michael cummings david reisman university of south carolina biotechnology and society chapter 14
TRANSCRIPT
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Michael Cummings
David Reisman • University of South Carolina
Biotechnology and Society
Chapter 14
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Biotechnology
Biotechnology • The use of recombinant DNA technology to produce
commercial goods and services• Chp13 discussed how DNA from different organisms
could be combined to create specific DNA molecules with the ability to grow in various types of cells. (1970’s)• Now, recombinant DNA technology is making a direct
impact on our everyday lives.• This has generated unresolved ethical issues.
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Biotechnology
…in this chapter
1. Pharmaceutical products made in transgenic plants and animals
2. Use of stem cells to treat disease
3. GMOs
4. Use of animals that model human diseases
5. Use of DNA in forensics and other fields
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14.1 Biopharming: Making Human Proteins in bacteria, cells, and animals Before biotechnology insulin, growth hormone, blood-clotting
factors were isolated from animals and/or human blood donations—often contaminated with HIV or hepatitis virus , some patients had a negative reaction to the animal protein.
As of 1982 insulin production utilized bacteria that were engineered to make human insulin—pure and reliable.
Blood-clotting factors made in hamster cells using recombinant DNA technology began in 1990s
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Some Products Made by Recombinant DNA Technology
Table 14-1, p. 314
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Human Proteins Can Be Made in Animals Transgenic • The transfer of genes between species
Transgenic organism• An organism that has received a gene from another
species by means of recombinant DNA technology
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Human proteins made in animals
Use recombinant DNA technology to express a human protein in the mammary glands of a cow, sheep, goat, (rabbits and hamsters used in early experiments) then patients drink the milk from these transgenic animals
• For enzyme-replacement therapy as in Pompe disease• Blood-clotting factors for hemophiliacs• Collagen• Antibodies (vaccines)
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Large Scale Synthesis of Human GAA-Pompe disease
Fig. 14-3, p. 315
Rather than using an entire animal, only animal cells are used to make a recombinant protein for medical use.
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The Use of Transgenic Plants
Gene transfer into crop plants can result in the production of human proteins
• Lower costs• Easier to grow• Using corn to make human collagen:
http://www.inpharm.com/news/161608/us-team-make-human-biocollagen-plants
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14.2 Using Stem Cells to Treat Disease
Embryonic stem cells: from the inner cell mass of early embryos. These cells are pluripotent and can form many different cell types.
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Fig. 14-4a, p. 316
Inner cell mass
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Additional Classes of Stem Cells
Adult Stem cells: recovered from bone marrow or other organs. Can develop into a limited number of mature cells, thus are considered multipotent.
Induced plutipotent stem cells (iPS): adult cells reprogrammed by transferring several master control genes into the nucleus. They behave similar to embryonic stem cells.
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Generation of Induced Puripotent Stem Cells
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Stem cell Based Therapies may Treat many Diseases Can be used to replace defective cells
Table 14-2, p. 317
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Stem cell Based Therapy for the Treatment of Burns
Fig. 14-8, p. 318
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iPS cells from patients with specific disorders Producing induced pluripotent stem cells from
individuals with genetic diseases provides a way to study disease processes, scanning drug candidates for safety and effectiveness, or application to regenerative medicine
Examples include:• Huntington’s disease• Gaucher disease• Type I juvenile diabetes
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14.3 Genetically Modified Foods
Transgenic plants are often referred to as either genetically modified organisms (GMOs) or genetically modified (GM) plants
Often more widely accepted in the US than in other nations.
Most likely over half of the food items you consumed today contained an ingredient from a GMO. 60% to 70% of foods in US supermarkets contain some transgenic plant material
Products made from corn, soybeans, cottonseed and canola oils most commonly contain transgenic ingredients
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Fig. 14-9a, p. 319
Foreign gene incorporated into a Ti plasmid
Chromosomes inside plant-cell nucleus
Bacterial chromosome
The foreign gene is transferred into a plant cell. It becomes incorporated into one of the plant’s chromosomes.1
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Fig. 14-9b, p. 319
The plant cell grows and divides. Some of the descendant cells give rise to embryos that might go on to develop into mature, genetically engineered whole plants, as below.
Embryo
2
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Transgenic Crop Plants can be made Resistant to Herbicides and Disease
Fig. 14-10, p. 320
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Transgenic Crop Plants can be made to Enhance the Nutritional Value of Foods Golden rice contains increased levels of
vitamin A
Fig. 14-11, p. 320
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Genetically Modified Crops Approved in the US
Table 14-3, p. 320
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Some Concerns About Genetically Modified Organisms
Are foods containing new proteins safe to eat?
Is it safe to eat food carrying part of a viral gene that switches on transgenes?
Can insect resistance genes be transferred to weeds or wild plants?
Can these plants have an unforeseen detrimental effect on the ecosystem?
Will pesticide-resistant insects develop?
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14.4 Transgenic Animals as Models of Human Diseases Transgenic mice are a common model system
Transfer of disease-causing human genes into mice creates mice that are used to study the development of human diseases and the effects of drugs and other therapies
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Transferring Genes into Mammals
Microinjection of fertilized eggs
Fig. 14-12, p. 322
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Mouse model for Huntington’s Disease (HD)
HD mice are extremely useful as models of human neurodegenerative disorders• Used to study the progressive destruction of brain
structures in early disease stages• Used to link changes in brain structure with changes
in behavior• Used to screen drugs to improve symptoms or
reverse brain damage
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Some Human Diseases Studied in Animal Models
Table 14-4, p. 323
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DNA Profiles
Originally, minisatellites were used to make a DNA fingerprint, now STRs are used to create a DNA profile
Short tandem repeat (STR) • Short nucleotide sequences 2 to 9 base pairs long
found throughout the human genome that organized into clusters of varying lengths
DNA profile • STR pattern used to identify individuals
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DNA Profiles Can Be Made from Short Tandem Repeats (STRs)
STRs range from 2 to 9 base pairs in length• CCTTCCCTTCCCTTCCCTTCCCTTCCCTTC
contains six repeats of the CCTTC sequence
Repeat numbers vary between individuals• A unique profile can be produced by analyzing
several STRs in a DNA sample• In the US, a standard set of 13 STRs (CODIS) is
used to prepare a profile (for more info see:
http://www.fbi.gov/about-us/lab/codis/codis-and-ndis-fact-sheet)
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A Sample DNA Profile from a Family
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DNA Profiles Are Used in the Forensics and Criminal Justice System DNA profiles are used in more than 10,000 criminal
cases per year.
Analysis of DNA profiles combines probability theory, statistics, and population genetics to estimate how frequently an allele combination is found in a population to calculate the probability that a single person will have that combination
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Fig. 14-14, p. 324
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A DNA profile from a criminal case
(Electrophoresis gel of PCR products)
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Exploring Genetics: Death of a Czar
Forensics and several types of DNA evidence were used to confirm that bones discovered in 1991 belonged to Czar Nicholas Romanov II, his wife, and three of their five children who were killed during the Russian Revolution
p. 325
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14.6 Social and Ethical Questions about Biotechnology
Applications of recombinant DNA technology have developed faster than public policy, legislation, and social norms
Some issues that have arisen include:• Should genetically modified foods be labeled?• How much do animals suffer when used to make human
proteins or used in disease models?• Who should have access to genetic information obtained from
genetic testing?• Should we test for diseases for which there is no cure yet?