michael marroquin and meg dailey. regulation of body temperature gives cells their shape major...
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EDTA Titration of Water
Michael Marroquin and Meg Dailey
Regulation of body temperature Gives cells their shape Major component of bodily fluids Keeps skin healthy Cannot store water in our bodies
Importance of Drinking Water
Classification ppm
Soft 0-17.1
Slightly Hard 17.1-60
Moderately Hard 60-120
Hard 120-180
Very Hard 180 and above
Hardness of Water
•May help towards dietary needs•Weak inverse relation between water hardness and cardiovascular disease
Determine the hardness of water in samples taken from campus apartments and dorms
Determine which building has the hardest water
Determine which building has the softest water
Determine if bottled water is more soft than samples
Purpose
Prepare EDTA solution by placing 1.8613 grams of EDTA and 8 mL of buffer in a 500 mL flask and diluting to the mark
Prepare a calcium chloride solution by placing 0.5005 grams of CaCO3 and 100 mL of 0.1 M HCl in a beaker and mixing completely
Place 3 mL of calcium chloride solution, 3 mL buffer, and 4 drops of calmagite indicator and titrate with EDTA
Determine molarity of EDTA
Procedure: Standardization of EDTA
Trial mL CaCl2 mL Buffer mL EDTA Molarity
1 3.00 3.00 14.80 0.0103
2 3.00 3.00 14.50 0.0105
3 3.00 3.00 14.60 0.0104
Average: 0.0104
Data: Standardization of EDTA
Obtain water samples from dorms and apartments on campus
Place 25 mL of water, 3 mL of buffer, and 4 drops of calgamite indicator in a 250 mL Erlenmeyer flask
Titrate water with EDTA Repeat until three good trials are obtained Repeat for each dorm and apartment
Procedure: Titration of Water
The Water Bottles
Tyler Run Apartments
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.00 50.02 ppm
2 3.00 25.00 2.80 46.68 ppm
3 3.00 25.00 2.80 46.68 ppm
4 3.00 25.00 3.00 50.02 ppm
Average: 48.35 ppm
Standard Deviation: 1.93
Data: Titration of WaterDasani Water
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 1.20 20.01
2 3.00 25.00 1.10 18.34
3 3.00 25.00 1.20 20.01
4 3.00 25.00 1.10 18.34
Average: 19.18 Standard Deviation: 0.96
Little Run Lodge
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.20 53.35 ppm
2 3.00 25.00 3.20 53.35 ppm
3 3.00 25.00 3.50 58.35 ppm
4 3.00 25.00 3.10 51.68 ppm
5 3.00 25.00 3.40 56.68 ppm
Average: 54.68 ppm
Standard Deviation: 2.74
Susquehanna Hall
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.50 58.35 ppm
2 3.00 25.00 3.70 61.69 ppm
3 3.00 25.00 3.40 56.69 ppm
Average: 58.89 ppm
Standard Deviation: 2.55
Spring Garden
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.50 58.35 ppm
2 3.00 25.00 3.80 63.36 ppm
3 3.00 25.00 3.70 61.69 ppm
4 3.00 25.00 3.50 58.35 ppm
Average: 60.425 ppm
Standard Deviation: 2.51
Beard Hall
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.10 51.68 ppm
2 3.00 25.00 3.20 53.35 ppm
3 3.00 25.00 3.30 55.02 ppm
4 3.00 25.00 3.30 55.02 ppm
Average: 53.77 ppm
Standard Devation: 1.60
Codorous Hall
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.00 50.02 ppm
2 3.00 25.00 2.95 49.18 ppm
3 3.00 25.00 3.10 51.68 ppm
4 3.00 25.00 2.90 48.35 ppm
5 3.00 25.00 3.00 50.02 ppm
Average: 49.86 ppm
Standard Deviation: 1.24
Penn Hall
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 2.80 46.68 ppm
2 3.00 25.00 2.85 47.52 ppm
3 3.00 25.00 3.00 50.02 ppm
4 3.00 25.00 2.80 46.68 ppm
Average: 47.725 ppm
Standard Deviation: 1.58
Northside Commons
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.05 50.85
2 3.00 25.00 3.15 52.52
3 3.00 25.00 3.10 51.68
Average 51.68 Standard Deviation: 1.32
Manor South
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 2.90 48.35
2 3.00 25.00 2.85 47.52
3 3.00 25.00 2.85 47.52
4 3.00 25.00 2.90 48.35
Average: 47.94 Standard Deviation: 0.48
Manor West
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 4.10 68.35
2 3.00 25.00 4.00 66.69
3 3.00 25.00 3.80 63.36
4 3.00 25.00 3.90 65.02
Average: 65.86 Standard Deviation 2.15
Manor North
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.40 56.69
2 3.00 25.00 3.60 60.02
3 3.00 25.00 3.60 60.02
Average: 58.91 Standard Deviation: 1.92
Manor Northeast
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.20 53.35
2 3.00 25.00 3.50 58.35
3 3.00 25.00 3.20 53.35
4 3.00 25.00 3.20 53.35
Average: 54.60 Standard Deviation: 2.50
Brockie Commons
Trial mL Buffer mL Water mL EDTA Hardness of Water
1 3.00 25.00 3.30 55.02
2 3.00 25.00 3.30 55.02
3 3.00 25.00 3.40 56.69
Average: 55.58 Standard Deviation: 0.96
Building Average ppm Classification
Dasani Water 19.18 Slightly Hard
Penn Hall 47.73 Slightly Hard
Manor South 48.35 Slightly Hard
Tyler Run Apartments 48.35 Slightly Hard
Codorous Hall 49.86 Slightly Hard
Northside Commons 51.68 Slightly Hard
Beard Hall 53.77 Slightly Hard
Manor Northeast 54.60 Slightly Hard
Little Run Lodge 54.68 Slightly Hard
Brockie Commons 55.58 Slightly Hard
Susquehanna Hall 58.89 Slightly Hard
Manor North 58.91 Slightly Hard
Spring Garden 60.43 Moderately Hard
Manor West 65.86 Moderately Hard
Summary of Data
Example of CalculationsHardness of Water Using Tyler Run Apartments Trial 1:
Average of Hardness of Water Using Tyler Run Apartments:
Standard Deviation of Hardness of Water Using Tyler Run Apartments:
Dasani clearly had the softest water Manor West had the hardest water Drink bottled water Had some bad trials, however, overall
experiment was successful
Conclusion