micro optical table as the new platform for a family of miniaturized optical systems: 4m device case...
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Micro Optical Table as the new platform for a Micro Optical Table as the new platform for a family of miniaturized optical systems: family of miniaturized optical systems:
4M Device case example 4M Device case example
Tomasz S. TkaczykTomasz S. Tkaczyk11, ,
EustaceEustace L. DereniakL. Dereniak11, Steve D. Gaalema, Steve D. Gaalema22, , William BahnWilliam Bahn22, Jeremy D. Rogers, Jeremy D. Rogers11, Todd C. Christenson, Todd C. Christenson33,,
Rebecca Richards-KortumRebecca Richards-Kortum44, Michael R. Descour, Michael R. Descour11
11Optical Sciences Center, University of Arizona, Tucson, AZ 85721Optical Sciences Center, University of Arizona, Tucson, AZ 8572122Black Forest Engineering, Colorado Springs, COBlack Forest Engineering, Colorado Springs, CO
33HT Micro, Albuquerque, NM 87109HT Micro, Albuquerque, NM 8710944Biomedical Engineering Program, University of Texas, Austin, TX 78712Biomedical Engineering Program, University of Texas, Austin, TX 78712
4M Device4M Device
• Miniaturized compound microscope for imaging molecular features of pre-cancer of oral cavity.
• 5.0mm(W)×13.0mm(L) ×2.5mm(H)• Battery powered and pen-sized.• Multi-modal capability:
– Reflectance imaging, – Fluorescent imaging– Optical sectioning
Folding Folding MirrorMirror
Hybrid Hybrid LensesLenses
CMOS CMOS image sensorimage sensor
BeamBeamSplitterSplitter
Condenser Condenser LensLens
LightLightSourceSource
Micro Optical Table (MOT)Micro Optical Table (MOT)Objective Objective LensLens
Object in Object in WaterWater
Sinusoidal Sinusoidal GratingGrating
Optics –TechnologyOptics –Technology
• Rotational symmetric grayscale photomask controls UV dose at given radial Rotational symmetric grayscale photomask controls UV dose at given radial distance.distance.
• Optical density is determined based on characterization curve that is Optical density is determined based on characterization curve that is experimental data.experimental data.
• It controls patterning of nearly arbitrary optical surfaces.It controls patterning of nearly arbitrary optical surfaces.
Grayscale photomask
Normalized OD vs Normalized Lens Profile
0
0.2
0.4
0.6
0.8
1
0 100 200 300 400 500 600 700 800
Radius (um)
No
rma
lize
d O
D,
Le
ns
Pro
file
Normalized OD
Lens Profile
Optics – DesignOptics – Design
• NA=0.4 to 0.7 (Object Space)NA=0.4 to 0.7 (Object Space)• Wavelength=0.60 to 0.83Wavelength=0.60 to 0.83μμmm• Full Field of View=220 to 250 Full Field of View=220 to 250 μμmm• Magnification=-4X to -7XMagnification=-4X to -7X• Working Distance=300 Working Distance=300 μμmm• Water ImmersionWater Immersion• One objective lens from Edmund optics and 3 lenslet One objective lens from Edmund optics and 3 lenslet
fabricated by photo-lithographic technique.fabricated by photo-lithographic technique.
3 equal lenslets made of sol-gel material
Water
Imaging Target
Objective Lens
Symmetric vs. Tilted SystemSymmetric vs. Tilted System
Symmetric SystemSymmetric SystemNo AR coatingsNo AR coatings
Tilted SystemTilted SystemNo AR CoatingsNo AR Coatings
System PerformanceSystem Performance
Strehl = 0.75Sag = 83.0 m
SagSag StrehlStrehl
76.076.0mm 0.800.80
80.080.0mm 0.970.97
84.084.0mm 0.820.82
Scanning MechanismScanning Mechanism
• Dimensions (screen size) – 1.3 mm Dimensions (screen size) – 1.3 mm x 1.3 mmx 1.3 mm
• Grating Frequency – 50, 60, 75, 90, Grating Frequency – 50, 60, 75, 90, 100 l/mm 100 l/mm
• Grating Profile - rectangularGrating Profile - rectangular• Scanning Freq. – 25 to 500 HzScanning Freq. – 25 to 500 Hz• Battery Powered – 1.5VBattery Powered – 1.5V• Oscillation Amplitude < Oscillation Amplitude < 100 100 mm
Signal Modulation Frequency at the Signal Modulation Frequency at the detectordetector
25 Hz to 10 kHz25 Hz to 10 kHz
Parameters SummaryParameters Summary
• OpticalOptical– Magnification = -4X to -7XMagnification = -4X to -7X– NA = 0.4 to 0.7NA = 0.4 to 0.7 = 0.60 to 0.83 = 0.60 to 0.83 mm– Field of View = 200 to 250 Field of View = 200 to 250 mm
• Structured Illumination TechniqueStructured Illumination Technique– Signal frequency (25-100Hz)Signal frequency (25-100Hz)– Number of Images for reconstruction 16 to 64Number of Images for reconstruction 16 to 64– Number of Images/cycle 3 to 5Number of Images/cycle 3 to 5
Fast CMOS detector arrayFast CMOS detector array
• 344x344 pixels344x344 pixels• 1.38x1.38 mm1.38x1.38 mm22 detection area detection area• 4 mm4 mm22 total CMOS area total CMOS area• 4x4 4x4 mm22 pixel size pixel size 50% filling factor (50% filling factor ( 3x3 3x3
mm22))• 12-bit resolution12-bit resolution• Frame Rate Frame Rate ≤≤ 500 frames/sec 500 frames/sec• RollingRolling integration integration• Operation wavelength Operation wavelength = 630 = 630
to 830 nm (830 nm is the to 830 nm (830 nm is the ultimate wavalength)ultimate wavalength)
Power specificationsPower specifications
Total chip power (worst case), mW 59.0VddA, v 3.6 54.7
Vdd, v 2.5 4.3
Supply currents, mAVddA 15.2Vdd 1.7
The final chip size is 1.52mm x 2.6mmThe final chip size is 1.52mm x 2.6mm
Column Buffer Array
Row Decoder & Pixel Reset/Enable Circuitry
Pad Names
Bonding Pads
Testing/Debugging Pads
Vdd
Pfs
Dls
Pmc
Pmod e
Vss
VssA
Vggo
Vref
Vout- D
Vout- D
Vout- C
Vout- C
Vout- B
Vout- B
Vout- A
Vout- A
VddA
Output Driver
Column Decoder
Single-ended to differential OPAMP
Single-ended to differential OPAMP
Ramp Generator
Background problem in reflectance imagingBackground problem in reflectance imaging
Images of phantoms containing SiHa cervical cancer cells labeled with anti-EGFR gold conjugates. The field of view is 54 × 54 m2. The approximate depth of the imaged optical section is 15-20 m below the phantom surface. Part (a) shows an inverted widefield reflectancemicroscope image. Part (b) shows a structured-illumination raw image. Part (c) shows a reconstructed optical-section image
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Future challengesFuture challenges
• CMOS testing and implementationCMOS testing and implementation• Integration of 4M Device Integration of 4M Device
• New optical designs and ImplementationsNew optical designs and Implementations
AcknowledgementAcknowledgement
I would like to thank my colleagues from all groups I would like to thank my colleagues from all groups participating in the project, for delivering materials participating in the project, for delivering materials
for the presentation. for the presentation.
This project is funded by NIH, NCI and NIBIBThis project is funded by NIH, NCI and NIBIB