Page 2
A culture medium
• Water
• Carbon
• Nitrogen,sulphur,phosphorus,oxygen,hydrogen and
• Metal ions, vitamins
Page 5
Liquid cuture media
• Basal media: Nutrient broth
-Beef extract
-peptone
-NaCl
-Water
Page 7
Enriched media
• Basal media
• Supplemented with serum, blood or extracts of plant
Page 8
Differential media
• Blood agar(5%sheep blood+trypticase soy agar)
• Streptococcus pyogenes(Beta-hemolysis)
Page 12
Selective media
• MacConcey agar
• Endo agar
• Eosin methylene blue(EMB) agar
Page 13
MacConcey Agar
• Selective for gram-negative bacteria
• Differential for lactose fermentation
Page 17
Eosin methylene blue(EMB) agar (E.coli)
Page 40
Candle jar:capneic incubation
Page 42
pH of media: neutral
ph of fungal media: slightly acidic
pH of vibrio media: alcaline