Make Growth Curve of Wild Botryococcuss braunii for each ph conditionAnalyze the growth of alga with Turbidimetry (Absorbance and %T) every 12 hoursAnalyze the growth of alga with Counting Chamber (to know how many cells of algae) every 12 hoursGive 1 mL of Walne Medium to each glassPut 21 Watt of Lamp with the distance 3 cm arround (lef, right, and above the beaker glass)Give 1 M of Citric Acid to Beaker Glass C (3,2 mL) to make medium with ph = 5Give 1 M of Citric Acid to Beaker Glass D (1.5 mL) to make medium with ph = 6Give 1 M of Citric Acid to Beaker Glass D (0.5 mL) to make medium with ph = 7Give 1 M of Citric Acid to Beaker Glass A (7mL) to make medium with ph = 3Give 1 M of Citric Acid to Beaker Glass B (5mL) to make medium with ph = 4Put microalgae and sea-water (1:4)Put the mixed of microalgae and sea water in 400 ml Beaker Glass (6 glasses)Methodology to growth wild alga in various pH

Put microalgae and sea-water (1:4)Methodology of microalgae Mutation

Mutant (microalgae that still alive)Count How many cells that still alive after the mutation processLighting microalgae with UV for 90 secondsCount How many cells of microalgae with counting chamber

Make Growth Curve of mutant Botryococcuss braunii for each ph conditionAnalyze the growth of alga with Turbidimetry (Absorbance and %T) every 12 hoursAnalyze the growth of alga with Counting Chamber (to know how many cells of algae) every 12 hoursGive 1 mL of Walne Medium to each glassPut 21 Watt of Lamp with the distance 3 cm arround (lef, right, and above the beaker glass)Give 1 M of Citric Acid to Beaker Glass C (3,2 mL) to make medium with ph = 5Give 1 M of Citric Acid to Beaker Glass D (1.5 mL) to make medium with ph = 6Give 1 M of Citric Acid to Beaker Glass D (0.5 mL) to make medium with ph = 7Give 1 M of Citric Acid to Beaker Glass A (7mL) to make medium with ph = 3Give 1 M of Citric Acid to Beaker Glass B (5mL) to make medium with ph = 4Put mutant of microalgae and sea-water (1:4)Put the mixed of mutant microalgae and sea water in 400 ml Beaker Glass (6 glasses)Methodology to growth mutant microalgaealga in various pH

Make a conclution during the result of wild and mutant comparasionAnalyze with International JournalCompare which one that can growth optimally in acid conditionGrowth Curve of mutant microalgae in various pHGrowth Curve of wild microalgae in various pHHow to compare wild and mutant microalgae