contains the macro- and micronutrients, and vitamins as described by Chu (1975).


Storage Temp

Soluble In

2-6 C

ater

1 L

0 L

on a ns e macro- an m cronu r en s as escr e y u .


orage emp

o u e n

-

a er

50 L

C149 CHU N6 VITAMIN SOLUTION (1000x)

See Vitamin Section (following the MS-based media section)

Contains macro- and micronutrients as described by Gupta & Durzan (1985).


orage emp

Soluble In

-

ater

0 L

50 L

Our Plant Tissue Culture Tested basal salts and mediahave been formulated according to the referencesc te n t e r respect ve pro uct st ngs. ese asa sa ts

and media are then manufactured and packaged in en-

vironmentally controlled facilities. Our biological testing

ensures t at eac ot pro uces cu ture growt cons stent

with prior lots.

On the following pages are many popular basal salt and

me a ormu as o owe y uras ge an oog- ase

formulations. Vitamin listings and formulations complete

this section. Please contact us with inquiries about larger

package sizes or custom formulations.

nstruct ons or asa sa t, me a, an v tam n preparat on

are provided in the Technical Information Section of this

catalog and on our web site: www.phytotechlab.com.

asal Salt vs Mediu ... our defnition

A Basal Salt Mixture contains macro- and micronutri-

ents, but no vitamins, carbohydrates, or growth regulators.

A Basal Medium contains the macro- and micronutrients,

ut s ncomp ete as t typ ca y ac s an organ c compo-

nent: vitamins, carbohydrates, or growth regulators. A

Medium typically is complete and requires no additional

components except ge ng agent, es re . o e

Basal Salt, Basal Medium, or Medium indicates that one

or more of the components varies in concentration or form

rom t at or g na y pu s e .


2/18

All components expressed in

mg/L

An

dersonBasalSaltMixture

a

nana

asa

e

u

ape

unew

enus

y

rap

Multip.BasalMediu

Cap

eSundew/VenusFlyTrap

reranspan

asa

e

u

a

rro

ausn

aon

asa

e

u

CarrotShootDevelopment

BasalMediu

Chee&PoolC2dVitis

asa

e

u

u

asa

e

u

w/Vitamins

ChuN6BasalSaltMixture

asa

a

xure

Aluminum Chloride6H2O

Ammonium Nitrate

Ammonium Phosphate, Monobasic

mmon um u a e

orc c . . . . . . . .

Calcium Chloride, Anhydrous . . . . . . . .

Calcium Nitrate . .

Cobalt Chloride6H O . . . . . . . .

upr c u a e2

. . . . . . . .

a 2

. . . . . . . .

Ferric Chloride

Ferric Sodium EDTA . .

errous u a e2

. . . . . . . .

agnesum u a e, n y rous . . . . . . . . .

Manganese Chloride2O

Manganese SulfateH2O . . . . . . . .

Molybdic Acid (Sodium Salt)2H2O . . . . . . . .

o y enum r ox e

c e or e2

.

Nickel Sulfate2O

Potassium Chloride

Potassium Iodide . . . . . . . .

o ass um ra e

o ass um osp a e, asc

o ass um osp a e, ono asc

o ass u m u a e

o um ra e

Sodium Phosphate Monobasic .

Sodium Sulfate

nc ra e2

nc u a e2

. . . . . . . .

Adenine Hemisulfate

Glycine (Free Base)

2,4-Dichlorophenoxyacetic Acid

- , me y a y am nopur ne

n o e- -ace c c

ne n .

L(-)Malic Acid

myo-Inositol

co n c c ree c .

yr ox ne .

amneHCI . . .

Grams of powder to prepare 1 liter . . . . . . . . . .

pH0.5 at RT . . . . . . . . . .

NS = No Specication Established rev 11/2005


3/18

3

Plant Tissue Culture Basal Salts and Media (continued)

Product


Package

Size

Contains the macro- and micronutrients as described by Driver & Kuniyuki (1984) and

McGranahan, et al (1987).

orage emp

Soluble In

-

Water

10 L

50 L

DKW BASAL MEDIUMon a ns g ucrose; w ou am ns

on a ns macro- an m cronu r en s as escr e y r ver un yu an

c rana an, e a . .


Storage Tempo u e n - Ca er

DKW BASAL MEDIUM

on a ns g ucrose; w ou v am ns

on a ns macro- an m cronu r en s as escr e y r ver un yu an

McGranahan, et al. (1987).


Storage Temp

o u e n

- C

a er

ERIKSSON VITAMIN SOLUTION (1000x)

ee tamn ecton o ow ng t e - ase me a secton

Contains the macro- and micronutrients as described by Gamborg, et al. (1968).


orage emp

Soluble In

-

Water

10 L

50 LG398 GAMBORG B-5 BASAL MEDIUM

on a ns e macro- an mcronu r en s, an v amns as escr e y am org, e a .

.


Storage Temp

o u e n

- C

a er

G359 GAMBORG (PRL-4-DM) LONG BASAL MEDIUM

Contains the macro- and micronutrients, and vitamins as described by Gamborg, et al.

.

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

a er

1 L

G249 GAMBORG VITAMIN POWDER (1000x)

See Vitamin Section (following the MS-based media section)

x


G371 GRESSHOFF & DOY BASAL MEDIUMContains the macro- and micronutrients as described by Gresshoff & Doy (1974).


Storage TempSoluble In

2-6 CWater

1 L10 L

50 L

on a ns e macro- an m cronu r en s as escr e y e er .

an ssue u ure es e

orage emp

o u e n

-

a er

H396 HELLER/ WHITE MODIFIED BASAL SALT MIXTURE

Contains the macro- and micronutrients as described by Heller (1953) and White

. ou o y enum r ox e

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

a er

1 L

10 L

H353 HOAGLAND MODIFIED BASAL SALT MIXTURE

Contains Ferrous Sulfate

Contains the macro- and micronutrients as described by Hoagland and Arnon (1938).


Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L

e a

See MS-Based Plant-Specic Media (following this section)

K413 KAO & MICHAYLUK BASAL SALT MIXTURE

Contains the macro- and micronutrients as described by Kao & Michayluk (1975).

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

a er

1 L

10 L

Contains the macro- and micronutrients, vitamins, and organic acids as described by

Kao & Michayluk (1975).


orage emp

Soluble In

-

Water

10 L

50 L

x



4/18

4

components

expressed in mg/L

DKWBasalSal

xture

DKWBasal

Mediumw/10g/

Sucrose

DKWBasal

Medium

w/30g/

Sucrose

GamborgBasal

SaltMixture

GamborgB-5

BasalMediu

Gamborg

(PRL-4-DM)Long

BasalMediu

Gresshoff&Do

BasalMediu

HellerBasalSalt

Mixture

Heller/White

ModiedBasal

SaltMixture

HoaglandModied

BasalSaltMixture

Kao&Michayluk

BasalSaltMixture

Kao&Michayluk

ModiedBasal

Medium

COMPONENT D190 D191 D189 G768 G398 G359 G371 H393 H396 H353 K413 K427

Aluminum Chloride6H O .0543 0.03

Ammonium Nitrate 1416 416 1416 1000 600 600

Ammonium Phosphate,Monobasic 115.03

Ammonium Sulfate 134 34 00

Boric Acid 4.8 4.8 .8 3 3 0.3 1 1.24 2.86 3

Calcium Chloride, Anhydrous 112.5 12.5 112.5 113.24 113.24 113.24 6.7 453 453

Calcium Nitrate 1367 367 1367 241.2 300 56.4

Cobalt Chloride6H O .025 .025 .25 0.025 0.025 .025

Cupric Sulfate5H2O .25 0.25 0.25 .025 .025 .25 0.025 .03 0.03 0.08 0.025 .025

Na2EDTA2H

2O 45.4 45.4 45.4 7.26 7.26 186.0 37.25 3.35 37.26 7.26

Ferric Chloride 1

Ferrous Sulfate7H2O 3.8 33.8 33.8 27.8 27.8 139.0 27.85 25 .5 27.85 27.85

Magnesium Sulfate, Anhydrous 361.49 361.49 361.49 122.09 122.09 122.09 17.099 122.1 351.63 240.76 146.55 146.55

Manganese Chloride4H O 1.81

Manganese SulfateH O 3.5 33.5 33.5 10 10 132.0 1 .0758 0.01 10 10

Molybdic Acid (Sodium Salt)2H2O .39 0.39 0.39 0.25 0.25 .25 0.025 .25 0.25

Molybdenum Trioxide .016

Nickel Chloride .03 0.03

Nickel Sulfate6H O 0.005 .005 .005

Potassium Chloride 00 5 750 65 300 300

Potassium Iodide 0.75 0.75 .75 0.8 .01 0.01 .75 0.75

Potassium Nitrate 2500 2500 1000 1000 80 06.6 1900 900

Potassium Phosphate, Monobasic 65 265 265 300 170 170

Potassium Sulfate 1559 559 1559

Sodium Nitrate 600

Sodium Phosphate Monobasic 150 50 0.0 108.75 16.5

Sodium Sulfate 200

inc Nitrate6H2O 17 17 17

inc Sulfate7H2O 2 3 0.3 1 1 0.22 2 2

p-Aminobenzoic Acid 0.2 0.02

L-Arginine (Free Base) 40

L-Ascorbic Acid 0.4 2

Asparagine 40

D-Biotin .00025 0.2 0.01

D-Calcium Pantothenate 0.4 1

Choline Chloride 0.2 1

Citric Acid (Free Acid) Anhydrous 40

Cyancobalamin, Vit B12

0.02

Folic Acid 0.015 .4

Fumaric Acid 40

L-Glutamine 60

Glycine (Free Base) 20 4

L(-)-Maleic Acid

L-Methionine 0.0

myo-Inositol 00 00 10 100

Nicotinamide 1

Nicotinic Acid (Free Acid) 1 0.5 0.1

L-Phenylalanine 20

PyridoxineHCI 1 0.5 0.1 1

Pyruvic Acid 20

Riboavin 0.015 .2

Sucrose 10,000 30,000

ThiamineHCI 10 0.5 1 1

L-Tryptophan 40

itamin A 0.01

itamin D3

0.01

Grams of powder to prepare 1 liter .22 15.22 5.22 3.10 3.21 .64 2.71 .64 1.04 1.63 .65 .9

pH0.5 at RT 4.3 4.0 .1 4.0 4.0 NS 3.9 4.9 4.7 .5 4.0 .4NS = No Specication Established

rev


5/18

5

Product


Package

Size

s me um may e so as u ras ge oog e um w nm a rgancs

(MSMO) by some media suppliers.

Contains the macro- and micronutrients, and vitamins as described by Linsmaier &

Skoog (1965).


orage emp

o u e n

-

a er

50 L

pH Adjusted and Buffered

This medium may be sold as Murashige & Skoog Medium with Minimal Organics

(MSMO) by some media suppliers.


oog .

an ssue u ure es e

orage emp

Soluble In

-

Water

10 L

50 L

L467 LINSMAIER & SKOOG BASAL MEDIUM

w/ 30 g/L SUCROSE & 7 g/L AGAR


Skoog (1965).

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L

L452 LINSMAIER & SKOOG MODIFIED BASAL MEDIUM

w/ 30 g/L SUCROSE & 7 g/L AGAR

pH Adjusted and BufferedContains the macro- and micronutrients, and vitamins as described by Linsmaier &

oog .

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L

L154 LLOYD & McCOWN WOODY PLANT BASAL SALT MIXTURE

Contains the WPM macro- and micronutrients as described by Lloyd & McCown

(1981).


Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L

c w

on a ns e macro- an m cronu r en s, an v am ns as escr e y oy

McCown (1981).


orage emp

o u e n

-

a er

50 L

c

on ans e m c ronu rens escr e y oy c own .

an ssue u ure es e

orage emp

o u e n

-

a er

M419 MG BASAL SALT MIXTURE

Modied Murashige & Skoog/ Gamborg Basal Salt Mixture


Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

See Murashige & Skoog-Based Basal Salts & Media Section (following this section)

N492 NB BASAL MEDIUM

Modied Chu/ Gamborg Basal Medium

on a ns e macronu r en s as escr e y u an e m cronu r en s as

escr e y am org, e a . .

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

a er

1 L

10 L

N613 NITSCH & NITSCH BASAL SALT MIXTURE

Contains the macro- and micronutrients as described by Nitsch & Nitsch (1969).


Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

w

Contains the macro- and micronutrients, and vitamins as described by Nitsch & Nitsch

(1969).


orage emp

Soluble In

-

Water

10 L

50 L

x


N603 NITSCH & NITSCH VITAMIN SOLUTION (1000x)

See Vitamin Section (following the MS-based media section


6/18

All components

expressed in mg/L

Linsmaier&Skoog

asa

e

u

nsmaer

oog

o

easa

e

um

(MSMO)

Linsmaier&SkoogBasal

Mediu

Linsmaier&Skoog

ModiedBasalMedium,

uerep

use

Lloyd&McCownWoody

PlantBasalSaltMixture

Lloyd&McCownWoody

PlantBasalMedium

oy

cown

ooy

PlantMicronutrientMixture

MGBasalSaltMixture

(ModiedMS/Gamborg

BasalSaltMixture)

asa

e

u

sc

scasa

a

Mixture

Nitsch&NitschBasal

e

u

mmon um ra e

Ammonium Sulfate .

Boric Acid . . . . . . . .

Calcium Chloride, Anhydrous . . . . . . . . .

a cum ra e

o a or e2

. . . . . .

Cupric Sulfate5H2O . . . . . . . . . . .

Na2EDTA2H

2O . . . . . . . . . . .

Ferrous Sulfate7H O . . . . . . . . . . .

agnesum u a e, n y rous . . . . . . . . . . .

anganese u a e2

. . . . . . . . . .

Molybdic Acid (Sodium Salt)2H2O . . . . . . . . . . .

Potassium Hydroxide

o ass um o e . . . . . .

o ass um ra e

o ass um osp a e, ono asc .

Potassium Sulfate

Sodium Nitrate .

o um osp a e ono as c .

nc u a e2

. . . . . . . .

gar

D-Biotin .

Folic Acid .

yc ne ree ase

ree c

myo- nos o

Nicotinic Acid (Free Acid) .

PyridoxineHCI . .

ucrose , ,

amne . . . . .

Grams of powder to prepare 1 liter . . . . . . . . . . .

pH0.5 at RT NS . NS NS NS NS . . . NS

NS = No Specication Established rev 11/2005


7/18

7


Product


Package

Size

Contains the macro- and micronutrients, and organic constituents as described by

Lichter (1982); without sucrose.


orage emp

Soluble In

-

Water

10 L

50 L

on a ns e macro- an mcronu r en s as escr e y c o , e a . .

ormua e or e grow o eratopter s r c ar .


orage emp

o u e n

-

a er

Q673 QUOIRIN & LEPOIVRE BASAL SALT MIXTURE

on a ns e macro- an m cronu r en s as escr e y uo r n epo vre an

uo rn, e a . .

an ssue u ure es e

Storage Temp

o u e n

2-6 C

a er

1 L

R756 ROSE MODIFIED INITIATION BASAL MEDIUM

Stage I

Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

R757 ROSE MODIFIED MULTIPLICATION BASAL MEDIUM

Stage IIContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA


Storage Temp

Soluble In

2-6 C

Water

1 L

10 L50 L

age



orage emp

o u e n

-

a er

50 L

S816 SCHENK & HILDEBRANDT BASAL SALT MIXTURE

on ans e macro- an m c ronu rens as escr e y c en an e ran

.

an ssue u ure es e

Storage Temp

o u e n

- C

a er

S811 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM

Contains 10 g/L Sucrose; Without Vitamins

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

a er

1 L

10 L

Contains 10 g/L sucrose, x vitamins, x micro- and x macronutrients.


orage empSoluble In

- Water

10 L

50 L

S806 SCHENK & HILDEBRANDT MODIFIED BASAL SALT MIXTURE

ou ac um

an ssue u ure es e

Storage Temp

o u e n

- C

a er

S813 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM

Contains vitamins and 10g/L sucrose.


Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L

x



8/18

componen s

expressed in mg/Lasa

e

um

arer-

ompson

asc

ern

asa

a

xure

Quoirin&LepoivreBasal

SaltMixture

RoseModiedInitiation

asa

e

u

ose

o

e

u

pcaon

asa

Mediu

RoseModiedRooting

BasalMediu

Schenk&Hildebrandt

asa

a

xure

cen

eran

o

easa

e

u

Schenk&Hildebrandt

ModiedBasalMediu

Schenk&Hildebrandt

ModiedBasalSalt

xure

cen

eran

o

easa

e

u

COMPONENT 479 713 673 756 757 758 S816 S811 S808 S806 813

Ammonium Molybdate .

Ammonium Nitrate .

Ammonium Phosphate,Monobasic

orc c . . . . . . . . . .

Calcium Chloride, Anhydrous . . .

Calcium Nitrate .

o a or e2

. . . . . . . . . .

upr c u a e2

. . . . . . . . . . .

Na EDTA2H O . .

Ferric Sodium EDTA . . . .

Ferrous Sulfate7H O . . .Magnesium Sulfate, Anhydrous . . . . . . . .

anganese u a e2

. . . . . .

o y c c o um a 2

. . . . . . . . . .

o ass um o e . . . . . . . . .

Potassium Nitrate

Potassium Phosphate, Monobasic .

nc u a e2

. . . . . . . . . .

- scor c c

6-Benzylaminopurine (BA) . .

D-Biotin .

r c c ree c n y rous

o c c .L-Glutamine

Glutathion

Glycine (Free Base) . . . .

n o e- -ace c c . .

myo- nos o

- ap a eneace c c .

Nicotinic Acid (Free Acid) . . . . . .

PyridoxineHCI . . . . . .

- er ne

ucrose

amneHCI . . . . . .

Grams of powder to prepare 1 liter . . . . . . . . . . .pH0.5 at RT . . . . . . . . . . .

= o pec ca on s a s e

rev 11/2005


9/18

9


Product


Package

Size


orage emp

Soluble In

-

Water

10 L

50 L

TM4G BASAL MEDIUM

an ssue u ure es e

Storage Tempo u e n - Ca er

T856 TOBACCO MODIFIED CALLUS INITIATION BASAL MEDIUM



Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L

on a ns e a n p ace o errous u a e an so um


orage emp

o u e n

-

a er

50 L

T867 TOBACCO MODIFIED SHOOT & ROOT BASAL MEDIUM


an ssue u ure es e

Storage Temp

Soluble In

2-6 C

a er

1 L

T861 TOBACCO MODIFIED ROOT INITIATION BASAL MEDIUM



Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L

on a ns e macro- an mcronu r en s, an v amns as escr e y o e .

an ssue u ure es e

orage emp

o u e n

-

a er

W865 WESTVACO WV5 BASAL MEDIUM

on a ns e macro- an mcronu r en s, an v amns as escr e y o e a .

an ssue u ure es e

Storage Temp

o u e n

2-6 C

a er

1 L

W898 WHITE BASAL SALT MIXTURE

Contains the macro- and micronutrients as described by White (1963).


Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

Murashige & Skoog Basal Salts and Media following this section.


10/18

0

All components expressed

in mg/Lasa

a

xure

TM4GBasalMediu

oacco

o

eaus

n

aon

asa

e

u

TobaccoModiedShoot

MultiplicationBasal

e

u

oacco

o

e

oo

oo

asa

e

u

TobaccoModiedRoot

InitiationBasalMediu

esvaco

asa

e

u

WestvacoWV5Basal

e

u

e

asa

a

xure

OMPONENT T853 T868 T856 T864 T867 T861 863 865 898

Ammonium Nitrate

mmon um osp a e, ono as c

mmon um u a e

Boric Acid . . . . . . . . .

Calcium Chloride, Anhydrous . . . .

a cum ra e .

o a or e2

. . . . . . . .

Cupric Sulfate2O . . . . . . . . .

Na2EDTA2H

2O . .

Ferric Sodium EDTA . . . . . .

err c u a e .errous u a e . .

Magnesium Sulfate, Anhydrous . . . . .

Manganese SulfateH2O . . . . . . . . .

Molybdenum Trioxide .

o y c c o um a 2

. . . . . . . .

o ass um or e . . .

o ass um o e . . . . . . . . .

Potassium Nitrate . . .

Potassium Phosphate, Monobasic

o um osp a e ono as c .

o um u a e

Zinc Sulfate7H2O . . . . . . . . .D-Biotin .

Casein, Enzymatic Hydrolysate

o c c .

yc ne ree ase . . . . .

Indole-3-acetic Acid . . .

Kinetin . . .

myo- nos o

co n c c ree c . . . . .

yr ox neHCI . . . . .

ThiamineHCI . . . . . . .

Grams of powder to prepare 1 liter . . . . . . . .

p . a . . . .= o pec ca on s a s e rev


11/18

1

Murashige & Skoog-Based Basal SaltsProduct


Package

Size

Contains the macro- and micronutrients as described by Murashige & Skoog (1962).


See Product No. M519 for Murashige & Skoog Basal Medium w/ Vitamins

orage emp

Soluble In

-

Water

0 L

50 L

on a ns e a


orage empo u e n

- a er

50 L

M502 MURASHIGE & SKOOG MACRONUTRIENT SALT BASE

Contains the macronutrients as described by Murashige & Skoog (1962).

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

a er

1 L

x

Contains the macronutrients as described by Murashige & Skoog (1962).


orage emp

Soluble In

-

Water

0 L

50 L

on a ns e m cronu r en s as escr e y uras ge oog .

an ssue u ure es e

orage emp

o u e n

-

a er

M529 MURASHIGE & SKOOG MICRONUTRIENT STOCK SOLUTION (10x)

Contains the micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested

Storage Temp

Soluble In

2-6 C

Water

1 L

0 L

Contains x macro- and x micronutrients as described by Murashige & Skoog

(1962).


orage emp

Soluble In

-

Water

0 L

50 L

on a ns x mmon um ra e an x o ass um ra e, w e rema n ng macro-

an mcronu r en s as escr e y uras ge oog .


orage emp

o u e n

-

a er

M290 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE

Contains x Ammonium Nitrate, x Potassium Nitrate, and x Calcium Chloride, with

e rema n ng macro- an m cronu r en s as escr e y uras ge oog .

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

a er

1 L

M571 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE

Without Ammonium Nitrate. Contains the remaining macro- and micronutrients as

described by Murashige & Skoog (1962).

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

Water

1 L

0 L

50 L

Without Nitrogen. Contains the macro- and micronutrients as described by Murashige

& Skoog (1962), modied by eliminating NH4NO

3and KNO .


orage emp

Soluble In

-

Water

0 L

50 L

ou rogen, osp orous, an o ass um

Contains the remaining macro- and micronutrients as described by Murashige & Skoog

(1962).


orage emp

o u e n

-

a er

50 L


12/18

2

componen s

expresse n mg

uras

ge

oog

asa

a

xure

M

SModiedBasalSalt

Mixturew/FeNaEDTA

MS

MacronutrientSaltBase

acronuren

oc

ouon

x

cronuren

aase

M

SMicronutrientStock

Solution(10x)

o

easa

a

xure

(

1/2xMicros&Macros)

M

SModiedBasalSalt

Mixture(1/2xNitrates)

M

SModiedBasalSalt

Mixture

o

easa

a

Mixture(NoNHNO3)

o

easa

a

xure

o

rogen

M

SModiedBasalSalt

xure

o

,,or

COMPONENT M524 M499 M502 M654 M554 M529 M153 M561 M290 M571 M531 M407

mmon um ra e

orc c . . . . . . . . .


Cobalt Chloride6H O . . . . . . . . . .

upr c u a e2

. . . . . . . . . .

a2

2O . . . . . . . .

Ferric Sodium EDTA .

Ferrous Sulfate7H O . . . . . . . .

agnesum u a e, n y rous . . . . . . . .

Manganese SulfateH O . . . . . . . . .

o y c c o um

a 2

. . . . . . . . . .

Potassium Iodide . . . . . . . . . .

o ass um ra e

Potassium Phosphate,

Monobasic

Zinc Sulfate2O . . . . . . . . .

Grams of powder to prepare

er

. . . . . . . . . .

pH0.5 at RT . NS NS . . . NS . NS NS . .

NS = No Specication Established Rev. 11/2005


13/18

3

Murashige & Skoog-Based MediaProduct


Package

Size

w

e macro- an m cronu r en s, an v am ns as escr e y uras ge oog

(1962).


Contains the basal salts of Product No. M524

orage emp

o u e n

-

a er

50 L

ou o ass um osp a e; con a ns e a n p ace o errous u a e an

Disodium EDTA. Contains the remaining macro- and micronutrients, and modied vitamins

as described by Murashige & Skoog (1962). Also contains (mg/L): 300 Sodium Phosphate

Monobasic, 150 Adenine Hemisulfate, and 1000 Casein Hydrolysate.


orage emp

o u e n

-

a er

50 L

w

e macro- an m cronu r en s as escr e y uras ge oog , an

vitamins as described by Gamborg, et al. (1966).


orage emp

o u e n

-

a er

50 L

on a ns e macro- an m cronu r en s as escr e y uras ge oog ;

mo e w e a on o mg : . an .


orage emp

o u e n

-

a er

M701 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM

This medium was formerly listed as M506.

s me um may e so as uras ge egona u p ca on e um y some me a

supp ers. on a ns e macro- an m cronu r en s as escr e y uras ge oog

; mo e w e a on o mg : an en ne em su a e.


Storage Temp

Soluble In

2-6 C

a er

1 L

M702 MURASHIGE & SKOOG MODIFIED MEDIUM

Contains the macro- and micronutrients as described by Murashige & Skoog (1962);

mo e w e a on o mg : an en ne em su a e, an .

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

a er

1 L

M535 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM

Without Nicotinic Acid, PyridoxineHCl, and Glycine

Contains the macro- and micronutrients as described by Murashige & Skoog (1962);

mo e w e a on o mg . am ne , myo- nos o , an en ne

em su a e.an ssue u ure es e

Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

MURASHIGE MEDIUM WITH MINIMAL ORGANICS (MSMO)

See L689 & L477 Linsmaier & Skoog Basal Medium

on a ns e macro- an mcronu r en s, an v am ns as escr e y uras ge oog

(1962); modied with the addition of (mg/L): 2.0 2iP, 80 Adenine Hemisulfate, and 170

Sodium Phosphate.


orage emp

o u e n

-

a er

50 L

s me um may e so as u ras ge oog oo u p ca on e um y some

me a supp ers. on a ns e macro- an m cronu r en s as escr e y uras ge

Skoog (1962); modied with the addition of (mg/L): 1.0 Kinetin, 80 Adenine Hemisulfate,

and 148 Sodium Phosphate Monobasic.


orage emp

o u e n

-

a er

s me um s so as uras ge oo p oo ng e um y some me a supp ers.

Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and

vitamins as described by Linsmaier & Skoog (1965); modied with the addition of (mg/L):

0.3 IAA, 1.0 Kinetin, and FeNaEDTA.


orage emp

o u e n

-

a er

50 L

s me um s so as oo u p ca on e um y some me a supp ers.

Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and

vitamins as described by Linsmaier & Skoog (1965); modied with the addition of (mg/L):

170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 30 2iP, and 0.3 IAA.


orage emp

o u e n

-

a er

50 L

ee tamn ect on o owng t s sect on


14/18

4

All components

expressed in mg/L

uras

ge

oog

asa

e

u

o

easa

e

um

(w/outKH2PO4)

MSModiedBasalMediu

w/GamborgVitamins

o

ee

umw

o

easa

e

um

w/2iP

MS

ModiedMediumw/2iP

&IAA

o

easa

e

u

uras

ge

o

e

Mu

ltiplicationBasalMediu

w/2iP

M

SModiedMultiplication

Mediumw/Kinetin

MurashigeModied

u

pcaon

asa

e

um

wnen

M

urashigeModiedShoot

Mu

ltiplicationBasalMediu

OMPONENT 519 M541 404 401 M701 702 535 M536 555 527 491

mmon um ra e

orc c . . . . . . . . . . .


Cobalt Chloride6H O . . . . . . . . . . .

Cupric Sulfate5H O . . . . . . . . . . .

a2

2

. . . . . . .

err c o um . . . .

Ferrous Sulfate7H2O . . . . . . .

Magnesium Sulfate, Anhydrous . . . . . . . .

Manganese SulfateH O . . . . . . . . . . .

o y c c o um a 2

. . . . . . . . . . .

o ass um o e . . . . . . . . . . .

Potassium Nitrate


o um osp a e ono as c

nc u a e . . . . . . . . . . .

Adenine Hemisulfate

6-Benzylaminopurine (BA)


yc ne ree ase .

6-, Dimethylallylaminopurine (2iP)

n o e- -ace c c . . .Indole-3-butyric Acid

Kinetin

myo-Inositol

- ap a eneace c c . .

co n c c ree c . . .

PyridoxineHCI . . .

Sucrose

am ne . . . . . . . . . .

rams o pow er o prepare er . . . . . . . . . . .

p . a . . . . . . . . .

NS = No Specication Established Rev 11/2005


15/18

5

MS-Based Plant-Specifc Media

Product


Package

Size

Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the

remaining macro- and micronutrients as described by Murashige & Skoog (1962).


orage emp

o u e n

-

a er

50 L




orage emp

o u e n

-

a er

50 L

ra ops s u ure e um

o e w e a on o mg : . , - , an . ne n


orage emp

o u e n

-

a er

M506 MURASHIGE BEGONIA MULTIPLICATION MEDIUM

ee ro uc o . 1 uras ge oog o e e um same ormua on




orage emp

Soluble In

-

Water

10 L

50 L

on a ns e a n p ace o errous u a e an so um . on a ns e

rema n ng macro- an m cronu r en s as escr e y uras ge oog .


orage emp

o u e n

-

a er

M510 MURASHIGE MODIFIED GERBERA PRETRANSPLANT BASAL MEDIUM



an ssue u ure es e

Storage Temp

o u e n

2-6 C

a er

1 L

H435 HOSTA INITIATION/ MULTIPLICATION MEDIUM

Stage I/II Medium

Modied Murashige & Skoog (1962) with (mg/L): 2.0 BA, 160 Adenine Hemisulfate, 500

ase n y roysa e, an .

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

H436 HOSTA MULTIPLICATION MEDIUM

Stage II Medium

Modied Murashige & Skoog (1962) with (mg/L): 0.1 BA, 160 Adenine Hemisulfate, 500

ase n y roysa e, an .

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L

H437 HOSTA ROOTING MEDIUM

Stage III Medium

Modied Murashige & Skoog (1962) with (mg/L): 0.1 BA, 500 Casein Hydrolysate, and 0.5

an ssue u ure es e

Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L


16/18

6

All components

expressed in mg/L

Murashig

eModiedAfricanViolet/

GloxiniaM

ultiplicationBasalMediu

urasge

o

ercan

oe

GloxiniaPretransplantBasalMediu

o

ee

u

ra

opss

Mura

shigeModiedFern

up

caon

asa

e

u

MurashigeModiedGerbera

MultiplicationBasalMediu

uras

ge

o

eerera

reranspan

asa

e

u

HostaInitiation/Multiplication

e

u

Hosta

MultiplicationMediu

Hos

taRootingMediu

urasge

oog

asa

e

um

us

eormua

eerence

COMPONENT 517 518 550 508 M509 510 435 436 437 519

Ammonium Nitrate

Boric Acid . . . . . . . . . .

a c u m or e, n y rous . . . . .

o a or e2

. . . . . . . . . .

Cupric Sulfate2O . . . . . . . . . .

Na2EDTA2H

2O . . . . .

Ferric Sodium EDTA . . . . .

errous u a e2

. . . . .

agnesum u a e, n y rous . . . . .

Manganese SulfateH2O . . . . . . . . . .

Molybdic Acid (Sodium Salt)2H2O . . . . . . . . . .

o ass um o e . . . . . . . . . .

o ass um ra e

o ass um osp a e, ono asc

Sodium Phosphate Monobasic

Zinc Sulfate7H2O . . . . . . . . . .

enne em su a e

gar

6-Benzylaminopurine (BA) . . .


Glycine (Free Base) . . .

, - c orop enoxyace c c .

n o e- -ace c c . . .

n o e- - u yr c c

Kinetin . . .

MES (Free Acid)

myo- nos o

- ap a eneace c c . . . .

Nicotinic Acid (Free Acid) . .

Peptone from Meat

PyridoxineHCI . . . .

ucrose

am ne . . . . . . .

L-Tyrosine

Grams of powder to prepare 1 liter . . . . . . . . . .

pH0.5 at RT . . . . . . . . .

= o pec ca on s a s e ev


17/18

7

MS-Based Plant-Specifc Media (continued)

Product


Package

Size




orage emp

o u e n

-

a er

M512 MURASHIGE MODIFIED KALANCHOE PRETRANSPLANT BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the


an ssue u ure es e

Storage TempSoluble In

2-6 Ca er

1 L

M513 MURASHIGE MODIFIED LILY MULTIPLICATION BASAL MEDIUM




Storage Temp

Soluble In

2-6 C

Water

1 L

10 L

50 L

IITA Formulation as described by Vuylsteke (1998).


orage emp

Soluble In

-

Water

10 L

50 L




orage emp

Soluble In

-

Water

10 L

50 L

All components

expressed in mg/L uras

ge

o

e

aancoe

Mult-

plication

Basal

e

u

Murashige

Modied

aancoe

re-

ranspan

Basal

Mediu

uras

ge

o

e

LilyMulti-

plication

Basal

e

u

Musa

(Banana)

u

-

pcaon

e

uMS

o

e

oao

asa

Mediu

OMPONENT 511 512 M513 462 516

Ammonium Nitrate

Boric Acid . . . . .

Calcium Chloride, Anhydrous .

Cobalt Chloride6H . . . . .

Cupric Sulfate5H O . . . . .

a O .

erric Sodium EDTA . . . .

errous Sulfate7H2O .

Magnesium Sulfate, Anhydrous .

Manganese SulfateH2

. . . . .

Molybdic Acid (Sodium Salt)2H2O . . . . .

Potassium Iodide . . . . .

Potassium Nitrate


Zinc Sulfate7H2O . . . . .

L-Ascorbic Acid

6-Benzylaminopurine (BA) .

Gelrite

D-Glucose 2.0

Glycine (Free Base) 2.0 2.0

6- , -Dimethylallylaminopurine (2iP) .0

ndole-3-acetic Acid .0 .175

Kinetin .04

myo-Inositol 100 100 100 100

-Naphthaleneacetic Acid 0.03

Nicotinic Acid (Free Acid) 0.5 .5

PyridoxineHCI 0.5 .5

Sucrose 30,000

ThiamineHCI .4 .4 0.4 0.4 .4

Grams of powder to prepare 1 liter 4.41 .41 4.4 6.36 .41

pH0.5 at RT 4.8 4.8 4.8 4.3 S

NS = No Specication Established


18/18

8

Vitamins

Product


Package

Size

C149 CHU N6 VITAMIN SOLUTION (1000x)

on a ns e v am ns as escr e y u .

an ssue u ure es e

Storage Temp

o u e n

- C

a er

m

E330 ERIKSSON VITAMIN SOLUTION (1000x)

Contains the vitamins as described by Eriksson (1965).Plant Tissue Culture Tested

Storage Temp

Soluble In

2-6 C

Water

100 mL

x

on a ns e v am ns as escr e y am org, e a . .


orage emp

o u e n

-

a er

m

G219 GAMBORG VITAMIN SOLUTION (1000x)

on a ns e v am ns as escr e y am org, e a . .

an ssue u ure es e

Storage Temp

o u e n

- C

a er

m

K421 KAO & MICHAYLUK VITAMIN SOLUTION (100x)

Contains the vitamins described by Kao & Michayluk (1975).


Storage Temp

Soluble In

2-6 C

Water

100 mL

500 mL

x

on a ns e v am ns as escr e y uras ge oog .


orage emp

o u e n

-

a er

m

m

MURASHIGE & SKOOG VITAMIN SOLUTION (1000x)

on a ns e v am ns as escr e y uras ge oog .

an ssue u ure es e

Storage Temp

o u e n

- C

a er

m

M547 MURASHIGE & SKOOG MODIFIED VITAMIN POWDER (1000x)

Murashige & Skoog (1962) vitamins modied with additional ThiamineHCl.


100 mL

250 mL

x

Murashige & Skoog (1962) vitamins modied with additional ThiamineHCl.


orage emp

Soluble In

-

Water

m

NITSCH & NITSCH VITAMIN POWDER (1000x)

on a ns e v am ns as escr e y sc sc .

an ssue u ure es e

Storage Temp

o u e n

- C

a er

m

m

N603 NITSCH & NITSCH VITAMIN SOLUTION (1000x)

Contains the vitamins as described by Nitsch & Nitsch (1969).


Storage Temp

Soluble In

2-6 C

Water

100 mL

x

Contains the vitamins as described by Schenk & Hildebrandt (1972).


orage emp

Soluble In

-

Water

m

1 L

All components

expressed in mg/LChuN6Vitamin

Solution(1000x)

ErikssonVitamin

Solution(1000x)

GamborgVitamin

Powder(1000x)

GamborgVitamin

Solution(1000x)

Kao&Michayluk

VitaminSolution

(100x)

MSVitamin

Powder(1000x)

MSVitamin

Solution(1000x)

MSModied

VitaminPowder

(1000x)

MSModied

VitaminSolution

(1000x)

Nitsch&Nitsch

VitaminPowder

(1000x)

Nitsch&Nitsch

VitaminSolution

(1000x)

Schenk

Hildebrandt

VitaminPowder

(100x)

p- m no enzo c c .

L-Ascorbic Acid

D-Biotin .

- a c um an o ena e

Choline Chloride

yanco a am n,12

.

o c c . .

Glycine (Free Base)

myo-Inositol

co nam e

co n c c ree c

yr ox ne HCl .

Riboavin .

Thiamine HCI

media formulations

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