membranes 5357 schlesinger lecture title biology 5357 ... · membranes 5357 schlesinger lecture...
TRANSCRIPT
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
Biology 5357: MembranesIntroduction, Plausibility, Reality and
Some Physics
Paul H. Schlesinger MD, PhD
Department of Cell Biology and [email protected]
362-2223
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
Lipid Crystallography
They represent a low energy andpresumably stable molecular state while
providing atomic scale structuralinformation.
I Membranes are“fluid”
I Membranes arehydrated
I Membranes areanisotropic
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
Lipid Crystallography
Fatty Acids e.g. Potassium Palmitate
I Maxmize favorablepacking
I Headgroups are hydrogenbonded
I Chains are anti-, torsionangle < 30
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
Acylglycerol Structures
1-acyl chain two acyl chains
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
Acylglycerol Structures
Trans–LeafletH-Bonding
No Trans LeafletH–bonds
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
Acylglycerol Structures
Glycerol ⊥ Glycerol ‖
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
TriacylglycerolI Nonlamellar LipidI Relatively weak
headgroup polarityI Lateral packing in a
single layerI Polar region in the
single layerJensen & Mabis (1966)
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
Crystal Structure Analysis
Φ = cos−1(n∑
S )
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
GlycerophosphateStructures
DiLauryl-Glycerol-PhosphateHitchcock (1974)
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
GlycerophosphateStructures
Lysophos-phatidyl-ethanolamine
(n∑
S ) = 0.538
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
GlycerophosphateStructures
3-Laurylpropanediol-1-phosphocholine
Structure basedthickness ofbilayer
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
Membrane LeafletsStereochemical Leaflet Segregation
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructuresGlycerophosphateCrystals
Stereochemistry of lipidstructures
End Marker
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
A. Cornish-Bowden and M. Luz Cardenas.Life before luca.Journal of Theoretical Biology, 2017.
C von Nageli.Wachstumsgeschichte von pterothamnionplumula und floccosum.ln: C. von Nageli & C. Cramer (eds.),Pjlanzenphysiologische Untersuchungen.Friedrich Schulthess: Zurich, pages 54–68,1855.claimed to observe separating membraneformation during cell division.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
Die Lehre von der Pflanzenzelle Hofmeister.Handb. d. physiol.Botanik. I. Leipzig, 1867.
John E. Heuser.The origins and evolution of freeze-etch electronmicroscopy.Journal of Electron Microscopy, 60(suppl1):S3–S29, August 2011.
R. Milo, R. Phillips, and N. Orme.Cell Biology by the Numbers.Garland Science, 2016.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
Henri Dutrochet.Nouvelles recherches sur l’endosmose etl’exosmose... de l’irritabilite vegetale...JB Bailliere, 1828.
Jacobus H. van ’t Hoff, 1901.
M. Perouansky.The overton in MeyerOverton: a biographicalsketch commemorating the 150th anniversary ofcharles ernest overton’s birth.British Journal of Anaesthesia, 114(4):537–541,April 2015.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
T Heimburg.Thermal Biophysics of Membranes, chapterAnesthesia, pages 289–300.Wiley-VHC, Weinheim, 2007.
Ellyn Gray, Joshua Karslake, Benjamin B.Machta, and Sarah L. Veatch.Liquid general anesthetics lower criticaltemperatures in plasma membrane vesicles.Biophysical Journal, 105(12):2751 – 2759, 2013.
Thomas Heimburg.Nerves and anesthesia: A physics perspectiveon medicine.arXiv, September 2014.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
Kaare Grasboll, Henrike Sasse-Middelhoff, andThomas Heimburg.The thermodynamics of general and localanesthesia.Biophysical Journal, 106(10):2143 – 2156, 2014.
Linus Pauling.A molecular theory of general anesthesia.Science, 134:15–21, 1961.The hydrate-microcrystal theory of anesthesiaby non-hydrogen-bonding agents differs frommost earlier theories in that it involves primarilythe interaction of the molecules of the anestheticagent with water molecules in the brain, ratherthan with molecules of lipid.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
Leonard L Firestone, Andrew S Janoff, andKeith W Miller.Lipid-dependent differential effects ofstereoisomers of anesthetic alcohols.Biochimica et Biophysica Acta (BBA) -Biomembranes, 898(1):90 – 96, 1987.
E. Gorter and F. Grendel.On the bimolecular layers of lipoids on thechromocytes of the blood.J. Exp. Med., 41:439–443, 1925.
A. Pockel.Surface tension.Nature, 43:437–439, 1891.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
K. A. Dill and S. Bromberg.Molecular Driving Forces, chapter Adsorption,Binding & Catalysis, pages 515–532.Garland Science, 2002.
Tao Ni, Antreas C. Kalli, Fiona B. Naughton,Luke A. Yates, Omar Naneh, Mirijam Kozorog,Gregor Anderluh, Mark S.P. Sansom, andRobert J.C. Gilbert.Structure and lipid-binding properties of thekindlin-3 pleckstrin homology domain.Biochemical Journal, 474(4):539–556, 2017.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
Hyunju Cho, Ming Wu, Betul Bilgin, S. PatrickWalton, and Christina Chan.Latest developments in experimental andcomputational approaches to characterizeproteinlipid interactions.PROTEOMICS, 12(22):3273–3285, 2012.
H. Hauser and G. Poupart.The Structure of Biological Membranes, pages1–53.CRC Press, 2 edition, 2005.
T Heimburg.Thermal Biophysics of Membranes, chapterIntroduction to Thermodynamics, pages 41–61.Wiley-VHC, Weinheim, 2007.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
K.E. van Holde, W.C. Johnson, and P.S. Ho.Principles of Physical Biochemistry, chapterMacromolecules in Solution: Thermodynamicsand Equilibrium, pages 528–563.Prentice Hall, Upper Saddle River, New JerseyUSA, 1998.
S Blundell and K Blundell.Concepts in Thermal Physics, chapter 28.Phase transitions.Oxford University Press, 2009.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
Emiliano Brini, Christopher J. Fennell, MariviFernandez-Serra, Barbara Hribar-Lee, MihaLuksic, and Ken A. Dill.How water’s properties are encoded in itsmolecular structure and energies.Chemical Reviews, 0(0):null, 0.PMID: 28949513.
T. Heimburg.Thermal Biophysics of Membranes, chapterPhase Diagrams, pages 99–121.Wiley-VHC, Weinheim, 2007.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
W. Helfrich.Elastic properties of lipid bilayers: theory andpossible experiments.Z. Naturforsch., 28:693–703, 1973.
H. A. Pinnow and W. Helfrich.Effect of thermal undulations on the bendingelasticity and spontaneous curvature of fluidmembranes.The European Physical Journal E,3(2):149–157, 2000.
Membranes 5357
Schlesinger
Lecture Title
The Cell Boundry
Membranes II.Composition andStructure
Lipid Structures
Steroids
CrystalStructures
End Marker
Michael F. Brown.Curvature forces in membrane Lipid-Proteininteractions.Biochemistry, 51(49):9782–9795, December2012.
S Blundell and K Blundell.Concepts in Thermal Physics, chapter 17. RodsBubbles and Magnets, pages 191–202.Oxford University Press, 2009.