metathesis polymerization (romp) in an emulsion system microwave-assisted … · 2017-09-12 ·...
TRANSCRIPT
![Page 1: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/1.jpg)
Electronic Supplementary Information (ESI)
Microwave-assisted synthesis of glycopolymers by ring-opening
metathesis polymerization (ROMP) in an emulsion system
Fei Fan,a,c Chao Cai,*a,b,c Lei Gao,a,c Jun Li,a,c Ping Zhang,a,c Guoyun Li,a,b,c Chunxia Li,a,b,c and Guangli Yu*a,b,c
a Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. E-mail: [email protected] (CC),
[email protected] (GY).b Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine
Science and Technology, Qingdao 266003, China.c Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of
Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Electronic Supplementary Material (ESI) for Polymer Chemistry.This journal is © The Royal Society of Chemistry 2017
![Page 2: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/2.jpg)
Table of Contents:
1. NMR data for glycomonomers.
2. The features of polymerization for glycomonomer 5 with H-G 2nd as catalyst.
3. The specific refractive index increment (dn/dc) determination.
4. Synthetic routes of homoglycopolymers and mult-block glycopolymers.
5. NMR spectra.
6. The size-exclusion chromatography (SEC) spectra.
7. Surface Plasmon Resonance measurements.
![Page 3: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/3.jpg)
1
1. NMR data for glycomonomers.Table S1. 1H and 13C NMR data for glycomonomers (5, 10, 14).
NN
OHOHO
HO
O
OH
NN
O
O
NN
N
O
OHOH
OH
O N
O
ON
O
O
N
NN
OHO
OH
HOOH
O1
1
2
2
4
435
67
891011
12 1314
1
1
2
2
4
435
67
8
1
1
2
2
4
4
35
67
899
10
10
11
11
12
12
13
13
14
14
(5) (10) (14)
16 15
15 16 15
15
16 15
15
5 10 14Atom
δH nH m. J[Hz] δC δH nH m. J[Hz] δC δH nH m. J[Hz] δC
137.59 137.611 6.32 2 s
137.586.25 2 s 137.90 6.33 2 s
137.55
45.072 3.19 2 s 45.06 3.21 2 s 45.25 3.19 2 d 10.3
45.06
1.44 1 d 9.8 1.43 1 d 8.8 1.45 1 d 9.83
1.23 1 d 9.842.20
1.23 1 d 9.242.86
1.28 1 d 9.842.21
47.684 2.76 2 d 0.8 47.66 2.72 2 s 47.91 2.77 2 s
47.67
5 4.73 2 s 32.92 4.71 2 s 33.50 4.73 2 t 4.1 32.95
6 8.04 1 s 124.59 7.79 1 s 124.23 8.02 1 s 124.40
7 4.61 2 t 5.1 50.22 4.54 2 t 17.8 50.00 4.61 2 m 49.85
4.20 1 m 4.01 1 s 4.01 1 ddd11.7, 7.7,
4.38
3.98 1 dt11.0,
5.0
67.60
3.82 1 d 10.9
66.49
3.79 1 dt 10.7, 4.0
65.66
9 4.28 1 d 7.8 103.17 4.76 1 s 99.86 4.73 1 t 4.1 98.95
10 3.16 1 dd 9.0, 7.9 73.55 3.82 1 d 10.9 70.44 3.71 1 dd 10.0, 3.7 68.38
11 3.31 1 dt 3.1, 1.6 76.52 3.66 1 d 8.5 71.28 3.61 1 m 70.10
12 3.27 1 m 76.67b 3.07 1 d 8.1 72.76 3.59 1 m 71.94
13 3.27 1 m 70.14b 3.76 1 d 9.4 65.49 3.29 1 d 6.6 66.28
3.87 1 d 11.0 3.76 1 d 9.4
143.66 1 dd
11.9,
5.3
61.313.66 1 d 8.5
61.06 1.08 3 d 6.6 15.16
177.86 177.81415 177.86
177.83 177.805
16 141.85 141.91 142.01
b might be interchanged
![Page 4: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/4.jpg)
2
2. The features of polymerization for glycopmonomer 5 with H-G 2nd as catalyst.
Figure S1. (a) Reaction time of polymerization for [M]/[C] is 20 with H-G 2nd as catalyst under various reaction
temperature (table 1, entries 6-11). (b) Monomer conversion of polymerization for various [M]/[C] ratio with H-G
2nd as catalyst under the reaction temperature 75 °C (table 1, entries 10, 11, 14-17). The black plot represents
standard microwave heating, the red plot represents conventionally heating. (c) Different molecular weight (red)
and polydispersity index (PDI, blue) polymerization with H-G 2nd as catalyst under different ratio of aqueous
phase to organic phase.
![Page 5: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/5.jpg)
3
3. The specific refractive index increment (dn/dc) determination.
Figure S2. The standard curve for the specific refractive index increment (dn/dc) determination.
![Page 6: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/6.jpg)
4
4. Synthetic routes of homoglycopolymers and mult-block glycopolymers.
N
O
O
NN
Ph
n
N
N
O
O
NN
m
OHOHO
HO
O
OH
N
OHOHO
OH
HOO
N
O
O
NN
Ph
n
N
N
O
O
NN
m
N
O
OHOH
OH
O
OHOHO
OH
HOO
1) 2-Azidoethanol,Dowex50W-X8 (H+),80 °C
OHOHO
HO OH
OH
O
OHOH
OH
OH
NN
OHOHO
HO
O
OH
N
NN
N
O
OHOH
OH
O N
O
O
DTAB, H-G 2nd, MW 75 °C
bis-Tris buffer/DCE=2/1p-Glu
10, DTAB, H-G 2nd, MW 75 °C
bis-Tris buffer/DCE=2/1
DTAB, H-G 2nd, MW 75 °C
bis-Tris buffer/DCE=2/1p-Glu
14, DTAB, H-G 2nd, MW 75 °C
bis-Tris buffer/DCE=2/1
DTAB, H-G 2nd, MW 75 °C
bis-Tris buffer/DCE=2/1p-Glu
10, DTAB, H-G 2nd, MW 75 °C
bis-Tris buffer/DCE=2/1
14, DTAB, H-G 2nd, MW 75 °C
bis-Tris buffer/DCE=2/1
N
O
O
NN
Ph
n
N
N
O
O
NN
m
OHOHO
HO
O
HO
N
N
O
O
NN
k
N
O
OHOH
OH
O
OHOHO
OH
HOO
p-Glu-b-Man
4, CuSO4•5H2O,Na-ascorbate, THF/H2O
4, CuSO4•5H2O,Na-ascorbate, THF/H2O
N
O
O
N
NN
OHO
OH
HOOH
O
N
O
O
N
NN
OHO
OH
HOOH
O
N
O
O
N
NN
OHO
OH
HOOH
O
2) Ac2O, Py
1) 2-Azidoethanol,Dowex50W-X8 (H+),80 °C
2) Ac2O, Py
7 10
11 14
5
5
5
ORORO
RO
O
OR
N3
N3
O
OROR
OR
O
8 R= AcMeONa, MeOH
1
12
2
4
4
35
67
891011
12 1314
2
2
4
435
67
8
1
1
2
2
4
4
35
67
8
9
9
10
10
11
11
12
12
13
13
14
141
1
1
1
12
2
4
4
35
67
89
1011
12
13141
1
1
12
2
4
4
35
67
891011
12 1314
1
1
1
12
2
4
4
35
67
891011
12 1314
1
2
2
4
435
67
891011
1213
14
1
9 R= H
12 R= AcMeONa, MeOH
13 R= H
N
O
O
DTAB, H-G 2nd, MW 75 °C
bis-Tris buffer/DCE=2/1
DTAB, H-G 2nd, MW 75 °C
bis-Tris buffer/DCE=2/1
N
O
O
NN
n
OHOHO
HO
O
OH
N12
2
4
435
67
891011
1213
14
1
Ph
n
N
O
O
NN
N
O
OHOH
OH
O
12
2
4
4
35
67
89
101112
13141
1
Ph
p-Fuc
p-Man
p-Glc-b-Man
p-Glc-b-Fuc
p-Glc-b-Man-b-Fuc
Scheme S1. Synthetic details of homoglycopolymers (p-Glu, p-Man, p-Fuc) and multi-block glycopolymers (p-
Glu-b-Man, p- Glu-b-Fuc, p-Glu-b-Man-b-Fuc). The labels of atoms correspond with the labels of signals in figure
4.
![Page 7: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/7.jpg)
5
5. NMR spectra.
Figure S3. 1H NMR spectrum of 2-Azidoethanol.
Figure S4. 1H NMR spectrum of compound 4.
N
O
O4
N3HO
![Page 8: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/8.jpg)
6
Figure S5-1. 1H NMR spectrum of 1-azidoethyl-2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside.
Figure S5-2. 13C NMR spectrum of 1-azidoethyl-2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside.
N3OAcO
OAc
AcOOAc
O
N3OAcO
OAc
AcOOAc
O
![Page 9: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/9.jpg)
7
Figure S6-1. 1H NMR spectrum of compound 8.
Figure S6-2. 13C NMR spectrum of compound 8.
OAcOAcO
AcO
O
OAc
N38
OAcOAcO
AcO
O
OAc
N38
![Page 10: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/10.jpg)
8
Figure S7-1. 1H NMR spectrum of compound 12.
Figure S7-2. 13C NMR spectrum of compound 12.
N3
O
OAcOAcOAc
O
12
N3
O
OAcOAcOAc
O
12
![Page 11: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/11.jpg)
9
Figure S8-1. 1H NMR spectrum of monomer 5.
Figure S8-2. 13C NMR spectrum of monomer 5.
N
O
O
N
NN
OHO
OH
HOOH
O
5
N
O
O
N
NN
OHO
OH
HOOH
O
5
![Page 12: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/12.jpg)
10
Figure S8-3. COSY spectrum of monomer 5.
Figure S8-4. HSQC spectrum of monomer 5.
![Page 13: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/13.jpg)
11
Figure S9-1. 1H NMR spectrum of monomer 10.
Figure S9-2. 13C NMR spectrum of monomer 10.
NN
OHOHO
HO
O
OH
NN
O
O10
NN
OHOHO
HO
O
OH
NN
O
O10
![Page 14: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/14.jpg)
12
Figure S9-3. COSY spectrum of monomer 10.
Figure S9-4. HSQC spectrum of monomer 10.
![Page 15: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/15.jpg)
13
Figure S10-1. 1H NMR spectrum of monomer 14.
Figure S10-2. 13C NMR spectrum of monomer 14.
NN
N
O
OHOHOH
O N
O
O14
NN
N
O
OHOHOH
O N
O
O14
![Page 16: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/16.jpg)
14
Figure S10-3. COSY spectrum of monomer 14.
Figure S10-4. HSQC spectrum of monomer 14.
![Page 17: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/17.jpg)
15
Figure S11. 1H NMR spectrum of crude p-Glu (upper) and purified p-Glu (blow). The results showed that the degree of purity of glycopolymers had no influence on conversion assay based on
the 1H NMR integrations shifting from monomer olefin signals (6.36 ppm) to polymer olefin signals (5.3~5.9 ppm). Therefore, the crude glycopolymers in table 1 and table 2 were used for the
conversion assay based on 1H NMR apectrum.
Figure S12-1. 1H NMR spectrum of p-Glu in table 1, entry 1.
![Page 18: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/18.jpg)
16
Figure S12-2. 1H NMR spectrum of p-Glu in table 1, entry 2.
Figure S12-3. 1H NMR spectrum of p-Glu in table 1, entry 3.
![Page 19: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/19.jpg)
17
Figure S12-4. 1H NMR spectrum of p-Glu in table 1, entry 4.
Figure S12-5. 1H NMR spectrum of p-Glu in table 1, entry 5.
![Page 20: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/20.jpg)
18
Figure S12-6. 1H NMR spectrum of p-Glu in table 1, entry 6.
Figure S12-7. 1H NMR spectrum of p-Glu in table 1, entry 7.
![Page 21: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/21.jpg)
19
Figure S12-8. 1H NMR spectrum of p-Glu in table 1, entry 8.
Figure S12-9. 1H NMR spectrum of p-Glu in table 1, entry 9.
![Page 22: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/22.jpg)
20
Figure S12-10. 1H NMR spectrum of p-Glu in table 1, entry 10.
Figure S12-11. 1H NMR spectrum of p-Glu in table 1, entry 11.
![Page 23: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/23.jpg)
21
Figure S12-12. 1H NMR spectrum of p-Glu in table 1, entry 12.
Figure S12-13. 1H NMR spectrum of p-Glu in table 1, entry 13.
![Page 24: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/24.jpg)
22
Figure S12-14. 1H NMR spectrum of p-Glu in table 1, entry 14.
Figure S12-15. 1H NMR spectrum of p-Glu in table 1, entry 15.
![Page 25: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/25.jpg)
23
Figure S12-16. 1H NMR spectrum of p-Glu in table 1, entry 16.
Figure S12-17. 1H NMR spectrum of p-Glu in table 1, entry 17.
![Page 26: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/26.jpg)
24
Figure S12-18. 1H NMR spectrum of p-Glu in table 1, entry 18.
Figure S12-19. 1H NMR spectrum of p-Glu in table 1, entry 19.
![Page 27: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/27.jpg)
25
Figure S12-20. 1H NMR spectrum of p-Glu in table 1, entry 20.
Figure S12-21. 1H NMR spectrum of p-Glu in table 1, entry 21.
![Page 28: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/28.jpg)
26
Figure S12-22. 1H NMR spectrum of p-Glu in table 2, entry 1.
Figure S12-23. 1H NMR spectrum of p-Glu in table 2, entry 2.
![Page 29: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/29.jpg)
27
Figure S12-24. 1H NMR spectrum of p-Glu in table 2, entry 3.
Figure S12-25. 1H NMR spectrum of p-Glu in table 2, entry 4.
![Page 30: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/30.jpg)
28
Figure S12-26. 1H NMR spectrum of p-Glu in table 2, entry 5.
Figure S12-27. 1H NMR spectrum of p-Glu in table 2, entry 6.
![Page 31: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/31.jpg)
29
Figure S12-28. 1H NMR spectrum of p-Glu in table 2, entry 7.
Figure S12-29. 1H NMR spectrum of p-Glu in table 2, entry 8.
![Page 32: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/32.jpg)
30
Figure S12-30. 1H NMR spectrum of p-Glu
Figure S12-31. 1H NMR spectrum of p-Man
N
O
O
NN N
Ph
n
OHO
OH
HOOH
O
p-Glu
N
O
O
NN
n
OHOHO
HO
O
OH
N
Ph
p-Man
![Page 33: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/33.jpg)
31
Figure S12-32. 1H NMR spectrum of p-Fuc
Figure S12-33. 1H NMR spectrum of p-Glu-b-Fuc
n
N
O
O
NN
N
O
OHOH
OH
O
Ph
p-Fuc
N
O
O
NN
Ph
n
N
N
O
O
NN
m
N
O
OHOH
OH
O
OHOHO
OH
HOO
p-Glu-b-Fuc
![Page 34: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/34.jpg)
32
Figure S12-34. 1H NMR spectrum of p-Glu-b-Man
Figure S12-35. 1H NMR spectrum of p-Glu-b-Man-b-Fuc
N
O
O
NN
Ph
n
N
N
O
O
NN
m
OHOHO
HO
O
OH
N
OHOHO
OH
HOO
p-Glu-b-Man
N
O
O
NN
Ph
n
N
N
O
O
NN
m
OHOHO
HO
O
HO
N
N
O
O
NN
k
N
O
OHOH
OH
O
OHOHO
OH
HOO
p-Glu-b-Man-b-Fuc
![Page 35: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/35.jpg)
33
6. The size-exclusion chromatography (SEC) spectra.
Figure S13. The size-exclusion chromatography (SEC) spectra of crude p-Glu (a), purified p-Glu (b), and glucose monomer (c). RI: refractive index singal, LS: light scattering singal, UV: ultraviolet singal.
The results showed that the degree of purity of glycopolymers had no influence on SEC analysis, therefore crude glycopolymers in table 1and table 2 were used for SEC analysis. The monomer peak was eluted above 50 min which could be served as supplementary information of the conversion in
SEC analysis.
![Page 36: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/36.jpg)
34
![Page 37: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/37.jpg)
35
Figure S14. The size-exclusion chromatography (SEC) spectra of glycopolymers in this paper. RI: refractive index singal, LS: light scattering singal.
![Page 38: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/38.jpg)
36
7. Surface Plasmon Resonance measurements.
Figure S15. Surface plasmon resonance measurements for p-Fuc (a) and p-Glu-b-Fuc (b).
![Page 39: metathesis polymerization (ROMP) in an emulsion system Microwave-assisted … · 2017-09-12 · Electronic Supplementary Information (ESI) Microwave-assisted synthesis of glycopolymers](https://reader033.vdocument.in/reader033/viewer/2022041808/5e55fd480b8bea7ea817546b/html5/thumbnails/39.jpg)
37
Figure S16. The equilibrium response as a function of concentration from p-Glu (a), p-Man (b), p-Glu-b-Man (c) and p- Glu-b-Man-b-Fuc (d) plotted using a steady state model.