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MF-9 Ninth Microfluidics Consortium

MF-9.2

San Jose, California, USA

Final

Agenda

Speaker Profiles

Hotel and Travel Recommendations

Delegate Profiles

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The Ninth Microfluidics Consortium brings together current and future stakeholders from across a wide range of backgrounds with a shared interest in facilitating the growth of the microfluidics industry through better understanding of the challenges, opportunities and choices which it faces.

Our current membership includes: IDGG (F) ;EV Group (A) Philips (NL); Micronit (NL); Danaher (USA); Dolomite (UK) ; Fluigent (F); HP (USA); Fraunhofer (D) and Dublin City University (IRL), Labcyte (USA) ; Lonza (CH) ; Philips (NL); IMT (CH), Plastic Design Corporation (USA), HP (USA); University of California Riverside (USA) and University of Cambridge (UK).

We organize closed meetings for our members on both sides of the Atlantic where we seek to promote our mission “to grow the market for microfluidics enabled products and services” by

- Finding shared interest across the landscape of applications - Championing modularity and standards where appropriate - Engaging with key industry influencers

While helping our members to do deals along the way.

On this occasion we are delighted to be hosted by Labcyte Inc and Molecular Devices and most grateful to Yatian Qu,Thomas Carey and Don Arnold of Bay Area

Microfluidics for organizational and logistics support

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February 1 2018 (Day)

Venue: Labcyte Inc

North America HQ

170 Rose Orchard Way

San Jose, California 95134

USA (see maps in the ‘logistics’ section below)

9:00 Registration and coffee

9:30 Welcome: Mark D Fischer-Colbrie CEO Labcyte

Mr. Fischer-Colbrie became president and CEO of Labcyte in September

2008. He has more than 30 years of experience in launching novel

diagnostics, medical devices, therapeutics and high technology for growth

companies, building from start-up through IPO and into high revenue levels.

Prior to joining Labcyte, Mr. Fischer-Colbrie was senior vice president of

finance and administration and chief financial officer at Adeza Biomedical

Corporation, a women’s health care start-up company, which went public in

December 2004. The company was subsequently sold for $450 million.

During his tenure at Adeza, Mr. Fischer-Colbrie was the inventor on 4 patents and 6 patent

applications and led the business development function for three years. Mr. Fischer-Colbrie was

senior vice president, finance and administration and chief financial officer for nearly nine years at a

startup vision correction company that subsequently went public. Earlier in his career, he held several

positions including vice-president, finance, corporate controller and advanced technology business

manager at a manufacturer of disk drives that grew from a start up to $1.2 billion in revenue and also

went public.

9:45 Introductions and House Rules: Peter Hewkin CfBI

10:15 New Member Profile : University of California Riverside

Philip’s research interests comprise a variety of topics in embedded system design, especially at the intersection of processor architecture, compilers, VLSI/CAD, computer arithmetic, and reconfigurable computing. Philip joined the faculty at Riverside after being a postdoctoral scholar for three years in the Processor Architecture Laboratory in the School of Computer and Communication Sciences at the Ecole Polytechnique Fédérale de Lausanne (EPFL) in Switzerland. He received the B.S., M.S., and Ph.D. degrees, all in Computer Science, from UCLA in 2002, 2003, and 2006 respectively. He is a member of the ACM and the IEEE.

Philip has been working on several agendas which are synergistic with MF9. Today he will share with us his views about opportunities to accelerate Microfluidic R&D through CAD techniques.

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10:30 Working Session 1 - Research

Emre Araci Assistant Professor Department of Bioengineering Santa Clara University

Emre Araci has received his B.S. and M.S. degrees from Electrical

Engineering Department, Ege University, Izmir, Turkey. He received his

Ph.D. from College of Optical Sciences, University of Arizona in 2010. He

then joined Prof. Stephen Quake's group in the Bioengineering Department

at Stanford University as a postdoctoral associate and as the director of the

Stanford Microfluidics Foundry (SMF). His primary research goals are

directed toward the development and application of implantable and

miniaturized micro/optofluidic technologies for biology and medicine. His

recent work on implantable and miniaturized devices for telemedicine was

recently published in Nature Medicine and was featured in various media platforms including Fortune

magazine and ABC7. He also designed and fabricated world's smallest electromechanical valves for

mVLSI, which was published in Lab on a Chip in 2012.

Gozde Durmus, Ph.D., Stanford University “Biomarker-Free Microfluidic Technologies to Sort Circulating Rare Cells for Health and Disease”

Micro- and nano-scale technologies can have a significant impact on

medicine and biology in the areas of cell manipulation, diagnostics and

monitoring. Our research is centered on finding solutions for real world

problems at the clinic. We will present an overview of our lab's work in

magnetic levitation for assembling cells in zero-gravity and chemistry-free

sorting of rare cells from whole blood. Our levitation platform uniquely

enables ultra-sensitive density measurements, imaging, and profiling of cells

in real-time at single-cell resolution. This method has broad applications in

the label-free identification and sorting of CTCs and CTM, and in drug screening for personalized

medicine and mobile-health applications. Overall, these new technologies have the broader potential

to transform the way how patients are monitored, diagnosed, treated; and to create new paradigms

for fundamentally understanding health and disease.

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Questions / Comments

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11:30 Working Session 2: Applications

Nortis – Henning Mann VP Science ‘Organ on a Chip’

Nortis is another collaborator with Murat Cirit’s team at MIT

Nortis’ versatile microfluidic technology enables researchers to engineer a wide range of three-dimensional tissue microenvironments.

About the size of a credit card, Nortis' unique microfluidic chips can be used to grow tissue microenvironments by seeding cells into tubular voids within extracellular matrix gels. The chip design offers the flexibility to generate a variety of tissue architectures based on cell types and matrix materials selected.

Small, portable perfusion platforms house the disposable chips and supporting media and collection reservoirs. Three-shelved docking stations that reside in a standard tissue culture incubator increase experimental throughput and flexibility by enabling up to twelve independent experiments to be run at a time. Perfusion through both the tissue lumen and surrounding extralumenal space allow physico-chemical gradients to be created and perfusion fluids and cells to be collected for downstream analysis. Each docking station shelf can easily be removed and the small perfusion platform/chip assemblies accommodate easy transport between the cell culture incubator, laminar flow hood, and microscope stage.

Labcyte – Eric Hall ‘Manipulation of Droplets’

Researching new methods for genetic amplification, detection, and analysis. Current postdoctoral work is focused on the development of qPCR and RT-PCR methods in aqueous microdroplet reaciton volumes under mineral oil for sensitive single-cell detection of genes of interest. Graduate work was in the development of microfluidic platforms and protocols for single-cell analysis. My dissertation focused on the improvement of microfluidic single-cell whole-genome amplification by expanding it to a new model organism (Synechocystis cyanobacteria) which had previously proved resistant to the method. I also collaborated

12:40 Lunch/Networking/Labcyte Tour

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13:30 Working Session 3:

Microfluidics Hotseat

Pitches from early stage microfluidics

enabled companies seeking to engage with

the MF9 Consortium

Pitch 1: CombiNATi

Paul Huang

COMBiNATi's mission is to support a standard for precision personalized medicine via a widely accessible digital PCR platform. Combinati is developing a precision nucleic acid quantification platform to enable rapid and accurate genetic testing at the point of screening.

Pitch 2: Indee Labs

Ryan Pawell

Indee Labs is developing hardware for gene delivery. Our hardware is uniquely

positioned for the efficient development and scalable manufacturing of gene-

modified cell therapies

Pitch 3: Correlia Biosystems

Kursad Araz

Correlia Biosystems is creating next generation proteomic technology that

integrates complex assays such as ELISA or other immunoassays into rapid,

automated and portable platforms for pharma and diagnostic use.

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14:45 Working Session 3: DNA Read / Write

MF9 continues its quest to understand opportunities for microfluidics arising from new

techniques for “reading and writing” DNA. This stretches beyond healthcare, touching a new

set of digitally enabled industries. Today we are delighted to be addressed by:

Karin Strauss. Microsoft Research (by videolink)

Karin is a researcher in Computer Architecture at Microsoft Research (MSR). She is also an affiliate associate professor at the Department of Computer Science and Engineering at University of Washington.

Her research lies at the intersection of computer architecture, systems, and biology. As traditional semiconductor scaling has started to slow down, she investigates alternative approaches to continue the improvement we have been seeing in computer systems over the last few decades. Approaches she has been

studying include hardware accelerators for machine learning, emerging memory technologies, and the use of biotechnology to the benefit of the IT industry. She also works on system software for these new architectures.

- Lately, her focus has been on creating an end-to-end system that stores digital data in DNA. She has the privilege to work with a team of brilliant researchers and has been making quite a lot of progress.

Anup Singh – Sandia National Labs. “Synthetic Biology in a Chip: An Integrated Digital Microfluidic Platform for DNA Assembly, Transformation, and Culture”

Dr. Anup Singh is the Director of the Biological and Engineering

Sciences at Sandia National Laboratories. The center performs

exploratory science and develops technology to address pressing

national needs in energy security, homeland security, and

radiological and nuclear security. He also holds the positions of

Director of Microfluidics at the Joint BioEnergy Institute (JBEI)

located in Emeryville, CA and Adjunct Professor at University of

Texas Medical Branch in Galveston, TX.

Major areas of research in his group include: microfluidic platforms

for analysis of microbial and mammalian cells at single-cell resolution, point-of-care devices

for infectious diseases and biodefense, and microscale systems for enabling high-throughput

synthetic biology experiments.

He has published over 80 peer-reviewed publications, delivered over 150 presentations at

national and international conferences, and his inventions have led to over 40 patents and

patent applications. Many of his inventions have been licensed to companies engaged in

medical diagnostics and sensor technology development.

Discussion led by Philp Brisk – ‘implications and opportunities’ for Microfluidics at

the boundary between electronics and biology

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15:45 Working Session 4: Standards Update

The MF9 consortium continues to push for common formats/platforms, design for

manufacturing and interoperability of kit. Most recently we have focussed on infrastructure

for cell/tissue/organ on a chip. The quest continues!

At MF9.1 in Amsterdam some of the UCR work on simulating and predicting the

performance of regular networks of microfluidic links had to be redacted for IP reasons. We

hope to be able to reveal more this time.

The Consortium has been looking at standards for integrating Sample Prep into a wide range

of Dx environments. Helped by our colleagues from PurigenBio we want to run a discussion

about formats and opportunities to integrate Sample Prep in a standardized form into our

diverse products.

16:30 The Way Forward (MF9@CA)

As we increase the M9 footprint in North America, we are pleased to have stimulated

the creation of “Bay Area Microfluidics” which joins MF9 ‘cadet’ groups in

Cambridge(MA), Cambridge(US) .. and hopefully soon Paris and Montreal.

Upcoming MF9 meetings will be held in:

MF9.3 Paris March 22 & 23 Hosted by IPGG

MF9.4 / MF9.5 Boston / Montreal June 26 & 27 ..

… with FluidicMEMs (our East Coast ‘cadet’ community) joint event

Sponsored by Fluigent

MF9.6 Europe in the Fall

17:00 Close and transfer to…

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…February 1 2018 (Evening)

18:00 Joint meeting of MF9 & Bay Area Microfluidics Network

Location: Molecular Devices NEW HQ; 3860 North First Street; 95134 San Jose

(see maps in the Logistics section below)18:00 Registration, Networking –

and buffet

18:30 Thank-you to our sponsors (Fluigent and Molecular Devices)

18:40 About MF9 and Bay Area Microfluidics Network and our plans

18:50 Talks – “Microfluidics for Sequencing”

Brian Yu, Chan Zuckerberg Biohub

Dr. Brian Yu recently joined Chan Zuckerberg Biohub, where he works with faculties from Stanford, Berkeley, and UCSF to establish a microbiome initiative focused on understanding how microbial communities affect human health. Prior to joinging CZ Biohub, Brian obtained his B.S. in electrical engineering from Caltech and received his Ph.D. training at Stanford University under the joint supervision of Dr. Mark Horowitz and Dr. Stephen Quake. During his Ph.D., Brian developed a microfluidic-based metagenomic method and used it to identify novel microbial phylogenies from environmental samples. In addition to microfluidics and metagenomics, Brian was also involved in setting up

automated liquid handling capabilities for a single-cell RNA-seq pipeline and became an expert in all steps of the high-throughput sequencing process, including sample preparation, sequencing, and bioinformatics analyses. Scientifically, Brian Yu is interested in developing and optimizing system level methodologies to explore and to understand how microbial communities form, survive, evolve, and remain robust in changing environments. In particular, he believes that microfluidic technologies offer unique advantages at various sample and library preparation stages. However, simple user

experiences and robust operations are key technological challenges.

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Rajiv Bharadwaj 10X Genomics

Rajiv Bharadwaj Ph.D., is responsible for leading microfluidics programs at 10x Genomics for single cell, genomics, and exome applications. Rajiv earned his Ph.D. from Stanford University and has worked in the biotech industry for ten years with previous roles at Caliper Life Sciences.

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19:30 Interview with speakers/discussion led by Don Arnold Bay Area Microfluidics Network

Don led Eksigent’s microfluidic technology development effort, included winning an Advanced

Technologies Program grant, developing key electrokinetic flow control

technologies and managing Eksigent’s intellectual property portfolio. In 2007,

Don became VP of Business Development and Strategic Alliances and played a

key role in the sale of the analytical instruments portion of the business to AB

SCIEX in Feb 2010. In August 2010, he led the Eksigent division of AB SCIEX

as General Manager until August 2012, when he became VP of R&D and

Principal Scientist in AB SCIEX. Don transitioned to Business Development in

2015 identifying and cultivating external technologies showing potential for the SCIEX pipeline. In

2017, Don founded Veristad, LLC where he is CEO and provides expert technical and business

consulting for life science companies, assisting established companies during assessment of early-

stage technologies and assisting early-stage technology companies as the navigate from start-up to

exit

20:30 (ish) Close

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February 2 2018 - Optional Extra!!

9:00 – 11:00 Visit to WL Gore Innovation Centre. Hosted by Paul Campbell – Leader at WL

Gore and Associates.

2890 De La Cruz Blvd Santa Clara, CA 95050

Paul Campbell, an expert in corporate entrepreneurship with tenure at industry-leading companies, has repeatedly transformed corporate innovation programs to create businesses in new and adjacent markets through a deft deployment of open innovation, business accelerators, venturing, M&A, R&D, business development and strategy team leadership.

About Gore

Innovation is at the heart of our enterprise. Since 1958, Gore has pushed the envelope of materials expertise with thousands of products that change lives and industries. Whether they protect explorers atop the world’s highest peaks, travel deep into space to enable new discoveries, or work inside the human body to save and improve lives, Gore products solve complex problems and perform as promised, time and time again. And as the company that invented expanded polytetrafluoroethylene (ePTFE) — a remarkably versatile polymer that is chemically inert, lightweight and strong — we remain a worldwide fluoropolymer leader committed to collaborating closely with our

partners to solve the toughest technical challenges.

Located in Santa Clara, California, the newly opened Gore Innovation Center is where Silicon Valley explores with Gore: a space where our thoughts, skills and experiences unite, sparking new ideas and

opportunities.

This is a ‘fishing trip’ Shared interests with MF9 might include:

- Novel and precision coatings - 3D structures for organ on a chip

Outline Agenda:

9:00 – Introduction to WLGore Innovation Centre

9:40 – Tour

10:20 – Presentation about WLG Gore technologies which might be synergistic with MF9 leading to discussion about potential common interests.

11:00 – Close/Free format

If you are interested in this – contact Peter on ceo@cfbi.com for details

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MF9.2 Delegates (earlybird list)

Kusad Araz CorreliaBio

Emre Arcai USC Santa Clara

Don Arnold Veristad

Sarah Boisvert New Mexico

Philip Brisk UC Riverside

Conor Burke Fraunhofer

Paul Campbell WL Gore

Thomas Carey Berkeley

Brian Crites UC Riverside

Sammy Datwani Labcyte

Bernd Dielacher EVGroup

Gozde Dumus Stanford

Luke Ghislain Labcyte

Eric Hall Labcyte

Peter Hewkin CfBI

Paul Hung Combinati

Gretchen Kinder Plastic Design Corporation

Philip Kuhn Thermo Fischer

Darin Latimer Danaher

Henning Mann Nortis Bio

Calum Marrs Dolomite

Jeffrey McDaniel UC Riverside

David Neyer Sciex

Allen Northrup MioDX

Ryan Pawell IndeeLabs

Robert Pelletier Fluigent

Yatian Qu Purigen

Anup Singh Sandia National Labs

Karin Strauss Microsoft

Alexios Tsannis IMT

Emmet Welch Phenemenex

Mike Youngquist Molecular Devices

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Joshua Cardiel Berkeley Lights

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MF9.2 Logistics:

Delegates are responsible for making their own travel and

accommodation arrangements. Please tell us how you plan to

travel and where you will be staying so we can offer taxi/transfer

shares. The information below will facilitate this.

Overview:

San Francisco is a world class travel hub – but watch out for delays on the roads. It

is easily reached from Europe and there are lots of very good value deals at this time

of year. In particular check out Norwegian which flies from London Gatwick to

Oakland (on the other side of the Bay) for < £450.

Orientation Airports MF9/BAM Meeting MF9 Meeting

(Molecular Devices) (Labcyte)

Caltrain / VTA stop “Mountain View” WLGore Innovation Centre

^Downtown SF

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San Jose Orientation

Hotels C, A, B Labcyte Molecular Devices

VTA Light Rail Stop “Tasman”

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MF9 Consortium Daytime Meeting Venue

MF9 /BAM Evening Meeting Venue

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WL Gore Innovation Center Meeting Venue

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Recommended Hotels

There are several hotels close to our venues (see below). They are not cheap!

Further out there are cheaper options – but you will have to deal with heavy traffic at commuting

times.

See some options below:

A: Hyatt House San Jose

Walking Distance from Meeting/Evening Venues: “Comfortable, clever and inspiring, Hyatt House San

Jose/Silicon Valley offers a bold and hospitable ambiance for any length of stay. Our contemporary,

centrally located hotel is in the heart of Silicon Valley, within 10 miles of downtown San Jose and

close to great attractions like California’s Great America, Levi’s Stadium, Santa Clara Convention

Center, and the San Jose International Airport”

B: Courtyard by Marriott

Experience the stylishness and convenience of one of the newest and most modern Courtyard by

Marriott hotel in San Jose Silicon Valley, CA. In the heart of Silicon Valley, our hotel in San Jose, CA,

is located in North San Jose, just minutes from the major high tech companies in San Jose, Santa

Clara, Milpitas, Fremont, Sunnyvale and surrounding cities. Easy access to San Jose Airport, major

freeways, dining and close to attractions such as Great Mall, Great America and Levi Stadium

C: Homewood Suites by Hilton

Welcome to Homewood Suites by Hilton San Jose North. Located just off Southbay Freeway, our stylish hotel is 2 miles from Levi’s Stadium and the Santa Clara Convention Center, and close to Dell, IBM, Intel, Oracle and Samsung offices. California’s Great America and NASA Ames Research Center are 10 minutes away; San Francisco and Napa Valley are close enough for incredible day trips. Reach SJC Airport in 15 minutes and SFO Airport in 30 minutes.

Every stylish suite at our extended-stay hotel has a living area, work desk, dining space and full kitchen. Connect to complimentary internet, and relax on the sofa watching the large HDTV. Traveling with family? Our one- and two-bedroom suites will feel like home, and we’re happy to provide cribs

and high chairs to ensure our youngest guests are comfy.