metabolic engineering of alkaloids production in plant
DESCRIPTION
Metabolic engineering of alkaloids production in plant. Juan Pan Department of Plant Pathology. outline. alkaloids biosynthesis in plant metabolic engineering summary. Alkaloids. N-containing small molecule Amino acid derivative S econdary metabolites Defense in plant - PowerPoint PPT PresentationTRANSCRIPT
METABOLIC ENGINEERING OF ALKALOIDS PRODUCTION IN PLANT
Juan PanDepartment of Plant Pathology
OUTLINE
• alkaloids biosynthesis in plant• metabolic engineering• summary
ALKALOIDS- N-containing small molecule - Amino acid derivative- Secondary metabolites- Defense in plant- Pharmaceutical uses
Alkaloid biosynthesisIn plant- monoterpene indole alkaloid (MIA)
Annu. Rev. Plant Biol. 2008. 59:735-69
MIA biosynthesis scheme
Alkaloid biosynthesis in plant- benzylisoquinoline alkaloids (BIAs)
tyrosine (S)-norcoclaurine
Nature chemical biology, 2009, 5 (5): 292-300
dopamine
4-hydroxyphenylacetaldehyde
(S)-reticuline
Alkaloid biosynthesis in plant- tropane alkaloids
Alkaloid biosynthesis in plant- purine alkaloids
Metabolic engineering of alkaloid biosynthesis
- Overexpressing rate- limiting genes- Gene silencing to block pathway- Regulatory element
H6H
PMT and H6H overexpression increased scopolamine production
Proc Natl Acad Sci USA 101: 6786-6791
tyrosine (S)-norcoclaurine
dopamine
4-hydroxyphenylacetaldehyde
(S)-reticuline
COR
Silencing COR blocked morphine biosynthetic pathway
Nat Biotechnol 22: 1559-1566
Nat Biotechnol 22: 1559-1566
Plant Biotechnology Journal (2008) 6, pp. 160–175
Exogenous regulatory factors increased alkaloids production in poppy
SUMMARY
- Overexpression of rate-limiting enzyme for higher alkaloid production- Gene silencing to block certain pathway- Manipulating regulatory elements to increase alkaloid production- Combination of several methods