micro-pipette motion detection by using optical fiber sensors ali fuat ergenc nejat olgac
DESCRIPTION
ALARM LAB. Micro-pipette Motion Detection by using Optical Fiber Sensors Ali Fuat Ergenc Nejat Olgac University of Connecticut, Mechanical Engineering Storrs. ALARM LAB. What is ICSI?. I ntra c ytoplasmic s perm i njection is a method of fertilization . Methods of ICSI - PowerPoint PPT PresentationTRANSCRIPT
1
Micro-pipette Motion Detection Micro-pipette Motion Detection by using Optical Fiber Sensorsby using Optical Fiber Sensors
Ali Fuat Ergenc Nejat OlgacAli Fuat Ergenc Nejat Olgac
University of Connecticut, Mechanical EngineeringUniversity of Connecticut, Mechanical Engineering
StorrsStorrs
ALARM LABALARM LAB
2
ALARM LABALARM LAB
Intracytoplasmic sperm injection is a method of fertilization .
Methods of ICSIMethods of ICSI• Conventional ICSI (success rate for mouse < 10%)
• Piezo-assisted ICSI (N/A for mouse)
• Piezo-assisted ICSI with mercury (success rate for mouse 68%)
What is ICSI?What is ICSI?
3
ALARM LABALARM LAB
Piezo-assisted ICSIPiezo-assisted ICSI
Zona penetration
Zona
Taking out the dissected zona
Oolemma Penetration
Sperm injection
4
ALARM LABALARM LAB
Manipulator drive signal
Current Feedback & Controller
Current SetupCurrent Setup
5
ALARM LABALARM LAB
Feedback?
Control signalController
-
Computer !
6
ALARM LABALARM LAB
Feedback MechanismFeedback Mechanism Current feedback mechanism is CAMERA!
• Camera is a slow feedback element.
• Image processing is a difficult way to get position feedback.
• Only 2-D position can be detected by using conventional microscope and camera
Why do we need fast feedback ?Why do we need fast feedback ?
7
ALARM LABALARM LAB
High speed movies of the pipette in the air and in High speed movies of the pipette in the air and in the dishthe dish
ALARM LABALARM LAB
• 1000 fps is used for detecting the motion
8
ALARM LABALARM LAB
Frames of the pipette in the dishFrames of the pipette in the dish
37 m
After 2 msec
30 m
116 m
Frame #2
After 1 msec
Frame #3
Frame #4
After 3 msec
25 m
Frame #6
After 5 msec
14 mFrame #5
After 4 msec
18 m
Dissected piece
Zona Zona pellucidapellucida
Frame #1
Pipette is steady 50 m
10 m
9
Frame #2
After 1 msec
270 mFrame #5
After 4 msec
100 m
Frame #6
After 5 msec
70 mFrame #1
10 m
380 m 50 m
210 m
Frame #3
After 2 msec
150 m
Frame #4
After 3 msec
ALARM LABALARM LAB
Frames of the pipette in the airFrames of the pipette in the air
10
ALARM LABALARM LAB
Motion of the Micro-Pipette Motion of the Micro-Pipette
It is shown that (Kerem Ediz, Nejat Olgac, “Micro-dynamics of the piezo-driven pipettes in ICSI”,Biomedical Engineering, IEEE Transactions on ,Volume:51)
•The flexible pipette shows extensive lateral oscillations during the piercing process.
•The amplitudes of the axial oscillations of the pipette tip are much smaller than the amplitudes of the lateral oscillations.
11
ALARM LABALARM LAB
Optical Fiber SensorOptical Fiber Sensor
It is fast,It is miniature, It is non-contact,
it can detect fast oscillations of the pipette tip.it can be attached to the holding pipette.
it doesn’t have any effect to the piercing process.
1cm
OpticalSensors
HoldingPipette
OpticalFiber
Injectionpipette
z
LASER GUN=650 nm
5mW
OpticalFiber
z
y
x
10 m 1 mm
12
ALARM LABALARM LAB
Operation Principles of the Sensor
•x-y axes position detection is obtained by voltage differentiation of quad sensors.
•z axis position detection is obtained by average light flux.
Optical Sensor
0
000
0
000
Sensor amplifier
x
z
y
0
000
0
Differential Amplifier
0
000
0
000
0
Low pass DC coupled Filter
x
z
y
y
x
13
Preliminary ExperimentsPreliminary Experiments
ALARM LABALARM LAB
Transmitter fiber
Detector fibersLaser Light
Voltage Measurements
14
ALARM LABALARM LAB
SummarySummary
Piezo impulses causes lateral oscillations at the
tip of pipette.
Camera isn’t fast enough feedback element to
capture all motions.
A new sensor is designed and preliminary
experiments are done.
15
ALARM LABALARM LAB
Future WorkFuture Work
Development of more sensitive optical detector
Miniaturization of the fiber optic detector tip
In-situ experiments and investigating the effects
of the cell on the light dispersion
The usage of the light with different wavelengths.
16
ALARM LABALARM LAB
AcknowledgementAcknowledgement
This work is partly sponsored by NIH grant, number
1 R24 RR018934-01
17
ALARM LABALARM LAB
Questions ?Questions ?